pycom15g03030
NAC Family

Inactive protein kinase SELMODRAFT_444075-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
N/A
Physical Location & Seq
Reverse (-)
1879101 .. 1879938
838 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 579 bp
ATGGGCGGAGGCGCCGATAAAGTAATATTGGCGGTGAAAGCGGAGAAGGTGATTTCGAAAACGGCATTGGCCTGGGCGCTAACTCACGTCGGCCACCCCGGCGACTGCGTGACATTGCTTGCTGTCTTCTCCGCCATTAAAACCGGTAATTCGTTACTACTCAAGACTGTCTTTTGGATCTCCCAAAATTACGTTGTTGATCATGGAAAACCCCCAACTCCCCAAAATAGTAAAAATGACTGCGAATATGCACGATTTGATCAAGATATAAGGAAAGATAAATATGAACTGAAGGAAACCCCTAAGAAAGGCTACCTCATTAACTCTAGCATCAGGGATGCTGTTTCTCTGGGCAGAACCTCCTCAACGCTTCCCCCATTATGCTCTCTATGCCAACACAAAACACCGGTCTTTGGCAATCCTCCGAAGCAGTTCTCTTACAAAGAGTTGGAGGATGCTACAGACGCATTCTCAGATATTAACCTCTTGGAAGAAGGAGGTTTTGGTGTGGTGCACAGGGGAGTGTTAAGAGACGGTCAAGTTGTTGCTGTGAAGCAGTTGAAGTTTGGTGGATGCTGA

Protein Analysis

193

Amino Acids

20.92

Weight (kDa)

8.75

Isoelectric Point (pI)

19.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 11
AciI CCGC 4 cut(s) 6, 32, 41, 132
AclWI GGATC 1 cut(s) 185
AcoI YGGCCR 1 cut(s) 91
AcuI CTGAAG 1 cut(s) 311
AcyI GRCGYC 1 cut(s) 12
AfiI CCNNNNNNNGG 2 cut(s) 308, 413
AgeI ACCGGT 2 cut(s) 143, 406
AgsI TTSAA 1 cut(s) 562
AjiI CACGTC 1 cut(s) 88
AjnI CCWGG 1 cut(s) 71
AjuI GAANNNNNNNTTGG 4 cut(s) 50, 82, 486, 518
Alw21I GWGCWC 1 cut(s) 516
Alw26I GTCTC 1 cut(s) 525
Alw44I GTGCAC 1 cut(s) 512
AlwI GGATC 1 cut(s) 185
AoxI GGCC 2 cut(s) 69, 91
ApaLI GTGCAC 1 cut(s) 512
ArsI GACNNNNNNTTYG 2 cut(s) 393, 425
AsiGI ACCGGT 2 cut(s) 143, 406
Asp700I GAANNNNTTC 1 cut(s) 431
AspLEI GCGC 2 cut(s) 14, 79
AsuC2I CCSGG 1 cut(s) 99
AsuHPI GGTGA 2 cut(s) 46, 61
AsuII TTCGAA 1 cut(s) 56
BaeGI GKGCMC 1 cut(s) 516
BanI GGYRCC 1 cut(s) 11
BbsI GAAGAC 1 cut(s) 118
Bbv12I GWGCWC 1 cut(s) 516
BceAI ACGGC 1 cut(s) 78
BciT130I CCWGG 1 cut(s) 73
BclI TGATCA 2 cut(s) 199, 259
BcnI CCSGG 1 cut(s) 99
BcoDI GTCTC 1 cut(s) 525
BfaI CTAG 1 cut(s) 327
BfmI CTRYAG 1 cut(s) 459
BfoI RGCGCY 2 cut(s) 15, 80
BglI GCCNNNNNGGC 1 cut(s) 99
Bme1390I CCNGG 2 cut(s) 73, 99
BmgBI CACGTC 1 cut(s) 88
BmiI GGNNCC 1 cut(s) 13
BmrFI CCNGG 2 cut(s) 73, 99
BmsI GCATC 4 cut(s) 328, 339, 445, 563
BpiI GAAGAC 1 cut(s) 118
Bpu14I TTCGAA 1 cut(s) 56
BpuEI CTTGAG 1 cut(s) 146
BpuMI CCSGG 1 cut(s) 99
BsaHI GRCGYC 1 cut(s) 12
BsaJI CCNNGG 2 cut(s) 72, 97
BsaWI WCCGGW 2 cut(s) 143, 406
Bsc4I CCNNNNNNNGG 2 cut(s) 308, 413
Bse118I RCCGGY 2 cut(s) 143, 406
Bse3DI GCAATG 1 cut(s) 113
BseBI CCWGG 1 cut(s) 73
BseDI CCNNGG 2 cut(s) 72, 97
BseGI GGATG 3 cut(s) 343, 460, 578
BseLI CCNNNNNNNGG 2 cut(s) 308, 413
BseMI GCAATG 1 cut(s) 113
BseMII CTCAG 1 cut(s) 486
BseRI GAGGAG 1 cut(s) 352
BseSI GKGCMC 1 cut(s) 516
BshFI GGCC 2 cut(s) 71, 93
BshNI GGYRCC 1 cut(s) 11
BshTI ACCGGT 2 cut(s) 143, 406
BsiHKAI GWGCWC 1 cut(s) 516
BsiSI CCGG 3 cut(s) 99, 144, 407
BslI CCNNNNNNNGG 2 cut(s) 308, 413
BsmAI GTCTC 1 cut(s) 525
BsmBI CGTCTC 1 cut(s) 525
BsmI GAATGC 1 cut(s) 467
BsnI GGCC 2 cut(s) 71, 93
Bsp119I TTCGAA 1 cut(s) 56
Bsp1286I GDGCHC 1 cut(s) 516
Bsp143I GATC 3 cut(s) 177, 199, 259
BspACI CCGC 4 cut(s) 6, 32, 41, 132
BspANI GGCC 2 cut(s) 71, 93
BspCNI CTCAG 1 cut(s) 485
BspLI GGNNCC 1 cut(s) 13
BspPI GGATC 1 cut(s) 185
BspT104I TTCGAA 1 cut(s) 56
BspT107I GGYRCC 1 cut(s) 11
BsrDI GCAATG 1 cut(s) 113
BsrFI RCCGGY 2 cut(s) 143, 406
BssAI RCCGGY 2 cut(s) 143, 406
BssECI CCNNGG 2 cut(s) 72, 97
BssMI GATC 3 cut(s) 177, 199, 259
BssNI GRCGYC 1 cut(s) 12
Bst2UI CCWGG 1 cut(s) 73
Bst4CI ACNGT 2 cut(s) 169, 536
BstACI GRCGYC 1 cut(s) 12
BstBI TTCGAA 1 cut(s) 56
BstC8I GCNNGC 1 cut(s) 120
BstDEI CTNAG 2 cut(s) 303, 472
BstENI CCTNNNNNAGG 1 cut(s) 306
BstF5I GGATG 3 cut(s) 343, 460, 578
BstH2I RGCGCY 2 cut(s) 15, 80
BstHHI GCGC 2 cut(s) 14, 79
BstKTI GATC 3 cut(s) 180, 202, 262
BstMAI GTCTC 1 cut(s) 525
BstMBI GATC 3 cut(s) 177, 199, 259
BstMWI GCNNNNNNNGC 4 cut(s) 38, 99, 390, 464
BstNI CCWGG 1 cut(s) 73
BstSCI CCNGG 2 cut(s) 71, 97
BstSFI CTRYAG 1 cut(s) 459
BstSLI GKGCMC 1 cut(s) 516
BstV2I GAAGAC 1 cut(s) 118
BstX2I RGATCY 1 cut(s) 177
BstYI RGATCY 1 cut(s) 177
BsuRI GGCC 2 cut(s) 71, 93
BtrI CACGTC 1 cut(s) 88
BtsCI GGATG 3 cut(s) 343, 460, 578
Cac8I GCNNGC 1 cut(s) 120
CfoI GCGC 2 cut(s) 14, 79
Cfr10I RCCGGY 2 cut(s) 143, 406
CseI GACGC 1 cut(s) 473
CspAI ACCGGT 2 cut(s) 143, 406
CviAII CATG 1 cut(s) 203
CviJI RGCY 3 cut(s) 71, 93, 312
CviKI_1 RGCY 3 cut(s) 71, 93, 312
DdeI CTNAG 2 cut(s) 303, 472
DinI GGCGCC 1 cut(s) 13
DpnI GATC 3 cut(s) 179, 201, 261
DpnII GATC 3 cut(s) 177, 199, 259
EaeI YGGCCR 1 cut(s) 91
EciI GGCGGA 2 cut(s) 21, 121
Eco57I CTGAAG 1 cut(s) 311
EcoNI CCTNNNNNAGG 1 cut(s) 306
EcoRII CCWGG 1 cut(s) 71
EgeI GGCGCC 1 cut(s) 13
EheI GGCGCC 1 cut(s) 13
Esp3I CGTCTC 1 cut(s) 525
FaeI CATG 1 cut(s) 206
FaiI YATR 6 cut(s) 204, 249, 269, 285, 382, 391
FalI AAGNNNNNCTT 2 cut(s) 155, 187
FatI CATG 1 cut(s) 202
FbaI TGATCA 2 cut(s) 199, 259
FokI GGATG 2 cut(s) 350, 467
FspBI CTAG 1 cut(s) 327
GlaI GCGC 2 cut(s) 13, 78
HaeII RGCGCY 2 cut(s) 15, 80
HaeIII GGCC 2 cut(s) 71, 93
HapII CCGG 3 cut(s) 99, 144, 407
HgaI GACGC 1 cut(s) 473
HhaI GCGC 2 cut(s) 14, 79
Hin1I GRCGYC 1 cut(s) 12
Hin1II CATG 1 cut(s) 206
Hin6I GCGC 2 cut(s) 12, 77
HinP1I GCGC 2 cut(s) 12, 77
HpaII CCGG 3 cut(s) 99, 144, 407
HphI GGTGA 2 cut(s) 46, 61
Hpy166II GTNNAC 1 cut(s) 514
Hpy188I TCNGA 2 cut(s) 426, 475
Hpy188III TCNNGA 2 cut(s) 163, 263
Hpy8I GTNNAC 1 cut(s) 514
Hpy99I CGWCG 1 cut(s) 92
HpyAV CCTTC 3 cut(s) 40, 286, 488
HpyCH4III ACNGT 2 cut(s) 169, 536
HpyCH4IV ACGT 2 cut(s) 87, 192
HpyCH4V TGCA 2 cut(s) 251, 514
HpyF10VI GCNNNNNNNGC 4 cut(s) 38, 99, 390, 464
HpyF3I CTNAG 2 cut(s) 303, 472
HpySE526I ACGT 2 cut(s) 87, 192
Hsp92I GRCGYC 1 cut(s) 12
Hsp92II CATG 1 cut(s) 206
HspAI GCGC 2 cut(s) 12, 77
KasI GGCGCC 1 cut(s) 11
Ksp22I TGATCA 2 cut(s) 199, 259
Kzo9I GATC 3 cut(s) 177, 199, 259
LpnPI CCDG 8 cut(s) 58, 85, 112, 157, 319, 335, 420, 502
LweI GCATC 4 cut(s) 328, 339, 445, 563
MaeI CTAG 1 cut(s) 327
MaeII ACGT 2 cut(s) 87, 192
MaeIII GTNAC 2 cut(s) 109, 153
MalI GATC 3 cut(s) 179, 201, 261
MboI GATC 3 cut(s) 177, 199, 259
MboII GAAGA 2 cut(s) 118, 503
MflI RGATCY 1 cut(s) 177
MhlI GDGCHC 1 cut(s) 516
MluCI AATT 2 cut(s) 148, 187
Mly113I GGCGCC 1 cut(s) 12
MmeI TCCRAC 1 cut(s) 429
MnlI CCTC 7 cut(s) 326, 370, 373, 432, 445, 491, 494
MroXI GAANNNNTTC 1 cut(s) 431
MseI TTAA 4 cut(s) 138, 321, 480, 527
MspI CCGG 3 cut(s) 99, 144, 407
MspR9I CCNGG 2 cut(s) 73, 99
Mva1269I GAATGC 1 cut(s) 467
MvaI CCWGG 1 cut(s) 73
MwoI GCNNNNNNNGC 4 cut(s) 38, 99, 390, 464
NarI GGCGCC 1 cut(s) 12
NciI CCSGG 1 cut(s) 99
NdeII GATC 3 cut(s) 177, 199, 259
NlaIII CATG 1 cut(s) 206
NlaIV GGNNCC 1 cut(s) 13
NmuCI GTSAC 1 cut(s) 109
NspV TTCGAA 1 cut(s) 56
PctI GAATGC 1 cut(s) 467
PdmI GAANNNNTTC 1 cut(s) 431
PinAI ACCGGT 2 cut(s) 143, 406
PluTI GGCGCC 1 cut(s) 15
Psp6I CCWGG 1 cut(s) 71
PspGI CCWGG 1 cut(s) 71
PspN4I GGNNCC 1 cut(s) 13
PsuI RGATCY 1 cut(s) 177
SaqAI TTAA 4 cut(s) 138, 321, 480, 527
Sau3AI GATC 3 cut(s) 177, 199, 259
ScrFI CCNGG 2 cut(s) 73, 99
SduI GDGCHC 1 cut(s) 516
SetI ASST 7 cut(s) 51, 90, 195, 318, 362, 486, 502
SfaNI GCATC 4 cut(s) 328, 339, 445, 563
SfcI CTRYAG 1 cut(s) 459
SfoI GGCGCC 1 cut(s) 13
SfuI TTCGAA 1 cut(s) 56
SmlI CTYRAG 1 cut(s) 161
SmoI CTYRAG 1 cut(s) 161
Sse9I AATT 2 cut(s) 148, 187
SsiI CCGC 4 cut(s) 6, 32, 41, 132
SspDI GGCGCC 1 cut(s) 11
SspI AATATT 1 cut(s) 27
SspMI CTAG 1 cut(s) 327
StyD4I CCNGG 2 cut(s) 71, 97
TaaI ACNGT 2 cut(s) 169, 536
TaiI ACGT 2 cut(s) 90, 195
TaqI TCGA 1 cut(s) 56
TasI AATT 2 cut(s) 148, 187
Tru1I TTAA 4 cut(s) 138, 321, 480, 527
Tru9I TTAA 4 cut(s) 138, 321, 480, 527
TseFI GTSAC 1 cut(s) 109
Tsp45I GTSAC 1 cut(s) 109
TspDTI ATGAA 1 cut(s) 300
VneI GTGCAC 1 cut(s) 512
XagI CCTNNNNNAGG 1 cut(s) 306
XmnI GAANNNNTTC 1 cut(s) 431
XspI CTAG 1 cut(s) 327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.