pycom15g00360

PRONE (Plant-specific Rop nucleotide exchanger)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
N/A
Physical Location & Seq
Forward (+)
214882 .. 215503
622 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 342 bp
ATGAACAAGATTCAGTACAACAAGGATGTGGGGCAATCGATCCTTGAGAGTTACTCTAGGGTGATGGAAAGCATAGTAGATGGTAGCACCAAACAGCAGGCAGCTGCGGAGTCTACGTCTCTGTATGACCAGGGAAGGATTGGTGGCACATTGCCAAAACAGAAGCGGATATCACCCAGTCCCTTTCTCAATTCATCGTACCTCTCCATTATATTATCAGCATTGGATCCTCTTGTGAATCAGATAGGTAGAAGCCCCGGTGGAATGACATATAATAAACCAATAAAGAAGAGTAATTTTAGCGACGCGTTGGATGACAAGTTACAAAAGTTAACCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PRONE

Protein Analysis

114

Amino Acids

12.43

Weight (kDa)

9.4

Isoelectric Point (pI)

58.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRONE PF03759 1 - 25 7.5e-10 PRONE (Plant-specific Rop nucleotide exchanger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 113
AccII CGCG 1 cut(s) 308
AciI CCGC 2 cut(s) 107, 166
AclWI GGATC 3 cut(s) 34, 221, 234
AfaI GTAC 2 cut(s) 17, 200
AflIII ACRYGT 1 cut(s) 306
AjnI CCWGG 1 cut(s) 129
AluBI AGCT 1 cut(s) 104
AluI AGCT 1 cut(s) 104
Alw26I GTCTC 1 cut(s) 123
AlwI GGATC 3 cut(s) 34, 221, 234
ApeKI GCWGC 2 cut(s) 101, 104
AsuC2I CCSGG 1 cut(s) 258
AsuHPI GGTGA 2 cut(s) 73, 165
BamHI GGATCC 1 cut(s) 226
BbvI GCAGC 2 cut(s) 91, 113
BccI CCATC 2 cut(s) 58, 74
BciT130I CCWGG 1 cut(s) 131
BcnI CCSGG 1 cut(s) 258
BcoDI GTCTC 1 cut(s) 123
BfaI CTAG 1 cut(s) 57
BisI GCNGC 2 cut(s) 102, 105
BlsI GCNGC 2 cut(s) 103, 106
Bme1390I CCNGG 2 cut(s) 131, 258
BmiI GGNNCC 1 cut(s) 228
BmrFI CCNGG 2 cut(s) 131, 258
BmrI ACTGGG 1 cut(s) 171
BmuI ACTGGG 1 cut(s) 171
BplI GAGNNNNNCTC 2 cut(s) 38, 70
BpuEI CTTGAG 1 cut(s) 65
BpuMI CCSGG 1 cut(s) 258
Bsa29I ATCGAT 1 cut(s) 38
BsaJI CCNNGG 2 cut(s) 130, 256
BsaXI ACNNNNNCTCC 2 cut(s) 101, 131
Bse1I ACTGG 1 cut(s) 177
Bse3DI GCAATG 1 cut(s) 149
BseBI CCWGG 1 cut(s) 131
BseCI ATCGAT 1 cut(s) 38
BseDI CCNNGG 2 cut(s) 130, 256
BseGI GGATG 2 cut(s) 31, 319
BseMI GCAATG 1 cut(s) 149
BseNI ACTGG 1 cut(s) 177
BseXI GCAGC 2 cut(s) 91, 113
Bsh1236I CGCG 1 cut(s) 308
BshVI ATCGAT 1 cut(s) 38
BsiSI CCGG 1 cut(s) 258
BslFI GGGAC 1 cut(s) 165
BsmAI GTCTC 1 cut(s) 123
BsmBI CGTCTC 1 cut(s) 123
BsmFI GGGAC 1 cut(s) 165
Bsp143I GATC 2 cut(s) 39, 226
BspACI CCGC 2 cut(s) 107, 166
BspDI ATCGAT 1 cut(s) 38
BspFNI CGCG 1 cut(s) 308
BspLI GGNNCC 1 cut(s) 228
BspPI GGATC 3 cut(s) 34, 221, 234
BsrDI GCAATG 1 cut(s) 149
BsrI ACTGG 1 cut(s) 177
BssECI CCNNGG 2 cut(s) 130, 256
BssMI GATC 2 cut(s) 39, 226
Bst2UI CCWGG 1 cut(s) 131
Bst6I CTCTTC 1 cut(s) 284
BstC8I GCNNGC 1 cut(s) 99
BstF5I GGATG 2 cut(s) 31, 319
BstFNI CGCG 1 cut(s) 308
BstKTI GATC 2 cut(s) 42, 229
BstMAI GTCTC 1 cut(s) 123
BstMBI GATC 2 cut(s) 39, 226
BstNI CCWGG 1 cut(s) 131
BstSCI CCNGG 2 cut(s) 129, 256
BstUI CGCG 1 cut(s) 308
BstV1I GCAGC 2 cut(s) 91, 113
BstX2I RGATCY 1 cut(s) 226
BstYI RGATCY 1 cut(s) 226
Bsu15I ATCGAT 1 cut(s) 38
BsuTUI ATCGAT 1 cut(s) 38
BtsCI GGATG 2 cut(s) 31, 319
Cac8I GCNNGC 1 cut(s) 99
ClaI ATCGAT 1 cut(s) 38
CseI GACGC 1 cut(s) 314
Csp6I GTAC 2 cut(s) 16, 199
CviJI RGCY 2 cut(s) 104, 255
CviKI_1 RGCY 2 cut(s) 104, 255
CviQI GTAC 2 cut(s) 16, 199
DpnI GATC 2 cut(s) 41, 228
DpnII GATC 2 cut(s) 39, 226
Eam1104I CTCTTC 1 cut(s) 284
EarI CTCTTC 1 cut(s) 284
Eco32I GATATC 1 cut(s) 171
EcoRII CCWGG 1 cut(s) 129
EcoRV GATATC 1 cut(s) 171
Esp3I CGTCTC 1 cut(s) 123
FaiI YATR 5 cut(s) 74, 126, 212, 271, 273
FalI AAGNNNNNCTT 1 cut(s) 320
FaqI GGGAC 1 cut(s) 165
FblI GTMKAC 1 cut(s) 113
Fnu4HI GCNGC 2 cut(s) 102, 105
FokI GGATG 2 cut(s) 38, 326
Fsp4HI GCNGC 2 cut(s) 102, 105
FspBI CTAG 1 cut(s) 57
GluI GCNGC 2 cut(s) 102, 105
HapII CCGG 1 cut(s) 258
HgaI GACGC 1 cut(s) 314
HincII GTYRAC 1 cut(s) 333
HindII GTYRAC 1 cut(s) 333
HinfI GANTC 3 cut(s) 10, 110, 238
HpaI GTTAAC 1 cut(s) 333
HpaII CCGG 1 cut(s) 258
HphI GGTGA 2 cut(s) 73, 165
Hpy166II GTNNAC 2 cut(s) 114, 333
Hpy188I TCNGA 1 cut(s) 243
Hpy8I GTNNAC 2 cut(s) 114, 333
Hpy99I CGWCG 1 cut(s) 308
HpyAV CCTTC 1 cut(s) 129
HpyCH4IV ACGT 1 cut(s) 116
HpySE526I ACGT 1 cut(s) 116
KspAI GTTAAC 1 cut(s) 333
Kzo9I GATC 2 cut(s) 39, 226
LpnPI CCDG 5 cut(s) 83, 116, 143, 190, 271
Lsp1109I GCAGC 2 cut(s) 91, 113
MaeI CTAG 1 cut(s) 57
MaeII ACGT 1 cut(s) 116
MaeIII GTNAC 2 cut(s) 50, 321
MalI GATC 2 cut(s) 41, 228
MboI GATC 2 cut(s) 39, 226
MboII GAAGA 1 cut(s) 301
MflI RGATCY 1 cut(s) 226
MluCI AATT 2 cut(s) 190, 295
MluI ACGCGT 1 cut(s) 306
MlyI GAGTC 1 cut(s) 119
MmeI TCCRAC 1 cut(s) 291
MnlI CCTC 2 cut(s) 212, 240
MseI TTAA 1 cut(s) 332
MspA1I CMGCKG 1 cut(s) 104
MspI CCGG 1 cut(s) 258
MspR9I CCNGG 2 cut(s) 131, 258
MvaI CCWGG 1 cut(s) 131
MvnI CGCG 1 cut(s) 308
NciI CCSGG 1 cut(s) 258
NdeII GATC 2 cut(s) 39, 226
NlaIV GGNNCC 1 cut(s) 228
PfeI GAWTC 2 cut(s) 10, 238
PkrI GCNGC 2 cut(s) 103, 106
PleI GAGTC 1 cut(s) 118
PpsI GAGTC 1 cut(s) 118
Psp6I CCWGG 1 cut(s) 129
PspGI CCWGG 1 cut(s) 129
PspN4I GGNNCC 1 cut(s) 228
PsuI RGATCY 1 cut(s) 226
PvuII CAGCTG 1 cut(s) 104
RsaI GTAC 2 cut(s) 17, 200
RsaNI GTAC 2 cut(s) 16, 199
SaqAI TTAA 1 cut(s) 332
SatI GCNGC 2 cut(s) 102, 105
Sau3AI GATC 2 cut(s) 39, 226
SchI GAGTC 1 cut(s) 119
ScrFI CCNGG 2 cut(s) 131, 258
SetI ASST 5 cut(s) 106, 119, 204, 250, 338
SmlI CTYRAG 1 cut(s) 44
SmoI CTYRAG 1 cut(s) 44
Sse9I AATT 2 cut(s) 190, 295
SsiI CCGC 2 cut(s) 107, 166
SspMI CTAG 1 cut(s) 57
StyD4I CCNGG 2 cut(s) 129, 256
TaiI ACGT 1 cut(s) 119
TaqI TCGA 1 cut(s) 38
TasI AATT 2 cut(s) 190, 295
TatI WGTACW 1 cut(s) 15
TfiI GAWTC 2 cut(s) 10, 238
Tru1I TTAA 1 cut(s) 332
Tru9I TTAA 1 cut(s) 332
TseI GCWGC 2 cut(s) 101, 104
TspDTI ATGAA 2 cut(s) 17, 183
XcmI CCANNNNNNNNNTGG 1 cut(s) 137
XmiI GTMKAC 1 cut(s) 113
XspI CTAG 1 cut(s) 57
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.