pycom14g16100

LRR receptor-like serine threonine-protein kinase FEI

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
N/A
Physical Location & Seq
Forward (+)
18510391 .. 18512916
2526 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 903 bp
ATGCAGAAGAAGGGTTTCTTGGTTTGGATTTTCTCAATAATTTCAGTTGCAATCTTCTCCTTCCATTGCTCTCTTGCTCTCAGCCAAGATGGTTTGGCATTGTTGGAAATCAAAAGCACTTTGAATGACAGCAGAAACATTCTCAGTGACTGGCAGGATTCCGATGAATCACCCTGTAATTGGACTGGCATTACTTGCCATCCTCAGGACCTTAGAGTCAGCTCTGTTAACTTGCCTTACGTGCAACTGGGTGGGACTATATCTCCCAGCATTGGTAAACTCAGTAGATTACAGAGATTGGCACTTCACCAAAACAGCTTACATGGAAACATTCCTAATGAAATTACCAATTGTGCTGAGCTTAGGGCTCTGTACTTGAGGGCTAACTACCTTCAAGGAGGTATTCCATCAGATATCGGAAACCTTTCTTCTCTCACCATACTGGATTTATCAAGCAATTTATTAAGAGGTGCTATACCTTCATCTATCGGCCGCCTCTCAAAATTACACTTTTTGAACTTGTCCACCAACTTCTTAAGCGGTGAAATCCCAGACATTGGAATTCTAAGCACTCTTGGGAACGATTCGTTTGTTGGCAATCTAGATCTTTGTGGCCAACAAGTGCACAAGCCCTGTCGGACCTCACTGGGATTTCCTGCAGTGCTACCACGTACTGCTGTAAGTGATGAAGCAGCAGCGCCCTCTAAACGATCTTCTCATTACATCAAAGGAGTGCTTATTGGTGCAATGTCTGCCTTGGGCTTTGCGTTTCTCATTCTCCTTACCTTCCTTTGGGTTCGGTCACTTTCGAAAAAGGAAAAAGTGGCTAAAAAATACACCGAAGTTAAAAAGCAAGTAAATCAAGAAACAAGTAAGTACAAAACTCATTACTTTCCATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

301

Amino Acids

32.96

Weight (kDa)

9.2

Isoelectric Point (pI)

44.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 28 - 66 1.5e-14 Leucine rich repeat N-terminal domain
LRR_14 PF23598 88 - 178 9.2e-09 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 215
AccB7I CCANNNNNTGG 1 cut(s) 557
AciI CCGC 2 cut(s) 493, 540
AcoI YGGCCR 2 cut(s) 490, 613
AcsI RAATTY 1 cut(s) 561
AfaI GTAC 3 cut(s) 374, 673, 878
AfiI CCNNNNNNNGG 5 cut(s) 180, 205, 272, 557, 792
AflII CTTAAG 1 cut(s) 535
AgsI TTSAA 3 cut(s) 124, 395, 517
AjuI GAANNNNNNNTTGG 1 cut(s) 34
AluBI AGCT 3 cut(s) 222, 318, 361
AluI AGCT 3 cut(s) 222, 318, 361
Alw21I GWGCWC 1 cut(s) 627
Alw44I GTGCAC 1 cut(s) 623
AlwNI CAGNNNCTG 1 cut(s) 150
AoxI GGCC 2 cut(s) 490, 613
ApaLI GTGCAC 1 cut(s) 623
ApeKI GCWGC 2 cut(s) 692, 695
ApoI RAATTY 1 cut(s) 561
Asp700I GAANNNNTTC 2 cut(s) 14, 424
AspLEI GCGC 1 cut(s) 700
AspS9I GGNCC 2 cut(s) 208, 639
AsuHPI GGTGA 4 cut(s) 162, 299, 427, 554
AsuII TTCGAA 1 cut(s) 809
AvaII GGWCC 2 cut(s) 208, 639
AxyI CCTNAGG 1 cut(s) 204
BaeGI GKGCMC 1 cut(s) 627
BalI TGGCCA 1 cut(s) 615
BanII GRGCYC 1 cut(s) 370
Bbv12I GWGCWC 1 cut(s) 627
BbvI GCAGC 2 cut(s) 704, 707
BccI CCATC 3 cut(s) 83, 207, 415
BfaI CTAG 1 cut(s) 602
BfmI CTRYAG 1 cut(s) 657
BfoI RGCGCY 1 cut(s) 701
BfrI CTTAAG 1 cut(s) 535
BglII AGATCT 1 cut(s) 604
BisI GCNGC 3 cut(s) 493, 693, 696
BlpI GCTNAGC 1 cut(s) 357
BlsI GCNGC 3 cut(s) 494, 694, 697
Bme18I GGWCC 2 cut(s) 208, 639
BmgT120I GGNCC 2 cut(s) 208, 639
BmrI ACTGGG 2 cut(s) 257, 656
BmuI ACTGGG 2 cut(s) 257, 656
Bpu10I CCTNAGC 1 cut(s) 362
Bpu1102I GCTNAGC 1 cut(s) 357
Bpu14I TTCGAA 1 cut(s) 809
BpuEI CTTGAG 1 cut(s) 397
BsaAI YACGTR 2 cut(s) 241, 671
BsaJI CCNNGG 2 cut(s) 756, 896
BsaXI ACNNNNNCTCC 2 cut(s) 247, 277
Bsc4I CCNNNNNNNGG 5 cut(s) 180, 205, 272, 557, 792
Bse1I ACTGG 5 cut(s) 155, 190, 252, 447, 651
Bse21I CCTNAGG 1 cut(s) 204
Bse3DI GCAATG 2 cut(s) 64, 753
BseDI CCNNGG 2 cut(s) 756, 896
BseGI GGATG 1 cut(s) 199
BseLI CCNNNNNNNGG 5 cut(s) 180, 205, 272, 557, 792
BseMI GCAATG 2 cut(s) 64, 753
BseMII CTCAG 5 cut(s) 94, 157, 218, 295, 348
BseNI ACTGG 5 cut(s) 155, 190, 252, 447, 651
BseSI GKGCMC 1 cut(s) 627
BseX3I CGGCCG 1 cut(s) 490
BseXI GCAGC 2 cut(s) 704, 707
BseYI CCCAGC 1 cut(s) 266
Bsh1285I CGRYCG 1 cut(s) 493
BshFI GGCC 2 cut(s) 492, 615
BsiEI CGRYCG 1 cut(s) 493
BsiHKAI GWGCWC 1 cut(s) 627
BslFI GGGAC 1 cut(s) 268
BslI CCNNNNNNNGG 5 cut(s) 180, 205, 272, 557, 792
BsmFI GGGAC 1 cut(s) 268
BsnI GGCC 2 cut(s) 492, 615
Bsp119I TTCGAA 1 cut(s) 809
Bsp1286I GDGCHC 2 cut(s) 370, 627
Bsp143I GATC 2 cut(s) 604, 710
Bsp1720I GCTNAGC 1 cut(s) 357
Bsp19I CCATGG 1 cut(s) 896
BspACI CCGC 2 cut(s) 493, 540
BspANI GGCC 2 cut(s) 492, 615
BspCNI CTCAG 5 cut(s) 93, 156, 217, 294, 349
BspMAI CTGCAG 1 cut(s) 661
BspT104I TTCGAA 1 cut(s) 809
BspTI CTTAAG 1 cut(s) 535
BsrDI GCAATG 2 cut(s) 64, 753
BsrI ACTGG 5 cut(s) 155, 190, 252, 447, 651
BssECI CCNNGG 2 cut(s) 756, 896
BssMI GATC 2 cut(s) 604, 710
BssT1I CCWWGG 2 cut(s) 756, 896
BstAFI CTTAAG 1 cut(s) 535
BstAPI GCANNNNNTGC 2 cut(s) 195, 752
BstBAI YACGTR 2 cut(s) 241, 671
BstBI TTCGAA 1 cut(s) 809
BstDEI CTNAG 8 cut(s) 80, 143, 204, 212, 281, 357, 362, 566
BstDSI CCRYGG 1 cut(s) 896
BstF5I GGATG 1 cut(s) 199
BstH2I RGCGCY 1 cut(s) 701
BstHHI GCGC 1 cut(s) 700
BstKTI GATC 2 cut(s) 607, 713
BstMBI GATC 2 cut(s) 604, 710
BstMCI CGRYCG 1 cut(s) 493
BstMWI GCNNNNNNNGC 3 cut(s) 195, 241, 752
BstSFI CTRYAG 1 cut(s) 657
BstSLI GKGCMC 1 cut(s) 627
BstV1I GCAGC 2 cut(s) 704, 707
BstX2I RGATCY 1 cut(s) 604
BstYI RGATCY 1 cut(s) 604
BstZI CGGCCG 1 cut(s) 490
Bsu36I CCTNAGG 1 cut(s) 204
BsuRI GGCC 2 cut(s) 492, 615
BtgI CCRYGG 1 cut(s) 896
BtsCI GGATG 1 cut(s) 199
BtsI GCAGTG 1 cut(s) 666
BtsIMutI CAGTG 3 cut(s) 151, 644, 666
CaiI CAGNNNCTG 1 cut(s) 150
CfoI GCGC 1 cut(s) 700
Cfr13I GGNCC 2 cut(s) 208, 639
Csp6I GTAC 3 cut(s) 373, 672, 877
CviAII CATG 2 cut(s) 323, 897
CviQI GTAC 3 cut(s) 373, 672, 877
DdeI CTNAG 8 cut(s) 80, 143, 204, 212, 281, 357, 362, 566
DpnI GATC 2 cut(s) 606, 712
DpnII GATC 2 cut(s) 604, 710
DrdI GACNNNNNNGTC 1 cut(s) 215
DseDI GACNNNNNNGTC 1 cut(s) 215
EaeI YGGCCR 2 cut(s) 490, 613
EagI CGGCCG 1 cut(s) 490
EclXI CGGCCG 1 cut(s) 490
Eco130I CCWWGG 2 cut(s) 756, 896
Eco24I GRGCYC 1 cut(s) 370
Eco32I GATATC 1 cut(s) 415
Eco47I GGWCC 2 cut(s) 208, 639
Eco52I CGGCCG 1 cut(s) 490
Eco81I CCTNAGG 1 cut(s) 204
EcoO109I RGGNCCY 1 cut(s) 208
EcoRI GAATTC 1 cut(s) 561
EcoRV GATATC 1 cut(s) 415
EcoT14I CCWWGG 2 cut(s) 756, 896
EcoT38I GRGCYC 1 cut(s) 370
ErhI CCWWGG 2 cut(s) 756, 896
FaeI CATG 2 cut(s) 326, 900
FaiI YATR 5 cut(s) 260, 324, 440, 476, 898
FalI AAGNNNNNCTT 3 cut(s) 34, 720, 752
FaqI GGGAC 1 cut(s) 268
FatI CATG 2 cut(s) 322, 896
Fnu4HI GCNGC 3 cut(s) 493, 693, 696
FokI GGATG 1 cut(s) 186
FriOI GRGCYC 1 cut(s) 370
Fsp4HI GCNGC 3 cut(s) 493, 693, 696
FspBI CTAG 1 cut(s) 602
GlaI GCGC 1 cut(s) 699
GluI GCNGC 3 cut(s) 493, 693, 696
GsaI CCCAGC 1 cut(s) 270
HaeII RGCGCY 1 cut(s) 701
HaeIII GGCC 2 cut(s) 492, 615
HhaI GCGC 1 cut(s) 700
Hin1II CATG 2 cut(s) 326, 900
Hin6I GCGC 1 cut(s) 698
HinP1I GCGC 1 cut(s) 698
HincII GTYRAC 1 cut(s) 229
HindII GTYRAC 1 cut(s) 229
HinfI GANTC 4 cut(s) 158, 167, 216, 584
HpaI GTTAAC 1 cut(s) 229
HphI GGTGA 4 cut(s) 162, 299, 427, 554
Hpy166II GTNNAC 4 cut(s) 229, 278, 525, 625
Hpy188I TCNGA 4 cut(s) 163, 412, 419, 639
Hpy188III TCNNGA 3 cut(s) 206, 602, 863
Hpy8I GTNNAC 4 cut(s) 229, 278, 525, 625
HpyAV CCTTC 5 cut(s) 4, 70, 401, 489, 796
HpyCH4IV ACGT 2 cut(s) 240, 670
HpyCH4V TGCA 6 cut(s) 4, 50, 244, 625, 659, 746
HpyF10VI GCNNNNNNNGC 3 cut(s) 195, 241, 752
HpyF3I CTNAG 8 cut(s) 80, 143, 204, 212, 281, 357, 362, 566
HpySE526I ACGT 2 cut(s) 240, 670
Hsp92II CATG 2 cut(s) 326, 900
HspAI GCGC 1 cut(s) 698
KspAI GTTAAC 1 cut(s) 229
Kzo9I GATC 2 cut(s) 604, 710
Lsp1109I GCAGC 2 cut(s) 704, 707
MaeI CTAG 1 cut(s) 602
MaeII ACGT 2 cut(s) 240, 670
MaeIII GTNAC 2 cut(s) 146, 801
MalI GATC 2 cut(s) 606, 712
MboI GATC 2 cut(s) 604, 710
MboII GAAGA 4 cut(s) 19, 46, 420, 705
MfeI CAATTG 1 cut(s) 349
MflI RGATCY 1 cut(s) 604
MhlI GDGCHC 2 cut(s) 370, 627
MlsI TGGCCA 1 cut(s) 615
MluCI AATT 7 cut(s) 39, 178, 342, 349, 457, 503, 561
MluNI TGGCCA 1 cut(s) 615
MlyI GAGTC 1 cut(s) 225
MmeI TCCRAC 2 cut(s) 84, 617
MnlI CCTC 7 cut(s) 213, 372, 392, 461, 506, 652, 712
Mox20I TGGCCA 1 cut(s) 615
MroXI GAANNNNTTC 2 cut(s) 14, 424
MscI TGGCCA 1 cut(s) 615
MseI TTAA 4 cut(s) 228, 464, 536, 846
Msp20I TGGCCA 1 cut(s) 615
MspCI CTTAAG 1 cut(s) 535
MunI CAATTG 1 cut(s) 349
MwoI GCNNNNNNNGC 3 cut(s) 195, 241, 752
NcoI CCATGG 1 cut(s) 896
NdeII GATC 2 cut(s) 604, 710
NlaIII CATG 2 cut(s) 326, 900
NmuCI GTSAC 2 cut(s) 146, 801
NspV TTCGAA 1 cut(s) 809
PdmI GAANNNNTTC 2 cut(s) 14, 424
PfeI GAWTC 3 cut(s) 158, 167, 584
PflMI CCANNNNNTGG 1 cut(s) 557
PkrI GCNGC 3 cut(s) 494, 694, 697
PleI GAGTC 1 cut(s) 224
PpsI GAGTC 1 cut(s) 224
Ppu21I YACGTR 2 cut(s) 241, 671
PpuMI RGGWCCY 1 cut(s) 208
Psp5II RGGWCCY 1 cut(s) 208
PspFI CCCAGC 1 cut(s) 266
PspPI GGNCC 2 cut(s) 208, 639
PspPPI RGGWCCY 1 cut(s) 208
PstI CTGCAG 1 cut(s) 661
PstNI CAGNNNCTG 1 cut(s) 150
PsuI RGATCY 1 cut(s) 604
RsaI GTAC 3 cut(s) 374, 673, 878
RsaNI GTAC 3 cut(s) 373, 672, 877
SaqAI TTAA 4 cut(s) 228, 464, 536, 846
SatI GCNGC 3 cut(s) 493, 693, 696
Sau3AI GATC 2 cut(s) 604, 710
Sau96I GGNCC 2 cut(s) 208, 639
SchI GAGTC 1 cut(s) 225
SduI GDGCHC 2 cut(s) 370, 627
SfcI CTRYAG 1 cut(s) 657
SfuI TTCGAA 1 cut(s) 809
SinI GGWCC 2 cut(s) 208, 639
SmlI CTYRAG 2 cut(s) 376, 535
SmoI CTYRAG 2 cut(s) 376, 535
Sse9I AATT 7 cut(s) 39, 178, 342, 349, 457, 503, 561
SsiI CCGC 2 cut(s) 493, 540
SspMI CTAG 1 cut(s) 602
StyI CCWWGG 2 cut(s) 756, 896
TaiI ACGT 2 cut(s) 243, 673
TaqI TCGA 1 cut(s) 809
TaqII GACCGA 1 cut(s) 789
TasI AATT 7 cut(s) 39, 178, 342, 349, 457, 503, 561
TatI WGTACW 2 cut(s) 372, 876
TauI GCSGC 1 cut(s) 495
TfiI GAWTC 3 cut(s) 158, 167, 584
Tru1I TTAA 4 cut(s) 228, 464, 536, 846
Tru9I TTAA 4 cut(s) 228, 464, 536, 846
TscAI CASTG 3 cut(s) 151, 651, 666
TseFI GTSAC 2 cut(s) 146, 801
TseI GCWGC 2 cut(s) 692, 695
Tsp45I GTSAC 2 cut(s) 146, 801
TspDTI ATGAA 4 cut(s) 180, 354, 471, 702
TspRI CASTG 3 cut(s) 151, 651, 666
Van91I CCANNNNNTGG 1 cut(s) 557
Vha464I CTTAAG 1 cut(s) 535
VneI GTGCAC 1 cut(s) 623
VpaK11BI GGWCC 2 cut(s) 208, 639
XapI RAATTY 1 cut(s) 561
XbaI TCTAGA 1 cut(s) 601
XmnI GAANNNNTTC 2 cut(s) 14, 424
XspI CTAG 1 cut(s) 602
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.