pycom14g17600

Component of the replication protein A complex (RPA) required for DNA recombination, repair and replication. The activity of RPA is mediated by single-stranded DNA binding and protein interactions. Probably involved in repair of double-strand DNA breaks (DSBs) induced by genotoxic stresses

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
N/A
Physical Location & Seq
Forward (+)
19460505 .. 19461256
752 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 438 bp
ATGGCGAAGTTAATGCCGACCCCAGATGCGATTTCGACCATCTTGGCGAACAAGTCGCCGGACGAGTCGGTTGAGCTGCCGGAGATAGTGGTCCAGGTCTCCGATCTTATCCCTAGGGGAAAATCTTTCATGTTTACTGCTACTGATGGAAATGCGAAGCTAAAGGGGATGCTTTCCTCGAGGCTGGGTCCCCAGGTCACATCTGGGGAGATTCAGAATCTGGGTCTCATTCGCGTTCTCGGTTATTCTGTTAATTTCAATCCAAACTTGTCTGAAAATTATCTGCTTGTAACAAAATGTGATGTAGTTTCGGCTGCACTTGAGGCGGAGGTGAAAAATGAGGTGAAAAAAGAAGATGTTGGGATTATCTTGAAGCCCAAGCAAGAAATGGCATCGAAATCTGTTGCCCAAATTGTGCATGAGCAGGCTGGGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000228 GO:0000278 GO:0000723 GO:0000724 GO:0000725 GO:0000781 GO:0000784 GO:0000785 GO:0000790 GO:0003674 GO:0003676 GO:0003677 GO:0003697 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005662 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006268 GO:0006278 GO:0006279 GO:0006281 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007004 GO:0007049 GO:0007063 GO:0007088 GO:0007346 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010224 GO:0010556 GO:0010557 GO:0010564 GO:0010604 GO:0010638 GO:0010639 GO:0010833 GO:0010948 GO:0016043 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022402 GO:0022414 GO:0030894 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0032200 GO:0032204 GO:0032206 GO:0032210 GO:0032212 GO:0032392 GO:0032508 GO:0032879 GO:0032880 GO:0032991 GO:0032993 GO:0033043 GO:0033044 GO:0033045 GO:0033046 GO:0033047 GO:0033048 GO:0033260 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0035861 GO:0042162 GO:0042592 GO:0043047 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0043596 GO:0043601 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044786 GO:0045786 GO:0045839 GO:0045875 GO:0045930 GO:0045935 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051098 GO:0051101 GO:0051128 GO:0051129 GO:0051130 GO:0051171 GO:0051173 GO:0051276 GO:0051321 GO:0051716 GO:0051726 GO:0051783 GO:0051784 GO:0051983 GO:0051985 GO:0060249 GO:0060255 GO:0060341 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070034 GO:0071103 GO:0071704 GO:0071840 GO:0071897 GO:0071922 GO:0071923 GO:0080090 GO:0090304 GO:0090734 GO:0097159 GO:0098687 GO:0098847 GO:0106111 GO:0106112 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902969 GO:1903046 GO:1903047 GO:1903827 GO:1903828 GO:1904356 GO:1904358 GO:1905405 GO:1905412 GO:1905634 GO:2000112 GO:2000278 GO:2000573 GO:2001251 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
REPA_OB_2 Rep-A_N Rep_fac-A_C tRNA_anti-codon

Protein Analysis

146

Amino Acids

15.62

Weight (kDa)

5.86

Isoelectric Point (pI)

25.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rep-A_N PF04057 7 - 104 5e-14 Replication factor-A protein 1, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 178
AccII CGCG 1 cut(s) 234
AciI CCGC 1 cut(s) 326
AgsI TTSAA 2 cut(s) 259, 373
AjnI CCWGG 2 cut(s) 93, 192
AluBI AGCT 2 cut(s) 76, 160
AluI AGCT 2 cut(s) 76, 160
Alw26I GTCTC 2 cut(s) 103, 230
AlwNI CAGNNNCTG 1 cut(s) 220
Ama87I CYCGRG 1 cut(s) 178
ApeKI GCWGC 2 cut(s) 76, 314
AspA2I CCTAGG 1 cut(s) 113
AspS9I GGNCC 2 cut(s) 91, 188
AsuHPI GGTGA 2 cut(s) 343, 355
AvaI CYCGRG 1 cut(s) 178
AvaII GGWCC 2 cut(s) 91, 188
AvrII CCTAGG 1 cut(s) 113
BbvI GCAGC 2 cut(s) 63, 301
BccI CCATC 2 cut(s) 47, 140
BcgI CGANNNNNNTGC 1 cut(s) 29
BciT130I CCWGG 2 cut(s) 95, 194
BcoDI GTCTC 2 cut(s) 103, 230
BfaI CTAG 1 cut(s) 114
BisI GCNGC 2 cut(s) 77, 315
BlnI CCTAGG 1 cut(s) 113
BlsI GCNGC 2 cut(s) 78, 316
Bme1390I CCNGG 2 cut(s) 95, 194
Bme18I GGWCC 2 cut(s) 91, 188
BmeT110I CYCGRG 1 cut(s) 178
BmgT120I GGNCC 2 cut(s) 91, 188
BmiI GGNNCC 2 cut(s) 189, 190
BmrFI CCNGG 2 cut(s) 95, 194
BmsI GCATC 3 cut(s) 16, 159, 401
BpuEI CTTGAG 1 cut(s) 341
BsaI GGTCTC 2 cut(s) 103, 230
BsaJI CCNNGG 2 cut(s) 113, 192
BseBI CCWGG 2 cut(s) 95, 194
BseDI CCNNGG 2 cut(s) 113, 192
BseGI GGATG 1 cut(s) 174
BseXI GCAGC 2 cut(s) 63, 301
BseYI CCCAGC 2 cut(s) 184, 428
BsgI GTGCAG 1 cut(s) 300
Bsh1236I CGCG 1 cut(s) 234
BsiHKCI CYCGRG 1 cut(s) 178
BsiSI CCGG 2 cut(s) 59, 80
BslFI GGGAC 1 cut(s) 174
BsmAI GTCTC 2 cut(s) 103, 230
BsmFI GGGAC 1 cut(s) 174
Bso31I GGTCTC 2 cut(s) 103, 230
BsoBI CYCGRG 1 cut(s) 178
Bsp143I GATC 1 cut(s) 103
BspACI CCGC 1 cut(s) 326
BspFNI CGCG 1 cut(s) 234
BspLI GGNNCC 2 cut(s) 189, 190
BspTNI GGTCTC 2 cut(s) 103, 230
BssECI CCNNGG 2 cut(s) 113, 192
BssMI GATC 1 cut(s) 103
BssT1I CCWWGG 1 cut(s) 113
Bst2UI CCWGG 2 cut(s) 95, 194
BstC8I GCNNGC 1 cut(s) 426
BstF5I GGATG 1 cut(s) 174
BstFNI CGCG 1 cut(s) 234
BstKTI GATC 1 cut(s) 106
BstMAI GTCTC 2 cut(s) 103, 230
BstMBI GATC 1 cut(s) 103
BstMWI GCNNNNNNNGC 1 cut(s) 323
BstNI CCWGG 2 cut(s) 95, 194
BstSCI CCNGG 2 cut(s) 93, 192
BstUI CGCG 1 cut(s) 234
BstV1I GCAGC 2 cut(s) 63, 301
BtsCI GGATG 1 cut(s) 174
Cac8I GCNNGC 1 cut(s) 426
CaiI CAGNNNCTG 1 cut(s) 220
Cfr13I GGNCC 2 cut(s) 91, 188
CviAII CATG 2 cut(s) 130, 419
CviJI RGCY 6 cut(s) 76, 160, 184, 314, 376, 428
CviKI_1 RGCY 6 cut(s) 76, 160, 184, 314, 376, 428
DpnI GATC 1 cut(s) 105
DpnII GATC 1 cut(s) 103
EciI GGCGGA 1 cut(s) 341
Eco130I CCWWGG 1 cut(s) 113
Eco31I GGTCTC 2 cut(s) 103, 230
Eco47I GGWCC 2 cut(s) 91, 188
Eco88I CYCGRG 1 cut(s) 178
EcoO109I RGGNCCY 1 cut(s) 188
EcoRII CCWGG 2 cut(s) 93, 192
EcoT14I CCWWGG 1 cut(s) 113
ErhI CCWWGG 1 cut(s) 113
FaeI CATG 2 cut(s) 133, 422
FaiI YATR 2 cut(s) 131, 420
FaqI GGGAC 1 cut(s) 174
FatI CATG 2 cut(s) 129, 418
Fnu4HI GCNGC 2 cut(s) 77, 315
FokI GGATG 1 cut(s) 181
Fsp4HI GCNGC 2 cut(s) 77, 315
FspBI CTAG 1 cut(s) 114
GluI GCNGC 2 cut(s) 77, 315
GsaI CCCAGC 2 cut(s) 188, 432
HapII CCGG 2 cut(s) 59, 80
Hin1II CATG 2 cut(s) 133, 422
HinfI GANTC 3 cut(s) 65, 211, 217
HpaII CCGG 2 cut(s) 59, 80
HphI GGTGA 2 cut(s) 343, 355
Hpy166II GTNNAC 1 cut(s) 135
Hpy188I TCNGA 3 cut(s) 103, 216, 274
Hpy188III TCNNGA 1 cut(s) 370
Hpy8I GTNNAC 1 cut(s) 135
HpyCH4V TGCA 2 cut(s) 317, 418
HpyF10VI GCNNNNNNNGC 1 cut(s) 323
Hsp92II CATG 2 cut(s) 133, 422
KflI GGGWCCC 1 cut(s) 188
Kzo9I GATC 1 cut(s) 103
Lsp1109I GCAGC 2 cut(s) 63, 301
LweI GCATC 3 cut(s) 16, 159, 401
MaeI CTAG 1 cut(s) 114
MaeIII GTNAC 2 cut(s) 196, 289
MalI GATC 1 cut(s) 105
MboI GATC 1 cut(s) 103
MboII GAAGA 1 cut(s) 365
MluCI AATT 3 cut(s) 253, 277, 411
MlyI GAGTC 1 cut(s) 74
MnlI CCTC 5 cut(s) 174, 187, 316, 322, 334
MseI TTAA 2 cut(s) 11, 252
MspI CCGG 2 cut(s) 59, 80
MspR9I CCNGG 2 cut(s) 95, 194
MvaI CCWGG 2 cut(s) 95, 194
MvnI CGCG 1 cut(s) 234
MwoI GCNNNNNNNGC 1 cut(s) 323
NdeII GATC 1 cut(s) 103
NlaIII CATG 2 cut(s) 133, 422
NlaIV GGNNCC 2 cut(s) 189, 190
NmuCI GTSAC 1 cut(s) 196
PaeR7I CTCGAG 1 cut(s) 178
PfeI GAWTC 2 cut(s) 211, 217
PkrI GCNGC 2 cut(s) 78, 316
PleI GAGTC 1 cut(s) 73
PpsI GAGTC 1 cut(s) 73
PpuMI RGGWCCY 1 cut(s) 188
Psp5II RGGWCCY 1 cut(s) 188
Psp6I CCWGG 2 cut(s) 93, 192
PspFI CCCAGC 2 cut(s) 184, 428
PspGI CCWGG 2 cut(s) 93, 192
PspN4I GGNNCC 2 cut(s) 189, 190
PspPI GGNCC 2 cut(s) 91, 188
PspPPI RGGWCCY 1 cut(s) 188
PspXI VCTCGAGB 1 cut(s) 178
PstNI CAGNNNCTG 1 cut(s) 220
SaqAI TTAA 2 cut(s) 11, 252
SatI GCNGC 2 cut(s) 77, 315
Sau3AI GATC 1 cut(s) 103
Sau96I GGNCC 2 cut(s) 91, 188
SchI GAGTC 1 cut(s) 74
ScrFI CCNGG 2 cut(s) 95, 194
SetI ASST 6 cut(s) 78, 99, 162, 198, 333, 345
SfaNI GCATC 3 cut(s) 16, 159, 401
Sfr274I CTCGAG 1 cut(s) 178
SinI GGWCC 2 cut(s) 91, 188
SlaI CTCGAG 1 cut(s) 178
SmlI CTYRAG 2 cut(s) 178, 320
SmoI CTYRAG 2 cut(s) 178, 320
Sse9I AATT 3 cut(s) 253, 277, 411
SsiI CCGC 1 cut(s) 326
SspMI CTAG 1 cut(s) 114
StyD4I CCNGG 2 cut(s) 93, 192
StyI CCWWGG 1 cut(s) 113
TaqI TCGA 3 cut(s) 35, 179, 395
TasI AATT 3 cut(s) 253, 277, 411
TfiI GAWTC 2 cut(s) 211, 217
Tru1I TTAA 2 cut(s) 11, 252
Tru9I TTAA 2 cut(s) 11, 252
TseFI GTSAC 1 cut(s) 196
TseI GCWGC 2 cut(s) 76, 314
Tsp45I GTSAC 1 cut(s) 196
TspDTI ATGAA 1 cut(s) 118
VpaK11BI GGWCC 2 cut(s) 91, 188
XcmI CCANNNNNNNNNTGG 2 cut(s) 200, 385
XhoI CTCGAG 1 cut(s) 178
XmaJI CCTAGG 1 cut(s) 113
XspI CTAG 1 cut(s) 114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.