pycom16g05320

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
N/A
Physical Location & Seq
Reverse (-)
3452731 .. 3453492
762 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 705 bp
ATGGATTTTTGGATTATAGCAGCAGCAGCAACAGCAGCCACTGGGGCCGGTTACGTGGCGAAGTGTTATTGGCACAAATGGTTTTCCCGAGAGAGGGAAGGTGTAGCAGATCAATCAGTTCACGATGGTGAGGAGCTGACTGAGTCTTCTGGGGAATTGGTTTCTGATTTTGTTGTGGCAGAACAAGCTGGGTTTCTTTTCCATCAAAGACTAAAGAAAAGCAGATATCTTGGAGTTAAATCAAAAGGGCTTTCTCTAAAAGCACTTAATTCTAAAGAAAGCTGCTTATTGGCTCAGGTGGTGTTGTATGGGGAAAATGTCACAGTGGAAGAGGATGTGTATAGCCCATCATGTGCATCACCTTGTATTTTGGCAATGAGGCCATTTTTGGTTAACCATGGCAGCCAAGTCAATGACGTGTGGTTGGAGAGTGAGGAAAAGGCGCGGTGGCGGCAGGGAAGGAGGAGGAGGAGATGTAGCGGCTCAACTAAACCGGTGGCTGGCAGCCCATTTGATTCACAAGGTTTTACAGTATTCTTATTAGGAATCTGTTATCAATACTTTAAAAAAGTCGTCATGTACTCTTCCCCGACGAAAAAAATGAGGAGGAGGAATGAGTCGTTAATGACTATTTTGGAGTCGTTCAAAAAATGGACTGCTGCTTTTCTTAGTTGGAATCACTATTGGAGCAATATATACTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Pectinesterase

Protein Analysis

235

Amino Acids

26.71

Weight (kDa)

9.16

Isoelectric Point (pI)

62.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 445
AciI CCGC 3 cut(s) 445, 451, 480
AfaI GTAC 1 cut(s) 581
AfiI CCNNNNNNNGG 3 cut(s) 93, 94, 500
AflIII ACRYGT 1 cut(s) 417
AgeI ACCGGT 1 cut(s) 493
AgsI TTSAA 1 cut(s) 646
AjiI CACGTC 1 cut(s) 418
AleI CACNNNNGTG 1 cut(s) 126
AluBI AGCT 3 cut(s) 136, 188, 282
AluI AGCT 3 cut(s) 136, 188, 282
AlwNI CAGNNNCTG 1 cut(s) 41
Ama87I CYCGRG 1 cut(s) 87
AoxI GGCC 2 cut(s) 45, 380
ApeKI GCWGC 8 cut(s) 20, 23, 26, 35, 282, 402, 504, 659
AsiGI ACCGGT 1 cut(s) 493
AspLEI GCGC 1 cut(s) 445
AspS9I GGNCC 1 cut(s) 45
AsuHPI GGTGA 2 cut(s) 140, 351
AvaI CYCGRG 1 cut(s) 87
BbsI GAAGAC 1 cut(s) 138
BbvI GCAGC 8 cut(s) 32, 35, 38, 47, 269, 414, 516, 646
BccI CCATC 3 cut(s) 119, 210, 355
BglI GCCNNNNNGGC 1 cut(s) 44
BmeT110I CYCGRG 1 cut(s) 87
BmgBI CACGTC 1 cut(s) 418
BmgT120I GGNCC 1 cut(s) 45
BmiI GGNNCC 1 cut(s) 46
BmrI ACTGGG 1 cut(s) 51
BmsI GCATC 1 cut(s) 365
BmuI ACTGGG 1 cut(s) 51
BpiI GAAGAC 1 cut(s) 138
Bpu10I CCTNAGC 1 cut(s) 294
BsaAI YACGTR 1 cut(s) 55
BsaJI CCNNGG 1 cut(s) 397
BsaWI WCCGGW 1 cut(s) 493
BsaXI ACNNNNNCTCC 2 cut(s) 460, 490
Bsc4I CCNNNNNNNGG 3 cut(s) 93, 94, 500
Bse118I RCCGGY 2 cut(s) 47, 493
Bse1I ACTGG 1 cut(s) 46
Bse3DI GCAATG 1 cut(s) 381
BseDI CCNNGG 1 cut(s) 397
BseGI GGATG 1 cut(s) 340
BseLI CCNNNNNNNGG 3 cut(s) 93, 94, 500
BseMI GCAATG 1 cut(s) 381
BseMII CTCAG 2 cut(s) 132, 308
BseNI ACTGG 1 cut(s) 46
BseRI GAGGAG 6 cut(s) 146, 478, 481, 484, 619, 622
BseXI GCAGC 8 cut(s) 32, 35, 38, 47, 269, 414, 516, 646
BseYI CCCAGC 1 cut(s) 188
Bsh1236I CGCG 1 cut(s) 445
BshFI GGCC 2 cut(s) 47, 382
BshTI ACCGGT 1 cut(s) 493
BsiHKCI CYCGRG 1 cut(s) 87
BsiSI CCGG 2 cut(s) 48, 494
BslI CCNNNNNNNGG 3 cut(s) 93, 94, 500
BsnI GGCC 2 cut(s) 47, 382
BsoBI CYCGRG 1 cut(s) 87
Bsp143I GATC 1 cut(s) 109
Bsp19I CCATGG 1 cut(s) 397
BspACI CCGC 3 cut(s) 445, 451, 480
BspANI GGCC 2 cut(s) 47, 382
BspCNI CTCAG 2 cut(s) 133, 307
BspFNI CGCG 1 cut(s) 445
BspLI GGNNCC 1 cut(s) 46
BsrDI GCAATG 1 cut(s) 381
BsrFI RCCGGY 2 cut(s) 47, 493
BsrI ACTGG 1 cut(s) 46
BssAI RCCGGY 2 cut(s) 47, 493
BssECI CCNNGG 1 cut(s) 397
BssMI GATC 1 cut(s) 109
BssT1I CCWWGG 1 cut(s) 397
Bst4CI ACNGT 2 cut(s) 325, 532
Bst6I CTCTTC 2 cut(s) 324, 589
BstBAI YACGTR 1 cut(s) 55
BstC8I GCNNGC 1 cut(s) 502
BstDEI CTNAG 3 cut(s) 141, 294, 668
BstDSI CCRYGG 1 cut(s) 397
BstF5I GGATG 1 cut(s) 340
BstFNI CGCG 1 cut(s) 445
BstHHI GCGC 1 cut(s) 445
BstKTI GATC 1 cut(s) 112
BstMBI GATC 1 cut(s) 109
BstMWI GCNNNNNNNGC 6 cut(s) 26, 32, 35, 44, 185, 451
BstUI CGCG 1 cut(s) 445
BstV1I GCAGC 8 cut(s) 32, 35, 38, 47, 269, 414, 516, 646
BstV2I GAAGAC 1 cut(s) 138
BsuRI GGCC 2 cut(s) 47, 382
BtgI CCRYGG 1 cut(s) 397
BtrI CACGTC 1 cut(s) 418
BtsCI GGATG 1 cut(s) 340
BtsIMutI CAGTG 2 cut(s) 39, 330
Cac8I GCNNGC 1 cut(s) 502
CaiI CAGNNNCTG 1 cut(s) 41
CfoI GCGC 1 cut(s) 445
Cfr10I RCCGGY 2 cut(s) 47, 493
Cfr13I GGNCC 1 cut(s) 45
Csp6I GTAC 1 cut(s) 580
CspAI ACCGGT 1 cut(s) 493
CviAII CATG 3 cut(s) 351, 398, 577
CviQI GTAC 1 cut(s) 580
DdeI CTNAG 3 cut(s) 141, 294, 668
DpnI GATC 1 cut(s) 111
DpnII GATC 1 cut(s) 109
DraI TTTAAA 1 cut(s) 565
Eam1104I CTCTTC 2 cut(s) 324, 589
EarI CTCTTC 2 cut(s) 324, 589
Eco130I CCWWGG 1 cut(s) 397
Eco32I GATATC 1 cut(s) 227
Eco88I CYCGRG 1 cut(s) 87
EcoRV GATATC 1 cut(s) 227
EcoT14I CCWWGG 1 cut(s) 397
ErhI CCWWGG 1 cut(s) 397
FaeI CATG 3 cut(s) 354, 401, 580
FaiI YATR 8 cut(s) 17, 309, 342, 352, 399, 578, 695, 697
FatI CATG 3 cut(s) 350, 397, 576
FokI GGATG 1 cut(s) 347
GlaI GCGC 1 cut(s) 444
GsaI CCCAGC 1 cut(s) 192
HaeIII GGCC 2 cut(s) 47, 382
HapII CCGG 2 cut(s) 48, 494
HhaI GCGC 1 cut(s) 445
Hin1II CATG 3 cut(s) 354, 401, 580
Hin6I GCGC 1 cut(s) 443
HinP1I GCGC 1 cut(s) 443
HincII GTYRAC 1 cut(s) 394
HindII GTYRAC 1 cut(s) 394
HinfI GANTC 6 cut(s) 143, 515, 546, 617, 638, 676
HpaI GTTAAC 1 cut(s) 394
HpaII CCGG 2 cut(s) 48, 494
HphI GGTGA 2 cut(s) 140, 351
Hpy166II GTNNAC 2 cut(s) 121, 394
Hpy188I TCNGA 1 cut(s) 166
Hpy188III TCNNGA 2 cut(s) 87, 122
Hpy8I GTNNAC 2 cut(s) 121, 394
Hpy99I CGWCG 1 cut(s) 595
HpyAV CCTTC 2 cut(s) 92, 453
HpyCH4III ACNGT 2 cut(s) 325, 532
HpyCH4IV ACGT 2 cut(s) 54, 417
HpyCH4V TGCA 1 cut(s) 356
HpyF10VI GCNNNNNNNGC 6 cut(s) 26, 32, 35, 44, 185, 451
HpyF3I CTNAG 3 cut(s) 141, 294, 668
HpySE526I ACGT 2 cut(s) 54, 417
Hsp92II CATG 3 cut(s) 354, 401, 580
HspAI GCGC 1 cut(s) 443
KspAI GTTAAC 1 cut(s) 394
Kzo9I GATC 1 cut(s) 109
LmnI GCTCC 2 cut(s) 133, 687
LpnPI CCDG 8 cut(s) 27, 61, 135, 174, 281, 440, 486, 507
Lsp1109I GCAGC 8 cut(s) 32, 35, 38, 47, 269, 414, 516, 646
LweI GCATC 1 cut(s) 365
MaeII ACGT 2 cut(s) 54, 417
MaeIII GTNAC 2 cut(s) 50, 319
MalI GATC 1 cut(s) 111
MboI GATC 1 cut(s) 109
MboII GAAGA 3 cut(s) 138, 341, 576
MluCI AATT 2 cut(s) 155, 268
MlyI GAGTC 3 cut(s) 152, 626, 647
MmeI TCCRAC 2 cut(s) 405, 653
MseI TTAA 5 cut(s) 237, 267, 393, 564, 623
MslI CAYNNNNRTG 1 cut(s) 126
MspI CCGG 2 cut(s) 48, 494
MvnI CGCG 1 cut(s) 445
MwoI GCNNNNNNNGC 6 cut(s) 26, 32, 35, 44, 185, 451
NcoI CCATGG 1 cut(s) 397
NdeII GATC 1 cut(s) 109
NlaIII CATG 3 cut(s) 354, 401, 580
NlaIV GGNNCC 1 cut(s) 46
NmuCI GTSAC 1 cut(s) 319
OliI CACNNNNGTG 1 cut(s) 126
PfeI GAWTC 3 cut(s) 515, 546, 676
PflFI GACNNNGTC 1 cut(s) 142
PinAI ACCGGT 1 cut(s) 493
PleI GAGTC 3 cut(s) 151, 625, 646
PpsI GAGTC 3 cut(s) 151, 625, 646
Ppu21I YACGTR 1 cut(s) 55
PspFI CCCAGC 1 cut(s) 188
PspN4I GGNNCC 1 cut(s) 46
PspPI GGNCC 1 cut(s) 45
PstNI CAGNNNCTG 1 cut(s) 41
PsyI GACNNNGTC 1 cut(s) 142
RsaI GTAC 1 cut(s) 581
RsaNI GTAC 1 cut(s) 580
RseI CAYNNNNRTG 1 cut(s) 126
SaqAI TTAA 5 cut(s) 237, 267, 393, 564, 623
Sau3AI GATC 1 cut(s) 109
Sau96I GGNCC 1 cut(s) 45
SchI GAGTC 3 cut(s) 152, 626, 647
SetI ASST 9 cut(s) 57, 103, 138, 190, 284, 300, 364, 420, 526
SfaNI GCATC 1 cut(s) 365
SmiMI CAYNNNNRTG 1 cut(s) 126
Sse9I AATT 2 cut(s) 155, 268
SsiI CCGC 3 cut(s) 445, 451, 480
StyI CCWWGG 1 cut(s) 397
TaaI ACNGT 2 cut(s) 325, 532
TaiI ACGT 2 cut(s) 57, 420
TasI AATT 2 cut(s) 155, 268
TatI WGTACW 1 cut(s) 579
TauI GCSGC 2 cut(s) 454, 483
TfiI GAWTC 3 cut(s) 515, 546, 676
Tru1I TTAA 5 cut(s) 237, 267, 393, 564, 623
Tru9I TTAA 5 cut(s) 237, 267, 393, 564, 623
TscAI CASTG 2 cut(s) 46, 330
TseFI GTSAC 1 cut(s) 319
TseI GCWGC 8 cut(s) 20, 23, 26, 35, 282, 402, 504, 659
Tsp45I GTSAC 1 cut(s) 319
TspRI CASTG 2 cut(s) 46, 330
Tth111I GACNNNGTC 1 cut(s) 142
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.