pycom15g24880

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
N/A
Physical Location & Seq
Forward (+)
19193919 .. 19195298
1380 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 690 bp
ATGGAGGGAGAGGCAGAGATTGATCGGTTGCCAATGGACCTCTTGGCGCATATATTTGTTCTCATAACTTCCTTCACTGATTTGGCCCATGTCATTTATCACGATTACTCAAATAACTCACCTCCAAGTTTTTGTGTTGGCTGTTATGTGTTAGAGATTCTCCGGGTTAGAAGTGCTTTTTATTTTGCCTTTTCATCAGATTTGCATCTGATTACTCTCATTTCAAGATTTTGTTCTTCCGAGATTTCAAGGAGTCGTTGGAGTTGCCAGATAACCGACACTGGATTGTACAGAATATCTATGGCAAAGTGTATTGGCAACTTAACATCAATATCACTATGGGGCATCACAGGGATAACGGATAAAGGTGTTGTTCAACTGATATCAAGAGCTAATTCCTTACACCACCTGAATATCGGTGGCACATTTATCACAGATGACTCGTTATATGCCGTTGCTCATAGCTGTCGGCATTTGAAGACTGTCATCCTGTGGAGCTGCCGTCATGTGACCGAGACCGGCCTCTTTGTTCTTGTAAAATATTGCCGCAAGCTTGAATCCATCAATTTGTGGGGGACGAGAGTTCCTGTAGACTGCTTCCTTGCTTTGATCACTATCAGTCCAGCTCTACAGATAAAACCGCGAGGACTGTCGTTAAATGTGGACGCTTCTATGTTGCCCATTGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box-like LRR_6

Protein Analysis

230

Amino Acids

25.6

Weight (kDa)

7.61

Isoelectric Point (pI)

44.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7885 PF25372 65 - 180 3.4e-08 Leucine Rich Repeat Domain of unknown function (DUF7885)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 591
AccII CGCG 1 cut(s) 643
AciI CCGC 2 cut(s) 547, 641
AfaI GTAC 1 cut(s) 290
AgsI TTSAA 5 cut(s) 225, 249, 377, 478, 557
AluBI AGCT 5 cut(s) 392, 465, 498, 553, 626
AluI AGCT 5 cut(s) 392, 465, 498, 553, 626
Alw26I GTCTC 1 cut(s) 509
AoxI GGCC 2 cut(s) 84, 520
ApeKI GCWGC 1 cut(s) 498
AspLEI GCGC 1 cut(s) 49
AspS9I GGNCC 2 cut(s) 37, 85
AsuC2I CCSGG 1 cut(s) 164
AsuHPI GGTGA 1 cut(s) 111
AvaII GGWCC 1 cut(s) 37
BbsI GAAGAC 1 cut(s) 485
BbvI GCAGC 1 cut(s) 485
BccI CCATC 1 cut(s) 569
BceAI ACGGC 2 cut(s) 437, 486
BclI TGATCA 1 cut(s) 609
BcnI CCSGG 1 cut(s) 164
BcoDI GTCTC 1 cut(s) 509
BfmI CTRYAG 2 cut(s) 588, 629
BisI GCNGC 2 cut(s) 499, 547
BlsI GCNGC 2 cut(s) 500, 548
Bme1390I CCNGG 1 cut(s) 164
Bme18I GGWCC 1 cut(s) 37
BmgT120I GGNCC 2 cut(s) 37, 85
BmrFI CCNGG 1 cut(s) 164
BmsI GCATC 2 cut(s) 214, 354
BpiI GAAGAC 1 cut(s) 485
BpuMI CCSGG 1 cut(s) 164
BsaBI GATNNNNATC 2 cut(s) 204, 614
BsaI GGTCTC 1 cut(s) 509
BsaXI ACNNNNNCTCC 2 cut(s) 487, 517
Bse118I RCCGGY 1 cut(s) 518
Bse1I ACTGG 1 cut(s) 286
Bse8I GATNNNNATC 2 cut(s) 204, 614
BseGI GGATG 1 cut(s) 486
BseJI GATNNNNATC 2 cut(s) 204, 614
BseNI ACTGG 1 cut(s) 286
BseXI GCAGC 1 cut(s) 485
Bsh1236I CGCG 1 cut(s) 643
BshFI GGCC 2 cut(s) 86, 522
BsiSI CCGG 2 cut(s) 163, 519
BslFI GGGAC 1 cut(s) 589
BsmAI GTCTC 1 cut(s) 509
BsmFI GGGAC 1 cut(s) 589
BsnI GGCC 2 cut(s) 86, 522
Bso31I GGTCTC 1 cut(s) 509
Bsp1407I TGTACA 1 cut(s) 288
Bsp143I GATC 2 cut(s) 22, 609
BspACI CCGC 2 cut(s) 547, 641
BspANI GGCC 2 cut(s) 86, 522
BspFNI CGCG 1 cut(s) 643
BspTNI GGTCTC 1 cut(s) 509
BsrFI RCCGGY 1 cut(s) 518
BsrGI TGTACA 1 cut(s) 288
BsrI ACTGG 1 cut(s) 286
BssAI RCCGGY 1 cut(s) 518
BssMI GATC 2 cut(s) 22, 609
Bst4CI ACNGT 2 cut(s) 484, 651
BstAUI TGTACA 1 cut(s) 288
BstC8I GCNNGC 1 cut(s) 551
BstF5I GGATG 1 cut(s) 486
BstFNI CGCG 1 cut(s) 643
BstHHI GCGC 1 cut(s) 49
BstKTI GATC 2 cut(s) 25, 612
BstMAI GTCTC 1 cut(s) 509
BstMBI GATC 2 cut(s) 22, 609
BstSCI CCNGG 1 cut(s) 162
BstSFI CTRYAG 2 cut(s) 588, 629
BstUI CGCG 1 cut(s) 643
BstV1I GCAGC 1 cut(s) 485
BstV2I GAAGAC 1 cut(s) 485
BsuRI GGCC 2 cut(s) 86, 522
BtsCI GGATG 1 cut(s) 486
BtsIMutI CAGTG 2 cut(s) 75, 279
Cac8I GCNNGC 1 cut(s) 551
CfoI GCGC 1 cut(s) 49
Cfr10I RCCGGY 1 cut(s) 518
Cfr13I GGNCC 2 cut(s) 37, 85
CseI GACGC 1 cut(s) 674
Csp6I GTAC 1 cut(s) 289
CviAII CATG 2 cut(s) 89, 506
CviJI RGCY 8 cut(s) 86, 141, 392, 465, 498, 522, 553, 626
CviKI_1 RGCY 8 cut(s) 86, 141, 392, 465, 498, 522, 553, 626
CviQI GTAC 1 cut(s) 289
DpnI GATC 2 cut(s) 24, 611
DpnII GATC 2 cut(s) 22, 609
Eco31I GGTCTC 1 cut(s) 509
Eco32I GATATC 1 cut(s) 384
Eco47I GGWCC 1 cut(s) 37
EcoRV GATATC 1 cut(s) 384
FaeI CATG 2 cut(s) 92, 509
FaqI GGGAC 1 cut(s) 589
FatI CATG 2 cut(s) 88, 505
FbaI TGATCA 1 cut(s) 609
FblI GTMKAC 1 cut(s) 591
Fnu4HI GCNGC 2 cut(s) 499, 547
FokI GGATG 1 cut(s) 473
Fsp4HI GCNGC 2 cut(s) 499, 547
GlaI GCGC 1 cut(s) 48
GluI GCNGC 2 cut(s) 499, 547
HaeIII GGCC 2 cut(s) 86, 522
HapII CCGG 2 cut(s) 163, 519
HgaI GACGC 1 cut(s) 674
HhaI GCGC 1 cut(s) 49
Hin1II CATG 2 cut(s) 92, 509
Hin6I GCGC 1 cut(s) 47
HinP1I GCGC 1 cut(s) 47
HindIII AAGCTT 1 cut(s) 551
HinfI GANTC 4 cut(s) 157, 253, 440, 557
HpaII CCGG 2 cut(s) 163, 519
HphI GGTGA 1 cut(s) 111
Hpy166II GTNNAC 2 cut(s) 592, 664
Hpy188I TCNGA 3 cut(s) 199, 210, 241
Hpy188III TCNNGA 3 cut(s) 101, 225, 387
Hpy8I GTNNAC 2 cut(s) 592, 664
HpyAV CCTTC 1 cut(s) 82
HpyCH4III ACNGT 2 cut(s) 484, 651
HpyCH4V TGCA 1 cut(s) 205
Hsp92II CATG 2 cut(s) 92, 509
HspAI GCGC 1 cut(s) 47
Ksp22I TGATCA 1 cut(s) 609
Kzo9I GATC 2 cut(s) 22, 609
LmnI GCTCC 1 cut(s) 495
LpnPI CCDG 9 cut(s) 176, 267, 281, 336, 422, 503, 532, 600, 636
Lsp1109I GCAGC 1 cut(s) 485
LweI GCATC 2 cut(s) 214, 354
MaeIII GTNAC 1 cut(s) 508
MalI GATC 2 cut(s) 24, 611
MboI GATC 2 cut(s) 22, 609
MboII GAAGA 2 cut(s) 228, 490
MluCI AATT 2 cut(s) 394, 565
MlyI GAGTC 2 cut(s) 262, 434
MmeI TCCRAC 1 cut(s) 239
MnlI CCTC 5 cut(s) 4, 50, 132, 533, 638
MseI TTAA 2 cut(s) 323, 656
MspI CCGG 2 cut(s) 163, 519
MspR9I CCNGG 1 cut(s) 164
MvnI CGCG 1 cut(s) 643
NciI CCSGG 1 cut(s) 164
NdeII GATC 2 cut(s) 22, 609
NlaIII CATG 2 cut(s) 92, 509
NmuCI GTSAC 1 cut(s) 508
PfeI GAWTC 2 cut(s) 157, 557
PkrI GCNGC 2 cut(s) 500, 548
PleI GAGTC 2 cut(s) 261, 434
PpsI GAGTC 2 cut(s) 261, 434
PspPI GGNCC 2 cut(s) 37, 85
RsaI GTAC 1 cut(s) 290
RsaNI GTAC 1 cut(s) 289
SaqAI TTAA 2 cut(s) 323, 656
SatI GCNGC 2 cut(s) 499, 547
Sau3AI GATC 2 cut(s) 22, 609
Sau96I GGNCC 2 cut(s) 37, 85
SchI GAGTC 2 cut(s) 262, 434
ScrFI CCNGG 1 cut(s) 164
SetI ASST 9 cut(s) 42, 124, 370, 394, 411, 467, 500, 555, 628
SfaNI GCATC 2 cut(s) 214, 354
SfcI CTRYAG 2 cut(s) 588, 629
SinI GGWCC 1 cut(s) 37
Sse9I AATT 2 cut(s) 394, 565
SsiI CCGC 2 cut(s) 547, 641
SspI AATATT 1 cut(s) 542
StyD4I CCNGG 1 cut(s) 162
TaaI ACNGT 2 cut(s) 484, 651
TaqII GACCGA 1 cut(s) 527
TasI AATT 2 cut(s) 394, 565
TatI WGTACW 1 cut(s) 288
TauI GCSGC 1 cut(s) 549
TfiI GAWTC 2 cut(s) 157, 557
Tru1I TTAA 2 cut(s) 323, 656
Tru9I TTAA 2 cut(s) 323, 656
TscAI CASTG 2 cut(s) 82, 286
TseFI GTSAC 1 cut(s) 508
TseI GCWGC 1 cut(s) 498
Tsp45I GTSAC 1 cut(s) 508
TspDTI ATGAA 1 cut(s) 183
TspGWI ACGGA 1 cut(s) 374
TspRI CASTG 2 cut(s) 82, 286
VpaK11BI GGWCC 1 cut(s) 37
XmiI GTMKAC 1 cut(s) 591
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.