pycom15g10110

Ribosomal RNA small subunit methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
N/A
Physical Location & Seq
Forward (+)
6583998 .. 6584773
776 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 450 bp
ATGTGGTCACAATTTGTACAGCAAAAGGAAATGGCAATAGCATCAGCACTATCGTCATCAACCTCAAAGCTCTTCTCTTTCGCCTCACTATCATTCCGCCTGAGAACCCTCACAACCACCGCACGCAGCGCCAGAAGCAACTGCAGCGCTGCCAGCAAGAAGAGGAAGGAGAAGCAGGAGCTGAGGCAGAGAGTGGTGTTAAAGGAGAAGAGGAGAACGCGTTCCGATAGGGAATTCCACTTGGAAACAATTAAGCGTTATGGAGGTACCGGTACCCACATTCCGGCCATGCTCGGCGAAGTTCTGGATGTTTTCCCGGCGTCCAAACAACTCCGGTACTTCGTCGACTGCACTGTCGGAGCTGCCGGGCATTCCTCTGCGATAATTGAAGGGCATCCGGAAATGGAAGTGTATGTTGGGCTTGATGTCGATCGATCCTGTGGCGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_5

Protein Analysis

150

Amino Acids

16.62

Weight (kDa)

9.84

Isoelectric Point (pI)

52.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_5 PF01795 92 - 146 1.2e-07 MraW methylase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 353
Acc65I GGTACC 2 cut(s) 266, 272
AccB1I GGYRCC 2 cut(s) 266, 272
AccI GTMKAC 1 cut(s) 345
AccII CGCG 1 cut(s) 220
AccIII TCCGGA 1 cut(s) 397
AciI CCGC 2 cut(s) 97, 120
AclWI GGATC 1 cut(s) 429
AcoI YGGCCR 1 cut(s) 285
AcsI RAATTY 1 cut(s) 233
AcyI GRCGYC 1 cut(s) 320
AfaI GTAC 4 cut(s) 18, 268, 274, 338
AfeI AGCGCT 1 cut(s) 148
AfiI CCNNNNNNNGG 1 cut(s) 283
AflIII ACRYGT 1 cut(s) 218
AgeI ACCGGT 1 cut(s) 269
AgsI TTSAA 1 cut(s) 389
AjuI GAANNNNNNNTTGG 2 cut(s) 399, 431
AloI GAACNNNNNNTCC 2 cut(s) 205, 237
AluBI AGCT 3 cut(s) 70, 181, 362
AluI AGCT 3 cut(s) 70, 181, 362
AlwI GGATC 1 cut(s) 429
AlwNI CAGNNNCTG 1 cut(s) 181
Aor13HI TCCGGA 1 cut(s) 397
Aor51HI AGCGCT 1 cut(s) 148
AoxI GGCC 1 cut(s) 285
ApeKI GCWGC 4 cut(s) 126, 144, 149, 362
ApoI RAATTY 1 cut(s) 233
AsiGI ACCGGT 1 cut(s) 269
Asp700I GAANNNNTTC 1 cut(s) 220
Asp718I GGTACC 2 cut(s) 266, 272
AspLEI GCGC 3 cut(s) 131, 149, 446
AsuC2I CCSGG 2 cut(s) 317, 367
BanI GGYRCC 2 cut(s) 266, 272
BbvCI CCTCAGC 1 cut(s) 182
BbvI GCAGC 4 cut(s) 136, 138, 156, 349
BcnI CCSGG 2 cut(s) 317, 367
BfmI CTRYAG 1 cut(s) 142
BfoI RGCGCY 3 cut(s) 132, 150, 447
BisI GCNGC 4 cut(s) 127, 145, 150, 363
BlsI GCNGC 4 cut(s) 128, 146, 151, 364
Bme1390I CCNGG 2 cut(s) 317, 367
BmiI GGNNCC 2 cut(s) 268, 274
BmrFI CCNGG 2 cut(s) 317, 367
BmsI GCATC 2 cut(s) 50, 403
Bpu10I CCTNAGC 1 cut(s) 182
BpuMI CCSGG 2 cut(s) 317, 367
Bsa29I ATCGAT 1 cut(s) 433
BsaBI GATNNNNATC 1 cut(s) 429
BsaHI GRCGYC 1 cut(s) 320
BsaWI WCCGGW 3 cut(s) 269, 333, 397
BsaXI ACNNNNNCTCC 2 cut(s) 205, 235
Bsc4I CCNNNNNNNGG 1 cut(s) 283
Bse118I RCCGGY 1 cut(s) 269
Bse8I GATNNNNATC 1 cut(s) 429
BseAI TCCGGA 1 cut(s) 397
BseCI ATCGAT 1 cut(s) 433
BseGI GGATG 2 cut(s) 313, 394
BseJI GATNNNNATC 1 cut(s) 429
BseLI CCNNNNNNNGG 1 cut(s) 283
BseMII CTCAG 2 cut(s) 92, 173
BseRI GAGGAG 1 cut(s) 226
BseXI GCAGC 4 cut(s) 136, 138, 156, 349
BsgI GTGCAG 1 cut(s) 334
Bsh1236I CGCG 1 cut(s) 220
Bsh1285I CGRYCG 1 cut(s) 433
BshFI GGCC 1 cut(s) 287
BshNI GGYRCC 2 cut(s) 266, 272
BshTI ACCGGT 1 cut(s) 269
BshVI ATCGAT 1 cut(s) 433
BsiEI CGRYCG 1 cut(s) 433
BsiSI CCGG 6 cut(s) 270, 284, 317, 334, 366, 398
BslI CCNNNNNNNGG 1 cut(s) 283
BsmI GAATGC 1 cut(s) 370
BsnI GGCC 1 cut(s) 287
Bsp13I TCCGGA 1 cut(s) 397
Bsp1407I TGTACA 1 cut(s) 16
Bsp143I GATC 2 cut(s) 430, 434
BspACI CCGC 2 cut(s) 97, 120
BspANI GGCC 1 cut(s) 287
BspCNI CTCAG 2 cut(s) 93, 174
BspDI ATCGAT 1 cut(s) 433
BspEI TCCGGA 1 cut(s) 397
BspFNI CGCG 1 cut(s) 220
BspLI GGNNCC 2 cut(s) 268, 274
BspMAI CTGCAG 1 cut(s) 146
BspPI GGATC 1 cut(s) 429
BspQI GCTCTTC 1 cut(s) 77
BspT107I GGYRCC 2 cut(s) 266, 272
BsrFI RCCGGY 1 cut(s) 269
BsrGI TGTACA 1 cut(s) 16
BssAI RCCGGY 1 cut(s) 269
BssMI GATC 2 cut(s) 430, 434
BssNI GRCGYC 1 cut(s) 320
Bst4CI ACNGT 1 cut(s) 355
Bst6I CTCTTC 3 cut(s) 77, 155, 203
BstACI GRCGYC 1 cut(s) 320
BstAUI TGTACA 1 cut(s) 16
BstC8I GCNNGC 2 cut(s) 124, 154
BstDEI CTNAG 2 cut(s) 101, 182
BstF5I GGATG 2 cut(s) 313, 394
BstFNI CGCG 1 cut(s) 220
BstH2I RGCGCY 3 cut(s) 132, 150, 447
BstHHI GCGC 3 cut(s) 131, 149, 446
BstKTI GATC 2 cut(s) 433, 437
BstMBI GATC 2 cut(s) 430, 434
BstMCI CGRYCG 1 cut(s) 433
BstMWI GCNNNNNNNGC 4 cut(s) 128, 135, 144, 153
BstSCI CCNGG 2 cut(s) 315, 365
BstSFI CTRYAG 1 cut(s) 142
BstUI CGCG 1 cut(s) 220
BstV1I GCAGC 4 cut(s) 136, 138, 156, 349
Bsu15I ATCGAT 1 cut(s) 433
BsuRI GGCC 1 cut(s) 287
BsuTUI ATCGAT 1 cut(s) 433
BtsCI GGATG 2 cut(s) 313, 394
BtsIMutI CAGTG 1 cut(s) 351
Cac8I GCNNGC 2 cut(s) 124, 154
CaiI CAGNNNCTG 1 cut(s) 181
CfoI GCGC 3 cut(s) 131, 149, 446
Cfr10I RCCGGY 1 cut(s) 269
ClaI ATCGAT 1 cut(s) 433
CseI GACGC 1 cut(s) 309
Csp6I GTAC 4 cut(s) 17, 267, 273, 337
CspAI ACCGGT 1 cut(s) 269
CviAII CATG 1 cut(s) 289
CviJI RGCY 5 cut(s) 70, 181, 287, 362, 421
CviKI_1 RGCY 5 cut(s) 70, 181, 287, 362, 421
CviQI GTAC 4 cut(s) 17, 267, 273, 337
DdeI CTNAG 2 cut(s) 101, 182
DpnI GATC 2 cut(s) 432, 436
DpnII GATC 2 cut(s) 430, 434
DrdI GACNNNNNNGTC 1 cut(s) 353
DseDI GACNNNNNNGTC 1 cut(s) 353
EaeI YGGCCR 1 cut(s) 285
Eam1104I CTCTTC 3 cut(s) 77, 155, 203
EarI CTCTTC 3 cut(s) 77, 155, 203
EciI GGCGGA 1 cut(s) 86
Eco47III AGCGCT 1 cut(s) 148
EcoRI GAATTC 1 cut(s) 233
FaeI CATG 1 cut(s) 292
FaiI YATR 3 cut(s) 261, 290, 414
FatI CATG 1 cut(s) 288
FblI GTMKAC 1 cut(s) 345
Fnu4HI GCNGC 4 cut(s) 127, 145, 150, 363
FokI GGATG 2 cut(s) 320, 381
Fsp4HI GCNGC 4 cut(s) 127, 145, 150, 363
GlaI GCGC 3 cut(s) 130, 148, 445
GluI GCNGC 4 cut(s) 127, 145, 150, 363
HaeII RGCGCY 3 cut(s) 132, 150, 447
HaeIII GGCC 1 cut(s) 287
HapII CCGG 6 cut(s) 270, 284, 317, 334, 366, 398
HgaI GACGC 1 cut(s) 309
HhaI GCGC 3 cut(s) 131, 149, 446
Hin1I GRCGYC 1 cut(s) 320
Hin1II CATG 1 cut(s) 292
Hin6I GCGC 3 cut(s) 129, 147, 444
HinP1I GCGC 3 cut(s) 129, 147, 444
HincII GTYRAC 1 cut(s) 346
HindII GTYRAC 1 cut(s) 346
HpaII CCGG 6 cut(s) 270, 284, 317, 334, 366, 398
Hpy166II GTNNAC 1 cut(s) 346
Hpy188I TCNGA 2 cut(s) 226, 359
Hpy188III TCNNGA 2 cut(s) 305, 398
Hpy8I GTNNAC 1 cut(s) 346
Hpy99I CGWCG 1 cut(s) 347
HpyAV CCTTC 2 cut(s) 160, 383
HpyCH4III ACNGT 1 cut(s) 355
HpyCH4V TGCA 2 cut(s) 144, 351
HpyF10VI GCNNNNNNNGC 4 cut(s) 128, 135, 144, 153
HpyF3I CTNAG 2 cut(s) 101, 182
Hsp92I GRCGYC 1 cut(s) 320
Hsp92II CATG 1 cut(s) 292
HspAI GCGC 3 cut(s) 129, 147, 444
Kpn2I TCCGGA 1 cut(s) 397
KpnI GGTACC 2 cut(s) 270, 276
Kzo9I GATC 2 cut(s) 430, 434
LguI GCTCTTC 1 cut(s) 77
LmnI GCTCC 2 cut(s) 178, 359
Lsp1109I GCAGC 4 cut(s) 136, 138, 156, 349
LweI GCATC 2 cut(s) 50, 403
MaeIII GTNAC 1 cut(s) 6
MalI GATC 2 cut(s) 432, 436
MboI GATC 2 cut(s) 430, 434
MboII GAAGA 3 cut(s) 64, 172, 220
MluCI AATT 4 cut(s) 11, 233, 249, 384
MluI ACGCGT 1 cut(s) 218
MmeI TCCRAC 1 cut(s) 337
MnlI CCTC 8 cut(s) 73, 94, 119, 156, 177, 204, 257, 385
MroI TCCGGA 1 cut(s) 397
MroXI GAANNNNTTC 1 cut(s) 220
MseI TTAA 2 cut(s) 200, 252
MspI CCGG 6 cut(s) 270, 284, 317, 334, 366, 398
MspR9I CCNGG 2 cut(s) 317, 367
Mva1269I GAATGC 1 cut(s) 370
MvnI CGCG 1 cut(s) 220
MwoI GCNNNNNNNGC 4 cut(s) 128, 135, 144, 153
NciI CCSGG 2 cut(s) 317, 367
NdeII GATC 2 cut(s) 430, 434
NlaIII CATG 1 cut(s) 292
NlaIV GGNNCC 2 cut(s) 268, 274
NmeAIII GCCGAG 1 cut(s) 273
NmuCI GTSAC 1 cut(s) 6
PciSI GCTCTTC 1 cut(s) 77
PctI GAATGC 1 cut(s) 370
PdmI GAANNNNTTC 1 cut(s) 220
PinAI ACCGGT 1 cut(s) 269
PkrI GCNGC 4 cut(s) 128, 146, 151, 364
Ple19I CGATCG 1 cut(s) 433
PspN4I GGNNCC 2 cut(s) 268, 274
PstI CTGCAG 1 cut(s) 146
PstNI CAGNNNCTG 1 cut(s) 181
PvuI CGATCG 1 cut(s) 433
RsaI GTAC 4 cut(s) 18, 268, 274, 338
RsaNI GTAC 4 cut(s) 17, 267, 273, 337
SalI GTCGAC 1 cut(s) 344
SapI GCTCTTC 1 cut(s) 77
SaqAI TTAA 2 cut(s) 200, 252
SatI GCNGC 4 cut(s) 127, 145, 150, 363
Sau3AI GATC 2 cut(s) 430, 434
ScrFI CCNGG 2 cut(s) 317, 367
SetI ASST 5 cut(s) 65, 72, 183, 268, 364
SfaNI GCATC 2 cut(s) 50, 403
SfcI CTRYAG 1 cut(s) 142
Sse9I AATT 4 cut(s) 11, 233, 249, 384
SsiI CCGC 2 cut(s) 97, 120
StyD4I CCNGG 2 cut(s) 315, 365
TaaI ACNGT 1 cut(s) 355
TaqI TCGA 3 cut(s) 345, 429, 433
TasI AATT 4 cut(s) 11, 233, 249, 384
TatI WGTACW 1 cut(s) 16
Tru1I TTAA 2 cut(s) 200, 252
Tru9I TTAA 2 cut(s) 200, 252
TscAI CASTG 1 cut(s) 358
TseFI GTSAC 1 cut(s) 6
TseI GCWGC 4 cut(s) 126, 144, 149, 362
Tsp45I GTSAC 1 cut(s) 6
TspRI CASTG 1 cut(s) 358
XapI RAATTY 1 cut(s) 233
XmiI GTMKAC 1 cut(s) 345
XmnI GAANNNNTTC 1 cut(s) 220
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.