pycom15g22570

Replication factor C subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
N/A
Physical Location & Seq
Reverse (-)
16684930 .. 16685811
882 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 564 bp
ATGCAAGGCACACAAGTGAGAACATATGGCAATACAATTGTTGGGAATTTTGAGTTTATTGTAAAGTCAACTTACAAAGAGCTTTGCCCATGCAGCTATCCTTTTGAAGATGAACAACCAATTCCACTTGGATGGGAAGAGGTGCTGCTTGAACTCGCTTCAGAAATCCTAGCCGATCCGTCACCCAAGAGATTATTTTTCATCCGGGGAAAGTTTCAAAAGCTTCTTATAGATTTTGTCCATCCGAAACTGATTCTACTGTCACCAAAGTATAATTTTGTGATGGAGTTCGCATTTTCCGAAATGCAGAAGCTCGTGGAACAGTTCCTAAAGGGAGTAGCATCTAGTTCTAAGAGGGAACTCTATTACTGGCATGCTTACTACGAGAAAAGACTCCCCGCAGGGGCGAGTGCATTACTGAAACTAGAAGGTAACATATGCAAACCACTAAAATTCTGCATCCAATTATATAAGCATTCAATAATTTGGCACAAATCCACTTCTGTAGTAAGCTTACAGGTTAATCATGCACTTTTTATGCAGAATTTGTGGCCAAGTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 map03030 map03420 map03430
Pfam Domains
Protein Families
DNA_pol3_delta2 NmrA

Protein Analysis

188

Amino Acids

21.7

Weight (kDa)

8.8

Isoelectric Point (pI)

35.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 399
AclWI GGATC 1 cut(s) 170
AcoI YGGCCR 1 cut(s) 551
AcsI RAATTY 3 cut(s) 46, 452, 544
AcuI CTGAAG 1 cut(s) 144
AfiI CCNNNNNNNGG 1 cut(s) 403
AgsI TTSAA 4 cut(s) 107, 152, 218, 480
AleI CACNNNNGTG 1 cut(s) 14
AluBI AGCT 5 cut(s) 82, 96, 223, 313, 513
AluI AGCT 5 cut(s) 82, 96, 223, 313, 513
AlwI GGATC 1 cut(s) 170
AoxI GGCC 1 cut(s) 551
ApeKI GCWGC 2 cut(s) 93, 145
ApoI RAATTY 3 cut(s) 46, 452, 544
AsuC2I CCSGG 1 cut(s) 206
AsuHPI GGTGA 2 cut(s) 174, 255
BalI TGGCCA 1 cut(s) 553
BauI CACGAG 1 cut(s) 314
BbvI GCAGC 2 cut(s) 105, 132
BccI CCATC 3 cut(s) 126, 249, 277
BcnI CCSGG 1 cut(s) 206
BfaI CTAG 3 cut(s) 170, 345, 425
BfmI CTRYAG 1 cut(s) 504
BisI GCNGC 2 cut(s) 94, 146
BlsI GCNGC 2 cut(s) 95, 147
Bme1390I CCNGG 1 cut(s) 206
BmrFI CCNGG 1 cut(s) 206
BmsI GCATC 2 cut(s) 350, 468
BpuMI CCSGG 1 cut(s) 206
BsaJI CCNNGG 1 cut(s) 205
Bsc4I CCNNNNNNNGG 1 cut(s) 403
Bse1I ACTGG 1 cut(s) 374
BseDI CCNNGG 1 cut(s) 205
BseGI GGATG 4 cut(s) 137, 201, 241, 459
BseLI CCNNNNNNNGG 1 cut(s) 403
BseNI ACTGG 1 cut(s) 374
BseXI GCAGC 2 cut(s) 105, 132
BshFI GGCC 1 cut(s) 553
BsiSI CCGG 1 cut(s) 205
BslI CCNNNNNNNGG 1 cut(s) 403
BsmI GAATGC 1 cut(s) 475
BsnI GGCC 1 cut(s) 553
Bsp143I GATC 1 cut(s) 175
BspACI CCGC 1 cut(s) 399
BspANI GGCC 1 cut(s) 553
BspHI TCATGA 1 cut(s) 560
BspPI GGATC 1 cut(s) 170
BsrI ACTGG 1 cut(s) 374
BssECI CCNNGG 1 cut(s) 205
BssMI GATC 1 cut(s) 175
BssSI CACGAG 1 cut(s) 314
Bst2BI CACGAG 1 cut(s) 314
Bst4CI ACNGT 2 cut(s) 261, 324
Bst6I CTCTTC 1 cut(s) 132
BstC8I GCNNGC 1 cut(s) 375
BstDEI CTNAG 1 cut(s) 351
BstF5I GGATG 4 cut(s) 137, 201, 241, 459
BstKTI GATC 1 cut(s) 178
BstMBI GATC 1 cut(s) 175
BstMWI GCNNNNNNNGC 1 cut(s) 93
BstNSI RCATGY 1 cut(s) 377
BstSCI CCNGG 1 cut(s) 204
BstSFI CTRYAG 1 cut(s) 504
BstV1I GCAGC 2 cut(s) 105, 132
BstXI CCANNNNNNTGG 1 cut(s) 132
BsuRI GGCC 1 cut(s) 553
BtsCI GGATG 4 cut(s) 137, 201, 241, 459
Cac8I GCNNGC 1 cut(s) 375
CciI TCATGA 1 cut(s) 560
CviAII CATG 4 cut(s) 90, 374, 527, 561
CviJI RGCY 7 cut(s) 82, 96, 173, 223, 313, 513, 553
CviKI_1 RGCY 7 cut(s) 82, 96, 173, 223, 313, 513, 553
DdeI CTNAG 1 cut(s) 351
DpnI GATC 1 cut(s) 177
DpnII GATC 1 cut(s) 175
EaeI YGGCCR 1 cut(s) 551
Eam1104I CTCTTC 1 cut(s) 132
EarI CTCTTC 1 cut(s) 132
Eco57I CTGAAG 1 cut(s) 144
FaeI CATG 4 cut(s) 93, 377, 530, 564
FatI CATG 4 cut(s) 89, 373, 526, 560
FauI CCCGC 1 cut(s) 406
FauNDI CATATG 2 cut(s) 25, 437
Fnu4HI GCNGC 2 cut(s) 94, 146
FokI GGATG 4 cut(s) 144, 188, 228, 446
Fsp4HI GCNGC 2 cut(s) 94, 146
FspBI CTAG 3 cut(s) 170, 345, 425
GluI GCNGC 2 cut(s) 94, 146
HaeIII GGCC 1 cut(s) 553
HapII CCGG 1 cut(s) 205
Hin1II CATG 4 cut(s) 93, 377, 530, 564
HincII GTYRAC 1 cut(s) 69
HindII GTYRAC 1 cut(s) 69
HindIII AAGCTT 2 cut(s) 221, 511
HinfI GANTC 2 cut(s) 253, 393
HpaII CCGG 1 cut(s) 205
HphI GGTGA 2 cut(s) 174, 255
Hpy166II GTNNAC 1 cut(s) 69
Hpy188I TCNGA 3 cut(s) 163, 246, 301
Hpy188III TCNNGA 1 cut(s) 561
Hpy8I GTNNAC 1 cut(s) 69
HpyAV CCTTC 1 cut(s) 422
HpyCH4III ACNGT 2 cut(s) 261, 324
HpyCH4V TGCA 8 cut(s) 4, 93, 307, 413, 441, 459, 530, 541
HpyF10VI GCNNNNNNNGC 1 cut(s) 93
HpyF3I CTNAG 1 cut(s) 351
Hsp92II CATG 4 cut(s) 93, 377, 530, 564
Kzo9I GATC 1 cut(s) 175
LpnPI CCDG 4 cut(s) 218, 355, 387, 503
Lsp1109I GCAGC 2 cut(s) 105, 132
LweI GCATC 2 cut(s) 350, 468
MaeI CTAG 3 cut(s) 170, 345, 425
MaeIII GTNAC 3 cut(s) 180, 261, 431
MalI GATC 1 cut(s) 177
MboI GATC 1 cut(s) 175
MboII GAAGA 2 cut(s) 119, 149
MfeI CAATTG 1 cut(s) 36
MlsI TGGCCA 1 cut(s) 553
MluCI AATT 8 cut(s) 36, 46, 120, 274, 452, 464, 483, 544
MluNI TGGCCA 1 cut(s) 553
MlyI GAGTC 1 cut(s) 387
MnlI CCTC 2 cut(s) 133, 348
Mox20I TGGCCA 1 cut(s) 553
MscI TGGCCA 1 cut(s) 553
MseI TTAA 1 cut(s) 522
MslI CAYNNNNRTG 2 cut(s) 14, 130
Msp20I TGGCCA 1 cut(s) 553
MspI CCGG 1 cut(s) 205
MspR9I CCNGG 1 cut(s) 206
MunI CAATTG 1 cut(s) 36
Mva1269I GAATGC 1 cut(s) 475
MwoI GCNNNNNNNGC 1 cut(s) 93
NciI CCSGG 1 cut(s) 206
NdeI CATATG 2 cut(s) 25, 437
NdeII GATC 1 cut(s) 175
NlaIII CATG 4 cut(s) 93, 377, 530, 564
NmuCI GTSAC 2 cut(s) 180, 261
NspI RCATGY 1 cut(s) 377
OliI CACNNNNGTG 1 cut(s) 14
PaeI GCATGC 1 cut(s) 377
PagI TCATGA 1 cut(s) 560
PcsI WCGNNNNNNNCGW 1 cut(s) 297
PctI GAATGC 1 cut(s) 475
PfeI GAWTC 1 cut(s) 253
PkrI GCNGC 2 cut(s) 95, 147
PleI GAGTC 1 cut(s) 387
PpsI GAGTC 1 cut(s) 387
RseI CAYNNNNRTG 2 cut(s) 14, 130
SaqAI TTAA 1 cut(s) 522
SatI GCNGC 2 cut(s) 94, 146
Sau3AI GATC 1 cut(s) 175
SchI GAGTC 1 cut(s) 387
ScrFI CCNGG 1 cut(s) 206
SetI ASST 8 cut(s) 84, 98, 144, 225, 315, 433, 515, 522
SfaNI GCATC 2 cut(s) 350, 468
SfcI CTRYAG 1 cut(s) 504
SmiMI CAYNNNNRTG 2 cut(s) 14, 130
SphI GCATGC 1 cut(s) 377
Sse9I AATT 8 cut(s) 36, 46, 120, 274, 452, 464, 483, 544
SsiI CCGC 1 cut(s) 399
SspMI CTAG 3 cut(s) 170, 345, 425
StyD4I CCNGG 1 cut(s) 204
TaaI ACNGT 2 cut(s) 261, 324
TasI AATT 8 cut(s) 36, 46, 120, 274, 452, 464, 483, 544
TfiI GAWTC 1 cut(s) 253
Tru1I TTAA 1 cut(s) 522
Tru9I TTAA 1 cut(s) 522
TseFI GTSAC 2 cut(s) 180, 261
TseI GCWGC 2 cut(s) 93, 145
Tsp45I GTSAC 2 cut(s) 180, 261
TspDTI ATGAA 3 cut(s) 126, 190, 549
TspGWI ACGGA 1 cut(s) 168
XapI RAATTY 3 cut(s) 46, 452, 544
XceI RCATGY 1 cut(s) 377
XspI CTAG 3 cut(s) 170, 345, 425
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.