pycom15g21960

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
N/A
Physical Location & Seq
Forward (+)
15981599 .. 15982738
1140 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 270 bp
ATGGATACGAACAACGGCGGCAAAGACGAGCTCCCGGACCAATCAAAAGCCTCCAAAAAAGGCAGATCATGCAAAGGAACACTCTATTATTCCTCCGCCCTCAAATCTAAGGCTTACAATCCTCTCTGCGTCGGCATTCCTCGCGCCATCCCCGAAGGTGTTTTTTTCATGGAGGGCTTGAAGCTCCTCTGCCATAGAATTTCTCCACTCACTGTGAAGATTGGCTTCTTGAAAACTTTGAGGTTCTCGAGCTTCTTTAATGGCACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

90

Amino Acids

9.78

Weight (kDa)

9.72

Isoelectric Point (pI)

23.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8204 PF26631 20 - 52 3.4e-12 Domain of unknown function (DUF8204)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 144
AciI CCGC 2 cut(s) 18, 96
AcsI RAATTY 1 cut(s) 198
AgsI TTSAA 2 cut(s) 181, 232
AluBI AGCT 3 cut(s) 31, 184, 252
AluI AGCT 3 cut(s) 31, 184, 252
Alw21I GWGCWC 1 cut(s) 33
Ama87I CYCGRG 1 cut(s) 247
ApoI RAATTY 1 cut(s) 198
AspLEI GCGC 1 cut(s) 146
AspS9I GGNCC 1 cut(s) 37
AsuC2I CCSGG 1 cut(s) 35
AvaI CYCGRG 1 cut(s) 247
AvaII GGWCC 1 cut(s) 37
BanII GRGCYC 1 cut(s) 33
Bbv12I GWGCWC 1 cut(s) 33
BccI CCATC 1 cut(s) 155
BceAI ACGGC 1 cut(s) 31
BcnI CCSGG 1 cut(s) 35
BisI GCNGC 1 cut(s) 19
BlsI GCNGC 1 cut(s) 20
Bme1390I CCNGG 1 cut(s) 35
Bme18I GGWCC 1 cut(s) 37
BmeT110I CYCGRG 1 cut(s) 247
BmgT120I GGNCC 1 cut(s) 37
BmrFI CCNGG 1 cut(s) 35
BpuMI CCSGG 1 cut(s) 35
BseGI GGATG 1 cut(s) 147
BseRI GAGGAG 1 cut(s) 176
Bsh1236I CGCG 1 cut(s) 144
BsiHKAI GWGCWC 1 cut(s) 33
BsiHKCI CYCGRG 1 cut(s) 247
BsiSI CCGG 1 cut(s) 35
BsmI GAATGC 1 cut(s) 135
BsoBI CYCGRG 1 cut(s) 247
Bsp1286I GDGCHC 1 cut(s) 33
Bsp143I GATC 1 cut(s) 65
BspACI CCGC 2 cut(s) 18, 96
BspFNI CGCG 1 cut(s) 144
BssMI GATC 1 cut(s) 65
Bst4CI ACNGT 1 cut(s) 214
BstAPI GCANNNNNTGC 1 cut(s) 69
BstDEI CTNAG 2 cut(s) 108, 267
BstF5I GGATG 1 cut(s) 147
BstFNI CGCG 1 cut(s) 144
BstHHI GCGC 1 cut(s) 146
BstKTI GATC 1 cut(s) 68
BstMBI GATC 1 cut(s) 65
BstMWI GCNNNNNNNGC 2 cut(s) 69, 141
BstSCI CCNGG 1 cut(s) 33
BstUI CGCG 1 cut(s) 144
BtsCI GGATG 1 cut(s) 147
BtsIMutI CAGTG 1 cut(s) 210
CfoI GCGC 1 cut(s) 146
Cfr13I GGNCC 1 cut(s) 37
CseI GACGC 1 cut(s) 118
CviAII CATG 2 cut(s) 69, 169
CviJI RGCY 7 cut(s) 31, 50, 113, 177, 184, 225, 252
CviKI_1 RGCY 7 cut(s) 31, 50, 113, 177, 184, 225, 252
DdeI CTNAG 2 cut(s) 108, 267
DpnI GATC 1 cut(s) 67
DpnII GATC 1 cut(s) 65
EciI GGCGGA 1 cut(s) 85
Ecl136II GAGCTC 1 cut(s) 31
Eco24I GRGCYC 1 cut(s) 33
Eco47I GGWCC 1 cut(s) 37
Eco53kI GAGCTC 1 cut(s) 31
Eco88I CYCGRG 1 cut(s) 247
EcoICRI GAGCTC 1 cut(s) 31
EcoT38I GRGCYC 1 cut(s) 33
FaeI CATG 2 cut(s) 72, 172
FaiI YATR 3 cut(s) 70, 170, 195
FalI AAGNNNNNCTT 2 cut(s) 209, 241
FatI CATG 2 cut(s) 68, 168
Fnu4HI GCNGC 1 cut(s) 19
FokI GGATG 1 cut(s) 134
FriOI GRGCYC 1 cut(s) 33
Fsp4HI GCNGC 1 cut(s) 19
GlaI GCGC 1 cut(s) 145
GluI GCNGC 1 cut(s) 19
HapII CCGG 1 cut(s) 35
HgaI GACGC 1 cut(s) 118
HhaI GCGC 1 cut(s) 146
Hin1II CATG 2 cut(s) 72, 172
Hin6I GCGC 1 cut(s) 144
HinP1I GCGC 1 cut(s) 144
HpaII CCGG 1 cut(s) 35
Hpy188III TCNNGA 2 cut(s) 229, 247
Hpy99I CGWCG 1 cut(s) 134
HpyAV CCTTC 1 cut(s) 149
HpyCH4III ACNGT 1 cut(s) 214
HpyCH4V TGCA 1 cut(s) 72
HpyF10VI GCNNNNNNNGC 2 cut(s) 69, 141
HpyF3I CTNAG 2 cut(s) 108, 267
Hsp92II CATG 2 cut(s) 72, 172
HspAI GCGC 1 cut(s) 144
Kzo9I GATC 1 cut(s) 65
LmnI GCTCC 2 cut(s) 36, 189
LpnPI CCDG 1 cut(s) 48
MalI GATC 1 cut(s) 67
MboI GATC 1 cut(s) 65
MboII GAAGA 1 cut(s) 229
MhlI GDGCHC 1 cut(s) 33
MluCI AATT 1 cut(s) 198
MnlI CCTC 8 cut(s) 61, 103, 110, 132, 150, 166, 197, 234
MseI TTAA 1 cut(s) 258
MspI CCGG 1 cut(s) 35
MspR9I CCNGG 1 cut(s) 35
Mva1269I GAATGC 1 cut(s) 135
MvnI CGCG 1 cut(s) 144
MwoI GCNNNNNNNGC 2 cut(s) 69, 141
NciI CCSGG 1 cut(s) 35
NdeII GATC 1 cut(s) 65
NlaIII CATG 2 cut(s) 72, 172
PaeR7I CTCGAG 1 cut(s) 247
PctI GAATGC 1 cut(s) 135
PfoI TCCNGGA 1 cut(s) 33
PkrI GCNGC 1 cut(s) 20
Psp124BI GAGCTC 1 cut(s) 33
PspPI GGNCC 1 cut(s) 37
SacI GAGCTC 1 cut(s) 33
SaqAI TTAA 1 cut(s) 258
SatI GCNGC 1 cut(s) 19
Sau3AI GATC 1 cut(s) 65
Sau96I GGNCC 1 cut(s) 37
ScrFI CCNGG 1 cut(s) 35
SduI GDGCHC 1 cut(s) 33
SetI ASST 5 cut(s) 33, 160, 186, 245, 254
Sfr274I CTCGAG 1 cut(s) 247
SinI GGWCC 1 cut(s) 37
SlaI CTCGAG 1 cut(s) 247
SmlI CTYRAG 1 cut(s) 247
SmoI CTYRAG 1 cut(s) 247
Sse9I AATT 1 cut(s) 198
SsiI CCGC 2 cut(s) 18, 96
SstI GAGCTC 1 cut(s) 33
StyD4I CCNGG 1 cut(s) 33
TaaI ACNGT 1 cut(s) 214
TaqI TCGA 1 cut(s) 248
TasI AATT 1 cut(s) 198
TauI GCSGC 1 cut(s) 21
Tru1I TTAA 1 cut(s) 258
Tru9I TTAA 1 cut(s) 258
TscAI CASTG 1 cut(s) 217
TspDTI ATGAA 1 cut(s) 157
TspRI CASTG 1 cut(s) 217
VpaK11BI GGWCC 1 cut(s) 37
XapI RAATTY 1 cut(s) 198
XhoI CTCGAG 1 cut(s) 247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.