pycom14g18910

Peptidase M50B-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
N/A
Physical Location & Seq
Reverse (-)
20444150 .. 20445505
1356 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 651 bp
ATGAGTTACAGAGAACAACATTTTCAACTAACAAATCCCAGAAGCCCTGCGCTCCCTCGGTTCATCGCCAGGGGGACCCTGAAGGCCGTCTTTGTTCAGAATCCGAAGCACAGAGGGCGGGGCTTGAAGAATTTAGCCATCACAGCTCACCCTCTTTCCGGCTTCATTCGGCATAAAAAAAAGAGAGAAGAAATGGACTTGAATTTGTTGAAATGGAAGCCAAATTGGCAACTCAGAAAATGCTGCAACCACGACCAAGTGATCTTCCTCATCTCCGTTGCTGTCTGCACTGTCGTTATCCTTGTGCTGTGGAGGACGGTACTGATGACACCATTTAAGCTTATAACCGTGTTTCTTCACGAGGTGAGCCATGCAATCGCATGTCTGCTTACTTGTGGTAAGGTGGAAGGGATTAAGGTTAACGCAGATGAAGGTGGAGTCACACAAACCCGTGGTGGCATATCCTGGCTCATTTTGCCCGCTGGATATCTTGGTTCATCGTTCTGGGGGATGGTATTGATTCTTGCGTCTACAAAACATCTCACTACACAAATCGCTGCTGGCTGTTTCGTTGTTGCTCTAATTATTGTGTTCTTTTTAGCTAAAAATTGGACGCTTCGAGGACTTTGTATAGCACAGGATGCAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M50B

Protein Analysis

217

Amino Acids

24.17

Weight (kDa)

10.05

Isoelectric Point (pI)

24.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M50B PF13398 94 - 212 2.6e-36 Peptidase M50B-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 344
AccI GTMKAC 1 cut(s) 530
AciI CCGC 2 cut(s) 118, 480
AcsI RAATTY 2 cut(s) 130, 202
AcuI CTGAAG 1 cut(s) 101
AdeI CACNNNGTG 1 cut(s) 364
AfaI GTAC 1 cut(s) 321
AfiI CCNNNNNNNGG 1 cut(s) 158
AgsI TTSAA 4 cut(s) 26, 127, 202, 211
AjnI CCWGG 2 cut(s) 68, 464
AluBI AGCT 3 cut(s) 146, 340, 602
AluI AGCT 3 cut(s) 146, 340, 602
AoxI GGCC 1 cut(s) 84
ApeKI GCWGC 2 cut(s) 243, 557
ApoI RAATTY 2 cut(s) 130, 202
AspLEI GCGC 1 cut(s) 52
AspS9I GGNCC 1 cut(s) 75
AsuHPI GGTGA 2 cut(s) 140, 376
AvaII GGWCC 1 cut(s) 75
BauI CACGAG 1 cut(s) 359
BbvI GCAGC 2 cut(s) 230, 544
BccI CCATC 2 cut(s) 146, 505
BceAI ACGGC 1 cut(s) 71
BciT130I CCWGG 2 cut(s) 70, 466
BglI GCCNNNNNGGC 1 cut(s) 226
BisI GCNGC 2 cut(s) 244, 558
BlsI GCNGC 2 cut(s) 245, 559
Bme1390I CCNGG 2 cut(s) 70, 466
Bme18I GGWCC 1 cut(s) 75
BmgT120I GGNCC 1 cut(s) 75
BmiI GGNNCC 2 cut(s) 76, 77
BmrFI CCNGG 2 cut(s) 70, 466
BmsI GCATC 1 cut(s) 631
BsaJI CCNNGG 3 cut(s) 56, 69, 451
Bsc4I CCNNNNNNNGG 1 cut(s) 158
BseBI CCWGG 2 cut(s) 70, 466
BseDI CCNNGG 3 cut(s) 56, 69, 451
BseGI GGATG 2 cut(s) 516, 646
BseLI CCNNNNNNNGG 1 cut(s) 158
BseMII CTCAG 1 cut(s) 247
BseXI GCAGC 2 cut(s) 230, 544
BsgI GTGCAG 1 cut(s) 271
BshFI GGCC 1 cut(s) 86
BsiSI CCGG 1 cut(s) 159
BslFI GGGAC 1 cut(s) 88
BslI CCNNNNNNNGG 1 cut(s) 158
BsmFI GGGAC 1 cut(s) 88
BsnI GGCC 1 cut(s) 86
Bsp143I GATC 1 cut(s) 261
BspACI CCGC 2 cut(s) 118, 480
BspANI GGCC 1 cut(s) 86
BspCNI CTCAG 1 cut(s) 246
BspLI GGNNCC 2 cut(s) 76, 77
BssECI CCNNGG 3 cut(s) 56, 69, 451
BssMI GATC 1 cut(s) 261
BssSI CACGAG 1 cut(s) 359
Bst2BI CACGAG 1 cut(s) 359
Bst2UI CCWGG 2 cut(s) 70, 466
Bst4CI ACNGT 3 cut(s) 292, 319, 349
BstAPI GCANNNNNTGC 1 cut(s) 641
BstC8I GCNNGC 2 cut(s) 480, 562
BstDEI CTNAG 1 cut(s) 233
BstDSI CCRYGG 1 cut(s) 451
BstF5I GGATG 2 cut(s) 516, 646
BstHHI GCGC 1 cut(s) 52
BstKTI GATC 1 cut(s) 264
BstMBI GATC 1 cut(s) 261
BstMWI GCNNNNNNNGC 5 cut(s) 115, 143, 226, 475, 641
BstNI CCWGG 2 cut(s) 70, 466
BstNSI RCATGY 1 cut(s) 384
BstSCI CCNGG 2 cut(s) 68, 464
BstV1I GCAGC 2 cut(s) 230, 544
BsuRI GGCC 1 cut(s) 86
BtgI CCRYGG 1 cut(s) 451
BtgZI GCGATG 1 cut(s) 49
BtsCI GGATG 2 cut(s) 516, 646
BtsIMutI CAGTG 1 cut(s) 288
Cac8I GCNNGC 2 cut(s) 480, 562
CfoI GCGC 1 cut(s) 52
Cfr13I GGNCC 1 cut(s) 75
CseI GACGC 2 cut(s) 516, 622
Csp6I GTAC 1 cut(s) 320
CviAII CATG 2 cut(s) 371, 381
CviQI GTAC 1 cut(s) 320
DdeI CTNAG 1 cut(s) 233
DpnI GATC 1 cut(s) 263
DpnII GATC 1 cut(s) 261
DraIII CACNNNGTG 1 cut(s) 364
Eco32I GATATC 1 cut(s) 488
Eco47I GGWCC 1 cut(s) 75
Eco57I CTGAAG 1 cut(s) 101
EcoO109I RGGNCCY 1 cut(s) 75
EcoRII CCWGG 2 cut(s) 68, 464
EcoRV GATATC 1 cut(s) 488
FaeI CATG 2 cut(s) 374, 384
FaiI YATR 6 cut(s) 174, 344, 372, 382, 461, 632
FalI AAGNNNNNCTT 2 cut(s) 74, 106
FaqI GGGAC 1 cut(s) 88
FatI CATG 2 cut(s) 370, 380
FauI CCCGC 2 cut(s) 111, 487
FblI GTMKAC 1 cut(s) 530
Fnu4HI GCNGC 2 cut(s) 244, 558
FokI GGATG 1 cut(s) 523
Fsp4HI GCNGC 2 cut(s) 244, 558
GlaI GCGC 1 cut(s) 51
GluI GCNGC 2 cut(s) 244, 558
HaeIII GGCC 1 cut(s) 86
HapII CCGG 1 cut(s) 159
HgaI GACGC 2 cut(s) 516, 622
HhaI GCGC 1 cut(s) 52
Hin1II CATG 2 cut(s) 374, 384
Hin6I GCGC 1 cut(s) 50
HinP1I GCGC 1 cut(s) 50
HincII GTYRAC 1 cut(s) 421
HindII GTYRAC 1 cut(s) 421
HindIII AAGCTT 1 cut(s) 338
HinfI GANTC 3 cut(s) 100, 438, 520
HpaI GTTAAC 1 cut(s) 421
HpaII CCGG 1 cut(s) 159
HphI GGTGA 2 cut(s) 140, 376
Hpy166II GTNNAC 2 cut(s) 421, 531
Hpy188I TCNGA 3 cut(s) 99, 105, 236
Hpy188III TCNNGA 1 cut(s) 359
Hpy8I GTNNAC 2 cut(s) 421, 531
HpyAV CCTTC 3 cut(s) 76, 401, 425
HpyCH4III ACNGT 3 cut(s) 292, 319, 349
HpyCH4V TGCA 4 cut(s) 246, 288, 374, 644
HpyF10VI GCNNNNNNNGC 5 cut(s) 115, 143, 226, 475, 641
HpyF3I CTNAG 1 cut(s) 233
Hsp92II CATG 2 cut(s) 374, 384
HspAI GCGC 1 cut(s) 50
KflI GGGWCCC 1 cut(s) 75
KspAI GTTAAC 1 cut(s) 421
Kzo9I GATC 1 cut(s) 261
LmnI GCTCC 1 cut(s) 57
Lsp1109I GCAGC 2 cut(s) 230, 544
LweI GCATC 1 cut(s) 631
MaeIII GTNAC 2 cut(s) 5, 439
MalI GATC 1 cut(s) 263
MboI GATC 1 cut(s) 261
MboII GAAGA 4 cut(s) 139, 200, 256, 347
MluCI AATT 5 cut(s) 130, 202, 223, 582, 607
MlyI GAGTC 1 cut(s) 447
MnlI CCTC 7 cut(s) 66, 107, 162, 278, 306, 355, 614
MseI TTAA 3 cut(s) 336, 414, 420
MspA1I CMGCKG 1 cut(s) 482
MspI CCGG 1 cut(s) 159
MspR9I CCNGG 2 cut(s) 70, 466
MvaI CCWGG 2 cut(s) 70, 466
MwoI GCNNNNNNNGC 5 cut(s) 115, 143, 226, 475, 641
NdeII GATC 1 cut(s) 261
NlaIII CATG 2 cut(s) 374, 384
NlaIV GGNNCC 2 cut(s) 76, 77
NmuCI GTSAC 1 cut(s) 439
NspI RCATGY 1 cut(s) 384
PfeI GAWTC 2 cut(s) 100, 520
PkrI GCNGC 2 cut(s) 245, 559
PleI GAGTC 1 cut(s) 446
PpsI GAGTC 1 cut(s) 446
PpuMI RGGWCCY 1 cut(s) 75
PsiI TTATAA 1 cut(s) 344
Psp5II RGGWCCY 1 cut(s) 75
Psp6I CCWGG 2 cut(s) 68, 464
PspGI CCWGG 2 cut(s) 68, 464
PspN4I GGNNCC 2 cut(s) 76, 77
PspPI GGNCC 1 cut(s) 75
PspPPI RGGWCCY 1 cut(s) 75
RsaI GTAC 1 cut(s) 321
RsaNI GTAC 1 cut(s) 320
SaqAI TTAA 3 cut(s) 336, 414, 420
SatI GCNGC 2 cut(s) 244, 558
Sau3AI GATC 1 cut(s) 261
Sau96I GGNCC 1 cut(s) 75
SchI GAGTC 1 cut(s) 447
ScrFI CCNGG 2 cut(s) 70, 466
SetI ASST 7 cut(s) 148, 342, 366, 405, 420, 436, 604
SfaNI GCATC 1 cut(s) 631
SinI GGWCC 1 cut(s) 75
Sse9I AATT 5 cut(s) 130, 202, 223, 582, 607
SsiI CCGC 2 cut(s) 118, 480
StyD4I CCNGG 2 cut(s) 68, 464
TaaI ACNGT 3 cut(s) 292, 319, 349
TaqI TCGA 1 cut(s) 619
TasI AATT 5 cut(s) 130, 202, 223, 582, 607
TfiI GAWTC 2 cut(s) 100, 520
Tru1I TTAA 3 cut(s) 336, 414, 420
Tru9I TTAA 3 cut(s) 336, 414, 420
TscAI CASTG 1 cut(s) 295
TseFI GTSAC 1 cut(s) 439
TseI GCWGC 2 cut(s) 243, 557
Tsp45I GTSAC 1 cut(s) 439
TspDTI ATGAA 4 cut(s) 52, 154, 444, 486
TspGWI ACGGA 1 cut(s) 265
TspRI CASTG 1 cut(s) 295
VpaK11BI GGWCC 1 cut(s) 75
XapI RAATTY 2 cut(s) 130, 202
XceI RCATGY 1 cut(s) 384
XmiI GTMKAC 1 cut(s) 530
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.