Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr15

Orthogroup ID

N/A

Physical Location & Seq

Genomic Coordinates Forward (+)

24670618 .. 24672560

Gene Structure 1943 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 654 bp

ATGTTTCTTATGTCTCGAAAAATTAAATCTTTGGGAGTAAAGATGGTTATTTCTGGGGAAGGTTCAGATGAAATTTTTGGAGGTTACTTGTATTTCCATAAGGCACCAAACAAGGAGGAGTTTCACCAAGAAACATGTCAGAAGATTAAAGCTCTTCATCTGTATGACTGTCTGAGGGCCAATAAATCAACTTCAGCTTGGGGTGTTGAGGCTTGTGTGCCCTTTTTGGATAAAGAATTCATCAATATTGCCATGAACATTGATCCAGAATGGAAGATGATCAGGCTTGGGAGAATTGAGAAGTGGGTCTTACGCAATGCATTTGATGATGATCAGAAGCCATATTTGCAGAAGCACATATTGTACAGGCAGAAGGAACAGTTCACTGATGGCGTAGGCTACAGTTGGATTGATGGCTTGAAAGATCATGCAAACAGTCAAATTGCTGCCAGATCAACAGTTCCCGGTGGTCCAAGTGTGGCATGCAGTACAGCAAAAGCTGTGGAATGGGATGCAGAATGGTCAAAAAGGATCCTGATCCATCTGGTCCGGCTGCACTTGGTATTCATGCAGCTGCGTACGAGGAGGCAGCAGGGAATGCAAGTAATGGAAATGTTCTCAGTGGCTCTCAAAAAGTACAAGAAGGGATTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004066 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005911 GO:0006082 GO:0006520 GO:0006528 GO:0006529 GO:0006807 GO:0006950 GO:0006970 GO:0006972 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009066 GO:0009067 GO:0009314 GO:0009416 GO:0009506 GO:0009628 GO:0009642 GO:0009646 GO:0009651 GO:0009987 GO:0016053 GO:0016874 GO:0016879 GO:0016884 GO:0019752 GO:0030054 GO:0032787 GO:0034641 GO:0042538 GO:0042802 GO:0042803 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0046983 GO:0050896 GO:0055044 GO:0071704 GO:0072330 GO:0097164 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00250 ko01100 ko01110 map00250 map01100 map01110

Pfam Domains

Protein Families

Asn_synthase GATase_7

Protein Analysis

218

Amino Acids

25.12

Weight (kDa)

9.36

Isoelectric Point (pI)

50.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Asn_synthase	PF00733	1 - 38	2.2e-10	Asparagine synthase
Asn_synthase	PF00733	36 - 152	1.9e-26	Asparagine synthase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	103
AclWI	GGATC	4 cut(s)	257, 526, 532, 539
AcsI	RAATTY	2 cut(s)	72, 236
AcuI	CTGAAG	1 cut(s)	177
AfaI	GTAC	4 cut(s)	365, 490, 580, 638
AflIII	ACRYGT	1 cut(s)	134
AgsI	TTSAA	1 cut(s)	421
AluBI	AGCT	4 cut(s)	152, 197, 500, 574
AluI	AGCT	4 cut(s)	152, 197, 500, 574
Alw26I	GTCTC	1 cut(s)	18
AlwI	GGATC	4 cut(s)	257, 526, 532, 539
AoxI	GGCC	1 cut(s)	177
ApeKI	GCWGC	5 cut(s)	446, 553, 571, 574, 589
ApoI	RAATTY	2 cut(s)	72, 236
AspS9I	GGNCC	3 cut(s)	177, 470, 547
AsuC2I	CCSGG	1 cut(s)	465
AsuHPI	GGTGA	1 cut(s)	116
AvaII	GGWCC	2 cut(s)	470, 547
BaeGI	GKGCMC	1 cut(s)	222
BamHI	GGATCC	1 cut(s)	531
BanI	GGYRCC	1 cut(s)	103
BbvI	GCAGC	5 cut(s)	433, 540, 561, 583, 601
BccI	CCATC	4 cut(s)	37, 383, 407, 549
BclI	TGATCA	2 cut(s)	279, 331
BcnI	CCSGG	1 cut(s)	465
BcoDI	GTCTC	1 cut(s)	18
BfmI	CTRYAG	1 cut(s)	400
BisI	GCNGC	5 cut(s)	447, 554, 572, 575, 590
BlsI	GCNGC	5 cut(s)	448, 555, 573, 576, 591
Bme1390I	CCNGG	1 cut(s)	465
Bme18I	GGWCC	2 cut(s)	470, 547
BmgT120I	GGNCC	3 cut(s)	177, 470, 547
BmiI	GGNNCC	2 cut(s)	105, 533
BmrFI	CCNGG	1 cut(s)	465
BmsI	GCATC	1 cut(s)	502
BpuMI	CCSGG	1 cut(s)	465
BsaBI	GATNNNNATC	2 cut(s)	330, 536
Bse3DI	GCAATG	1 cut(s)	322
Bse8I	GATNNNNATC	2 cut(s)	330, 536
BseGI	GGATG	1 cut(s)	517
BseJI	GATNNNNATC	2 cut(s)	330, 536
BseMI	GCAATG	1 cut(s)	322
BseMII	CTCAG	2 cut(s)	164, 633
BseRI	GAGGAG	2 cut(s)	131, 598
BseSI	GKGCMC	1 cut(s)	222
BseXI	GCAGC	5 cut(s)	433, 540, 561, 583, 601
BsgI	GTGCAG	1 cut(s)	539
BshFI	GGCC	1 cut(s)	179
BshNI	GGYRCC	1 cut(s)	103
BsiSI	CCGG	2 cut(s)	465, 550
BsiWI	CGTACG	1 cut(s)	578
BsmAI	GTCTC	1 cut(s)	18
BsmI	GAATGC	1 cut(s)	603
BsnI	GGCC	1 cut(s)	179
Bsp1286I	GDGCHC	1 cut(s)	222
Bsp1407I	TGTACA	1 cut(s)	363
Bsp143I	GATC	7 cut(s)	262, 279, 331, 424, 452, 531, 537
BspANI	GGCC	1 cut(s)	179
BspCNI	CTCAG	2 cut(s)	165, 632
BspLI	GGNNCC	2 cut(s)	105, 533
BspPI	GGATC	4 cut(s)	257, 526, 532, 539
BspQI	GCTCTTC	1 cut(s)	159
BspT107I	GGYRCC	1 cut(s)	103
BsrDI	GCAATG	1 cut(s)	322
BsrGI	TGTACA	1 cut(s)	363
BssMI	GATC	7 cut(s)	262, 279, 331, 424, 452, 531, 537
Bst4CI	ACNGT	5 cut(s)	170, 381, 404, 437, 460
Bst6I	CTCTTC	1 cut(s)	159
BstAPI	GCANNNNNTGC	1 cut(s)	598
BstAUI	TGTACA	1 cut(s)	363
BstC8I	GCNNGC	1 cut(s)	484
BstDEI	CTNAG	2 cut(s)	173, 619
BstF5I	GGATG	1 cut(s)	517
BstKTI	GATC	7 cut(s)	265, 282, 334, 427, 455, 534, 540
BstMAI	GTCTC	1 cut(s)	18
BstMBI	GATC	7 cut(s)	262, 279, 331, 424, 452, 531, 537
BstMWI	GCNNNNNNNGC	2 cut(s)	346, 598
BstNSI	RCATGY	2 cut(s)	138, 486
BstSCI	CCNGG	1 cut(s)	463
BstSFI	CTRYAG	1 cut(s)	400
BstSLI	GKGCMC	1 cut(s)	222
BstV1I	GCAGC	5 cut(s)	433, 540, 561, 583, 601
BstX2I	RGATCY	1 cut(s)	531
BstYI	RGATCY	1 cut(s)	531
BsuRI	GGCC	1 cut(s)	179
BtsCI	GGATG	1 cut(s)	517
BtsIMutI	CAGTG	2 cut(s)	384, 627
Cac8I	GCNNGC	1 cut(s)	484
Cfr13I	GGNCC	3 cut(s)	177, 470, 547
Csp6I	GTAC	4 cut(s)	364, 489, 579, 637
CspCI	CAANNNNNGTGG	2 cut(s)	483, 518
CviAII	CATG	5 cut(s)	135, 253, 428, 483, 568
CviQI	GTAC	4 cut(s)	364, 489, 579, 637
DdeI	CTNAG	2 cut(s)	173, 619
DpnI	GATC	7 cut(s)	264, 281, 333, 426, 454, 533, 539
DpnII	GATC	7 cut(s)	262, 279, 331, 424, 452, 531, 537
Eam1104I	CTCTTC	1 cut(s)	159
EarI	CTCTTC	1 cut(s)	159
Eco47I	GGWCC	2 cut(s)	470, 547
Eco57I	CTGAAG	1 cut(s)	177
EcoRI	GAATTC	1 cut(s)	236
EcoT22I	ATGCAT	1 cut(s)	322
FaeI	CATG	5 cut(s)	138, 256, 431, 486, 571
FalI	AAGNNNNNCTT	2 cut(s)	293, 325
FatI	CATG	5 cut(s)	134, 252, 427, 482, 567
FbaI	TGATCA	2 cut(s)	279, 331
Fnu4HI	GCNGC	5 cut(s)	447, 554, 572, 575, 590
FokI	GGATG	1 cut(s)	524
Fsp4HI	GCNGC	5 cut(s)	447, 554, 572, 575, 590
GluI	GCNGC	5 cut(s)	447, 554, 572, 575, 590
HaeIII	GGCC	1 cut(s)	179
HapII	CCGG	2 cut(s)	465, 550
Hin1II	CATG	5 cut(s)	138, 256, 431, 486, 571
HpaII	CCGG	2 cut(s)	465, 550
HphI	GGTGA	1 cut(s)	116
Hpy166II	GTNNAC	1 cut(s)	384
Hpy188I	TCNGA	4 cut(s)	67, 141, 174, 336
Hpy188III	TCNNGA	3 cut(s)	15, 266, 535
Hpy8I	GTNNAC	1 cut(s)	384
HpyAV	CCTTC	3 cut(s)	53, 367, 637
HpyCH4III	ACNGT	5 cut(s)	170, 381, 404, 437, 460
HpyCH4V	TGCA	8 cut(s)	320, 349, 431, 486, 515, 556, 571, 601
HpyF10VI	GCNNNNNNNGC	2 cut(s)	346, 598
HpyF3I	CTNAG	2 cut(s)	173, 619
Hsp92II	CATG	5 cut(s)	138, 256, 431, 486, 571
Ksp22I	TGATCA	2 cut(s)	279, 331
Kzo9I	GATC	7 cut(s)	262, 279, 331, 424, 452, 531, 537
LguI	GCTCTTC	1 cut(s)	159
Lsp1109I	GCAGC	5 cut(s)	433, 540, 561, 583, 601
LweI	GCATC	1 cut(s)	502
MaeIII	GTNAC	1 cut(s)	83
MalI	GATC	7 cut(s)	264, 281, 333, 426, 454, 533, 539
MboI	GATC	7 cut(s)	262, 279, 331, 424, 452, 531, 537
MboII	GAAGA	3 cut(s)	146, 154, 286
MflI	RGATCY	1 cut(s)	531
MhlI	GDGCHC	1 cut(s)	222
MluCI	AATT	5 cut(s)	21, 72, 236, 294, 441
MmeI	TCCRAC	1 cut(s)	386
MnlI	CCTC	6 cut(s)	74, 109, 168, 202, 576, 579
Mph1103I	ATGCAT	1 cut(s)	322
MseI	TTAA	2 cut(s)	24, 147
MslI	CAYNNNNRTG	1 cut(s)	162
MspA1I	CMGCKG	1 cut(s)	574
MspI	CCGG	2 cut(s)	465, 550
MspR9I	CCNGG	1 cut(s)	465
Mva1269I	GAATGC	1 cut(s)	603
MwoI	GCNNNNNNNGC	2 cut(s)	346, 598
NciI	CCSGG	1 cut(s)	465
NdeII	GATC	7 cut(s)	262, 279, 331, 424, 452, 531, 537
NlaIII	CATG	5 cut(s)	138, 256, 431, 486, 571
NlaIV	GGNNCC	2 cut(s)	105, 533
NsiI	ATGCAT	1 cut(s)	322
NspI	RCATGY	2 cut(s)	138, 486
PaeI	GCATGC	1 cut(s)	486
PciI	ACATGT	1 cut(s)	134
PciSI	GCTCTTC	1 cut(s)	159
PctI	GAATGC	1 cut(s)	603
Pfl23II	CGTACG	1 cut(s)	578
PkrI	GCNGC	5 cut(s)	448, 555, 573, 576, 591
PscI	ACATGT	1 cut(s)	134
PspLI	CGTACG	1 cut(s)	578
PspN4I	GGNNCC	2 cut(s)	105, 533
PspPI	GGNCC	3 cut(s)	177, 470, 547
PsuI	RGATCY	1 cut(s)	531
PvuII	CAGCTG	1 cut(s)	574
RsaI	GTAC	4 cut(s)	365, 490, 580, 638
RsaNI	GTAC	4 cut(s)	364, 489, 579, 637
RseI	CAYNNNNRTG	1 cut(s)	162
SapI	GCTCTTC	1 cut(s)	159
SaqAI	TTAA	2 cut(s)	24, 147
SatI	GCNGC	5 cut(s)	447, 554, 572, 575, 590
Sau3AI	GATC	7 cut(s)	262, 279, 331, 424, 452, 531, 537
Sau96I	GGNCC	3 cut(s)	177, 470, 547
ScrFI	CCNGG	1 cut(s)	465
SduI	GDGCHC	1 cut(s)	222
SetI	ASST	6 cut(s)	64, 85, 154, 199, 502, 576
SfaNI	GCATC	1 cut(s)	502
SfcI	CTRYAG	1 cut(s)	400
SinI	GGWCC	2 cut(s)	470, 547
SmiMI	CAYNNNNRTG	1 cut(s)	162
SphI	GCATGC	1 cut(s)	486
Sse9I	AATT	5 cut(s)	21, 72, 236, 294, 441
SspI	AATATT	1 cut(s)	247
StyD4I	CCNGG	1 cut(s)	463
TaaI	ACNGT	5 cut(s)	170, 381, 404, 437, 460
TaqI	TCGA	1 cut(s)	16
TasI	AATT	5 cut(s)	21, 72, 236, 294, 441
TatI	WGTACW	3 cut(s)	363, 488, 636
Tru1I	TTAA	2 cut(s)	24, 147
Tru9I	TTAA	2 cut(s)	24, 147
TscAI	CASTG	2 cut(s)	391, 627
TseI	GCWGC	5 cut(s)	446, 553, 571, 574, 589
TspDTI	ATGAA	5 cut(s)	84, 146, 229, 269, 556
TspRI	CASTG	2 cut(s)	391, 627
VpaK11BI	GGWCC	2 cut(s)	470, 547
XapI	RAATTY	2 cut(s)	72, 236
XceI	RCATGY	2 cut(s)	138, 486
Zsp2I	ATGCAT	1 cut(s)	322

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.