pycom15g24440

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
N/A
Physical Location & Seq
Forward (+)
18611018 .. 18611437
420 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 420 bp
ATGGCAAGACTTTCACGCCACCTGGGTTCGTTGCCTCTTCTGGCAAAGCACAGGATAAATTGTATCCGAACTGCGATTAAGCGGAACATGGAGGTGCAGAATTATGCTTATTCAAAGCAGATGCTTGAACTCCTGCTATCCAAAGCACCTCCAAGTAAGCAGGAAGAGTTGAGGAGCTTAGTTGATATGTGTGTGCAAAGGGGTCTGACCAACAAGTCCATTGATCCACTAGAGGATCCTTCCCAGTTCTGTGCCGCCACACTTAGTCGTCTGTCAACTATTGGATATGACGTTTGTGATCTTTGTGGGGCCAAATTTTCAGCTCTCTCAGCACCCGGTTGCATTATTTGCGGTATGGGAAGCATTAAAAGATCAGATGCTCTCACAGGGCCAGGGCCTGTTCCTTCACCGTTTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
COPI_C

Protein Analysis

140

Amino Acids

15.04

Weight (kDa)

9.08

Isoelectric Point (pI)

54.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 214
AciI CCGC 3 cut(s) 82, 255, 351
AclWI GGATC 3 cut(s) 218, 230, 243
AcsI RAATTY 1 cut(s) 314
AgsI TTSAA 2 cut(s) 114, 128
AjnI CCWGG 2 cut(s) 21, 391
AluBI AGCT 2 cut(s) 177, 323
AluI AGCT 2 cut(s) 177, 323
AlwI GGATC 3 cut(s) 218, 230, 243
AlwNI CAGNNNCTG 1 cut(s) 398
AoxI GGCC 3 cut(s) 309, 389, 395
ApoI RAATTY 1 cut(s) 314
AspS9I GGNCC 3 cut(s) 309, 389, 395
AsuC2I CCSGG 1 cut(s) 336
AsuHPI GGTGA 1 cut(s) 399
BamHI GGATCC 1 cut(s) 235
BciT130I CCWGG 2 cut(s) 23, 393
BciVI GTATCC 1 cut(s) 74
BcnI CCSGG 1 cut(s) 336
BfaI CTAG 1 cut(s) 230
BfuI GTATCC 1 cut(s) 74
BisI GCNGC 1 cut(s) 255
BlsI GCNGC 1 cut(s) 256
Bme1390I CCNGG 3 cut(s) 23, 336, 393
BmgT120I GGNCC 3 cut(s) 309, 389, 395
BmiI GGNNCC 2 cut(s) 237, 310
BmrFI CCNGG 3 cut(s) 23, 336, 393
BmrI ACTGGG 1 cut(s) 238
BmsI GCATC 2 cut(s) 111, 367
BmuI ACTGGG 1 cut(s) 238
BpuMI CCSGG 1 cut(s) 336
BsaJI CCNNGG 2 cut(s) 22, 392
Bse1I ACTGG 1 cut(s) 244
BseBI CCWGG 2 cut(s) 23, 393
BseDI CCNNGG 2 cut(s) 22, 392
BseMII CTCAG 1 cut(s) 342
BseNI ACTGG 1 cut(s) 244
BseRI GAGGAG 1 cut(s) 187
BsgI GTGCAG 1 cut(s) 116
BshFI GGCC 3 cut(s) 311, 391, 397
BsiSI CCGG 1 cut(s) 336
BsnI GGCC 3 cut(s) 311, 391, 397
Bsp143I GATC 4 cut(s) 223, 235, 298, 371
BspACI CCGC 3 cut(s) 82, 255, 351
BspANI GGCC 3 cut(s) 311, 391, 397
BspCNI CTCAG 1 cut(s) 341
BspLI GGNNCC 2 cut(s) 237, 310
BspPI GGATC 3 cut(s) 218, 230, 243
BsrI ACTGG 1 cut(s) 244
BssECI CCNNGG 2 cut(s) 22, 392
BssMI GATC 4 cut(s) 223, 235, 298, 371
Bst2UI CCWGG 2 cut(s) 23, 393
Bst4CI ACNGT 1 cut(s) 411
Bst6I CTCTTC 2 cut(s) 42, 159
BstAPI GCANNNNNTGC 1 cut(s) 348
BstDEI CTNAG 3 cut(s) 178, 263, 328
BstKTI GATC 4 cut(s) 226, 238, 301, 374
BstMBI GATC 4 cut(s) 223, 235, 298, 371
BstMWI GCNNNNNNNGC 2 cut(s) 329, 348
BstNI CCWGG 2 cut(s) 23, 393
BstSCI CCNGG 3 cut(s) 21, 334, 391
BstX2I RGATCY 1 cut(s) 235
BstYI RGATCY 1 cut(s) 235
BsuI GTATCC 1 cut(s) 74
BsuRI GGCC 3 cut(s) 311, 391, 397
CaiI CAGNNNCTG 1 cut(s) 398
Cfr13I GGNCC 3 cut(s) 309, 389, 395
CviAII CATG 1 cut(s) 88
CviJI RGCY 5 cut(s) 177, 311, 323, 391, 397
CviKI_1 RGCY 5 cut(s) 177, 311, 323, 391, 397
DdeI CTNAG 3 cut(s) 178, 263, 328
DpnI GATC 4 cut(s) 225, 237, 300, 373
DpnII GATC 4 cut(s) 223, 235, 298, 371
DrdI GACNNNNNNGTC 1 cut(s) 214
DseDI GACNNNNNNGTC 1 cut(s) 214
Eam1104I CTCTTC 2 cut(s) 42, 159
EarI CTCTTC 2 cut(s) 42, 159
EcoO109I RGGNCCY 1 cut(s) 395
EcoRII CCWGG 2 cut(s) 21, 391
FaeI CATG 1 cut(s) 91
FaiI YATR 5 cut(s) 89, 105, 188, 288, 356
FatI CATG 1 cut(s) 87
Fnu4HI GCNGC 1 cut(s) 255
Fsp4HI GCNGC 1 cut(s) 255
FspBI CTAG 1 cut(s) 230
GluI GCNGC 1 cut(s) 255
HaeIII GGCC 3 cut(s) 311, 391, 397
HapII CCGG 1 cut(s) 336
Hin1II CATG 1 cut(s) 91
HincII GTYRAC 1 cut(s) 276
HindII GTYRAC 1 cut(s) 276
HpaII CCGG 1 cut(s) 336
HphI GGTGA 1 cut(s) 399
Hpy166II GTNNAC 1 cut(s) 276
Hpy188I TCNGA 3 cut(s) 68, 207, 376
Hpy8I GTNNAC 1 cut(s) 276
HpyAV CCTTC 2 cut(s) 249, 414
HpyCH4III ACNGT 1 cut(s) 411
HpyCH4IV ACGT 1 cut(s) 291
HpyCH4V TGCA 3 cut(s) 97, 196, 342
HpyF10VI GCNNNNNNNGC 2 cut(s) 329, 348
HpyF3I CTNAG 3 cut(s) 178, 263, 328
HpySE526I ACGT 1 cut(s) 291
Hsp92II CATG 1 cut(s) 91
Kzo9I GATC 4 cut(s) 223, 235, 298, 371
LmnI GCTCC 1 cut(s) 174
LweI GCATC 2 cut(s) 111, 367
MaeI CTAG 1 cut(s) 230
MaeII ACGT 1 cut(s) 291
MalI GATC 4 cut(s) 225, 237, 300, 373
MboI GATC 4 cut(s) 223, 235, 298, 371
MboII GAAGA 2 cut(s) 29, 176
MflI RGATCY 1 cut(s) 235
MluCI AATT 3 cut(s) 58, 100, 314
MnlI CCTC 5 cut(s) 45, 85, 159, 165, 226
MseI TTAA 2 cut(s) 78, 366
MslI CAYNNNNRTG 1 cut(s) 92
MspI CCGG 1 cut(s) 336
MspR9I CCNGG 3 cut(s) 23, 336, 393
MvaI CCWGG 2 cut(s) 23, 393
MwoI GCNNNNNNNGC 2 cut(s) 329, 348
NciI CCSGG 1 cut(s) 336
NdeII GATC 4 cut(s) 223, 235, 298, 371
NlaIII CATG 1 cut(s) 91
NlaIV GGNNCC 2 cut(s) 237, 310
PkrI GCNGC 1 cut(s) 256
Psp6I CCWGG 2 cut(s) 21, 391
PspGI CCWGG 2 cut(s) 21, 391
PspN4I GGNNCC 2 cut(s) 237, 310
PspPI GGNCC 3 cut(s) 309, 389, 395
PstNI CAGNNNCTG 1 cut(s) 398
PsuI RGATCY 1 cut(s) 235
RseI CAYNNNNRTG 1 cut(s) 92
SaqAI TTAA 2 cut(s) 78, 366
SatI GCNGC 1 cut(s) 255
Sau3AI GATC 4 cut(s) 223, 235, 298, 371
Sau96I GGNCC 3 cut(s) 309, 389, 395
ScrFI CCNGG 3 cut(s) 23, 336, 393
SetI ASST 6 cut(s) 24, 96, 151, 179, 294, 325
SfaNI GCATC 2 cut(s) 111, 367
SmiMI CAYNNNNRTG 1 cut(s) 92
Sse9I AATT 3 cut(s) 58, 100, 314
SsiI CCGC 3 cut(s) 82, 255, 351
SspMI CTAG 1 cut(s) 230
StyD4I CCNGG 3 cut(s) 21, 334, 391
TaaI ACNGT 1 cut(s) 411
TaiI ACGT 1 cut(s) 294
TasI AATT 3 cut(s) 58, 100, 314
TauI GCSGC 1 cut(s) 257
Tru1I TTAA 2 cut(s) 78, 366
Tru9I TTAA 2 cut(s) 78, 366
XapI RAATTY 1 cut(s) 314
XspI CTAG 1 cut(s) 230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.