pycom14g20060

Small acidic protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
N/A
Physical Location & Seq
Forward (+)
21205851 .. 21206508
658 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 336 bp
ATGTTGAGCCCTAAAATGTGTTTGCTAAACATTTTAAAACAGCTTATTTTTATAGTTTTGGATGAAAAAAAATTGAAAAGTAGAAGCAGCAAAATTGAGCTTCACAAGACCGGCTTTTTATTCACTCTTCACCAAAAAAAAAAAAAAAGCAGTGATGAGGTCGACGATGATGGACTTCTTCAAAGGCATGGAAGACCACGGCTCTACCATGGCCATGGACGTCTCGTCAACCAACTGGAGGCGTTTCGTGAGACCCAGATGGGTGAAAGCAAGCTAGTCGATGCTGACTTCTTCACCTGCTTCGAGGATGATTTCGGTGACACCAACATCAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

112

Amino Acids

12.89

Weight (kDa)

7.82

Isoelectric Point (pI)

38.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 305
AatII GACGTC 1 cut(s) 223
Acc36I ACCTGC 1 cut(s) 305
AccI GTMKAC 1 cut(s) 162
AcoI YGGCCR 1 cut(s) 211
AcyI GRCGYC 1 cut(s) 220
AfiI CCNNNNNNNGG 1 cut(s) 238
AgsI TTSAA 2 cut(s) 76, 182
AhdI GACNNNNNGTC 1 cut(s) 224
AluBI AGCT 3 cut(s) 43, 100, 274
AluI AGCT 3 cut(s) 43, 100, 274
Alw26I GTCTC 2 cut(s) 227, 245
AoxI GGCC 1 cut(s) 211
ApeKI GCWGC 1 cut(s) 87
AsuHPI GGTGA 4 cut(s) 122, 275, 286, 329
BalI TGGCCA 1 cut(s) 213
BanII GRGCYC 1 cut(s) 11
BbsI GAAGAC 1 cut(s) 199
BbvI GCAGC 1 cut(s) 99
BccI CCATC 2 cut(s) 164, 253
BceAI ACGGC 1 cut(s) 215
BcgI CGANNNNNNTGC 2 cut(s) 259, 293
BcoDI GTCTC 2 cut(s) 227, 245
BfaI CTAG 1 cut(s) 275
BfuAI ACCTGC 1 cut(s) 305
BisI GCNGC 1 cut(s) 88
BlsI GCNGC 1 cut(s) 89
BmeRI GACNNNNNGTC 1 cut(s) 224
BmsI GCATC 1 cut(s) 271
BpiI GAAGAC 1 cut(s) 199
BpmI CTGGAG 1 cut(s) 257
BsaHI GRCGYC 1 cut(s) 220
BsaI GGTCTC 1 cut(s) 245
BsaJI CCNNGG 3 cut(s) 197, 208, 214
Bsc4I CCNNNNNNNGG 1 cut(s) 238
Bse118I RCCGGY 1 cut(s) 110
Bse1I ACTGG 1 cut(s) 240
BseDI CCNNGG 3 cut(s) 197, 208, 214
BseGI GGATG 2 cut(s) 67, 313
BseLI CCNNNNNNNGG 1 cut(s) 238
BseNI ACTGG 1 cut(s) 240
BseXI GCAGC 1 cut(s) 99
BshFI GGCC 1 cut(s) 213
BsiSI CCGG 1 cut(s) 111
BslI CCNNNNNNNGG 1 cut(s) 238
BsmAI GTCTC 2 cut(s) 227, 245
BsmBI CGTCTC 1 cut(s) 227
BsnI GGCC 1 cut(s) 213
Bso31I GGTCTC 1 cut(s) 245
Bsp1286I GDGCHC 1 cut(s) 11
Bsp19I CCATGG 2 cut(s) 208, 214
BspANI GGCC 1 cut(s) 213
BspMI ACCTGC 1 cut(s) 305
BspTNI GGTCTC 1 cut(s) 245
BsrFI RCCGGY 1 cut(s) 110
BsrI ACTGG 1 cut(s) 240
BssAI RCCGGY 1 cut(s) 110
BssECI CCNNGG 3 cut(s) 197, 208, 214
BssNI GRCGYC 1 cut(s) 220
BssT1I CCWWGG 2 cut(s) 208, 214
Bst6I CTCTTC 1 cut(s) 132
BstACI GRCGYC 1 cut(s) 220
BstC8I GCNNGC 1 cut(s) 272
BstDSI CCRYGG 3 cut(s) 197, 208, 214
BstF5I GGATG 2 cut(s) 67, 313
BstMAI GTCTC 2 cut(s) 227, 245
BstV1I GCAGC 1 cut(s) 99
BstV2I GAAGAC 1 cut(s) 199
BstXI CCANNNNNNTGG 1 cut(s) 215
BsuRI GGCC 1 cut(s) 213
BtgI CCRYGG 3 cut(s) 197, 208, 214
BtsCI GGATG 2 cut(s) 67, 313
BtsI GCAGTG 1 cut(s) 157
BtsIMutI CAGTG 1 cut(s) 157
BveI ACCTGC 1 cut(s) 305
Cac8I GCNNGC 1 cut(s) 272
Cfr10I RCCGGY 1 cut(s) 110
CviAII CATG 3 cut(s) 188, 209, 215
CviJI RGCY 7 cut(s) 9, 43, 100, 114, 202, 213, 274
CviKI_1 RGCY 7 cut(s) 9, 43, 100, 114, 202, 213, 274
DraI TTTAAA 1 cut(s) 36
DriI GACNNNNNGTC 1 cut(s) 224
EaeI YGGCCR 1 cut(s) 211
Eam1104I CTCTTC 1 cut(s) 132
Eam1105I GACNNNNNGTC 1 cut(s) 224
EarI CTCTTC 1 cut(s) 132
Eco130I CCWWGG 2 cut(s) 208, 214
Eco24I GRGCYC 1 cut(s) 11
Eco31I GGTCTC 1 cut(s) 245
EcoT14I CCWWGG 2 cut(s) 208, 214
EcoT38I GRGCYC 1 cut(s) 11
ErhI CCWWGG 2 cut(s) 208, 214
Esp3I CGTCTC 1 cut(s) 227
FaeI CATG 3 cut(s) 191, 212, 218
FaiI YATR 4 cut(s) 53, 189, 210, 216
FalI AAGNNNNNCTT 2 cut(s) 98, 130
FatI CATG 3 cut(s) 187, 208, 214
FblI GTMKAC 1 cut(s) 162
Fnu4HI GCNGC 1 cut(s) 88
FokI GGATG 2 cut(s) 74, 320
FriOI GRGCYC 1 cut(s) 11
Fsp4HI GCNGC 1 cut(s) 88
FspBI CTAG 1 cut(s) 275
GluI GCNGC 1 cut(s) 88
GsuI CTGGAG 1 cut(s) 257
HaeIII GGCC 1 cut(s) 213
HapII CCGG 1 cut(s) 111
Hin1I GRCGYC 1 cut(s) 220
Hin1II CATG 3 cut(s) 191, 212, 218
HincII GTYRAC 2 cut(s) 163, 229
HindII GTYRAC 2 cut(s) 163, 229
HpaII CCGG 1 cut(s) 111
HphI GGTGA 4 cut(s) 122, 275, 286, 329
Hpy166II GTNNAC 2 cut(s) 163, 229
Hpy188III TCNNGA 1 cut(s) 248
Hpy8I GTNNAC 2 cut(s) 163, 229
Hpy99I CGWCG 1 cut(s) 167
HpyCH4IV ACGT 1 cut(s) 220
HpySE526I ACGT 1 cut(s) 220
Hsp92I GRCGYC 1 cut(s) 220
Hsp92II CATG 3 cut(s) 191, 212, 218
LpnPI CCDG 4 cut(s) 124, 221, 269, 310
Lsp1109I GCAGC 1 cut(s) 99
LweI GCATC 1 cut(s) 271
MaeI CTAG 1 cut(s) 275
MaeII ACGT 1 cut(s) 220
MaeIII GTNAC 1 cut(s) 317
MboII GAAGA 4 cut(s) 119, 170, 204, 283
MhlI GDGCHC 1 cut(s) 11
MlsI TGGCCA 1 cut(s) 213
MluCI AATT 2 cut(s) 71, 93
MluNI TGGCCA 1 cut(s) 213
MnlI CCTC 3 cut(s) 151, 232, 298
Mox20I TGGCCA 1 cut(s) 213
MscI TGGCCA 1 cut(s) 213
MseI TTAA 1 cut(s) 35
MslI CAYNNNNRTG 1 cut(s) 213
Msp20I TGGCCA 1 cut(s) 213
MspI CCGG 1 cut(s) 111
NcoI CCATGG 2 cut(s) 208, 214
NlaIII CATG 3 cut(s) 191, 212, 218
NmuCI GTSAC 1 cut(s) 317
PaqCI CACCTGC 1 cut(s) 305
PkrI GCNGC 1 cut(s) 89
RseI CAYNNNNRTG 1 cut(s) 213
SalI GTCGAC 1 cut(s) 161
SaqAI TTAA 1 cut(s) 35
SatI GCNGC 1 cut(s) 88
SduI GDGCHC 1 cut(s) 11
SetI ASST 6 cut(s) 45, 102, 162, 223, 276, 299
SfaNI GCATC 1 cut(s) 271
SmiMI CAYNNNNRTG 1 cut(s) 213
Sse9I AATT 2 cut(s) 71, 93
SspMI CTAG 1 cut(s) 275
StyI CCWWGG 2 cut(s) 208, 214
TaiI ACGT 1 cut(s) 223
TaqI TCGA 3 cut(s) 162, 279, 303
TasI AATT 2 cut(s) 71, 93
Tru1I TTAA 1 cut(s) 35
Tru9I TTAA 1 cut(s) 35
TscAI CASTG 1 cut(s) 157
TseFI GTSAC 1 cut(s) 317
TseI GCWGC 1 cut(s) 87
Tsp45I GTSAC 1 cut(s) 317
TspDTI ATGAA 1 cut(s) 78
TspRI CASTG 1 cut(s) 157
XmiI GTMKAC 1 cut(s) 162
XspI CTAG 1 cut(s) 275
ZraI GACGTC 1 cut(s) 221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.