pycom14g17080

dCTP pyrophosphatase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
N/A
Physical Location & Seq
Forward (+)
19152747 .. 19153408
662 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 459 bp
ATGGGGAGTTCTCATGAGCTTGCAAGAAATGGTCATAAAGATGTTTCACTTCAAGAACTTAGTGATAGGCTTGCTGAGTTTGCTCAAGTAAGAGGATGGGAACAATATCACAGCCCCAGAAATCTCCTTCTAGCACTAGTGGGAGAGGTTGGAGAACTGTCTGAAATTTTCCAGTGGAAAGGTGAAGTTGCAAGAGGGCTGCCTGACTGGACTTCTGATGACAAAGAGCATTTGGAAGAGGAGCTCTCTGATGTTCTGCTCTACCTGATTCAACTCGCAGACGTTTGCGGACTTGATCGATCTTGGACAAGCAGCTCTCACCAAGATTGTCAAGAATGCAAGAAAATACCCCGTTGCCGAGCAAACAGCAGCTTCCACCAACTAGCTAAGCTCAAAATTAAGAACCAGCTCTATATGTTTGGATCCTCCTTTTTTTTTTTTTTTTTTTTTTCTGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00240 ko01100 map00240 map01100
Pfam Domains
Protein Families
MazG-like

Protein Analysis

153

Amino Acids

17.49

Weight (kDa)

5.47

Isoelectric Point (pI)

31.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MazG PF03819 38 - 97 1.8e-06 MazG nucleotide pyrophosphohydrolase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 288
AclWI GGATC 2 cut(s) 417, 430
AcsI RAATTY 1 cut(s) 165
AgsI TTSAA 2 cut(s) 53, 272
AhlI ACTAGT 1 cut(s) 136
AluBI AGCT 7 cut(s) 19, 244, 315, 372, 386, 391, 409
AluI AGCT 7 cut(s) 19, 244, 315, 372, 386, 391, 409
Alw21I GWGCWC 1 cut(s) 246
AlwI GGATC 2 cut(s) 417, 430
ApeKI GCWGC 3 cut(s) 199, 312, 369
ApoI RAATTY 1 cut(s) 165
AsuHPI GGTGA 2 cut(s) 194, 311
BamHI GGATCC 1 cut(s) 422
BanII GRGCYC 1 cut(s) 246
Bbv12I GWGCWC 1 cut(s) 246
BbvI GCAGC 3 cut(s) 186, 324, 381
BccI CCATC 1 cut(s) 90
BcuI ACTAGT 1 cut(s) 136
BfaI CTAG 3 cut(s) 131, 137, 383
BisI GCNGC 3 cut(s) 200, 313, 370
BlpI GCTNAGC 1 cut(s) 387
BlsI GCNGC 3 cut(s) 201, 314, 371
BmiI GGNNCC 1 cut(s) 424
BplI GAGNNNNNCTC 2 cut(s) 230, 262
Bpu1102I GCTNAGC 1 cut(s) 387
BpuEI CTTGAG 1 cut(s) 69
Bsa29I ATCGAT 1 cut(s) 298
Bse1I ACTGG 2 cut(s) 172, 212
BseCI ATCGAT 1 cut(s) 298
BseGI GGATG 1 cut(s) 101
BseMII CTCAG 1 cut(s) 66
BseNI ACTGG 2 cut(s) 172, 212
BseRI GAGGAG 1 cut(s) 254
BseXI GCAGC 3 cut(s) 186, 324, 381
BshVI ATCGAT 1 cut(s) 298
BsiHKAI GWGCWC 1 cut(s) 246
BsmI GAATGC 1 cut(s) 341
Bsp1286I GDGCHC 1 cut(s) 246
Bsp143I GATC 3 cut(s) 295, 299, 422
Bsp1720I GCTNAGC 1 cut(s) 387
BspACI CCGC 1 cut(s) 288
BspCNI CTCAG 1 cut(s) 67
BspDI ATCGAT 1 cut(s) 298
BspHI TCATGA 1 cut(s) 13
BspLI GGNNCC 1 cut(s) 424
BspPI GGATC 2 cut(s) 417, 430
BsrI ACTGG 2 cut(s) 172, 212
BssMI GATC 3 cut(s) 295, 299, 422
Bst4CI ACNGT 1 cut(s) 159
Bst6I CTCTTC 1 cut(s) 231
BstC8I GCNNGC 2 cut(s) 21, 72
BstDEI CTNAG 3 cut(s) 59, 75, 387
BstF5I GGATG 1 cut(s) 101
BstKTI GATC 3 cut(s) 298, 302, 425
BstMBI GATC 3 cut(s) 295, 299, 422
BstMWI GCNNNNNNNGC 1 cut(s) 80
BstV1I GCAGC 3 cut(s) 186, 324, 381
BstX2I RGATCY 1 cut(s) 422
BstYI RGATCY 1 cut(s) 422
Bsu15I ATCGAT 1 cut(s) 298
BsuTUI ATCGAT 1 cut(s) 298
BtsCI GGATG 1 cut(s) 101
BtsIMutI CAGTG 1 cut(s) 179
Cac8I GCNNGC 2 cut(s) 21, 72
CciI TCATGA 1 cut(s) 13
ClaI ATCGAT 1 cut(s) 298
CviAII CATG 1 cut(s) 14
DdeI CTNAG 3 cut(s) 59, 75, 387
DpnI GATC 3 cut(s) 297, 301, 424
DpnII GATC 3 cut(s) 295, 299, 422
Eam1104I CTCTTC 1 cut(s) 231
EarI CTCTTC 1 cut(s) 231
Ecl136II GAGCTC 1 cut(s) 244
Eco24I GRGCYC 1 cut(s) 246
Eco53kI GAGCTC 1 cut(s) 244
EcoICRI GAGCTC 1 cut(s) 244
EcoT38I GRGCYC 1 cut(s) 246
FaeI CATG 1 cut(s) 17
FaiI YATR 4 cut(s) 15, 36, 414, 416
FatI CATG 1 cut(s) 13
Fnu4HI GCNGC 3 cut(s) 200, 313, 370
FokI GGATG 1 cut(s) 108
FriOI GRGCYC 1 cut(s) 246
Fsp4HI GCNGC 3 cut(s) 200, 313, 370
FspBI CTAG 3 cut(s) 131, 137, 383
GluI GCNGC 3 cut(s) 200, 313, 370
Hin1II CATG 1 cut(s) 17
HinfI GANTC 1 cut(s) 268
HphI GGTGA 2 cut(s) 194, 311
Hpy188I TCNGA 3 cut(s) 163, 217, 250
Hpy188III TCNNGA 3 cut(s) 14, 53, 332
HpyAV CCTTC 1 cut(s) 137
HpyCH4III ACNGT 1 cut(s) 159
HpyCH4IV ACGT 1 cut(s) 282
HpyCH4V TGCA 3 cut(s) 23, 191, 339
HpyF10VI GCNNNNNNNGC 1 cut(s) 80
HpyF3I CTNAG 3 cut(s) 59, 75, 387
HpySE526I ACGT 1 cut(s) 282
Hsp92II CATG 1 cut(s) 17
Kzo9I GATC 3 cut(s) 295, 299, 422
LmnI GCTCC 1 cut(s) 241
LpnPI CCDG 7 cut(s) 130, 185, 193, 216, 278, 419, 438
Lsp1109I GCAGC 3 cut(s) 186, 324, 381
MaeI CTAG 3 cut(s) 131, 137, 383
MaeII ACGT 1 cut(s) 282
MalI GATC 3 cut(s) 297, 301, 424
MboI GATC 3 cut(s) 295, 299, 422
MboII GAAGA 1 cut(s) 248
MflI RGATCY 1 cut(s) 422
MhlI GDGCHC 1 cut(s) 246
MluCI AATT 2 cut(s) 165, 396
MmeI TCCRAC 1 cut(s) 130
MnlI CCTC 5 cut(s) 86, 139, 188, 232, 436
MseI TTAA 1 cut(s) 399
MslI CAYNNNNRTG 1 cut(s) 39
Mva1269I GAATGC 1 cut(s) 341
MwoI GCNNNNNNNGC 1 cut(s) 80
NdeII GATC 3 cut(s) 295, 299, 422
NlaIII CATG 1 cut(s) 17
NlaIV GGNNCC 1 cut(s) 424
NmeAIII GCCGAG 1 cut(s) 383
PagI TCATGA 1 cut(s) 13
PctI GAATGC 1 cut(s) 341
PfeI GAWTC 1 cut(s) 268
PkrI GCNGC 3 cut(s) 201, 314, 371
Psp124BI GAGCTC 1 cut(s) 246
PspN4I GGNNCC 1 cut(s) 424
PsuI RGATCY 1 cut(s) 422
RseI CAYNNNNRTG 1 cut(s) 39
SacI GAGCTC 1 cut(s) 246
SaqAI TTAA 1 cut(s) 399
SatI GCNGC 3 cut(s) 200, 313, 370
Sau3AI GATC 3 cut(s) 295, 299, 422
SduI GDGCHC 1 cut(s) 246
SmiMI CAYNNNNRTG 1 cut(s) 39
SmlI CTYRAG 1 cut(s) 84
SmoI CTYRAG 1 cut(s) 84
SpeI ACTAGT 1 cut(s) 136
Sse9I AATT 2 cut(s) 165, 396
SsiI CCGC 1 cut(s) 288
SspMI CTAG 3 cut(s) 131, 137, 383
SstI GAGCTC 1 cut(s) 246
TaaI ACNGT 1 cut(s) 159
TaiI ACGT 1 cut(s) 285
TaqI TCGA 1 cut(s) 298
TasI AATT 2 cut(s) 165, 396
TfiI GAWTC 1 cut(s) 268
Tru1I TTAA 1 cut(s) 399
Tru9I TTAA 1 cut(s) 399
TscAI CASTG 1 cut(s) 179
TseI GCWGC 3 cut(s) 199, 312, 369
TspRI CASTG 1 cut(s) 179
XapI RAATTY 1 cut(s) 165
XspI CTAG 3 cut(s) 131, 137, 383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.