pycom15g16930

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
N/A
Physical Location & Seq
Forward (+)
11899147 .. 11899575
429 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 429 bp
ATGGCGAAATCTCTCTTCGTCTGCTTCTCTCTCCTCCTCCTCCTCCCCCTCACCTCCCAAACCACTGACCCGAACACCTCGAACCGGAACTCAATTTCAAGCCCATCTCCGGCTCACACCGAGCTCACCAACTACGGCTTCCCAATCGGTCTCCTCCCCACCGCCGTCAAGAACTACACCATCAACCGCACCACCGGCGACTTCATCGTTGACCTCGGCGGCGCGTGCAAGGTAACCCTCCCACCCGACAATTACCTGGCTACCTACTCGAAGAAAATCACTGGAAAGATCGATCGAGGGAAAAATCGCCGAGATCAACGGCATCAGCGTCCGGGCCTTCTTCCAGTGGTGGTCGATCACCGGAATCCGGTCCAGCGGCGACAACTTGGTGTTCGAGGTCGGCATGGTCTCCGCCAAGTACCCCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

143

Amino Acids

15.76

Weight (kDa)

10.56

Isoelectric Point (pI)

41.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 39 - 101 1.2e-13 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 224
AciI CCGC 5 cut(s) 162, 187, 219, 376, 412
AfaI GTAC 1 cut(s) 420
AfiI CCNNNNNNNGG 3 cut(s) 84, 109, 367
AgsI TTSAA 1 cut(s) 99
AjnI CCWGG 1 cut(s) 255
AjuI GAANNNNNNNTTGG 2 cut(s) 122, 154
AluBI AGCT 1 cut(s) 124
AluI AGCT 1 cut(s) 124
Alw21I GWGCWC 1 cut(s) 126
Alw26I GTCTC 2 cut(s) 155, 413
AoxI GGCC 1 cut(s) 334
AspLEI GCGC 1 cut(s) 224
AspS9I GGNCC 2 cut(s) 334, 370
AsuC2I CCSGG 1 cut(s) 333
AsuHPI GGTGA 3 cut(s) 43, 118, 350
AvaII GGWCC 1 cut(s) 370
BanII GRGCYC 1 cut(s) 126
Bbv12I GWGCWC 1 cut(s) 126
BccI CCATC 2 cut(s) 112, 188
BceAI ACGGC 3 cut(s) 149, 151, 335
BciT130I CCWGG 1 cut(s) 257
BcnI CCSGG 1 cut(s) 333
BcoDI GTCTC 2 cut(s) 155, 413
BisI GCNGC 2 cut(s) 220, 377
BlsI GCNGC 2 cut(s) 221, 378
Bme1390I CCNGG 2 cut(s) 257, 333
Bme18I GGWCC 1 cut(s) 370
BmgT120I GGNCC 2 cut(s) 334, 370
BmrFI CCNGG 2 cut(s) 257, 333
BmsI GCATC 1 cut(s) 331
BpuMI CCSGG 1 cut(s) 333
Bsa29I ATCGAT 1 cut(s) 291
BsaI GGTCTC 2 cut(s) 155, 413
BsaJI CCNNGG 1 cut(s) 214
BsaWI WCCGGW 3 cut(s) 84, 360, 367
Bsc4I CCNNNNNNNGG 3 cut(s) 84, 109, 367
Bse118I RCCGGY 1 cut(s) 194
Bse1I ACTGG 2 cut(s) 286, 344
BseBI CCWGG 1 cut(s) 257
BseCI ATCGAT 1 cut(s) 291
BseDI CCNNGG 1 cut(s) 214
BseLI CCNNNNNNNGG 3 cut(s) 84, 109, 367
BseNI ACTGG 2 cut(s) 286, 344
BseRI GAGGAG 5 cut(s) 23, 26, 29, 32, 143
Bsh1236I CGCG 1 cut(s) 224
Bsh1285I CGRYCG 1 cut(s) 295
BshFI GGCC 1 cut(s) 336
BshVI ATCGAT 1 cut(s) 291
BsiEI CGRYCG 1 cut(s) 295
BsiHKAI GWGCWC 1 cut(s) 126
BsiSI CCGG 6 cut(s) 85, 110, 195, 332, 361, 368
BslI CCNNNNNNNGG 3 cut(s) 84, 109, 367
BsmAI GTCTC 2 cut(s) 155, 413
BsnI GGCC 1 cut(s) 336
Bso31I GGTCTC 2 cut(s) 155, 413
Bsp1286I GDGCHC 1 cut(s) 126
Bsp143I GATC 4 cut(s) 288, 292, 313, 355
BspACI CCGC 5 cut(s) 162, 187, 219, 376, 412
BspANI GGCC 1 cut(s) 336
BspDI ATCGAT 1 cut(s) 291
BspFNI CGCG 1 cut(s) 224
BspTNI GGTCTC 2 cut(s) 155, 413
BsrFI RCCGGY 1 cut(s) 194
BsrI ACTGG 2 cut(s) 286, 344
BssAI RCCGGY 1 cut(s) 194
BssECI CCNNGG 1 cut(s) 214
BssMI GATC 4 cut(s) 288, 292, 313, 355
Bst2UI CCWGG 1 cut(s) 257
Bst6I CTCTTC 1 cut(s) 20
BstC8I GCNNGC 1 cut(s) 226
BstEII GGTNACC 1 cut(s) 232
BstFNI CGCG 1 cut(s) 224
BstHHI GCGC 1 cut(s) 224
BstKTI GATC 4 cut(s) 291, 295, 316, 358
BstMAI GTCTC 2 cut(s) 155, 413
BstMBI GATC 4 cut(s) 288, 292, 313, 355
BstMCI CGRYCG 1 cut(s) 295
BstMWI GCNNNNNNNGC 2 cut(s) 195, 225
BstNI CCWGG 1 cut(s) 257
BstPI GGTNACC 1 cut(s) 232
BstSCI CCNGG 2 cut(s) 255, 331
BstUI CGCG 1 cut(s) 224
Bsu15I ATCGAT 1 cut(s) 291
BsuRI GGCC 1 cut(s) 336
BsuTUI ATCGAT 1 cut(s) 291
BtsIMutI CAGTG 3 cut(s) 63, 279, 351
Cac8I GCNNGC 1 cut(s) 226
CfoI GCGC 1 cut(s) 224
Cfr10I RCCGGY 1 cut(s) 194
Cfr13I GGNCC 2 cut(s) 334, 370
ClaI ATCGAT 1 cut(s) 291
CseI GACGC 1 cut(s) 317
Csp6I GTAC 1 cut(s) 419
CviAII CATG 1 cut(s) 404
CviJI RGCY 6 cut(s) 102, 113, 124, 138, 260, 336
CviKI_1 RGCY 6 cut(s) 102, 113, 124, 138, 260, 336
CviQI GTAC 1 cut(s) 419
DpnI GATC 4 cut(s) 290, 294, 315, 357
DpnII GATC 4 cut(s) 288, 292, 313, 355
Eam1104I CTCTTC 1 cut(s) 20
EarI CTCTTC 1 cut(s) 20
EciI GGCGGA 1 cut(s) 401
Ecl136II GAGCTC 1 cut(s) 124
Eco24I GRGCYC 1 cut(s) 126
Eco31I GGTCTC 2 cut(s) 155, 413
Eco47I GGWCC 1 cut(s) 370
Eco53kI GAGCTC 1 cut(s) 124
Eco91I GGTNACC 1 cut(s) 232
EcoICRI GAGCTC 1 cut(s) 124
EcoO65I GGTNACC 1 cut(s) 232
EcoRII CCWGG 1 cut(s) 255
EcoT38I GRGCYC 1 cut(s) 126
FaeI CATG 1 cut(s) 407
FaiI YATR 1 cut(s) 405
FatI CATG 1 cut(s) 403
Fnu4HI GCNGC 2 cut(s) 220, 377
FriOI GRGCYC 1 cut(s) 126
Fsp4HI GCNGC 2 cut(s) 220, 377
GlaI GCGC 1 cut(s) 223
GluI GCNGC 2 cut(s) 220, 377
HaeIII GGCC 1 cut(s) 336
HapII CCGG 6 cut(s) 85, 110, 195, 332, 361, 368
HgaI GACGC 1 cut(s) 317
HhaI GCGC 1 cut(s) 224
Hin1II CATG 1 cut(s) 407
Hin6I GCGC 1 cut(s) 222
HinP1I GCGC 1 cut(s) 222
HincII GTYRAC 1 cut(s) 211
HindII GTYRAC 1 cut(s) 211
HinfI GANTC 1 cut(s) 364
HpaII CCGG 6 cut(s) 85, 110, 195, 332, 361, 368
HphI GGTGA 3 cut(s) 43, 118, 350
Hpy166II GTNNAC 1 cut(s) 211
Hpy188III TCNNGA 1 cut(s) 169
Hpy8I GTNNAC 1 cut(s) 211
HpyAV CCTTC 1 cut(s) 347
HpyCH4V TGCA 1 cut(s) 228
HpyF10VI GCNNNNNNNGC 2 cut(s) 195, 225
Hsp92II CATG 1 cut(s) 407
HspAI GCGC 1 cut(s) 222
Kzo9I GATC 4 cut(s) 288, 292, 313, 355
LweI GCATC 1 cut(s) 331
MaeIII GTNAC 1 cut(s) 232
MalI GATC 4 cut(s) 290, 294, 315, 357
MboI GATC 4 cut(s) 288, 292, 313, 355
MboII GAAGA 3 cut(s) 7, 283, 332
MhlI GDGCHC 1 cut(s) 126
MluCI AATT 2 cut(s) 93, 250
MspA1I CMGCKG 1 cut(s) 376
MspI CCGG 6 cut(s) 85, 110, 195, 332, 361, 368
MspR9I CCNGG 2 cut(s) 257, 333
MvaI CCWGG 1 cut(s) 257
MvnI CGCG 1 cut(s) 224
MwoI GCNNNNNNNGC 2 cut(s) 195, 225
NciI CCSGG 1 cut(s) 333
NdeII GATC 4 cut(s) 288, 292, 313, 355
NlaIII CATG 1 cut(s) 407
NmeAIII GCCGAG 2 cut(s) 195, 335
PcsI WCGNNNNNNNCGW 1 cut(s) 325
PfeI GAWTC 1 cut(s) 364
PkrI GCNGC 2 cut(s) 221, 378
Ple19I CGATCG 1 cut(s) 295
Psp124BI GAGCTC 1 cut(s) 126
Psp6I CCWGG 1 cut(s) 255
PspEI GGTNACC 1 cut(s) 232
PspGI CCWGG 1 cut(s) 255
PspPI GGNCC 2 cut(s) 334, 370
PvuI CGATCG 1 cut(s) 295
RsaI GTAC 1 cut(s) 420
RsaNI GTAC 1 cut(s) 419
SacI GAGCTC 1 cut(s) 126
SatI GCNGC 2 cut(s) 220, 377
Sau3AI GATC 4 cut(s) 288, 292, 313, 355
Sau96I GGNCC 2 cut(s) 334, 370
ScrFI CCNGG 2 cut(s) 257, 333
SduI GDGCHC 1 cut(s) 126
SetI ASST 8 cut(s) 56, 80, 126, 216, 234, 258, 266, 400
SfaNI GCATC 1 cut(s) 331
SgrAI CRCCGGYG 1 cut(s) 194
SinI GGWCC 1 cut(s) 370
Sse9I AATT 2 cut(s) 93, 250
SsiI CCGC 5 cut(s) 162, 187, 219, 376, 412
SstI GAGCTC 1 cut(s) 126
StyD4I CCNGG 2 cut(s) 255, 331
TaqI TCGA 6 cut(s) 80, 269, 291, 295, 354, 394
TaqII GACCGA 1 cut(s) 137
TasI AATT 2 cut(s) 93, 250
TauI GCSGC 2 cut(s) 222, 379
TfiI GAWTC 1 cut(s) 364
TscAI CASTG 3 cut(s) 70, 286, 351
TspDTI ATGAA 1 cut(s) 193
TspRI CASTG 3 cut(s) 70, 286, 351
VpaK11BI GGWCC 1 cut(s) 370
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.