pycom15g08530

Lachrymatory-factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
N/A
Physical Location & Seq
Forward (+)
5362750 .. 5364760
2011 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 438 bp
ATGGAAGAAAACCAATCACCATTAAAATGGGAAGGCAAAACCGCTTGTGAGCTCATAGGCCATGCAGCAGAACAAGTTTGGCCTCTTCGGGCCGATTTTTGTAGCTTGCACAAATGGTTGCCAATCCTTGACACATGTTACCTAGTTGACGGCATGGATGGAGCTGGCTTAGGTTCAGTGAGGACTCTTTTAGTTGCTTGTGACATTAGTATTAACTCTTTAATGCATTATTGTTATCCAAAGAATATGGAGAATGCTCTTTGGGTTCAAGTCGTATGCATCACGGTCAGGGTTGTTCCGCGAGGGGATATAGACGGTCGAGATGGGTCCGTGACCGTTGATCCTGTGGAAGGGTGGGTTTTTGATGATTTGGTGAAAAAATATGAGGTGGAATGGCAGAAAATGTCCAAGAGAATGGAAGGTGCACCTTGTATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Polyketide_cyc2

Protein Analysis

146

Amino Acids

16.33

Weight (kDa)

4.87

Isoelectric Point (pI)

42.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 301
AciI CCGC 2 cut(s) 42, 299
AclWI GGATC 1 cut(s) 335
AfiI CCNNNNNNNGG 1 cut(s) 350
AflIII ACRYGT 1 cut(s) 134
AgsI TTSAA 1 cut(s) 269
AluBI AGCT 3 cut(s) 52, 105, 164
AluI AGCT 3 cut(s) 52, 105, 164
Alw21I GWGCWC 2 cut(s) 54, 427
Alw44I GTGCAC 1 cut(s) 423
AlwI GGATC 1 cut(s) 335
AoxI GGCC 3 cut(s) 58, 80, 90
ApaLI GTGCAC 1 cut(s) 423
ApeKI GCWGC 1 cut(s) 65
AspS9I GGNCC 2 cut(s) 90, 327
AsuHPI GGTGA 2 cut(s) 9, 385
AvaII GGWCC 1 cut(s) 327
BaeGI GKGCMC 1 cut(s) 427
BanII GRGCYC 1 cut(s) 54
Bbv12I GWGCWC 2 cut(s) 54, 427
BbvI GCAGC 1 cut(s) 77
BccI CCATC 2 cut(s) 152, 317
BceAI ACGGC 1 cut(s) 166
BfaI CTAG 1 cut(s) 143
BisI GCNGC 1 cut(s) 66
BlsI GCNGC 1 cut(s) 67
Bme18I GGWCC 1 cut(s) 327
BmgT120I GGNCC 2 cut(s) 90, 327
BmiI GGNNCC 1 cut(s) 328
BmsI GCATC 1 cut(s) 288
Bpu10I CCTNAGC 1 cut(s) 169
Bsc4I CCNNNNNNNGG 1 cut(s) 350
BseGI GGATG 1 cut(s) 163
BseLI CCNNNNNNNGG 1 cut(s) 350
BseSI GKGCMC 1 cut(s) 427
BseXI GCAGC 1 cut(s) 77
Bsh1236I CGCG 1 cut(s) 301
Bsh1285I CGRYCG 1 cut(s) 319
BshFI GGCC 3 cut(s) 60, 82, 92
BsiEI CGRYCG 1 cut(s) 319
BsiHKAI GWGCWC 2 cut(s) 54, 427
BslI CCNNNNNNNGG 1 cut(s) 350
BsmI GAATGC 1 cut(s) 259
BsnI GGCC 3 cut(s) 60, 82, 92
Bsp1286I GDGCHC 2 cut(s) 54, 427
Bsp143I GATC 1 cut(s) 340
BspACI CCGC 2 cut(s) 42, 299
BspANI GGCC 3 cut(s) 60, 82, 92
BspFNI CGCG 1 cut(s) 301
BspLI GGNNCC 1 cut(s) 328
BspPI GGATC 1 cut(s) 335
BssMI GATC 1 cut(s) 340
Bst4CI ACNGT 3 cut(s) 286, 317, 337
Bst6I CTCTTC 1 cut(s) 90
BstC8I GCNNGC 2 cut(s) 107, 166
BstDEI CTNAG 1 cut(s) 169
BstENI CCTNNNNNAGG 1 cut(s) 348
BstF5I GGATG 1 cut(s) 163
BstFNI CGCG 1 cut(s) 301
BstKTI GATC 1 cut(s) 343
BstMBI GATC 1 cut(s) 340
BstMCI CGRYCG 1 cut(s) 319
BstNSI RCATGY 1 cut(s) 138
BstSLI GKGCMC 1 cut(s) 427
BstUI CGCG 1 cut(s) 301
BstV1I GCAGC 1 cut(s) 77
BstXI CCANNNNNNTGG 2 cut(s) 27, 415
BsuRI GGCC 3 cut(s) 60, 82, 92
BtsCI GGATG 1 cut(s) 163
BtsIMutI CAGTG 1 cut(s) 183
Cac8I GCNNGC 2 cut(s) 107, 166
Cfr13I GGNCC 2 cut(s) 90, 327
CviAII CATG 3 cut(s) 62, 135, 154
CviJI RGCY 7 cut(s) 52, 60, 82, 92, 105, 164, 168
CviKI_1 RGCY 7 cut(s) 52, 60, 82, 92, 105, 164, 168
DdeI CTNAG 1 cut(s) 169
DpnI GATC 1 cut(s) 342
DpnII GATC 1 cut(s) 340
Eam1104I CTCTTC 1 cut(s) 90
EarI CTCTTC 1 cut(s) 90
Ecl136II GAGCTC 1 cut(s) 52
Eco24I GRGCYC 1 cut(s) 54
Eco47I GGWCC 1 cut(s) 327
Eco53kI GAGCTC 1 cut(s) 52
EcoICRI GAGCTC 1 cut(s) 52
EcoNI CCTNNNNNAGG 1 cut(s) 348
EcoT22I ATGCAT 2 cut(s) 228, 281
EcoT38I GRGCYC 1 cut(s) 54
FaeI CATG 3 cut(s) 65, 138, 157
FaiI YATR 8 cut(s) 56, 63, 136, 155, 248, 277, 311, 384
FatI CATG 3 cut(s) 61, 134, 153
Fnu4HI GCNGC 1 cut(s) 66
FokI GGATG 1 cut(s) 170
FriOI GRGCYC 1 cut(s) 54
Fsp4HI GCNGC 1 cut(s) 66
FspBI CTAG 1 cut(s) 143
GluI GCNGC 1 cut(s) 66
HaeIII GGCC 3 cut(s) 60, 82, 92
Hin1II CATG 3 cut(s) 65, 138, 157
HincII GTYRAC 1 cut(s) 148
HindII GTYRAC 1 cut(s) 148
HinfI GANTC 1 cut(s) 184
HphI GGTGA 2 cut(s) 9, 385
Hpy166II GTNNAC 2 cut(s) 148, 425
Hpy188III TCNNGA 1 cut(s) 320
Hpy8I GTNNAC 2 cut(s) 148, 425
HpyAV CCTTC 3 cut(s) 26, 344, 413
HpyCH4III ACNGT 3 cut(s) 286, 317, 337
HpyCH4V TGCA 5 cut(s) 65, 109, 226, 279, 425
HpyF3I CTNAG 1 cut(s) 169
Hsp92II CATG 3 cut(s) 65, 138, 157
Kzo9I GATC 1 cut(s) 340
LmnI GCTCC 1 cut(s) 161
LpnPI CCDG 3 cut(s) 150, 274, 357
Lsp1109I GCAGC 1 cut(s) 77
LweI GCATC 1 cut(s) 288
MaeI CTAG 1 cut(s) 143
MaeIII GTNAC 3 cut(s) 137, 200, 331
MalI GATC 1 cut(s) 342
MboI GATC 1 cut(s) 340
MboII GAAGA 2 cut(s) 17, 77
MhlI GDGCHC 2 cut(s) 54, 427
MlyI GAGTC 1 cut(s) 178
MnlI CCTC 4 cut(s) 93, 174, 296, 379
Mph1103I ATGCAT 2 cut(s) 228, 281
MseI TTAA 3 cut(s) 23, 213, 221
MslI CAYNNNNRTG 1 cut(s) 25
Mva1269I GAATGC 1 cut(s) 259
MvnI CGCG 1 cut(s) 301
NdeII GATC 1 cut(s) 340
NlaIII CATG 3 cut(s) 65, 138, 157
NlaIV GGNNCC 1 cut(s) 328
NmuCI GTSAC 2 cut(s) 200, 331
NsiI ATGCAT 2 cut(s) 228, 281
NspI RCATGY 1 cut(s) 138
PciI ACATGT 1 cut(s) 134
PctI GAATGC 1 cut(s) 259
PkrI GCNGC 1 cut(s) 67
PleI GAGTC 1 cut(s) 178
PpsI GAGTC 1 cut(s) 178
PscI ACATGT 1 cut(s) 134
Psp124BI GAGCTC 1 cut(s) 54
PspN4I GGNNCC 1 cut(s) 328
PspPI GGNCC 2 cut(s) 90, 327
RseI CAYNNNNRTG 1 cut(s) 25
SacI GAGCTC 1 cut(s) 54
SaqAI TTAA 3 cut(s) 23, 213, 221
SatI GCNGC 1 cut(s) 66
Sau3AI GATC 1 cut(s) 340
Sau96I GGNCC 2 cut(s) 90, 327
SchI GAGTC 1 cut(s) 178
SduI GDGCHC 2 cut(s) 54, 427
SetI ASST 8 cut(s) 54, 107, 144, 166, 175, 390, 424, 430
SfaNI GCATC 1 cut(s) 288
SinI GGWCC 1 cut(s) 327
SmiMI CAYNNNNRTG 1 cut(s) 25
SsiI CCGC 2 cut(s) 42, 299
SspMI CTAG 1 cut(s) 143
SstI GAGCTC 1 cut(s) 54
TaaI ACNGT 3 cut(s) 286, 317, 337
TaqI TCGA 1 cut(s) 319
Tru1I TTAA 3 cut(s) 23, 213, 221
Tru9I TTAA 3 cut(s) 23, 213, 221
TscAI CASTG 1 cut(s) 183
TseFI GTSAC 2 cut(s) 200, 331
TseI GCWGC 1 cut(s) 65
Tsp45I GTSAC 2 cut(s) 200, 331
TspGWI ACGGA 1 cut(s) 319
TspRI CASTG 1 cut(s) 183
VneI GTGCAC 1 cut(s) 423
VpaK11BI GGWCC 1 cut(s) 327
XagI CCTNNNNNAGG 1 cut(s) 348
XceI RCATGY 1 cut(s) 138
XspI CTAG 1 cut(s) 143
Zsp2I ATGCAT 2 cut(s) 228, 281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.