pycom15g30840

Metal transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
N/A
Physical Location & Seq
Forward (+)
28857890 .. 28859574
1685 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 687 bp
ATGGGCTGGACCATATACCAACAGCAGAATGTGTTGGGACGATCAAGCAAAATCTTGTATGCCATTGCACTATTAGCCTCAGGCCAAAGCTCCACGATTACAGGCACTTACGCAGGACAATTTGTCATGCAGGGTTTCTTGAACATTAAGATGAAAAAATGGGCTAGAAACTTAATGACGAGGTGCATTGCCATTACACCAAGCCTCATTGTTTCCATTATCGACGGATCTTCAGGAGCAGGAAGGCTCATTATCATTGCATCGATGATACTATCTTTCGAGCTCCCATTTGCTTTAATTCCACTCCTCAAATTCAGCAGTAGTGCCACCAAAATGGGACCTCACAAGAATTCAATCTATATCATTGTGACATCATGGATTCTAGGAATGGGAATCATAGGCATCAACATCTACTACCTCAGCACAGGATTTGTAGGGTGGATAATCCACAGCAGTCTACCCAAAGTTGCAACTGTGTTCATTGGGATCTTGGTGTTCCCTTTCATGGCCATTTACATCCTTGCAGTCCTTTACCTAACCCTAAGAAAAGACAGTGTGGTAACTTTTGTTGAGCCCACAAAGAACGACCCGGCAGCCCAAAACCAAATGGAAAATGGGCTTCCCAACTATGGTGAGCCCCCAGTGCCTTTCAGAGAGGACTTGGCTGACATACCTTTTCCTCAATAG

Protein Analysis

229

Amino Acids

25.04

Weight (kDa)

9.46

Isoelectric Point (pI)

44.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nramp PF01566 10 - 118 2.7e-33 Natural resistance-associated macrophage protein-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 457
AclWI GGATC 2 cut(s) 235, 494
AcoI YGGCCR 1 cut(s) 507
AcsI RAATTY 2 cut(s) 311, 349
AcuI CTGAAG 1 cut(s) 216
AfiI CCNNNNNNNGG 2 cut(s) 505, 629
AgsI TTSAA 2 cut(s) 142, 354
AhdI GACNNNNNGTC 1 cut(s) 122
AluBI AGCT 2 cut(s) 90, 283
AluI AGCT 2 cut(s) 90, 283
Alw21I GWGCWC 1 cut(s) 285
AlwI GGATC 2 cut(s) 235, 494
AoxI GGCC 2 cut(s) 82, 507
ApeKI GCWGC 1 cut(s) 593
ApoI RAATTY 2 cut(s) 311, 349
AspS9I GGNCC 2 cut(s) 9, 338
AsuC2I CCSGG 1 cut(s) 590
AsuHPI GGTGA 1 cut(s) 644
AvaII GGWCC 2 cut(s) 9, 338
AxyI CCTNAGG 1 cut(s) 79
BalI TGGCCA 1 cut(s) 509
BanII GRGCYC 3 cut(s) 285, 576, 639
Bbv12I GWGCWC 1 cut(s) 285
BbvCI CCTCAGC 1 cut(s) 419
BbvI GCAGC 1 cut(s) 605
BcnI CCSGG 1 cut(s) 590
BfaI CTAG 2 cut(s) 165, 383
BisI GCNGC 1 cut(s) 594
BlsI GCNGC 1 cut(s) 595
Bme1390I CCNGG 1 cut(s) 590
Bme18I GGWCC 2 cut(s) 9, 338
BmeRI GACNNNNNGTC 1 cut(s) 122
BmgT120I GGNCC 2 cut(s) 9, 338
BmiI GGNNCC 1 cut(s) 339
BmrFI CCNGG 1 cut(s) 590
BmrI ACTGGG 1 cut(s) 635
BmsI GCATC 2 cut(s) 269, 411
BmuI ACTGGG 1 cut(s) 635
Bpu10I CCTNAGC 1 cut(s) 419
BpuMI CCSGG 1 cut(s) 590
Bsa29I ATCGAT 1 cut(s) 263
Bsc4I CCNNNNNNNGG 2 cut(s) 505, 629
Bse1I ACTGG 1 cut(s) 641
Bse21I CCTNAGG 1 cut(s) 79
Bse3DI GCAATG 3 cut(s) 63, 186, 255
BseCI ATCGAT 1 cut(s) 263
BseGI GGATG 1 cut(s) 516
BseLI CCNNNNNNNGG 2 cut(s) 505, 629
BseMI GCAATG 3 cut(s) 63, 186, 255
BseMII CTCAG 2 cut(s) 93, 433
BseNI ACTGG 1 cut(s) 641
BseRI GAGGAG 1 cut(s) 296
BseXI GCAGC 1 cut(s) 605
BshFI GGCC 2 cut(s) 84, 509
BshVI ATCGAT 1 cut(s) 263
BsiHKAI GWGCWC 1 cut(s) 285
BsiSI CCGG 1 cut(s) 590
BslFI GGGAC 2 cut(s) 51, 351
BslI CCNNNNNNNGG 2 cut(s) 505, 629
BsmFI GGGAC 2 cut(s) 51, 351
BsnI GGCC 2 cut(s) 84, 509
Bsp1286I GDGCHC 3 cut(s) 285, 576, 639
Bsp143I GATC 3 cut(s) 41, 227, 486
BspANI GGCC 2 cut(s) 84, 509
BspCNI CTCAG 2 cut(s) 92, 432
BspDI ATCGAT 1 cut(s) 263
BspLI GGNNCC 1 cut(s) 339
BspPI GGATC 2 cut(s) 235, 494
BsrDI GCAATG 3 cut(s) 63, 186, 255
BsrI ACTGG 1 cut(s) 641
BssMI GATC 3 cut(s) 41, 227, 486
Bst4CI ACNGT 2 cut(s) 475, 554
BstDEI CTNAG 3 cut(s) 79, 419, 542
BstF5I GGATG 1 cut(s) 516
BstKTI GATC 3 cut(s) 44, 230, 489
BstMBI GATC 3 cut(s) 41, 227, 486
BstMWI GCNNNNNNNGC 2 cut(s) 74, 643
BstSCI CCNGG 1 cut(s) 588
BstV1I GCAGC 1 cut(s) 605
BstX2I RGATCY 2 cut(s) 227, 486
BstXI CCANNNNNNTGG 1 cut(s) 334
BstYI RGATCY 2 cut(s) 227, 486
Bsu15I ATCGAT 1 cut(s) 263
Bsu36I CCTNAGG 1 cut(s) 79
BsuRI GGCC 2 cut(s) 84, 509
BsuTUI ATCGAT 1 cut(s) 263
BtsCI GGATG 1 cut(s) 516
BtsIMutI CAGTG 2 cut(s) 559, 648
Cfr13I GGNCC 2 cut(s) 9, 338
ClaI ATCGAT 1 cut(s) 263
CviAII CATG 3 cut(s) 127, 375, 505
DdeI CTNAG 3 cut(s) 79, 419, 542
DpnI GATC 3 cut(s) 43, 229, 488
DpnII GATC 3 cut(s) 41, 227, 486
DriI GACNNNNNGTC 1 cut(s) 122
EaeI YGGCCR 1 cut(s) 507
Eam1105I GACNNNNNGTC 1 cut(s) 122
Ecl136II GAGCTC 1 cut(s) 283
Eco24I GRGCYC 3 cut(s) 285, 576, 639
Eco47I GGWCC 2 cut(s) 9, 338
Eco53kI GAGCTC 1 cut(s) 283
Eco57I CTGAAG 1 cut(s) 216
Eco81I CCTNAGG 1 cut(s) 79
EcoICRI GAGCTC 1 cut(s) 283
EcoO109I RGGNCCY 1 cut(s) 338
EcoRI GAATTC 1 cut(s) 349
EcoT38I GRGCYC 3 cut(s) 285, 576, 639
FaeI CATG 3 cut(s) 130, 378, 508
FaqI GGGAC 2 cut(s) 51, 351
FatI CATG 3 cut(s) 126, 374, 504
FblI GTMKAC 1 cut(s) 457
Fnu4HI GCNGC 1 cut(s) 594
FokI GGATG 1 cut(s) 503
FriOI GRGCYC 3 cut(s) 285, 576, 639
Fsp4HI GCNGC 1 cut(s) 594
FspBI CTAG 2 cut(s) 165, 383
GluI GCNGC 1 cut(s) 594
HaeIII GGCC 2 cut(s) 84, 509
HapII CCGG 1 cut(s) 590
Hin1II CATG 3 cut(s) 130, 378, 508
HinfI GANTC 2 cut(s) 379, 393
HpaII CCGG 1 cut(s) 590
HphI GGTGA 1 cut(s) 644
Hpy166II GTNNAC 1 cut(s) 458
Hpy188I TCNGA 1 cut(s) 653
Hpy188III TCNNGA 2 cut(s) 139, 234
Hpy8I GTNNAC 1 cut(s) 458
Hpy99I CGWCG 1 cut(s) 227
HpyAV CCTTC 1 cut(s) 237
HpyCH4III ACNGT 2 cut(s) 475, 554
HpyCH4V TGCA 6 cut(s) 68, 130, 186, 260, 470, 524
HpyF10VI GCNNNNNNNGC 2 cut(s) 74, 643
HpyF3I CTNAG 3 cut(s) 79, 419, 542
Hsp92II CATG 3 cut(s) 130, 378, 508
Kzo9I GATC 3 cut(s) 41, 227, 486
LmnI GCTCC 3 cut(s) 95, 236, 288
LpnPI CCDG 9 cut(s) 66, 87, 99, 116, 219, 225, 411, 603, 654
Lsp1109I GCAGC 1 cut(s) 605
LweI GCATC 2 cut(s) 269, 411
MaeI CTAG 2 cut(s) 165, 383
MaeIII GTNAC 2 cut(s) 367, 559
MalI GATC 3 cut(s) 43, 229, 488
MboI GATC 3 cut(s) 41, 227, 486
MboII GAAGA 1 cut(s) 222
MflI RGATCY 2 cut(s) 227, 486
MhlI GDGCHC 3 cut(s) 285, 576, 639
MlsI TGGCCA 1 cut(s) 509
MluCI AATT 4 cut(s) 119, 297, 311, 349
MluNI TGGCCA 1 cut(s) 509
MnlI CCTC 7 cut(s) 88, 174, 215, 317, 351, 428, 649
Mox20I TGGCCA 1 cut(s) 509
MscI TGGCCA 1 cut(s) 509
MseI TTAA 3 cut(s) 147, 173, 296
MslI CAYNNNNRTG 2 cut(s) 149, 332
Msp20I TGGCCA 1 cut(s) 509
MspI CCGG 1 cut(s) 590
MspR9I CCNGG 1 cut(s) 590
MwoI GCNNNNNNNGC 2 cut(s) 74, 643
NciI CCSGG 1 cut(s) 590
NdeII GATC 3 cut(s) 41, 227, 486
NlaIII CATG 3 cut(s) 130, 378, 508
NlaIV GGNNCC 1 cut(s) 339
NmuCI GTSAC 1 cut(s) 367
PfeI GAWTC 2 cut(s) 379, 393
PkrI GCNGC 1 cut(s) 595
PpuMI RGGWCCY 1 cut(s) 338
Psp124BI GAGCTC 1 cut(s) 285
Psp5II RGGWCCY 1 cut(s) 338
PspN4I GGNNCC 1 cut(s) 339
PspPI GGNCC 2 cut(s) 9, 338
PspPPI RGGWCCY 1 cut(s) 338
PsuI RGATCY 2 cut(s) 227, 486
RseI CAYNNNNRTG 2 cut(s) 149, 332
SacI GAGCTC 1 cut(s) 285
SaqAI TTAA 3 cut(s) 147, 173, 296
SatI GCNGC 1 cut(s) 594
Sau3AI GATC 3 cut(s) 41, 227, 486
Sau96I GGNCC 2 cut(s) 9, 338
ScrFI CCNGG 1 cut(s) 590
SduI GDGCHC 3 cut(s) 285, 576, 639
SetI ASST 7 cut(s) 92, 185, 285, 343, 420, 537, 676
SfaNI GCATC 2 cut(s) 269, 411
SinI GGWCC 2 cut(s) 9, 338
SmiMI CAYNNNNRTG 2 cut(s) 149, 332
Sse9I AATT 4 cut(s) 119, 297, 311, 349
SspMI CTAG 2 cut(s) 165, 383
SstI GAGCTC 1 cut(s) 285
StyD4I CCNGG 1 cut(s) 588
TaaI ACNGT 2 cut(s) 475, 554
TaqI TCGA 3 cut(s) 222, 263, 279
TasI AATT 4 cut(s) 119, 297, 311, 349
TfiI GAWTC 2 cut(s) 379, 393
Tru1I TTAA 3 cut(s) 147, 173, 296
Tru9I TTAA 3 cut(s) 147, 173, 296
TscAI CASTG 2 cut(s) 559, 648
TseFI GTSAC 1 cut(s) 367
TseI GCWGC 1 cut(s) 593
Tsp45I GTSAC 1 cut(s) 367
TspDTI ATGAA 3 cut(s) 167, 469, 493
TspGWI ACGGA 1 cut(s) 240
TspRI CASTG 2 cut(s) 559, 648
VpaK11BI GGWCC 2 cut(s) 9, 338
XapI RAATTY 2 cut(s) 311, 349
XcmI CCANNNNNNNNNTGG 1 cut(s) 611
XmiI GTMKAC 1 cut(s) 457
XspI CTAG 2 cut(s) 165, 383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.