pycom16g04010
ERF Family

eukaryotic peptide chain release factor subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
N/A
Physical Location & Seq
Reverse (-)
2465930 .. 2469166
3237 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1191 bp
ATGATGTGCTTGATGGGTTGCTCGTGTTTGTACTCGTGCTTCTATAGAACCAAATTGAGAGGACAATATTTCTTGGAGGAGAAGCCCTGCACGGATTGCTGCGTCCATTTTTGCTGCGAGGCATGTGCTTTGTGCCAAGAGTATAGACATCTTCAGAACCAGGGCTTTGACATGTCCATCGGCTGGCACGGCAACGTTGAAAGGCAGAGACGACTGGCTCCAACATCAATGCCACTGCATCCACAACTTTATGCAGCAGGCATGACGATGAAAAAGCTTCAAAATGATCTTGCGACTTCGAAGAGCAATATCGAGAGATGGAGGATCAGAAAACTGATCAAGGAATTTGAACCCGCATATGGTTTTGGCTTGATTTCTATCATCATTCCTCCCACGAAAACAATGTCCAGTGTTACTGAGTGGCTGGCGAACCATAAAATTCGAATGCTTTCAGACCTTGAAAGTGGTTCTGAATTGTTTAAAGGTATACGTATGGCTAGGTTGGATGGTAAGAATTCTTTTGAGCCTTTCATGCCCATTGACAAAAAGGGCTTTTGTTTCGTTGTTGACGTTAGCTTTCATATTCAAGTTCTTAATGACCCCGATGACAAATATGGTTTCATTATCATGGATGATGAGTGGACACTTTTTGGGAAGCTAAAGGGTAACAGAAGCCATGTGATTCGTAAAATTAGTGTTGAAGGTAGTGGGTACTACCATGTTAAGAAGGCAGAAAAACTTGCCAAAGAGTTTTTTAAGGGCCCACAAGTTAAAGGAGTAGTGTTGAATGATTTATCAGACTTAAGGTTGAGACGTTGGCTACGCAAGAAAAAGCTGATGGAAGTGGAGATTGATCATGCAAATGAGGAGGGTTTGGAGCAGGCTATTAAGTTGTCTGAACTTAAATTTTGTAGGGAGAAGAAATTGTTAGACAAATATCTTAAGGAGATTGTTGATGGACCTGAAAAGGGTACATATGGTGCAGAAGACACAATAACAGCGTTGGAGGAGCGTGCTGCTGAGACACTCATTGTATGTGATAGTTTGGACATCATTAGGTATGTGTTCAAAGACATCAATACTGATGACACTGTTGTAAAGTATTCAAAAGAGGGGGAGTTGTTCTGCCGAGTTTATGGGGGCATTGGAGCAATTATTCGAGGCCCCGACCCGCTTTGGTTGAACCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03015 map03015
Pfam Domains
Protein Families
eRF1_1 eRF1_2 eRF1_3

Protein Analysis

397

Amino Acids

45.58

Weight (kDa)

8.24

Isoelectric Point (pI)

34.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 2 - 50 6.8e-14 PLAC8 family
eRF1_3 PF03465 304 - 362 7.7e-10 eRF1 domain 3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 183
AccI GTMKAC 1 cut(s) 487
AciI CCGC 2 cut(s) 354, 1172
AclI AACGTT 1 cut(s) 195
AclWI GGATC 1 cut(s) 332
AcsI RAATTY 4 cut(s) 344, 438, 514, 905
AcuI CTGAAG 1 cut(s) 137
AfaI GTAC 3 cut(s) 32, 713, 973
AfiI CCNNNNNNNGG 3 cut(s) 183, 359, 968
AflII CTTAAG 2 cut(s) 802, 941
AflIII ACRYGT 1 cut(s) 171
AjnI CCWGG 1 cut(s) 159
AluBI AGCT 4 cut(s) 277, 576, 658, 835
AluI AGCT 4 cut(s) 277, 576, 658, 835
Alw26I GTCTC 3 cut(s) 202, 805, 1016
AlwI GGATC 1 cut(s) 332
AoxI GGCC 2 cut(s) 760, 1162
ApaI GGGCCC 1 cut(s) 764
ApeKI GCWGC 4 cut(s) 99, 114, 254, 1016
ApoI RAATTY 4 cut(s) 344, 438, 514, 905
ArsI GACNNNNNNTTYG 2 cut(s) 389, 421
Asp700I GAANNNNTTC 1 cut(s) 448
AspS9I GGNCC 4 cut(s) 760, 761, 959, 1163
AsuII TTCGAA 2 cut(s) 299, 442
AvaII GGWCC 1 cut(s) 959
BaeGI GKGCMC 1 cut(s) 764
BanII GRGCYC 1 cut(s) 764
BarI GAAGNNNNNNTAC 2 cut(s) 23, 55
BauI CACGAG 2 cut(s) 22, 34
BbsI GAAGAC 1 cut(s) 993
BbvI GCAGC 4 cut(s) 86, 101, 266, 1003
BccI CCATC 6 cut(s) 7, 185, 312, 500, 832, 950
BceAI ACGGC 1 cut(s) 205
BcgI CGANNNNNNTGC 2 cut(s) 107, 141
BciT130I CCWGG 1 cut(s) 161
BclI TGATCA 2 cut(s) 336, 853
BcoDI GTCTC 3 cut(s) 202, 805, 1016
BfaI CTAG 1 cut(s) 498
BfmI CTRYAG 1 cut(s) 43
BfrI CTTAAG 2 cut(s) 802, 941
BisI GCNGC 4 cut(s) 100, 115, 255, 1017
BlsI GCNGC 4 cut(s) 101, 116, 256, 1018
Bme1390I CCNGG 1 cut(s) 161
Bme18I GGWCC 1 cut(s) 959
BmgT120I GGNCC 4 cut(s) 760, 761, 959, 1163
BmiI GGNNCC 3 cut(s) 219, 762, 1165
BmrFI CCNGG 1 cut(s) 161
BmsI GCATC 1 cut(s) 247
BpiI GAAGAC 1 cut(s) 993
Bpu14I TTCGAA 2 cut(s) 299, 442
BsaAI YACGTR 1 cut(s) 491
BsaBI GATNNNNATC 1 cut(s) 377
BsaJI CCNNGG 1 cut(s) 160
Bsc4I CCNNNNNNNGG 3 cut(s) 183, 359, 968
Bse1I ACTGG 2 cut(s) 219, 408
Bse8I GATNNNNATC 1 cut(s) 377
BseBI CCWGG 1 cut(s) 161
BseDI CCNNGG 1 cut(s) 160
BseGI GGATG 3 cut(s) 238, 511, 637
BseJI GATNNNNATC 1 cut(s) 377
BseLI CCNNNNNNNGG 3 cut(s) 183, 359, 968
BseMII CTCAG 2 cut(s) 408, 1011
BseNI ACTGG 2 cut(s) 219, 408
BseRI GAGGAG 3 cut(s) 92, 881, 1022
BseSI GKGCMC 1 cut(s) 764
BseXI GCAGC 4 cut(s) 86, 101, 266, 1003
BsgI GTGCAG 2 cut(s) 73, 1002
BshFI GGCC 2 cut(s) 762, 1164
BslI CCNNNNNNNGG 3 cut(s) 183, 359, 968
BsmAI GTCTC 3 cut(s) 202, 805, 1016
BsmBI CGTCTC 2 cut(s) 202, 805
BsmI GAATGC 1 cut(s) 450
BsnI GGCC 2 cut(s) 762, 1164
Bsp119I TTCGAA 2 cut(s) 299, 442
Bsp120I GGGCCC 1 cut(s) 760
Bsp1286I GDGCHC 1 cut(s) 764
Bsp143I GATC 4 cut(s) 286, 324, 336, 853
BspACI CCGC 2 cut(s) 354, 1172
BspANI GGCC 2 cut(s) 762, 1164
BspCNI CTCAG 2 cut(s) 409, 1012
BspLI GGNNCC 3 cut(s) 219, 762, 1165
BspPI GGATC 1 cut(s) 332
BspQI GCTCTTC 1 cut(s) 296
BspT104I TTCGAA 2 cut(s) 299, 442
BspTI CTTAAG 2 cut(s) 802, 941
BsrI ACTGG 2 cut(s) 219, 408
BssECI CCNNGG 1 cut(s) 160
BssMI GATC 4 cut(s) 286, 324, 336, 853
BssNAI GTATAC 1 cut(s) 488
BssSI CACGAG 2 cut(s) 22, 34
Bst1107I GTATAC 1 cut(s) 488
Bst2BI CACGAG 2 cut(s) 22, 34
Bst2UI CCWGG 1 cut(s) 161
Bst4CI ACNGT 1 cut(s) 1093
Bst6I CTCTTC 1 cut(s) 296
BstAFI CTTAAG 2 cut(s) 802, 941
BstAPI GCANNNNNTGC 1 cut(s) 96
BstBAI YACGTR 1 cut(s) 491
BstBI TTCGAA 2 cut(s) 299, 442
BstC8I GCNNGC 5 cut(s) 185, 259, 426, 882, 1014
BstDEI CTNAG 2 cut(s) 417, 1020
BstF5I GGATG 3 cut(s) 238, 511, 637
BstKTI GATC 4 cut(s) 289, 327, 339, 856
BstMAI GTCTC 3 cut(s) 202, 805, 1016
BstMBI GATC 4 cut(s) 286, 324, 336, 853
BstMWI GCNNNNNNNGC 3 cut(s) 96, 189, 532
BstNI CCWGG 1 cut(s) 161
BstNSI RCATGY 2 cut(s) 126, 175
BstSCI CCNGG 1 cut(s) 159
BstSFI CTRYAG 1 cut(s) 43
BstSLI GKGCMC 1 cut(s) 764
BstSNI TACGTA 1 cut(s) 491
BstV1I GCAGC 4 cut(s) 86, 101, 266, 1003
BstV2I GAAGAC 1 cut(s) 993
BstZ17I GTATAC 1 cut(s) 488
BsuRI GGCC 2 cut(s) 762, 1164
BtsCI GGATG 3 cut(s) 238, 511, 637
BtsI GCAGTG 1 cut(s) 233
BtsIMutI CAGTG 3 cut(s) 233, 415, 1089
Cac8I GCNNGC 5 cut(s) 185, 259, 426, 882, 1014
Cfr13I GGNCC 4 cut(s) 760, 761, 959, 1163
CseI GACGC 1 cut(s) 91
Csp6I GTAC 3 cut(s) 31, 712, 972
CviAII CATG 8 cut(s) 123, 172, 262, 532, 628, 677, 719, 857
CviQI GTAC 3 cut(s) 31, 712, 972
DdeI CTNAG 2 cut(s) 417, 1020
DpnI GATC 4 cut(s) 288, 326, 338, 855
DpnII GATC 4 cut(s) 286, 324, 336, 853
DraI TTTAAA 1 cut(s) 481
Eam1104I CTCTTC 1 cut(s) 296
EarI CTCTTC 1 cut(s) 296
Eco105I TACGTA 1 cut(s) 491
Eco24I GRGCYC 1 cut(s) 764
Eco47I GGWCC 1 cut(s) 959
Eco57I CTGAAG 1 cut(s) 137
EcoO109I RGGNCCY 2 cut(s) 760, 1163
EcoRI GAATTC 1 cut(s) 514
EcoRII CCWGG 1 cut(s) 159
EcoT38I GRGCYC 1 cut(s) 764
Esp3I CGTCTC 2 cut(s) 202, 805
FaeI CATG 8 cut(s) 126, 175, 265, 535, 631, 680, 722, 860
FatI CATG 8 cut(s) 122, 171, 261, 531, 627, 676, 718, 856
FauI CCCGC 2 cut(s) 361, 1179
FauNDI CATATG 2 cut(s) 358, 976
FbaI TGATCA 2 cut(s) 336, 853
FblI GTMKAC 1 cut(s) 487
Fnu4HI GCNGC 4 cut(s) 100, 115, 255, 1017
FokI GGATG 3 cut(s) 225, 518, 644
FriOI GRGCYC 1 cut(s) 764
Fsp4HI GCNGC 4 cut(s) 100, 115, 255, 1017
FspBI CTAG 1 cut(s) 498
GluI GCNGC 4 cut(s) 100, 115, 255, 1017
HaeIII GGCC 2 cut(s) 762, 1164
HgaI GACGC 1 cut(s) 91
Hin1II CATG 8 cut(s) 126, 175, 265, 535, 631, 680, 722, 860
HincII GTYRAC 1 cut(s) 568
HindII GTYRAC 1 cut(s) 568
HindIII AAGCTT 1 cut(s) 275
HinfI GANTC 1 cut(s) 682
Hpy166II GTNNAC 3 cut(s) 488, 568, 642
Hpy188I TCNGA 6 cut(s) 156, 329, 454, 472, 799, 898
Hpy188III TCNNGA 1 cut(s) 313
Hpy8I GTNNAC 3 cut(s) 488, 568, 642
HpyAV CCTTC 2 cut(s) 695, 721
HpyCH4III ACNGT 1 cut(s) 1093
HpyCH4IV ACGT 4 cut(s) 195, 490, 570, 814
HpyCH4V TGCA 5 cut(s) 90, 238, 254, 860, 983
HpyF10VI GCNNNNNNNGC 3 cut(s) 96, 189, 532
HpyF3I CTNAG 2 cut(s) 417, 1020
HpySE526I ACGT 4 cut(s) 195, 490, 570, 814
Hsp92II CATG 8 cut(s) 126, 175, 265, 535, 631, 680, 722, 860
Ksp22I TGATCA 2 cut(s) 336, 853
Kzo9I GATC 4 cut(s) 286, 324, 336, 853
LguI GCTCTTC 1 cut(s) 296
LmnI GCTCC 4 cut(s) 223, 877, 1009, 1148
Lsp1109I GCAGC 4 cut(s) 86, 101, 266, 1003
LweI GCATC 1 cut(s) 247
MaeI CTAG 1 cut(s) 498
MaeII ACGT 4 cut(s) 195, 490, 570, 814
MaeIII GTNAC 2 cut(s) 412, 665
MalI GATC 4 cut(s) 288, 326, 338, 855
MboI GATC 4 cut(s) 286, 324, 336, 853
MboII GAAGA 4 cut(s) 143, 313, 931, 998
MhlI GDGCHC 1 cut(s) 764
MluCI AATT 9 cut(s) 53, 344, 438, 473, 514, 690, 905, 923, 1152
MmeI TCCRAC 3 cut(s) 245, 483, 984
MroXI GAANNNNTTC 1 cut(s) 448
MseI TTAA 9 cut(s) 480, 594, 723, 756, 771, 803, 888, 903, 942
MslI CAYNNNNRTG 3 cut(s) 266, 626, 861
MspCI CTTAAG 2 cut(s) 802, 941
MspR9I CCNGG 1 cut(s) 161
Mva1269I GAATGC 1 cut(s) 450
MvaI CCWGG 1 cut(s) 161
MwoI GCNNNNNNNGC 3 cut(s) 96, 189, 532
NdeI CATATG 2 cut(s) 358, 976
NdeII GATC 4 cut(s) 286, 324, 336, 853
NlaIII CATG 8 cut(s) 126, 175, 265, 535, 631, 680, 722, 860
NlaIV GGNNCC 3 cut(s) 219, 762, 1165
NmeAIII GCCGAG 1 cut(s) 1154
NspI RCATGY 2 cut(s) 126, 175
NspV TTCGAA 2 cut(s) 299, 442
PciI ACATGT 1 cut(s) 171
PciSI GCTCTTC 1 cut(s) 296
PcsI WCGNNNNNNNCGW 1 cut(s) 567
PctI GAATGC 1 cut(s) 450
PdmI GAANNNNTTC 1 cut(s) 448
PfeI GAWTC 1 cut(s) 682
PflMI CCANNNNNTGG 1 cut(s) 183
PkrI GCNGC 4 cut(s) 101, 116, 256, 1018
Ppu21I YACGTR 1 cut(s) 491
PscI ACATGT 1 cut(s) 171
Psp1406I AACGTT 1 cut(s) 195
Psp6I CCWGG 1 cut(s) 159
PspGI CCWGG 1 cut(s) 159
PspN4I GGNNCC 3 cut(s) 219, 762, 1165
PspOMI GGGCCC 1 cut(s) 760
PspPI GGNCC 4 cut(s) 760, 761, 959, 1163
RsaI GTAC 3 cut(s) 32, 713, 973
RsaNI GTAC 3 cut(s) 31, 712, 972
RseI CAYNNNNRTG 3 cut(s) 266, 626, 861
SapI GCTCTTC 1 cut(s) 296
SaqAI TTAA 9 cut(s) 480, 594, 723, 756, 771, 803, 888, 903, 942
SatI GCNGC 4 cut(s) 100, 115, 255, 1017
Sau3AI GATC 4 cut(s) 286, 324, 336, 853
Sau96I GGNCC 4 cut(s) 760, 761, 959, 1163
ScrFI CCNGG 1 cut(s) 161
SduI GDGCHC 1 cut(s) 764
SfaNI GCATC 1 cut(s) 247
SfcI CTRYAG 1 cut(s) 43
SfuI TTCGAA 2 cut(s) 299, 442
SinI GGWCC 1 cut(s) 959
SmiMI CAYNNNNRTG 3 cut(s) 266, 626, 861
SmlI CTYRAG 2 cut(s) 802, 941
SmoI CTYRAG 2 cut(s) 802, 941
SnaBI TACGTA 1 cut(s) 491
Sse9I AATT 9 cut(s) 53, 344, 438, 473, 514, 690, 905, 923, 1152
SsiI CCGC 2 cut(s) 354, 1172
SspI AATATT 1 cut(s) 68
SspMI CTAG 1 cut(s) 498
StyD4I CCNGG 1 cut(s) 159
TaaI ACNGT 1 cut(s) 1093
TaiI ACGT 4 cut(s) 198, 493, 573, 817
TaqI TCGA 4 cut(s) 299, 312, 442, 1159
TasI AATT 9 cut(s) 53, 344, 438, 473, 514, 690, 905, 923, 1152
TatI WGTACW 1 cut(s) 30
TfiI GAWTC 1 cut(s) 682
Tru1I TTAA 9 cut(s) 480, 594, 723, 756, 771, 803, 888, 903, 942
Tru9I TTAA 9 cut(s) 480, 594, 723, 756, 771, 803, 888, 903, 942
TscAI CASTG 3 cut(s) 240, 415, 1096
TseI GCWGC 4 cut(s) 99, 114, 254, 1016
TspDTI ATGAA 4 cut(s) 284, 520, 569, 610
TspGWI ACGGA 1 cut(s) 107
TspRI CASTG 3 cut(s) 240, 415, 1096
Van91I CCANNNNNTGG 1 cut(s) 183
Vha464I CTTAAG 2 cut(s) 802, 941
VpaK11BI GGWCC 1 cut(s) 959
XapI RAATTY 4 cut(s) 344, 438, 514, 905
XceI RCATGY 2 cut(s) 126, 175
XmiI GTMKAC 1 cut(s) 487
XmnI GAANNNNTTC 1 cut(s) 448
XspI CTAG 1 cut(s) 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.