pycom15g39130

Methionine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
N/A
Physical Location & Seq
Reverse (-)
38852062 .. 38853339
1278 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1116 bp
ATGGATGTTGATCAAAGCTTCTTGCGTGTACCCTCACCTGTCAAGGCTGCTATTTTCGAAAGTTTTGCTAGACAGAATATAGCTGAGTCAGAAATAGATATCACCACTAGCATCAAGCAATTCATCAAGAGTACTTATGGTTACCCAGTTGACAGCAGCAGTGAATTTATATATGCTGACAGCTCACTTGCTCTGTTTAACAAGCTTGTTCTTTGCTGCATTAAAGAAAGGGGTACATTGTGTTTTCCTGCTGGTGCAAATGGGAACTATGTTTCTGCTGCCAAATTTTTGAAAGCAAATATTGTAACTATACCTACTAAGCGCGCAGATGGCTTTAAACTGACAGACAAAGTGCTTCCTGGGGCACTAGAGACTGTGAGTAAGCCATGGGTATACATTTCTGGACCAACAATTAACCCAACTGGTTTGATATACAGCAACAAAGAGATTGAAATTTTACTCTCTGCGTGTGCTAAAGTTGGGGCTAGAGTTGTGATAGATACTTCCTTCTCAGGCTTGGAATATGACTTTGAGGGTTGGGGTGGCTGGAATCTGGTGGACAGTTTGTCAAAGCTAAATACTAGTAACCCATCTTTTTGTGTCTCTCTGCTTGGGGGATTATCTTTGAAGATGTTGAGTGGAGCACTCAAATTCGGGTTCCTTGTATTAAACCAGTCTGTTTTGGTGGATACATTTTACAGCTTTCCAGGATTGAGCAAGCCTCATAACACTGTTAAATATGCTGTTAAGAAGCTGCTGAGTCTTAGAGAGCAGAAACCGGGAGATCTGTGGGATGCTATTGCAGAACATATAAAAACTTTGAAAAGTCGATCCAAGTGCCTGAAAGAGACACTGGAGAAGTGCGGGTGGGATGTGGTTGAACCGTGTGGCGGTGTTTCCATGGTGGCCAAGCCCTCGTCCTACTTGAACAAGAGTGTTAAGGTTGATGACTCGAATATCCGGGAAGTCATTCACAAGGCTACTGGCTTATGCATCAACAGTGGAGCCTGGTCTGGAATTCCTGGCTACTGTCGGTTCACCATTGCACTCGAAGAAAGTGAATTCGAGCGCGCTTTGGATTGCATTGCTAAATTTAAGGATGCAACCAAGAACTGA

Protein Analysis

372

Amino Acids

40.54

Weight (kDa)

8.51

Isoelectric Point (pI)

37.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_1_2 PF00155 34 - 361 3.3e-17 Aminotransferase class I and II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 393
AccII CGCG 2 cut(s) 324, 1071
AciI CCGC 2 cut(s) 864, 891
AclWI GGATC 1 cut(s) 825
AcoI YGGCCR 1 cut(s) 906
AcsI RAATTY 7 cut(s) 164, 284, 453, 650, 1017, 1061, 1091
AfaI GTAC 3 cut(s) 30, 133, 235
AfiI CCNNNNNNNGG 1 cut(s) 890
AgsI TTSAA 6 cut(s) 292, 452, 628, 823, 881, 928
AhdI GACNNNNNGTC 1 cut(s) 565
AhlI ACTAGT 1 cut(s) 581
AjnI CCWGG 4 cut(s) 358, 706, 1007, 1021
AluBI AGCT 7 cut(s) 18, 83, 183, 205, 574, 702, 754
AluI AGCT 7 cut(s) 18, 83, 183, 205, 574, 702, 754
Alw21I GWGCWC 1 cut(s) 646
Alw26I GTCTC 3 cut(s) 365, 607, 842
AlwI GGATC 1 cut(s) 825
AoxI GGCC 1 cut(s) 906
ApeKI GCWGC 5 cut(s) 47, 156, 216, 278, 754
ApoI RAATTY 7 cut(s) 164, 284, 453, 650, 1017, 1061, 1091
Asp700I GAANNNNTTC 1 cut(s) 969
AspLEI GCGC 4 cut(s) 324, 326, 1071, 1073
AspS9I GGNCC 1 cut(s) 404
AsuC2I CCSGG 2 cut(s) 780, 962
AsuHPI GGTGA 3 cut(s) 27, 94, 1030
AsuII TTCGAA 1 cut(s) 57
AvaII GGWCC 1 cut(s) 404
BaeGI GKGCMC 1 cut(s) 367
BalI TGGCCA 1 cut(s) 908
Bbv12I GWGCWC 1 cut(s) 646
BbvI GCAGC 5 cut(s) 34, 168, 203, 265, 741
BccI CCATC 2 cut(s) 323, 598
BcgI CGANNNNNNTGC 4 cut(s) 47, 81, 819, 853
BciT130I CCWGG 4 cut(s) 360, 708, 1009, 1023
BciVI GTATCC 1 cut(s) 682
BclI TGATCA 1 cut(s) 10
BcnI CCSGG 2 cut(s) 780, 962
BcoDI GTCTC 3 cut(s) 365, 607, 842
BcuI ACTAGT 1 cut(s) 581
BfaI CTAG 5 cut(s) 69, 108, 368, 486, 582
BfuI GTATCC 1 cut(s) 682
BglII AGATCT 1 cut(s) 784
BisI GCNGC 5 cut(s) 48, 157, 217, 279, 755
BlsI GCNGC 5 cut(s) 49, 158, 218, 280, 756
BmcAI AGTACT 1 cut(s) 133
Bme1390I CCNGG 6 cut(s) 360, 708, 780, 962, 1009, 1023
Bme18I GGWCC 1 cut(s) 404
BmeRI GACNNNNNGTC 1 cut(s) 565
BmgT120I GGNCC 1 cut(s) 404
BmiI GGNNCC 2 cut(s) 659, 1006
BmrFI CCNGG 6 cut(s) 360, 708, 780, 962, 1009, 1023
BmrI ACTGGG 1 cut(s) 140
BmsI GCATC 4 cut(s) 120, 784, 1002, 1090
BmuI ACTGGG 1 cut(s) 140
BplI GAGNNNNNCTC 2 cut(s) 706, 738
BpmI CTGGAG 1 cut(s) 875
Bpu14I TTCGAA 1 cut(s) 57
BpuMI CCSGG 2 cut(s) 780, 962
BsaBI GATNNNNATC 1 cut(s) 9
BsaJI CCNNGG 3 cut(s) 359, 386, 900
Bsc4I CCNNNNNNNGG 1 cut(s) 890
Bse1I ACTGG 5 cut(s) 146, 427, 673, 858, 988
Bse3DI GCAATG 2 cut(s) 1041, 1083
Bse8I GATNNNNATC 1 cut(s) 9
BseBI CCWGG 4 cut(s) 360, 708, 1009, 1023
BseDI CCNNGG 3 cut(s) 359, 386, 900
BseGI GGATG 4 cut(s) 10, 799, 877, 1105
BseJI GATNNNNATC 1 cut(s) 9
BseLI CCNNNNNNNGG 1 cut(s) 890
BseMI GCAATG 2 cut(s) 1041, 1083
BseMII CTCAG 3 cut(s) 75, 525, 749
BseNI ACTGG 5 cut(s) 146, 427, 673, 858, 988
BsePI GCGCGC 2 cut(s) 322, 1069
BseSI GKGCMC 1 cut(s) 367
BseXI GCAGC 5 cut(s) 34, 168, 203, 265, 741
Bsh1236I CGCG 2 cut(s) 324, 1071
BshFI GGCC 1 cut(s) 908
BsiHKAI GWGCWC 1 cut(s) 646
BsiSI CCGG 2 cut(s) 779, 961
BslI CCNNNNNNNGG 1 cut(s) 890
BsmAI GTCTC 3 cut(s) 365, 607, 842
BsnI GGCC 1 cut(s) 908
Bsp119I TTCGAA 1 cut(s) 57
Bsp1286I GDGCHC 2 cut(s) 367, 646
Bsp143I GATC 3 cut(s) 10, 784, 830
Bsp19I CCATGG 2 cut(s) 386, 900
BspACI CCGC 2 cut(s) 864, 891
BspANI GGCC 1 cut(s) 908
BspCNI CTCAG 3 cut(s) 76, 524, 750
BspFNI CGCG 2 cut(s) 324, 1071
BspLI GGNNCC 2 cut(s) 659, 1006
BspPI GGATC 1 cut(s) 825
BspT104I TTCGAA 1 cut(s) 57
BsrDI GCAATG 2 cut(s) 1041, 1083
BsrI ACTGG 5 cut(s) 146, 427, 673, 858, 988
BssECI CCNNGG 3 cut(s) 359, 386, 900
BssHII GCGCGC 2 cut(s) 322, 1069
BssMI GATC 3 cut(s) 10, 784, 830
BssNAI GTATAC 1 cut(s) 394
BssT1I CCWWGG 2 cut(s) 386, 900
Bst1107I GTATAC 1 cut(s) 394
Bst2UI CCWGG 4 cut(s) 360, 708, 1009, 1023
Bst4CI ACNGT 6 cut(s) 376, 563, 733, 885, 1001, 1031
BstBI TTCGAA 1 cut(s) 57
BstC8I GCNNGC 3 cut(s) 324, 719, 1071
BstDEI CTNAG 5 cut(s) 84, 318, 511, 758, 764
BstDSI CCRYGG 2 cut(s) 386, 900
BstEII GGTNACC 1 cut(s) 140
BstF5I GGATG 4 cut(s) 10, 799, 877, 1105
BstFNI CGCG 2 cut(s) 324, 1071
BstHHI GCGC 4 cut(s) 324, 326, 1071, 1073
BstKTI GATC 3 cut(s) 13, 787, 833
BstMAI GTCTC 3 cut(s) 365, 607, 842
BstMBI GATC 3 cut(s) 10, 784, 830
BstMWI GCNNNNNNNGC 1 cut(s) 330
BstNI CCWGG 4 cut(s) 360, 708, 1009, 1023
BstPI GGTNACC 1 cut(s) 140
BstSCI CCNGG 6 cut(s) 358, 706, 778, 960, 1007, 1021
BstSLI GKGCMC 1 cut(s) 367
BstUI CGCG 2 cut(s) 324, 1071
BstV1I GCAGC 5 cut(s) 34, 168, 203, 265, 741
BstX2I RGATCY 1 cut(s) 784
BstYI RGATCY 1 cut(s) 784
BstZ17I GTATAC 1 cut(s) 394
BsuI GTATCC 1 cut(s) 682
BsuRI GGCC 1 cut(s) 908
BtgI CCRYGG 2 cut(s) 386, 900
BtsCI GGATG 4 cut(s) 10, 799, 877, 1105
BtsI GCAGTG 1 cut(s) 166
BtsIMutI CAGTG 4 cut(s) 166, 729, 851, 1006
Cac8I GCNNGC 3 cut(s) 324, 719, 1071
CfoI GCGC 4 cut(s) 324, 326, 1071, 1073
Cfr13I GGNCC 1 cut(s) 404
Csp6I GTAC 3 cut(s) 29, 132, 234
CviAII CATG 2 cut(s) 387, 901
CviQI GTAC 3 cut(s) 29, 132, 234
DdeI CTNAG 5 cut(s) 84, 318, 511, 758, 764
DpnI GATC 3 cut(s) 12, 786, 832
DpnII GATC 3 cut(s) 10, 784, 830
DraI TTTAAA 1 cut(s) 337
DriI GACNNNNNGTC 1 cut(s) 565
EaeI YGGCCR 1 cut(s) 906
Eam1105I GACNNNNNGTC 1 cut(s) 565
Eco130I CCWWGG 2 cut(s) 386, 900
Eco32I GATATC 1 cut(s) 100
Eco47I GGWCC 1 cut(s) 404
Eco91I GGTNACC 1 cut(s) 140
EcoO65I GGTNACC 1 cut(s) 140
EcoRI GAATTC 2 cut(s) 1017, 1061
EcoRII CCWGG 4 cut(s) 358, 706, 1007, 1021
EcoRV GATATC 1 cut(s) 100
EcoT14I CCWWGG 2 cut(s) 386, 900
EcoT22I ATGCAT 1 cut(s) 995
ErhI CCWWGG 2 cut(s) 386, 900
FaeI CATG 2 cut(s) 390, 904
FatI CATG 2 cut(s) 386, 900
FauI CCCGC 1 cut(s) 857
FbaI TGATCA 1 cut(s) 10
FblI GTMKAC 1 cut(s) 393
Fnu4HI GCNGC 5 cut(s) 48, 157, 217, 279, 755
FokI GGATG 4 cut(s) 17, 806, 884, 1112
Fsp4HI GCNGC 5 cut(s) 48, 157, 217, 279, 755
FspBI CTAG 5 cut(s) 69, 108, 368, 486, 582
GlaI GCGC 4 cut(s) 323, 325, 1070, 1072
GluI GCNGC 5 cut(s) 48, 157, 217, 279, 755
GsuI CTGGAG 1 cut(s) 875
HaeIII GGCC 1 cut(s) 908
HapII CCGG 2 cut(s) 779, 961
HhaI GCGC 4 cut(s) 324, 326, 1071, 1073
Hin1II CATG 2 cut(s) 390, 904
Hin6I GCGC 4 cut(s) 322, 324, 1069, 1071
HinP1I GCGC 4 cut(s) 322, 324, 1069, 1071
HincII GTYRAC 1 cut(s) 151
HindII GTYRAC 1 cut(s) 151
HindIII AAGCTT 2 cut(s) 16, 203
HinfI GANTC 4 cut(s) 86, 550, 760, 950
HpaII CCGG 2 cut(s) 779, 961
HphI GGTGA 3 cut(s) 27, 94, 1030
Hpy166II GTNNAC 5 cut(s) 29, 151, 394, 559, 1038
Hpy188I TCNGA 1 cut(s) 91
Hpy188III TCNNGA 3 cut(s) 127, 402, 1014
Hpy8I GTNNAC 5 cut(s) 29, 151, 394, 559, 1038
HpyAV CCTTC 1 cut(s) 517
HpyCH4III ACNGT 6 cut(s) 376, 563, 733, 885, 1001, 1031
HpyCH4V TGCA 7 cut(s) 219, 257, 803, 993, 1046, 1083, 1103
HpyF10VI GCNNNNNNNGC 1 cut(s) 330
HpyF3I CTNAG 5 cut(s) 84, 318, 511, 758, 764
Hsp92II CATG 2 cut(s) 390, 904
HspAI GCGC 4 cut(s) 322, 324, 1069, 1071
Ksp22I TGATCA 1 cut(s) 10
Kzo9I GATC 3 cut(s) 10, 784, 830
LmnI GCTCC 2 cut(s) 641, 1004
Lsp1109I GCAGC 5 cut(s) 34, 168, 203, 265, 741
LweI GCATC 4 cut(s) 120, 784, 1002, 1090
MaeI CTAG 5 cut(s) 69, 108, 368, 486, 582
MaeIII GTNAC 3 cut(s) 140, 304, 584
MalI GATC 3 cut(s) 12, 786, 832
MboI GATC 3 cut(s) 10, 784, 830
MboII GAAGA 2 cut(s) 640, 1064
MflI RGATCY 1 cut(s) 784
MhlI GDGCHC 2 cut(s) 367, 646
MlsI TGGCCA 1 cut(s) 908
MluCI AATT 9 cut(s) 119, 164, 284, 411, 453, 650, 1017, 1061, 1091
MluNI TGGCCA 1 cut(s) 908
MlyI GAGTC 3 cut(s) 95, 769, 944
MnlI CCTC 4 cut(s) 43, 526, 732, 925
Mox20I TGGCCA 1 cut(s) 908
Mph1103I ATGCAT 1 cut(s) 995
MroXI GAANNNNTTC 1 cut(s) 969
MscI TGGCCA 1 cut(s) 908
MseI TTAA 9 cut(s) 198, 222, 336, 414, 668, 735, 747, 939, 1095
Msp20I TGGCCA 1 cut(s) 908
MspI CCGG 2 cut(s) 779, 961
MspR9I CCNGG 6 cut(s) 360, 708, 780, 962, 1009, 1023
MvaI CCWGG 4 cut(s) 360, 708, 1009, 1023
MvnI CGCG 2 cut(s) 324, 1071
MwoI GCNNNNNNNGC 1 cut(s) 330
NciI CCSGG 2 cut(s) 780, 962
NcoI CCATGG 2 cut(s) 386, 900
NdeII GATC 3 cut(s) 10, 784, 830
NlaIII CATG 2 cut(s) 390, 904
NlaIV GGNNCC 2 cut(s) 659, 1006
NsiI ATGCAT 1 cut(s) 995
NspV TTCGAA 1 cut(s) 57
PauI GCGCGC 2 cut(s) 322, 1069
PdmI GAANNNNTTC 1 cut(s) 969
PfeI GAWTC 1 cut(s) 550
PfoI TCCNGGA 2 cut(s) 706, 960
PkrI GCNGC 5 cut(s) 49, 158, 218, 280, 756
PleI GAGTC 3 cut(s) 94, 768, 944
PpsI GAGTC 3 cut(s) 94, 768, 944
Psp6I CCWGG 4 cut(s) 358, 706, 1007, 1021
PspEI GGTNACC 1 cut(s) 140
PspGI CCWGG 4 cut(s) 358, 706, 1007, 1021
PspN4I GGNNCC 2 cut(s) 659, 1006
PspPI GGNCC 1 cut(s) 404
PsuI RGATCY 1 cut(s) 784
PteI GCGCGC 2 cut(s) 322, 1069
RsaI GTAC 3 cut(s) 30, 133, 235
RsaNI GTAC 3 cut(s) 29, 132, 234
SaqAI TTAA 9 cut(s) 198, 222, 336, 414, 668, 735, 747, 939, 1095
SatI GCNGC 5 cut(s) 48, 157, 217, 279, 755
Sau3AI GATC 3 cut(s) 10, 784, 830
Sau96I GGNCC 1 cut(s) 404
ScaI AGTACT 1 cut(s) 133
SchI GAGTC 3 cut(s) 95, 769, 944
ScrFI CCNGG 6 cut(s) 360, 708, 780, 962, 1009, 1023
SduI GDGCHC 2 cut(s) 367, 646
SfaNI GCATC 4 cut(s) 120, 784, 1002, 1090
SfuI TTCGAA 1 cut(s) 57
SinI GGWCC 1 cut(s) 404
SpeI ACTAGT 1 cut(s) 581
Sse9I AATT 9 cut(s) 119, 164, 284, 411, 453, 650, 1017, 1061, 1091
SsiI CCGC 2 cut(s) 864, 891
SspI AATATT 1 cut(s) 301
SspMI CTAG 5 cut(s) 69, 108, 368, 486, 582
StyD4I CCNGG 6 cut(s) 358, 706, 778, 960, 1007, 1021
StyI CCWWGG 2 cut(s) 386, 900
TaaI ACNGT 6 cut(s) 376, 563, 733, 885, 1001, 1031
TaqI TCGA 5 cut(s) 57, 829, 953, 1050, 1065
TasI AATT 9 cut(s) 119, 164, 284, 411, 453, 650, 1017, 1061, 1091
TatI WGTACW 1 cut(s) 131
TfiI GAWTC 1 cut(s) 550
Tru1I TTAA 9 cut(s) 198, 222, 336, 414, 668, 735, 747, 939, 1095
Tru9I TTAA 9 cut(s) 198, 222, 336, 414, 668, 735, 747, 939, 1095
TscAI CASTG 4 cut(s) 166, 736, 858, 1006
TseI GCWGC 5 cut(s) 47, 156, 216, 278, 754
TspDTI ATGAA 1 cut(s) 112
TspRI CASTG 4 cut(s) 166, 736, 858, 1006
VpaK11BI GGWCC 1 cut(s) 404
XapI RAATTY 7 cut(s) 164, 284, 453, 650, 1017, 1061, 1091
XmiI GTMKAC 1 cut(s) 393
XmnI GAANNNNTTC 1 cut(s) 969
XspI CTAG 5 cut(s) 69, 108, 368, 486, 582
ZrmI AGTACT 1 cut(s) 133
Zsp2I ATGCAT 1 cut(s) 995
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.