pycom16g01770

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
N/A
Physical Location & Seq
Reverse (-)
1085241 .. 1086511
1271 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 543 bp
ATGCGCGAAGCACCCCTCATCTTGTGTGCCTGGATTTGCCTCGCTCTAAATGTGAGGTATCCGTGTTGTTTTACTTGCCAGAAGTCCGCATCTTTTTGCGTCCACGGAGCCCACATCGTTTTGGTGTGCACGAAGCCAACCTCATTTCCGTGCGCAGCAATCACCGTGATTTGTGCAAGATTATGTCCAACGGTGAGTACAAAGCTGCTCTTCATCGGGTGTTGGCAAACCCATACCGCGAACCTCGGGTGTCAGTCGCAGTGTTTTTGCACTCAAGAGGACACATATGGCCCATTGCCGGAGCTGGTCTCGCCGGTGAAACCGGCACTTTACCGTCAGTTTACTCTTGCAGAGTTCATGCAAAGGTTTCCTCCGTCCCCAAACCACTCTGACATGTCTTCATCGTCGGTTGAATCAGAAAGCTCCAAACTTTTTGGAATTTTGACAAACCTAGATTCGTTATTGTTTGCCTTATGCGTCAAGGTGCCTTTGCTCCTCTCTAACATCTTCAACTTCCACAAGTCCTTTTTTTGCAGATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

181

Amino Acids

20.02

Weight (kDa)

8.34

Isoelectric Point (pI)

44.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 154
AccB1I GGYRCC 1 cut(s) 484
AccII CGCG 2 cut(s) 6, 239
AciI CCGC 2 cut(s) 87, 237
AcsI RAATTY 1 cut(s) 438
AfaI GTAC 1 cut(s) 199
AfiI CCNNNNNNNGG 1 cut(s) 298
AflIII ACRYGT 1 cut(s) 393
AgsI TTSAA 2 cut(s) 413, 511
AjnI CCWGG 1 cut(s) 29
AluBI AGCT 3 cut(s) 205, 304, 423
AluI AGCT 3 cut(s) 205, 304, 423
Alw21I GWGCWC 1 cut(s) 131
Alw26I GTCTC 1 cut(s) 313
Alw44I GTGCAC 1 cut(s) 127
AlwNI CAGNNNCTG 1 cut(s) 540
Ama87I CYCGRG 1 cut(s) 245
AoxI GGCC 1 cut(s) 289
ApaLI GTGCAC 1 cut(s) 127
ApeKI GCWGC 2 cut(s) 155, 205
ApoI RAATTY 1 cut(s) 438
AspLEI GCGC 2 cut(s) 6, 155
AspS9I GGNCC 1 cut(s) 290
AsuHPI GGTGA 3 cut(s) 154, 205, 328
AvaI CYCGRG 1 cut(s) 245
BaeGI GKGCMC 1 cut(s) 131
BanI GGYRCC 1 cut(s) 484
BanII GRGCYC 1 cut(s) 112
BbsI GAAGAC 1 cut(s) 390
Bbv12I GWGCWC 1 cut(s) 131
BbvI GCAGC 2 cut(s) 167, 192
BciT130I CCWGG 1 cut(s) 31
BciVI GTATCC 1 cut(s) 69
BcoDI GTCTC 1 cut(s) 313
BfaI CTAG 1 cut(s) 452
BfuI GTATCC 1 cut(s) 69
BisI GCNGC 2 cut(s) 156, 206
BlsI GCNGC 2 cut(s) 157, 207
Bme1390I CCNGG 1 cut(s) 31
BmeT110I CYCGRG 1 cut(s) 245
BmgT120I GGNCC 1 cut(s) 290
BmiI GGNNCC 2 cut(s) 109, 486
BmrFI CCNGG 1 cut(s) 31
BmsI GCATC 1 cut(s) 98
BpiI GAAGAC 1 cut(s) 390
BplI GAGNNNNNCTC 2 cut(s) 293, 325
BpuEI CTTGAG 1 cut(s) 258
BsaI GGTCTC 1 cut(s) 313
BsaJI CCNNGG 2 cut(s) 103, 244
Bsc4I CCNNNNNNNGG 1 cut(s) 298
Bse118I RCCGGY 2 cut(s) 313, 322
Bse3DI GCAATG 1 cut(s) 293
BseBI CCWGG 1 cut(s) 31
BseDI CCNNGG 2 cut(s) 103, 244
BseLI CCNNNNNNNGG 1 cut(s) 298
BseMI GCAATG 1 cut(s) 293
BseRI GAGGAG 1 cut(s) 485
BseSI GKGCMC 1 cut(s) 131
BseXI GCAGC 2 cut(s) 167, 192
Bsh1236I CGCG 2 cut(s) 6, 239
BshFI GGCC 1 cut(s) 291
BshNI GGYRCC 1 cut(s) 484
BsiHKAI GWGCWC 1 cut(s) 131
BsiHKCI CYCGRG 1 cut(s) 245
BsiSI CCGG 3 cut(s) 299, 314, 323
BslFI GGGAC 1 cut(s) 361
BslI CCNNNNNNNGG 1 cut(s) 298
BsmAI GTCTC 1 cut(s) 313
BsmFI GGGAC 1 cut(s) 361
BsnI GGCC 1 cut(s) 291
Bso31I GGTCTC 1 cut(s) 313
BsoBI CYCGRG 1 cut(s) 245
Bsp1286I GDGCHC 2 cut(s) 112, 131
BspACI CCGC 2 cut(s) 87, 237
BspANI GGCC 1 cut(s) 291
BspFNI CGCG 2 cut(s) 6, 239
BspLI GGNNCC 2 cut(s) 109, 486
BspQI GCTCTTC 1 cut(s) 215
BspT107I GGYRCC 1 cut(s) 484
BspTNI GGTCTC 1 cut(s) 313
BsrDI GCAATG 1 cut(s) 293
BsrFI RCCGGY 2 cut(s) 313, 322
BssAI RCCGGY 2 cut(s) 313, 322
BssECI CCNNGG 2 cut(s) 103, 244
Bst2UI CCWGG 1 cut(s) 31
Bst4CI ACNGT 3 cut(s) 166, 193, 335
Bst6I CTCTTC 1 cut(s) 215
BstDSI CCRYGG 1 cut(s) 103
BstFNI CGCG 2 cut(s) 6, 239
BstHHI GCGC 2 cut(s) 6, 155
BstMAI GTCTC 1 cut(s) 313
BstMWI GCNNNNNNNGC 1 cut(s) 310
BstNI CCWGG 1 cut(s) 31
BstNSI RCATGY 1 cut(s) 397
BstSCI CCNGG 1 cut(s) 29
BstSLI GKGCMC 1 cut(s) 131
BstUI CGCG 2 cut(s) 6, 239
BstV1I GCAGC 2 cut(s) 167, 192
BstV2I GAAGAC 1 cut(s) 390
BsuI GTATCC 1 cut(s) 69
BsuRI GGCC 1 cut(s) 291
BtgI CCRYGG 1 cut(s) 103
BtsI GCAGTG 1 cut(s) 266
BtsIMutI CAGTG 1 cut(s) 266
CaiI CAGNNNCTG 1 cut(s) 540
CfoI GCGC 2 cut(s) 6, 155
Cfr10I RCCGGY 2 cut(s) 313, 322
Cfr13I GGNCC 1 cut(s) 290
CseI GACGC 2 cut(s) 88, 466
Csp6I GTAC 1 cut(s) 198
CviAII CATG 2 cut(s) 358, 394
CviJI RGCY 6 cut(s) 110, 136, 205, 291, 304, 423
CviKI_1 RGCY 6 cut(s) 110, 136, 205, 291, 304, 423
CviQI GTAC 1 cut(s) 198
Eam1104I CTCTTC 1 cut(s) 215
EarI CTCTTC 1 cut(s) 215
Eco24I GRGCYC 1 cut(s) 112
Eco31I GGTCTC 1 cut(s) 313
Eco88I CYCGRG 1 cut(s) 245
EcoRII CCWGG 1 cut(s) 29
EcoT38I GRGCYC 1 cut(s) 112
FaeI CATG 2 cut(s) 361, 397
FaiI YATR 7 cut(s) 184, 234, 286, 288, 359, 395, 475
FalI AAGNNNNNCTT 2 cut(s) 194, 226
FaqI GGGAC 1 cut(s) 361
FatI CATG 2 cut(s) 357, 393
FauNDI CATATG 1 cut(s) 286
Fnu4HI GCNGC 2 cut(s) 156, 206
FriOI GRGCYC 1 cut(s) 112
Fsp4HI GCNGC 2 cut(s) 156, 206
FspBI CTAG 1 cut(s) 452
FspI TGCGCA 1 cut(s) 154
GlaI GCGC 2 cut(s) 5, 154
GluI GCNGC 2 cut(s) 156, 206
HaeIII GGCC 1 cut(s) 291
HapII CCGG 3 cut(s) 299, 314, 323
HgaI GACGC 2 cut(s) 88, 466
HhaI GCGC 2 cut(s) 6, 155
Hin1II CATG 2 cut(s) 361, 397
Hin6I GCGC 2 cut(s) 4, 153
HinP1I GCGC 2 cut(s) 4, 153
HinfI GANTC 3 cut(s) 413, 455, 537
HpaII CCGG 3 cut(s) 299, 314, 323
HphI GGTGA 3 cut(s) 154, 205, 328
Hpy166II GTNNAC 3 cut(s) 103, 129, 342
Hpy188I TCNGA 3 cut(s) 391, 418, 542
Hpy188III TCNNGA 1 cut(s) 275
Hpy8I GTNNAC 3 cut(s) 103, 129, 342
Hpy99I CGWCG 1 cut(s) 409
HpyCH4III ACNGT 3 cut(s) 166, 193, 335
HpyCH4V TGCA 6 cut(s) 129, 176, 270, 350, 361, 534
HpyF10VI GCNNNNNNNGC 1 cut(s) 310
Hsp92II CATG 2 cut(s) 361, 397
HspAI GCGC 2 cut(s) 4, 153
LguI GCTCTTC 1 cut(s) 215
LmnI GCTCC 4 cut(s) 107, 301, 428, 498
LpnPI CCDG 7 cut(s) 16, 43, 92, 290, 312, 327, 336
Lsp1109I GCAGC 2 cut(s) 167, 192
LweI GCATC 1 cut(s) 98
MaeI CTAG 1 cut(s) 452
MboII GAAGA 3 cut(s) 202, 390, 499
MhlI GDGCHC 2 cut(s) 112, 131
MluCI AATT 1 cut(s) 438
MmeI TCCRAC 1 cut(s) 212
MnlI CCTC 8 cut(s) 26, 48, 50, 151, 254, 271, 381, 506
MslI CAYNNNNRTG 1 cut(s) 148
MspI CCGG 3 cut(s) 299, 314, 323
MspR9I CCNGG 1 cut(s) 31
MvaI CCWGG 1 cut(s) 31
MvnI CGCG 2 cut(s) 6, 239
MwoI GCNNNNNNNGC 1 cut(s) 310
NdeI CATATG 1 cut(s) 286
NlaIII CATG 2 cut(s) 361, 397
NlaIV GGNNCC 2 cut(s) 109, 486
NsbI TGCGCA 1 cut(s) 154
NspI RCATGY 1 cut(s) 397
PciI ACATGT 1 cut(s) 393
PciSI GCTCTTC 1 cut(s) 215
PfeI GAWTC 3 cut(s) 413, 455, 537
PkrI GCNGC 2 cut(s) 157, 207
PscI ACATGT 1 cut(s) 393
Psp6I CCWGG 1 cut(s) 29
PspGI CCWGG 1 cut(s) 29
PspN4I GGNNCC 2 cut(s) 109, 486
PspPI GGNCC 1 cut(s) 290
PstNI CAGNNNCTG 1 cut(s) 540
RsaI GTAC 1 cut(s) 199
RsaNI GTAC 1 cut(s) 198
RseI CAYNNNNRTG 1 cut(s) 148
SapI GCTCTTC 1 cut(s) 215
SatI GCNGC 2 cut(s) 156, 206
Sau96I GGNCC 1 cut(s) 290
ScrFI CCNGG 1 cut(s) 31
SduI GDGCHC 2 cut(s) 112, 131
SetI ASST 9 cut(s) 59, 143, 207, 246, 306, 368, 425, 453, 486
SfaNI GCATC 1 cut(s) 98
SgrAI CRCCGGYG 1 cut(s) 313
SmiMI CAYNNNNRTG 1 cut(s) 148
SmlI CTYRAG 1 cut(s) 273
SmoI CTYRAG 1 cut(s) 273
Sse9I AATT 1 cut(s) 438
SsiI CCGC 2 cut(s) 87, 237
SspMI CTAG 1 cut(s) 452
StyD4I CCNGG 1 cut(s) 29
TaaI ACNGT 3 cut(s) 166, 193, 335
TasI AATT 1 cut(s) 438
TatI WGTACW 1 cut(s) 197
TfiI GAWTC 3 cut(s) 413, 455, 537
TscAI CASTG 1 cut(s) 266
TseI GCWGC 2 cut(s) 155, 205
TspDTI ATGAA 3 cut(s) 202, 346, 390
TspGWI ACGGA 4 cut(s) 51, 120, 138, 363
TspRI CASTG 1 cut(s) 266
VneI GTGCAC 1 cut(s) 127
XapI RAATTY 1 cut(s) 438
XceI RCATGY 1 cut(s) 397
XspI CTAG 1 cut(s) 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.