pycom15g33160
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
N/A
Physical Location & Seq
Forward (+)
32494807 .. 32500444
5638 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1074 bp
ATGGCCACTCCAGTCGTTGAAACAGAAATCCAACCCAAAATTCCTCTTGATCCCCAAACAAACGAACAGGATGAAAGTTACTTCGCCGCTGTAGAGTTGACGGTTTCTTCCGTGCTGCCCTTGACCATGAAGGCAGTAGTTGAGCTTGACGTTTTTAATATCATAGCCAGAGAGGGTCCAAATGGCAAGCTCTCTGCATCAGAGATTGCAGCCATTATTGGTACAAAGAACCCTGATGGACCCGATATGCTGGATCGCATCCTGAGGCTCCTCGCCAGCCACTCGATCCTCAGCTGCTCAGTCGTTGGTGATGATGGCGATCACAAGTCTTTGAAGAGGCTCTACAGCCTGACTCCTGTGTCCAAGCACTATGTGACTAATGAGGCTGGTGTTTCTTTTGGCCCTGTGCTGCTGATGCTTCAAGACAAGATCTTCACAGAGAGCTGGTACCAGATGAAAAATGCAATTATCGAAGGAGGAATTCCATTTGAAATGGCTCATGGCTCGCACGCCTTCAAGTATGCAGGATACGACCAGCGGTTTAATCAAGCTTTCAACACAGGAATGAACAACACCACCACCATGCTTGTAAAGAAGATTCTTGAGGGTTACAAGGGTTTTGAGAACCTTAAGAAAGTTGTGGATGTTGGTGGTGGTCTCGGGGTGGCCATTAGTCTAATCACTGCCACATATCCTCACATTAAGGGTATCAATTATGATTTGCCTCATGTTGTAAAAGATGCTCCTAAATATCCTGGTGTGGAACATGTAGGAGGAGACATGTTTGCAAGTATTCCCGATGCCGATGCTGTTTTTATGAAGGGCATACTTCACGATTGGTTTGATGAGGACTGCATAAAATTGTTGAAAAATTGTTACAAAGCTCTTCCAATGGATGGAAAAGTGATCGTTGTGGACGCAGTTCTGCCAAACATCCCAAATAGTAGTAGTATTACGAAAAGTAACTTCCACCTCGATGTACATATGATGTCCAAACCCCGGGAGGGAAGCAGAGGAGCGCAGAAGAGTTCATGGACTTGGCAACTGCTGCTGGTTTTAGTGGCATCAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01058 ko01100 ko01110 map00940 map01058 map01100 map01110
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

358

Amino Acids

39.12

Weight (kDa)

5.88

Isoelectric Point (pI)

27.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dimerisation PF08100 32 - 126 4.1e-16 O-methyltransferase dimerisation domain
Methyltransf_2 PF00891 149 - 337 1e-56 O-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 358
Acc65I GGTACC 1 cut(s) 447
AccB1I GGYRCC 1 cut(s) 447
AccB7I CCANNNNNTGG 1 cut(s) 896
AciI CCGC 2 cut(s) 87, 538
AclWI GGATC 3 cut(s) 44, 261, 280
AcoI YGGCCR 2 cut(s) 3, 666
AcsI RAATTY 2 cut(s) 39, 480
AdeI CACNNNGTG 1 cut(s) 373
AfaI GTAC 3 cut(s) 223, 449, 981
AfiI CCNNNNNNNGG 3 cut(s) 896, 999, 1004
AflII CTTAAG 1 cut(s) 629
AflIII ACRYGT 2 cut(s) 766, 780
AgsI TTSAA 7 cut(s) 20, 334, 422, 491, 517, 556, 868
AjnI CCWGG 1 cut(s) 754
AjuI GAANNNNNNNTTGG 2 cut(s) 24, 56
AluBI AGCT 6 cut(s) 145, 190, 294, 444, 551, 884
AluI AGCT 6 cut(s) 145, 190, 294, 444, 551, 884
Alw26I GTCTC 2 cut(s) 662, 771
AlwI GGATC 3 cut(s) 44, 261, 280
Ama87I CYCGRG 2 cut(s) 659, 999
AoxI GGCC 3 cut(s) 3, 400, 666
ApeKI GCWGC 5 cut(s) 115, 209, 294, 409, 1048
ApoI RAATTY 2 cut(s) 39, 480
Asp718I GGTACC 1 cut(s) 447
AspLEI GCGC 1 cut(s) 1021
AspS9I GGNCC 3 cut(s) 176, 239, 401
AsuC2I CCSGG 2 cut(s) 1000, 1001
AsuHPI GGTGA 1 cut(s) 320
AvaI CYCGRG 2 cut(s) 659, 999
AvaII GGWCC 2 cut(s) 176, 239
AxyI CCTNAGG 1 cut(s) 263
BalI TGGCCA 2 cut(s) 5, 668
BanI GGYRCC 1 cut(s) 447
BarI GAAGNNNNNNTAC 2 cut(s) 326, 358
BbvCI CCTCAGC 1 cut(s) 290
BbvI GCAGC 5 cut(s) 102, 221, 281, 396, 1035
BccI CCATC 3 cut(s) 230, 308, 890
BcgI CGANNNNNNTGC 2 cut(s) 788, 822
BciT130I CCWGG 1 cut(s) 756
BciVI GTATCC 1 cut(s) 521
BcnI CCSGG 2 cut(s) 1000, 1001
BcoDI GTCTC 2 cut(s) 662, 771
BfmI CTRYAG 2 cut(s) 90, 343
BfrI CTTAAG 1 cut(s) 629
BfuI GTATCC 1 cut(s) 521
BglII AGATCT 1 cut(s) 429
BisI GCNGC 6 cut(s) 87, 116, 210, 295, 410, 1049
BlsI GCNGC 6 cut(s) 88, 117, 211, 296, 411, 1050
Bme1390I CCNGG 3 cut(s) 756, 1000, 1001
Bme18I GGWCC 2 cut(s) 176, 239
BmeT110I CYCGRG 2 cut(s) 659, 999
BmgT120I GGNCC 3 cut(s) 176, 239, 401
BmiI GGNNCC 4 cut(s) 177, 241, 269, 449
BmrFI CCNGG 3 cut(s) 756, 1000, 1001
BmsI GCATC 6 cut(s) 206, 267, 405, 730, 790, 796
Bpu10I CCTNAGC 1 cut(s) 290
BpuEI CTTGAG 1 cut(s) 623
BpuMI CCSGG 2 cut(s) 1000, 1001
BsaBI GATNNNNATC 1 cut(s) 318
BsaI GGTCTC 1 cut(s) 662
BsaJI CCNNGG 2 cut(s) 998, 999
Bsc4I CCNNNNNNNGG 3 cut(s) 896, 999, 1004
Bse1I ACTGG 1 cut(s) 11
Bse21I CCTNAGG 1 cut(s) 263
Bse8I GATNNNNATC 1 cut(s) 318
BseBI CCWGG 1 cut(s) 756
BseDI CCNNGG 2 cut(s) 998, 999
BseGI GGATG 5 cut(s) 76, 258, 649, 901, 933
BseJI GATNNNNATC 1 cut(s) 318
BseLI CCNNNNNNNGG 3 cut(s) 896, 999, 1004
BseMII CTCAG 3 cut(s) 254, 304, 312
BseNI ACTGG 1 cut(s) 11
BseRI GAGGAG 3 cut(s) 260, 789, 1029
BseXI GCAGC 5 cut(s) 102, 221, 281, 396, 1035
BshFI GGCC 3 cut(s) 5, 402, 668
BshNI GGYRCC 1 cut(s) 447
BsiHKCI CYCGRG 2 cut(s) 659, 999
BsiSI CCGG 1 cut(s) 1000
BslI CCNNNNNNNGG 3 cut(s) 896, 999, 1004
BsmAI GTCTC 2 cut(s) 662, 771
BsnI GGCC 3 cut(s) 5, 402, 668
Bso31I GGTCTC 1 cut(s) 662
BsoBI CYCGRG 2 cut(s) 659, 999
Bsp1407I TGTACA 1 cut(s) 979
Bsp143I GATC 6 cut(s) 49, 253, 285, 319, 429, 906
BspACI CCGC 2 cut(s) 87, 538
BspANI GGCC 3 cut(s) 5, 402, 668
BspCNI CTCAG 3 cut(s) 255, 303, 311
BspLI GGNNCC 4 cut(s) 177, 241, 269, 449
BspPI GGATC 3 cut(s) 44, 261, 280
BspQI GCTCTTC 1 cut(s) 891
BspT107I GGYRCC 1 cut(s) 447
BspTI CTTAAG 1 cut(s) 629
BspTNI GGTCTC 1 cut(s) 662
BsrGI TGTACA 1 cut(s) 979
BsrI ACTGG 1 cut(s) 11
BssECI CCNNGG 2 cut(s) 998, 999
BssMI GATC 6 cut(s) 49, 253, 285, 319, 429, 906
Bst2UI CCWGG 1 cut(s) 756
Bst4CI ACNGT 1 cut(s) 103
Bst6I CTCTTC 3 cut(s) 329, 891, 1019
BstAFI CTTAAG 1 cut(s) 629
BstAPI GCANNNNNTGC 1 cut(s) 1048
BstAUI TGTACA 1 cut(s) 979
BstC8I GCNNGC 4 cut(s) 188, 277, 506, 510
BstDEI CTNAG 3 cut(s) 263, 290, 298
BstF5I GGATG 5 cut(s) 76, 258, 649, 901, 933
BstHHI GCGC 1 cut(s) 1021
BstKTI GATC 6 cut(s) 52, 256, 288, 322, 432, 909
BstMAI GTCTC 2 cut(s) 662, 771
BstMBI GATC 6 cut(s) 49, 253, 285, 319, 429, 906
BstMWI GCNNNNNNNGC 2 cut(s) 415, 1048
BstNI CCWGG 1 cut(s) 756
BstNSI RCATGY 2 cut(s) 770, 784
BstSCI CCNGG 3 cut(s) 754, 998, 999
BstSFI CTRYAG 2 cut(s) 90, 343
BstV1I GCAGC 5 cut(s) 102, 221, 281, 396, 1035
BstX2I RGATCY 1 cut(s) 429
BstYI RGATCY 1 cut(s) 429
Bsu36I CCTNAGG 1 cut(s) 263
BsuI GTATCC 1 cut(s) 521
BsuRI GGCC 3 cut(s) 5, 402, 668
BtsCI GGATG 5 cut(s) 76, 258, 649, 901, 933
BtsI GCAGTG 1 cut(s) 681
BtsIMutI CAGTG 1 cut(s) 681
Cac8I GCNNGC 4 cut(s) 188, 277, 506, 510
CfoI GCGC 1 cut(s) 1021
Cfr13I GGNCC 3 cut(s) 176, 239, 401
Cfr9I CCCGGG 1 cut(s) 999
CseI GACGC 1 cut(s) 926
Csp6I GTAC 3 cut(s) 222, 448, 980
CspCI CAANNNNNGTGG 2 cut(s) 568, 603
CviAII CATG 7 cut(s) 127, 500, 583, 728, 767, 781, 1032
CviQI GTAC 3 cut(s) 222, 448, 980
DdeI CTNAG 3 cut(s) 263, 290, 298
DpnI GATC 6 cut(s) 51, 255, 287, 321, 431, 908
DpnII GATC 6 cut(s) 49, 253, 285, 319, 429, 906
DraIII CACNNNGTG 1 cut(s) 373
DrdI GACNNNNNNGTC 1 cut(s) 358
DseDI GACNNNNNNGTC 1 cut(s) 358
EaeI YGGCCR 2 cut(s) 3, 666
Eam1104I CTCTTC 3 cut(s) 329, 891, 1019
EarI CTCTTC 3 cut(s) 329, 891, 1019
Eco31I GGTCTC 1 cut(s) 662
Eco47I GGWCC 2 cut(s) 176, 239
Eco81I CCTNAGG 1 cut(s) 263
Eco88I CYCGRG 2 cut(s) 659, 999
EcoRI GAATTC 1 cut(s) 480
EcoRII CCWGG 1 cut(s) 754
FaeI CATG 7 cut(s) 130, 503, 586, 731, 770, 784, 1035
FatI CATG 7 cut(s) 126, 499, 582, 727, 766, 780, 1031
FauNDI CATATG 1 cut(s) 984
Fnu4HI GCNGC 6 cut(s) 87, 116, 210, 295, 410, 1049
FokI GGATG 5 cut(s) 83, 245, 656, 908, 920
Fsp4HI GCNGC 6 cut(s) 87, 116, 210, 295, 410, 1049
GlaI GCGC 1 cut(s) 1020
GluI GCNGC 6 cut(s) 87, 116, 210, 295, 410, 1049
HaeIII GGCC 3 cut(s) 5, 402, 668
HapII CCGG 1 cut(s) 1000
HgaI GACGC 1 cut(s) 926
HhaI GCGC 1 cut(s) 1021
Hin1II CATG 7 cut(s) 130, 503, 586, 731, 770, 784, 1035
Hin6I GCGC 1 cut(s) 1019
HinP1I GCGC 1 cut(s) 1019
HincII GTYRAC 1 cut(s) 99
HindII GTYRAC 1 cut(s) 99
HindIII AAGCTT 1 cut(s) 549
HinfI GANTC 2 cut(s) 352, 598
HpaII CCGG 1 cut(s) 1000
HphI GGTGA 1 cut(s) 320
Hpy166II GTNNAC 2 cut(s) 99, 916
Hpy188I TCNGA 1 cut(s) 202
Hpy188III TCNNGA 6 cut(s) 47, 262, 422, 602, 797, 833
Hpy8I GTNNAC 2 cut(s) 99, 916
HpyAV CCTTC 4 cut(s) 124, 467, 523, 814
HpyCH4III ACNGT 1 cut(s) 103
HpyCH4IV ACGT 1 cut(s) 150
HpyCH4V TGCA 6 cut(s) 197, 209, 464, 524, 788, 855
HpyF10VI GCNNNNNNNGC 2 cut(s) 415, 1048
HpyF3I CTNAG 3 cut(s) 263, 290, 298
HpySE526I ACGT 1 cut(s) 150
Hsp92II CATG 7 cut(s) 130, 503, 586, 731, 770, 784, 1035
HspAI GCGC 1 cut(s) 1019
KpnI GGTACC 1 cut(s) 451
Kzo9I GATC 6 cut(s) 49, 253, 285, 319, 429, 906
LguI GCTCTTC 1 cut(s) 891
LmnI GCTCC 3 cut(s) 273, 748, 1016
Lsp1109I GCAGC 5 cut(s) 102, 221, 281, 396, 1035
LweI GCATC 6 cut(s) 206, 267, 405, 730, 790, 796
MaeII ACGT 1 cut(s) 150
MaeIII GTNAC 5 cut(s) 77, 373, 608, 875, 962
MalI GATC 6 cut(s) 51, 255, 287, 321, 431, 908
MboI GATC 6 cut(s) 49, 253, 285, 319, 429, 906
MboII GAAGA 6 cut(s) 99, 346, 424, 607, 878, 1036
MflI RGATCY 1 cut(s) 429
MlsI TGGCCA 2 cut(s) 5, 668
MluCI AATT 7 cut(s) 39, 465, 480, 712, 860, 871, 1069
MluNI TGGCCA 2 cut(s) 5, 668
MlyI GAGTC 1 cut(s) 346
MmeI TCCRAC 1 cut(s) 55
Mox20I TGGCCA 2 cut(s) 5, 668
MscI TGGCCA 2 cut(s) 5, 668
MseI TTAA 4 cut(s) 156, 543, 630, 702
MslI CAYNNNNRTG 3 cut(s) 563, 581, 975
Msp20I TGGCCA 2 cut(s) 5, 668
MspA1I CMGCKG 3 cut(s) 89, 294, 538
MspCI CTTAAG 1 cut(s) 629
MspI CCGG 1 cut(s) 1000
MspR9I CCNGG 3 cut(s) 756, 1000, 1001
MvaI CCWGG 1 cut(s) 756
MwoI GCNNNNNNNGC 2 cut(s) 415, 1048
NciI CCSGG 2 cut(s) 1000, 1001
NdeI CATATG 1 cut(s) 984
NdeII GATC 6 cut(s) 49, 253, 285, 319, 429, 906
NlaIII CATG 7 cut(s) 130, 503, 586, 731, 770, 784, 1035
NlaIV GGNNCC 4 cut(s) 177, 241, 269, 449
NmuCI GTSAC 1 cut(s) 373
NspI RCATGY 2 cut(s) 770, 784
PciI ACATGT 2 cut(s) 766, 780
PciSI GCTCTTC 1 cut(s) 891
PfeI GAWTC 1 cut(s) 598
PflMI CCANNNNNTGG 1 cut(s) 896
PkrI GCNGC 6 cut(s) 88, 117, 211, 296, 411, 1050
PleI GAGTC 1 cut(s) 346
PpsI GAGTC 1 cut(s) 346
PscI ACATGT 2 cut(s) 766, 780
Psp6I CCWGG 1 cut(s) 754
PspGI CCWGG 1 cut(s) 754
PspN4I GGNNCC 4 cut(s) 177, 241, 269, 449
PspPI GGNCC 3 cut(s) 176, 239, 401
PsuI RGATCY 1 cut(s) 429
PvuII CAGCTG 1 cut(s) 294
RsaI GTAC 3 cut(s) 223, 449, 981
RsaNI GTAC 3 cut(s) 222, 448, 980
RseI CAYNNNNRTG 3 cut(s) 563, 581, 975
SapI GCTCTTC 1 cut(s) 891
SaqAI TTAA 4 cut(s) 156, 543, 630, 702
SatI GCNGC 6 cut(s) 87, 116, 210, 295, 410, 1049
Sau3AI GATC 6 cut(s) 49, 253, 285, 319, 429, 906
Sau96I GGNCC 3 cut(s) 176, 239, 401
SchI GAGTC 1 cut(s) 346
ScrFI CCNGG 3 cut(s) 756, 1000, 1001
SetI ASST 9 cut(s) 147, 153, 192, 296, 446, 553, 630, 886, 975
SfaNI GCATC 6 cut(s) 206, 267, 405, 730, 790, 796
SfcI CTRYAG 2 cut(s) 90, 343
SinI GGWCC 2 cut(s) 176, 239
SmaI CCCGGG 1 cut(s) 1001
SmiMI CAYNNNNRTG 3 cut(s) 563, 581, 975
SmlI CTYRAG 2 cut(s) 602, 629
SmoI CTYRAG 2 cut(s) 602, 629
Sse9I AATT 7 cut(s) 39, 465, 480, 712, 860, 871, 1069
SsiI CCGC 2 cut(s) 87, 538
StyD4I CCNGG 3 cut(s) 754, 998, 999
TaaI ACNGT 1 cut(s) 103
TaiI ACGT 1 cut(s) 153
TaqI TCGA 3 cut(s) 284, 471, 975
TasI AATT 7 cut(s) 39, 465, 480, 712, 860, 871, 1069
TatI WGTACW 1 cut(s) 979
TauI GCSGC 1 cut(s) 89
TfiI GAWTC 1 cut(s) 598
Tru1I TTAA 4 cut(s) 156, 543, 630, 702
Tru9I TTAA 4 cut(s) 156, 543, 630, 702
TscAI CASTG 1 cut(s) 688
TseFI GTSAC 1 cut(s) 373
TseI GCWGC 5 cut(s) 115, 209, 294, 409, 1048
Tsp45I GTSAC 1 cut(s) 373
TspDTI ATGAA 6 cut(s) 87, 143, 470, 581, 833, 1020
TspGWI ACGGA 1 cut(s) 100
TspMI CCCGGG 1 cut(s) 999
TspRI CASTG 1 cut(s) 688
Van91I CCANNNNNTGG 1 cut(s) 896
Vha464I CTTAAG 1 cut(s) 629
VpaK11BI GGWCC 2 cut(s) 176, 239
XapI RAATTY 2 cut(s) 39, 480
XceI RCATGY 2 cut(s) 770, 784
XmaI CCCGGG 1 cut(s) 999
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.