pycom15g08450

Auxin-responsive protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
N/A
Physical Location & Seq
Reverse (-)
5300094 .. 5301002
909 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 558 bp
ATGGATAAGTTGTATAATATGATTTGTGAGAATAATGAAGAAGTGGTTGTGAGAGCAAATAAGAGAGGGTATGTTGCAGCAGAAGACAAAAAGCTGGAGCTGAGGCTGGGTCCTCCTGGAGGAGAAGATCGTCAGTCACTTCTCTCCCTTGGTTGCTGCAACAACAACAACAACAACAATATTCCTCATGAAGCCAAAAGAGTCTGTCATGAGGAGAAAAAAGAAGAGAGAAAGTGGTTGGCAAACAGTTCATCATCAGATCCTGCAGCTAGTGCTTCTGAACTTACGAATTCAGCTGCAGCTCCTGACTCTGATCAGAAAAGTAAAAGCAGAATTGTAAATGCTCCAGTTGTCGGGTGGCCTCCGATCCGTTCATCCAGGAAAAATCTTGCAATCAGATCATCATCATCAAGCTTCGCAAACCCGGCAGCTGATTCGGAGTCACCAAATGAAACTTCGAAGGAGGGAAATGGAAAATCTGATAGTTCTACTTATTCCAAGCACCATATGTTTGTAAAGATCGATCATGGAAGGAGTTCCCATCGGAAGAAAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
AUX_IAA

Protein Analysis

186

Amino Acids

20.32

Weight (kDa)

9.03

Isoelectric Point (pI)

58.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 32 - 175 8e-15 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 254, 361
AcsI RAATTY 1 cut(s) 289
AfiI CCNNNNNNNGG 2 cut(s) 119, 353
AjnI CCWGG 2 cut(s) 115, 377
AjuI GAANNNNNNNTTGG 2 cut(s) 439, 471
AluBI AGCT 7 cut(s) 94, 100, 269, 296, 302, 414, 431
AluI AGCT 7 cut(s) 94, 100, 269, 296, 302, 414, 431
AlwI GGATC 2 cut(s) 254, 361
AlwNI CAGNNNCTG 2 cut(s) 263, 305
AoxI GGCC 1 cut(s) 359
ApeKI GCWGC 6 cut(s) 77, 156, 266, 296, 299, 428
ApoI RAATTY 1 cut(s) 289
Asp700I GAANNNNTTC 1 cut(s) 535
AspS9I GGNCC 1 cut(s) 110
AsuC2I CCSGG 1 cut(s) 425
AsuHPI GGTGA 1 cut(s) 435
AsuII TTCGAA 1 cut(s) 458
AvaII GGWCC 1 cut(s) 110
BbsI GAAGAC 1 cut(s) 90
BbvCI CCTCAGC 1 cut(s) 101
BbvI GCAGC 6 cut(s) 89, 143, 278, 283, 311, 440
BccI CCATC 1 cut(s) 549
BcgI CGANNNNNNTGC 2 cut(s) 417, 451
BciT130I CCWGG 2 cut(s) 117, 379
BclI TGATCA 1 cut(s) 313
BcnI CCSGG 1 cut(s) 425
BfaI CTAG 1 cut(s) 270
BfmI CTRYAG 2 cut(s) 264, 297
BisI GCNGC 6 cut(s) 78, 157, 267, 297, 300, 429
BlsI GCNGC 6 cut(s) 79, 158, 268, 298, 301, 430
Bme1390I CCNGG 3 cut(s) 117, 379, 425
Bme18I GGWCC 1 cut(s) 110
BmgT120I GGNCC 1 cut(s) 110
BmiI GGNNCC 1 cut(s) 111
BmrFI CCNGG 3 cut(s) 117, 379, 425
BpiI GAAGAC 1 cut(s) 90
BpmI CTGGAG 3 cut(s) 116, 138, 330
Bpu10I CCTNAGC 1 cut(s) 101
Bpu14I TTCGAA 1 cut(s) 458
BpuMI CCSGG 1 cut(s) 425
Bsa29I ATCGAT 1 cut(s) 522
BsaBI GATNNNNATC 1 cut(s) 403
BsaJI CCNNGG 1 cut(s) 148
Bsc4I CCNNNNNNNGG 2 cut(s) 119, 353
Bse1I ACTGG 1 cut(s) 347
Bse8I GATNNNNATC 1 cut(s) 403
BseBI CCWGG 2 cut(s) 117, 379
BseCI ATCGAT 1 cut(s) 522
BseDI CCNNGG 1 cut(s) 148
BseGI GGATG 1 cut(s) 374
BseJI GATNNNNATC 1 cut(s) 403
BseLI CCNNNNNNNGG 2 cut(s) 119, 353
BseMII CTCAG 1 cut(s) 92
BseNI ACTGG 1 cut(s) 347
BseRI GAGGAG 2 cut(s) 135, 227
BseXI GCAGC 6 cut(s) 89, 143, 278, 283, 311, 440
BseYI CCCAGC 1 cut(s) 106
BshFI GGCC 1 cut(s) 361
BshVI ATCGAT 1 cut(s) 522
BsiSI CCGG 1 cut(s) 425
BslI CCNNNNNNNGG 2 cut(s) 119, 353
BsnI GGCC 1 cut(s) 361
Bsp119I TTCGAA 1 cut(s) 458
Bsp143I GATC 7 cut(s) 127, 259, 313, 366, 398, 519, 523
BspANI GGCC 1 cut(s) 361
BspCNI CTCAG 1 cut(s) 93
BspDI ATCGAT 1 cut(s) 522
BspHI TCATGA 2 cut(s) 187, 208
BspLI GGNNCC 1 cut(s) 111
BspMAI CTGCAG 2 cut(s) 268, 301
BspPI GGATC 2 cut(s) 254, 361
BspT104I TTCGAA 1 cut(s) 458
BsrI ACTGG 1 cut(s) 347
BssECI CCNNGG 1 cut(s) 148
BssMI GATC 7 cut(s) 127, 259, 313, 366, 398, 519, 523
BssT1I CCWWGG 1 cut(s) 148
Bst2UI CCWGG 2 cut(s) 117, 379
Bst4CI ACNGT 1 cut(s) 248
Bst6I CTCTTC 1 cut(s) 219
BstAPI GCANNNNNTGC 1 cut(s) 272
BstBI TTCGAA 1 cut(s) 458
BstDEI CTNAG 1 cut(s) 101
BstENI CCTNNNNNAGG 1 cut(s) 117
BstF5I GGATG 1 cut(s) 374
BstKTI GATC 7 cut(s) 130, 262, 316, 369, 401, 522, 526
BstMBI GATC 7 cut(s) 127, 259, 313, 366, 398, 519, 523
BstMWI GCNNNNNNNGC 2 cut(s) 272, 425
BstNI CCWGG 2 cut(s) 117, 379
BstSCI CCNGG 3 cut(s) 115, 377, 423
BstSFI CTRYAG 2 cut(s) 264, 297
BstV1I GCAGC 6 cut(s) 89, 143, 278, 283, 311, 440
BstV2I GAAGAC 1 cut(s) 90
BstX2I RGATCY 1 cut(s) 259
BstYI RGATCY 1 cut(s) 259
Bsu15I ATCGAT 1 cut(s) 522
BsuRI GGCC 1 cut(s) 361
BsuTUI ATCGAT 1 cut(s) 522
BtsCI GGATG 1 cut(s) 374
CaiI CAGNNNCTG 2 cut(s) 263, 305
CciI TCATGA 2 cut(s) 187, 208
Cfr13I GGNCC 1 cut(s) 110
ClaI ATCGAT 1 cut(s) 522
CviAII CATG 3 cut(s) 188, 209, 527
DdeI CTNAG 1 cut(s) 101
DpnI GATC 7 cut(s) 129, 261, 315, 368, 400, 521, 525
DpnII GATC 7 cut(s) 127, 259, 313, 366, 398, 519, 523
Eam1104I CTCTTC 1 cut(s) 219
EarI CTCTTC 1 cut(s) 219
Eco130I CCWWGG 1 cut(s) 148
Eco47I GGWCC 1 cut(s) 110
EcoNI CCTNNNNNAGG 1 cut(s) 117
EcoO109I RGGNCCY 1 cut(s) 110
EcoRI GAATTC 1 cut(s) 289
EcoRII CCWGG 2 cut(s) 115, 377
EcoT14I CCWWGG 1 cut(s) 148
ErhI CCWWGG 1 cut(s) 148
FaeI CATG 3 cut(s) 191, 212, 530
FaiI YATR 8 cut(s) 15, 20, 72, 189, 210, 507, 509, 528
FatI CATG 3 cut(s) 187, 208, 526
FauNDI CATATG 1 cut(s) 507
FbaI TGATCA 1 cut(s) 313
Fnu4HI GCNGC 6 cut(s) 78, 157, 267, 297, 300, 429
FokI GGATG 1 cut(s) 361
Fsp4HI GCNGC 6 cut(s) 78, 157, 267, 297, 300, 429
FspBI CTAG 1 cut(s) 270
GluI GCNGC 6 cut(s) 78, 157, 267, 297, 300, 429
GsaI CCCAGC 1 cut(s) 110
GsuI CTGGAG 3 cut(s) 116, 138, 330
HaeIII GGCC 1 cut(s) 361
HapII CCGG 1 cut(s) 425
Hin1II CATG 3 cut(s) 191, 212, 530
HindIII AAGCTT 1 cut(s) 412
HinfI GANTC 4 cut(s) 201, 308, 434, 440
HpaII CCGG 1 cut(s) 425
HphI GGTGA 1 cut(s) 435
Hpy188I TCNGA 9 cut(s) 259, 280, 313, 318, 366, 398, 439, 481, 546
Hpy188III TCNNGA 3 cut(s) 188, 209, 305
HpyAV CCTTC 2 cut(s) 454, 525
HpyCH4III ACNGT 1 cut(s) 248
HpyCH4V TGCA 5 cut(s) 77, 159, 266, 299, 392
HpyF10VI GCNNNNNNNGC 2 cut(s) 272, 425
HpyF3I CTNAG 1 cut(s) 101
Hsp92II CATG 3 cut(s) 191, 212, 530
Ksp22I TGATCA 1 cut(s) 313
Kzo9I GATC 7 cut(s) 127, 259, 313, 366, 398, 519, 523
LmnI GCTCC 3 cut(s) 97, 307, 349
Lsp1109I GCAGC 6 cut(s) 89, 143, 278, 283, 311, 440
MaeI CTAG 1 cut(s) 270
MaeIII GTNAC 2 cut(s) 135, 441
MalI GATC 7 cut(s) 129, 261, 315, 368, 400, 521, 525
MboI GATC 7 cut(s) 127, 259, 313, 366, 398, 519, 523
MboII GAAGA 4 cut(s) 50, 95, 137, 236
MflI RGATCY 1 cut(s) 259
MluCI AATT 3 cut(s) 289, 333, 553
MlyI GAGTC 3 cut(s) 210, 302, 449
MnlI CCTC 8 cut(s) 59, 96, 113, 123, 195, 205, 372, 457
MroXI GAANNNNTTC 1 cut(s) 535
MseI TTAA 1 cut(s) 556
MspA1I CMGCKG 2 cut(s) 296, 431
MspI CCGG 1 cut(s) 425
MspR9I CCNGG 3 cut(s) 117, 379, 425
MvaI CCWGG 2 cut(s) 117, 379
MwoI GCNNNNNNNGC 2 cut(s) 272, 425
NciI CCSGG 1 cut(s) 425
NdeI CATATG 1 cut(s) 507
NdeII GATC 7 cut(s) 127, 259, 313, 366, 398, 519, 523
NlaIII CATG 3 cut(s) 191, 212, 530
NlaIV GGNNCC 1 cut(s) 111
NmuCI GTSAC 2 cut(s) 135, 441
NspV TTCGAA 1 cut(s) 458
PagI TCATGA 2 cut(s) 187, 208
PdmI GAANNNNTTC 1 cut(s) 535
PfeI GAWTC 1 cut(s) 434
PfoI TCCNGGA 2 cut(s) 115, 377
PkrI GCNGC 6 cut(s) 79, 158, 268, 298, 301, 430
PleI GAGTC 3 cut(s) 209, 302, 448
PpsI GAGTC 3 cut(s) 209, 302, 448
PpuMI RGGWCCY 1 cut(s) 110
Psp5II RGGWCCY 1 cut(s) 110
Psp6I CCWGG 2 cut(s) 115, 377
PspFI CCCAGC 1 cut(s) 106
PspGI CCWGG 2 cut(s) 115, 377
PspN4I GGNNCC 1 cut(s) 111
PspPI GGNCC 1 cut(s) 110
PspPPI RGGWCCY 1 cut(s) 110
PstI CTGCAG 2 cut(s) 268, 301
PstNI CAGNNNCTG 2 cut(s) 263, 305
PsuI RGATCY 1 cut(s) 259
PvuII CAGCTG 2 cut(s) 296, 431
SaqAI TTAA 1 cut(s) 556
SatI GCNGC 6 cut(s) 78, 157, 267, 297, 300, 429
Sau3AI GATC 7 cut(s) 127, 259, 313, 366, 398, 519, 523
Sau96I GGNCC 1 cut(s) 110
SchI GAGTC 3 cut(s) 210, 302, 449
ScrFI CCNGG 3 cut(s) 117, 379, 425
SetI ASST 7 cut(s) 96, 102, 271, 298, 304, 416, 433
SfcI CTRYAG 2 cut(s) 264, 297
SfuI TTCGAA 1 cut(s) 458
SinI GGWCC 1 cut(s) 110
Sse9I AATT 3 cut(s) 289, 333, 553
SspI AATATT 1 cut(s) 181
SspMI CTAG 1 cut(s) 270
StyD4I CCNGG 3 cut(s) 115, 377, 423
StyI CCWWGG 1 cut(s) 148
TaaI ACNGT 1 cut(s) 248
TaqI TCGA 2 cut(s) 458, 522
TasI AATT 3 cut(s) 289, 333, 553
TfiI GAWTC 1 cut(s) 434
Tru1I TTAA 1 cut(s) 556
Tru9I TTAA 1 cut(s) 556
TseFI GTSAC 2 cut(s) 135, 441
TseI GCWGC 6 cut(s) 77, 156, 266, 296, 299, 428
Tsp45I GTSAC 2 cut(s) 135, 441
TspDTI ATGAA 5 cut(s) 51, 204, 240, 363, 465
TspGWI ACGGA 1 cut(s) 359
VpaK11BI GGWCC 1 cut(s) 110
XagI CCTNNNNNAGG 1 cut(s) 117
XapI RAATTY 1 cut(s) 289
XcmI CCANNNNNNNNNTGG 1 cut(s) 354
XmnI GAANNNNTTC 1 cut(s) 535
XspI CTAG 1 cut(s) 270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.