pycom16g05430

Belongs to the 14-3-3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
N/A
Physical Location & Seq
Reverse (-)
3514946 .. 3516181
1236 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 708 bp
ATGCATAGTGTTGAAGTGAGTGGAAATGATTTGCTAGCCGTATGTCAAGATGAGTTTCTCTTTGAATTGTTATGCTTACTCGATGATGCAGAAATGGTTGAGTGTATGAAGAAAGTTGCGAAACTTGACCTTGAGCTGACTGTAGAGGAGAGGAATCTTCTTTCTGTGGGATACAAAAATGTCATAGGTGCTCGAAGGGCCTCTTGGCGCATCATGTCGTCCATTGAGCAAAAGGAAGAGTCTAAGGGAAATGAGCACAATGTCAAACTGATCGATCTGCTTGTGGAAGGGAAAGGTGACTATTATCGGTACCTTGCTGAGTTCAAGACCGATCAGGACAGAAAAGAGGCAGCTGAGCAATCACTCAAGGGATATGAGGCATGTTTAATTTTACCAATTTTAACACAAATGGATGCTGCCTCAGCCACTGCAAACAAAGATCTCCCTTCAACCCACCCGATCCGTCTCGGTCTAGCCCTCAACTTCTCTGTCTTCTACTATGAGATTATGAATTCTCCCGAGAGGTATCAGTTTACGGCTTGCCATTTGGCTAAACAGGCATTTGATGAGGCAATTGCAGAGTTGGACACCTTGAGTGAGGAGTCATACAAGGACAGCACCCTTATCATGCAATTGTTGAGAGACAACCTCACTCTCTGGACCTCTGATTTGCCTGAAGACGGAGGTACAGCATACCTCCATGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04110 ko04114 ko04151 ko04390 ko04391 ko04722 ko05169 ko05203 map04011 map04110 map04114 map04151 map04390 map04391 map04722 map05169 map05203
Pfam Domains
Protein Families
14-3-3

Protein Analysis

236

Amino Acids

26.6

Weight (kDa)

4.63

Isoelectric Point (pI)

48.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
14-3-3 PF00244 30 - 92 3.6e-21 14-3-3 protein
14-3-3 PF00244 98 - 221 9.7e-45 14-3-3 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 309
AccB1I GGYRCC 1 cut(s) 309
AclWI GGATC 1 cut(s) 454
AcsI RAATTY 1 cut(s) 511
AcuI CTGAAG 1 cut(s) 696
AfaI GTAC 2 cut(s) 311, 688
AfiI CCNNNNNNNGG 1 cut(s) 680
AgsI TTSAA 4 cut(s) 14, 65, 325, 450
AjuI GAANNNNNNNTTGG 2 cut(s) 187, 219
AluBI AGCT 2 cut(s) 136, 353
AluI AGCT 2 cut(s) 136, 353
Alw21I GWGCWC 2 cut(s) 193, 258
Alw26I GTCTC 2 cut(s) 470, 636
AlwI GGATC 1 cut(s) 454
AlwNI CAGNNNCTG 1 cut(s) 428
Ama87I CYCGRG 1 cut(s) 518
AoxI GGCC 1 cut(s) 198
ApeKI GCWGC 2 cut(s) 350, 416
ApoI RAATTY 1 cut(s) 511
ArsI GACNNNNNNTTYG 2 cut(s) 652, 684
Asp718I GGTACC 1 cut(s) 309
AspLEI GCGC 1 cut(s) 210
AspS9I GGNCC 2 cut(s) 198, 660
AsuHPI GGTGA 1 cut(s) 308
AsuNHI GCTAGC 1 cut(s) 34
AvaI CYCGRG 1 cut(s) 518
AvaII GGWCC 1 cut(s) 660
BanI GGYRCC 1 cut(s) 309
BbsI GAAGAC 2 cut(s) 484, 684
Bbv12I GWGCWC 2 cut(s) 193, 258
BbvCI CCTCAGC 1 cut(s) 421
BbvI GCAGC 2 cut(s) 362, 403
BceAI ACGGC 2 cut(s) 23, 552
BciVI GTATCC 1 cut(s) 164
BcoDI GTCTC 2 cut(s) 470, 636
BfaI CTAG 2 cut(s) 35, 473
BfmI CTRYAG 1 cut(s) 141
BfuI GTATCC 1 cut(s) 164
BglII AGATCT 1 cut(s) 439
BisI GCNGC 2 cut(s) 351, 417
BlpI GCTNAGC 1 cut(s) 354
BlsI GCNGC 2 cut(s) 352, 418
Bme18I GGWCC 1 cut(s) 660
BmeT110I CYCGRG 1 cut(s) 518
BmgT120I GGNCC 2 cut(s) 198, 660
BmiI GGNNCC 1 cut(s) 311
BmsI GCATC 3 cut(s) 76, 219, 403
BmtI GCTAGC 1 cut(s) 38
BpiI GAAGAC 2 cut(s) 484, 684
BplI GAGNNNNNCTC 2 cut(s) 633, 665
Bpu10I CCTNAGC 1 cut(s) 421
Bpu1102I GCTNAGC 1 cut(s) 354
BpuEI CTTGAG 3 cut(s) 152, 350, 613
Bsa29I ATCGAT 1 cut(s) 273
Bsc4I CCNNNNNNNGG 1 cut(s) 680
BseCI ATCGAT 1 cut(s) 273
BseGI GGATG 1 cut(s) 418
BseLI CCNNNNNNNGG 1 cut(s) 680
BseMII CTCAG 3 cut(s) 309, 345, 435
BseRI GAGGAG 2 cut(s) 161, 614
BseXI GCAGC 2 cut(s) 362, 403
BshFI GGCC 1 cut(s) 200
BshNI GGYRCC 1 cut(s) 309
BshVI ATCGAT 1 cut(s) 273
BsiHKAI GWGCWC 2 cut(s) 193, 258
BsiHKCI CYCGRG 1 cut(s) 518
BslI CCNNNNNNNGG 1 cut(s) 680
BsmAI GTCTC 2 cut(s) 470, 636
BsmBI CGTCTC 1 cut(s) 470
BsnI GGCC 1 cut(s) 200
BsoBI CYCGRG 1 cut(s) 518
Bsp1286I GDGCHC 2 cut(s) 193, 258
Bsp143I GATC 5 cut(s) 270, 274, 331, 439, 459
Bsp1720I GCTNAGC 1 cut(s) 354
BspANI GGCC 1 cut(s) 200
BspCNI CTCAG 3 cut(s) 310, 346, 434
BspDI ATCGAT 1 cut(s) 273
BspLI GGNNCC 1 cut(s) 311
BspOI GCTAGC 1 cut(s) 38
BspPI GGATC 1 cut(s) 454
BspT107I GGYRCC 1 cut(s) 309
BssMI GATC 5 cut(s) 270, 274, 331, 439, 459
Bst4CI ACNGT 1 cut(s) 142
Bst6I CTCTTC 1 cut(s) 231
BstC8I GCNNGC 2 cut(s) 36, 541
BstDEI CTNAG 4 cut(s) 243, 318, 354, 421
BstF5I GGATG 1 cut(s) 418
BstHHI GCGC 1 cut(s) 210
BstKTI GATC 5 cut(s) 273, 277, 334, 442, 462
BstMAI GTCTC 2 cut(s) 470, 636
BstMBI GATC 5 cut(s) 270, 274, 331, 439, 459
BstMWI GCNNNNNNNGC 3 cut(s) 197, 422, 557
BstNSI RCATGY 1 cut(s) 384
BstSFI CTRYAG 1 cut(s) 141
BstV1I GCAGC 2 cut(s) 362, 403
BstV2I GAAGAC 2 cut(s) 484, 684
BstX2I RGATCY 1 cut(s) 439
BstYI RGATCY 1 cut(s) 439
Bsu15I ATCGAT 1 cut(s) 273
BsuI GTATCC 1 cut(s) 164
BsuRI GGCC 1 cut(s) 200
BsuTUI ATCGAT 1 cut(s) 273
BtsCI GGATG 1 cut(s) 418
BtsI GCAGTG 1 cut(s) 426
BtsIMutI CAGTG 1 cut(s) 426
Cac8I GCNNGC 2 cut(s) 36, 541
CaiI CAGNNNCTG 1 cut(s) 428
CfoI GCGC 1 cut(s) 210
Cfr13I GGNCC 2 cut(s) 198, 660
ClaI ATCGAT 1 cut(s) 273
Csp6I GTAC 2 cut(s) 310, 687
CviAII CATG 4 cut(s) 214, 381, 628, 701
CviJI RGCY 8 cut(s) 38, 136, 200, 353, 425, 476, 539, 551
CviKI_1 RGCY 8 cut(s) 38, 136, 200, 353, 425, 476, 539, 551
CviQI GTAC 2 cut(s) 310, 687
DdeI CTNAG 4 cut(s) 243, 318, 354, 421
DpnI GATC 5 cut(s) 272, 276, 333, 441, 461
DpnII GATC 5 cut(s) 270, 274, 331, 439, 459
Eam1104I CTCTTC 1 cut(s) 231
EarI CTCTTC 1 cut(s) 231
Eco47I GGWCC 1 cut(s) 660
Eco57I CTGAAG 1 cut(s) 696
Eco88I CYCGRG 1 cut(s) 518
EcoO109I RGGNCCY 1 cut(s) 198
EcoRI GAATTC 1 cut(s) 511
EcoT22I ATGCAT 1 cut(s) 6
Esp3I CGTCTC 1 cut(s) 470
FaeI CATG 4 cut(s) 217, 384, 631, 704
FalI AAGNNNNNCTT 2 cut(s) 187, 219
FatI CATG 4 cut(s) 213, 380, 627, 700
Fnu4HI GCNGC 2 cut(s) 351, 417
FokI GGATG 1 cut(s) 425
Fsp4HI GCNGC 2 cut(s) 351, 417
FspBI CTAG 2 cut(s) 35, 473
GlaI GCGC 1 cut(s) 209
GluI GCNGC 2 cut(s) 351, 417
HaeIII GGCC 1 cut(s) 200
HhaI GCGC 1 cut(s) 210
Hin1II CATG 4 cut(s) 217, 384, 631, 704
Hin6I GCGC 1 cut(s) 208
HinP1I GCGC 1 cut(s) 208
HinfI GANTC 3 cut(s) 154, 239, 602
HphI GGTGA 1 cut(s) 308
Hpy166II GTNNAC 1 cut(s) 534
Hpy188I TCNGA 1 cut(s) 667
Hpy188III TCNNGA 5 cut(s) 47, 325, 335, 518, 658
Hpy8I GTNNAC 1 cut(s) 534
HpyAV CCTTC 3 cut(s) 189, 281, 456
HpyCH4III ACNGT 1 cut(s) 142
HpyCH4V TGCA 5 cut(s) 4, 89, 431, 578, 631
HpyF10VI GCNNNNNNNGC 3 cut(s) 197, 422, 557
HpyF3I CTNAG 4 cut(s) 243, 318, 354, 421
Hsp92II CATG 4 cut(s) 217, 384, 631, 704
HspAI GCGC 1 cut(s) 208
KpnI GGTACC 1 cut(s) 313
Kzo9I GATC 5 cut(s) 270, 274, 331, 439, 459
LpnPI CCDG 4 cut(s) 320, 542, 643, 687
Lsp1109I GCAGC 2 cut(s) 362, 403
LweI GCATC 3 cut(s) 76, 219, 403
MaeI CTAG 2 cut(s) 35, 473
MaeIII GTNAC 1 cut(s) 296
MalI GATC 5 cut(s) 272, 276, 333, 441, 461
MboI GATC 5 cut(s) 270, 274, 331, 439, 459
MboII GAAGA 5 cut(s) 121, 149, 248, 484, 689
MfeI CAATTG 2 cut(s) 573, 632
MflI RGATCY 1 cut(s) 439
MhlI GDGCHC 2 cut(s) 193, 258
MluCI AATT 6 cut(s) 65, 387, 396, 511, 573, 632
MlyI GAGTC 2 cut(s) 248, 611
MmeI TCCRAC 1 cut(s) 564
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 2 cut(s) 386, 401
MspA1I CMGCKG 1 cut(s) 353
MunI CAATTG 2 cut(s) 573, 632
MwoI GCNNNNNNNGC 3 cut(s) 197, 422, 557
NdeII GATC 5 cut(s) 270, 274, 331, 439, 459
NheI GCTAGC 1 cut(s) 34
NlaIII CATG 4 cut(s) 217, 384, 631, 704
NlaIV GGNNCC 1 cut(s) 311
NmuCI GTSAC 1 cut(s) 296
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 384
PfeI GAWTC 1 cut(s) 154
PkrI GCNGC 2 cut(s) 352, 418
PleI GAGTC 2 cut(s) 247, 610
PpsI GAGTC 2 cut(s) 247, 610
PspN4I GGNNCC 1 cut(s) 311
PspPI GGNCC 2 cut(s) 198, 660
PstNI CAGNNNCTG 1 cut(s) 428
PsuI RGATCY 1 cut(s) 439
PvuII CAGCTG 1 cut(s) 353
RsaI GTAC 2 cut(s) 311, 688
RsaNI GTAC 2 cut(s) 310, 687
SaqAI TTAA 2 cut(s) 386, 401
SatI GCNGC 2 cut(s) 351, 417
Sau3AI GATC 5 cut(s) 270, 274, 331, 439, 459
Sau96I GGNCC 2 cut(s) 198, 660
SchI GAGTC 2 cut(s) 248, 611
SduI GDGCHC 2 cut(s) 193, 258
SfaNI GCATC 3 cut(s) 76, 219, 403
SfcI CTRYAG 1 cut(s) 141
SinI GGWCC 1 cut(s) 660
SmlI CTYRAG 3 cut(s) 131, 365, 592
SmoI CTYRAG 3 cut(s) 131, 365, 592
Sse9I AATT 6 cut(s) 65, 387, 396, 511, 573, 632
SspMI CTAG 2 cut(s) 35, 473
TaaI ACNGT 1 cut(s) 142
TaqI TCGA 3 cut(s) 81, 193, 273
TaqII GACCGA 2 cut(s) 344, 458
TasI AATT 6 cut(s) 65, 387, 396, 511, 573, 632
TfiI GAWTC 1 cut(s) 154
Tru1I TTAA 2 cut(s) 386, 401
Tru9I TTAA 2 cut(s) 386, 401
TscAI CASTG 1 cut(s) 433
TseFI GTSAC 1 cut(s) 296
TseI GCWGC 2 cut(s) 350, 416
Tsp45I GTSAC 1 cut(s) 296
TspDTI ATGAA 2 cut(s) 122, 524
TspGWI ACGGA 2 cut(s) 452, 696
TspRI CASTG 1 cut(s) 433
VpaK11BI GGWCC 1 cut(s) 660
XapI RAATTY 1 cut(s) 511
XceI RCATGY 1 cut(s) 384
XspI CTAG 2 cut(s) 35, 473
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.