pycom15g33200

Monoacylglycerol lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
N/A
Physical Location & Seq
Forward (+)
32512904 .. 32513224
321 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 321 bp
ATGCCATGCTTCAGCATCACCCAATCCAAAAACTCATGCCACCGGTTCACCTTTGCCCGAGCCGGCCTCCGGTCTGCAATCACCGACCTCAAAGACGGCGCCACCACCATGCACTGCTGGGTCCCGCAATCCCCGAACCCTTCCAAGCCCAACCTCCTCCTCATCCACGGCTTCGGCGTCAACGCAATGTGGCAGTTCGTTGACCTCCTCCGCCATGTCACCCCTCACTACAACGTCTACGTGCCGGATCGATCGTCTTCTTCGGCGACTCCTACACGACCCGGCCCGACCGGTCCGAGTGGTTCCAGGCCGAGTGCGTGA

Protein Analysis

107

Amino Acids

11.57

Weight (kDa)

9.92

Isoelectric Point (pI)

70.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 98
AccI GTMKAC 1 cut(s) 237
AciI CCGC 2 cut(s) 125, 211
AclWI GGATC 1 cut(s) 255
AcyI GRCGYC 2 cut(s) 99, 177
AfiI CCNNNNNNNGG 1 cut(s) 69
AgeI ACCGGT 2 cut(s) 42, 290
AjnI CCWGG 1 cut(s) 305
AlwI GGATC 1 cut(s) 255
Ama87I CYCGRG 1 cut(s) 57
AoxI GGCC 3 cut(s) 64, 283, 308
AsiGI ACCGGT 2 cut(s) 42, 290
AspLEI GCGC 1 cut(s) 101
AspS9I GGNCC 3 cut(s) 121, 284, 293
AsuC2I CCSGG 1 cut(s) 282
AsuHPI GGTGA 4 cut(s) 10, 40, 73, 211
AvaI CYCGRG 1 cut(s) 57
AvaII GGWCC 2 cut(s) 121, 293
BanI GGYRCC 1 cut(s) 98
BbsI GAAGAC 1 cut(s) 249
BceAI ACGGC 2 cut(s) 112, 184
BciT130I CCWGG 1 cut(s) 307
BcnI CCSGG 1 cut(s) 282
BfoI RGCGCY 1 cut(s) 102
Bme1390I CCNGG 2 cut(s) 282, 307
Bme18I GGWCC 2 cut(s) 121, 293
BmeT110I CYCGRG 1 cut(s) 57
BmgT120I GGNCC 3 cut(s) 121, 284, 293
BmiI GGNNCC 4 cut(s) 100, 122, 123, 304
BmrFI CCNGG 2 cut(s) 282, 307
BmsI GCATC 1 cut(s) 24
BpiI GAAGAC 1 cut(s) 249
BplI GAGNNNNNCTC 2 cut(s) 51, 83
BpuMI CCSGG 1 cut(s) 282
Bsa29I ATCGAT 1 cut(s) 250
BsaAI YACGTR 1 cut(s) 241
BsaHI GRCGYC 2 cut(s) 99, 177
BsaJI CCNNGG 1 cut(s) 166
BsaWI WCCGGW 3 cut(s) 42, 69, 290
Bsc4I CCNNNNNNNGG 1 cut(s) 69
Bse118I RCCGGY 3 cut(s) 42, 62, 290
Bse3DI GCAATG 1 cut(s) 192
BseBI CCWGG 1 cut(s) 307
BseCI ATCGAT 1 cut(s) 250
BseDI CCNNGG 1 cut(s) 166
BseGI GGATG 1 cut(s) 162
BseLI CCNNNNNNNGG 1 cut(s) 69
BseMI GCAATG 1 cut(s) 192
BseRI GAGGAG 3 cut(s) 146, 149, 197
BseYI CCCAGC 1 cut(s) 117
Bsh1285I CGRYCG 2 cut(s) 254, 291
BshFI GGCC 3 cut(s) 66, 285, 310
BshNI GGYRCC 1 cut(s) 98
BshTI ACCGGT 2 cut(s) 42, 290
BshVI ATCGAT 1 cut(s) 250
BsiEI CGRYCG 2 cut(s) 254, 291
BsiHKCI CYCGRG 1 cut(s) 57
BsiSI CCGG 6 cut(s) 43, 63, 70, 245, 282, 291
BslFI GGGAC 1 cut(s) 107
BslI CCNNNNNNNGG 1 cut(s) 69
BsmFI GGGAC 1 cut(s) 107
BsnI GGCC 3 cut(s) 66, 285, 310
BsoBI CYCGRG 1 cut(s) 57
Bsp143I GATC 2 cut(s) 247, 251
BspACI CCGC 2 cut(s) 125, 211
BspANI GGCC 3 cut(s) 66, 285, 310
BspDI ATCGAT 1 cut(s) 250
BspLI GGNNCC 4 cut(s) 100, 122, 123, 304
BspPI GGATC 1 cut(s) 255
BspT107I GGYRCC 1 cut(s) 98
BsrDI GCAATG 1 cut(s) 192
BsrFI RCCGGY 3 cut(s) 42, 62, 290
BssAI RCCGGY 3 cut(s) 42, 62, 290
BssECI CCNNGG 1 cut(s) 166
BssMI GATC 2 cut(s) 247, 251
BssNI GRCGYC 2 cut(s) 99, 177
Bst2UI CCWGG 1 cut(s) 307
BstACI GRCGYC 2 cut(s) 99, 177
BstBAI YACGTR 1 cut(s) 241
BstC8I GCNNGC 1 cut(s) 64
BstDSI CCRYGG 1 cut(s) 166
BstF5I GGATG 1 cut(s) 162
BstH2I RGCGCY 1 cut(s) 102
BstHHI GCGC 1 cut(s) 101
BstKTI GATC 2 cut(s) 250, 254
BstMBI GATC 2 cut(s) 247, 251
BstMCI CGRYCG 2 cut(s) 254, 291
BstNI CCWGG 1 cut(s) 307
BstSCI CCNGG 2 cut(s) 280, 305
BstV2I GAAGAC 1 cut(s) 249
Bsu15I ATCGAT 1 cut(s) 250
BsuRI GGCC 3 cut(s) 66, 285, 310
BsuTUI ATCGAT 1 cut(s) 250
BtgI CCRYGG 1 cut(s) 166
BtsCI GGATG 1 cut(s) 162
BtsI GCAGTG 1 cut(s) 112
BtsIMutI CAGTG 1 cut(s) 112
Cac8I GCNNGC 1 cut(s) 64
CfoI GCGC 1 cut(s) 101
Cfr10I RCCGGY 3 cut(s) 42, 62, 290
Cfr13I GGNCC 3 cut(s) 121, 284, 293
ClaI ATCGAT 1 cut(s) 250
CpoI CGGWCCG 1 cut(s) 293
CseI GACGC 1 cut(s) 166
CspAI ACCGGT 2 cut(s) 42, 290
CspI CGGWCCG 1 cut(s) 293
CviAII CATG 4 cut(s) 6, 36, 109, 215
CviJI RGCY 6 cut(s) 62, 66, 148, 171, 285, 310
CviKI_1 RGCY 6 cut(s) 62, 66, 148, 171, 285, 310
DinI GGCGCC 1 cut(s) 100
DpnI GATC 2 cut(s) 249, 253
DpnII GATC 2 cut(s) 247, 251
EciI GGCGGA 1 cut(s) 200
Eco47I GGWCC 2 cut(s) 121, 293
Eco88I CYCGRG 1 cut(s) 57
EcoO109I RGGNCCY 1 cut(s) 121
EcoRII CCWGG 1 cut(s) 305
EgeI GGCGCC 1 cut(s) 100
EheI GGCGCC 1 cut(s) 100
FaeI CATG 4 cut(s) 9, 39, 112, 218
FaiI YATR 4 cut(s) 7, 37, 110, 216
FaqI GGGAC 1 cut(s) 107
FatI CATG 4 cut(s) 5, 35, 108, 214
FauI CCCGC 1 cut(s) 132
FblI GTMKAC 1 cut(s) 237
FokI GGATG 1 cut(s) 149
GlaI GCGC 1 cut(s) 100
GsaI CCCAGC 1 cut(s) 121
HaeII RGCGCY 1 cut(s) 102
HaeIII GGCC 3 cut(s) 66, 285, 310
HapII CCGG 6 cut(s) 43, 63, 70, 245, 282, 291
HgaI GACGC 1 cut(s) 166
HhaI GCGC 1 cut(s) 101
Hin1I GRCGYC 2 cut(s) 99, 177
Hin1II CATG 4 cut(s) 9, 39, 112, 218
Hin6I GCGC 1 cut(s) 99
HinP1I GCGC 1 cut(s) 99
HincII GTYRAC 2 cut(s) 181, 202
HindII GTYRAC 2 cut(s) 181, 202
HinfI GANTC 1 cut(s) 268
HpaII CCGG 6 cut(s) 43, 63, 70, 245, 282, 291
HphI GGTGA 4 cut(s) 10, 40, 73, 211
Hpy166II GTNNAC 4 cut(s) 48, 181, 202, 238
Hpy188I TCNGA 1 cut(s) 297
Hpy8I GTNNAC 4 cut(s) 48, 181, 202, 238
HpyAV CCTTC 1 cut(s) 150
HpyCH4IV ACGT 2 cut(s) 234, 240
HpyCH4V TGCA 2 cut(s) 77, 112
HpySE526I ACGT 2 cut(s) 234, 240
Hsp92I GRCGYC 2 cut(s) 99, 177
Hsp92II CATG 4 cut(s) 9, 39, 112, 218
HspAI GCGC 1 cut(s) 99
KasI GGCGCC 1 cut(s) 98
KflI GGGWCCC 1 cut(s) 121
KroI GCCGGC 1 cut(s) 62
KroNI GCCGGC 1 cut(s) 64
Kzo9I GATC 2 cut(s) 247, 251
LpnPI CCDG 8 cut(s) 56, 76, 83, 103, 258, 292, 295, 304
LweI GCATC 1 cut(s) 24
MaeII ACGT 2 cut(s) 234, 240
MaeIII GTNAC 1 cut(s) 217
MalI GATC 2 cut(s) 249, 253
MboI GATC 2 cut(s) 247, 251
MboII GAAGA 2 cut(s) 249, 252
Mly113I GGCGCC 1 cut(s) 99
MlyI GAGTC 1 cut(s) 262
MnlI CCTC 8 cut(s) 77, 98, 164, 167, 170, 215, 218, 234
MroNI GCCGGC 1 cut(s) 62
MslI CAYNNNNRTG 1 cut(s) 107
MspI CCGG 6 cut(s) 43, 63, 70, 245, 282, 291
MspR9I CCNGG 2 cut(s) 282, 307
MvaI CCWGG 1 cut(s) 307
NaeI GCCGGC 1 cut(s) 64
NarI GGCGCC 1 cut(s) 99
NciI CCSGG 1 cut(s) 282
NdeII GATC 2 cut(s) 247, 251
NgoMIV GCCGGC 1 cut(s) 62
NlaIII CATG 4 cut(s) 9, 39, 112, 218
NlaIV GGNNCC 4 cut(s) 100, 122, 123, 304
NmuCI GTSAC 1 cut(s) 217
PcsI WCGNNNNNNNCGW 1 cut(s) 174
PdiI GCCGGC 1 cut(s) 64
PinAI ACCGGT 2 cut(s) 42, 290
Ple19I CGATCG 1 cut(s) 254
PleI GAGTC 1 cut(s) 262
PluTI GGCGCC 1 cut(s) 102
PpsI GAGTC 1 cut(s) 262
Ppu21I YACGTR 1 cut(s) 241
PpuMI RGGWCCY 1 cut(s) 121
Psp5II RGGWCCY 1 cut(s) 121
Psp6I CCWGG 1 cut(s) 305
PspFI CCCAGC 1 cut(s) 117
PspGI CCWGG 1 cut(s) 305
PspN4I GGNNCC 4 cut(s) 100, 122, 123, 304
PspPI GGNCC 3 cut(s) 121, 284, 293
PspPPI RGGWCCY 1 cut(s) 121
PvuI CGATCG 1 cut(s) 254
RseI CAYNNNNRTG 1 cut(s) 107
Rsr2I CGGWCCG 1 cut(s) 293
RsrII CGGWCCG 1 cut(s) 293
Sau3AI GATC 2 cut(s) 247, 251
Sau96I GGNCC 3 cut(s) 121, 284, 293
SchI GAGTC 1 cut(s) 262
ScrFI CCNGG 2 cut(s) 282, 307
SetI ASST 6 cut(s) 53, 90, 156, 207, 237, 243
SfaNI GCATC 1 cut(s) 24
SfoI GGCGCC 1 cut(s) 100
SinI GGWCC 2 cut(s) 121, 293
SmiMI CAYNNNNRTG 1 cut(s) 107
SsiI CCGC 2 cut(s) 125, 211
SspDI GGCGCC 1 cut(s) 98
StyD4I CCNGG 2 cut(s) 280, 305
TaiI ACGT 2 cut(s) 237, 243
TaqI TCGA 1 cut(s) 250
TscAI CASTG 1 cut(s) 119
TseFI GTSAC 1 cut(s) 217
Tsp45I GTSAC 1 cut(s) 217
TspRI CASTG 1 cut(s) 119
VpaK11BI GGWCC 2 cut(s) 121, 293
XmiI GTMKAC 1 cut(s) 237
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.