pycom16g06600

Bacterial PH domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
N/A
Physical Location & Seq
Forward (+)
4262062 .. 4262667
606 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 555 bp
ATGGCCTCCACCCCCATTCACTCCCCCACCACCACCACCACCACCACCACCACTTCCTCCTTAGTCCACCACCACCACCACTCTCTACAACTCTCCTCTAAATTACCCTTCCCTAACTCCCTCACAAAACCCAACACCCTCAGTTCCAACAGACCAGTCACCAGACTCAACGTGTCCACCCCAACCGACAAACCCAGCAACAGCACCCCCACCTCCACCTCCAAGAAACCCACGACGGAAACCATATACTTCGACGGCGGAGCCCACTACGGCGACCTCCTGGCCAACCTTATCCTGGGCTTTACTCTTCTCTGGTTCACCAACCTGCGGTTGACGGTAATCTCGGGACTGACGGGTCAGGATAGGAGCGATTTCTCGTACAAGGTGATCAAGGATGTACAGGTGGTGCCACGTTTCATTGGCGAGTGGGGTGATATTATCATCACCCTCATTGACCTGAGGAGTGTGCACAAGTTGAGAGAGATCGCCAAGTATTGCCTCTGTTTGGCTGAAAAGCCCGCCGTATTGAAGGAAAAGGGAGCCAAAGGCTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
bPH_2

Protein Analysis

185

Amino Acids

20.26

Weight (kDa)

9.55

Isoelectric Point (pI)

34.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bPH_2 PF03703 104 - 158 4.1e-06 Bacterial PH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 354
Acc36I ACCTGC 1 cut(s) 333
AccB1I GGYRCC 1 cut(s) 406
AciI CCGC 3 cut(s) 258, 328, 519
AcoI YGGCCR 1 cut(s) 282
AfaI GTAC 2 cut(s) 380, 399
AfiI CCNNNNNNNGG 3 cut(s) 295, 327, 505
AflIII ACRYGT 1 cut(s) 171
AgsI TTSAA 1 cut(s) 529
AjnI CCWGG 2 cut(s) 279, 294
Alw21I GWGCWC 1 cut(s) 471
Alw44I GTGCAC 1 cut(s) 467
Ama87I CYCGRG 1 cut(s) 343
AoxI GGCC 2 cut(s) 3, 282
ApaLI GTGCAC 1 cut(s) 467
AsuHPI GGTGA 5 cut(s) 151, 310, 397, 436, 443
AvaI CYCGRG 1 cut(s) 343
AxyI CCTNAGG 1 cut(s) 458
BaeGI GKGCMC 1 cut(s) 471
BalI TGGCCA 1 cut(s) 284
BanI GGYRCC 1 cut(s) 406
BanII GRGCYC 1 cut(s) 265
Bbv12I GWGCWC 1 cut(s) 471
BceAI ACGGC 3 cut(s) 271, 286, 506
BciT130I CCWGG 2 cut(s) 281, 296
BclI TGATCA 1 cut(s) 387
BfuAI ACCTGC 1 cut(s) 333
Bme1390I CCNGG 2 cut(s) 281, 296
BmeT110I CYCGRG 1 cut(s) 343
BmiI GGNNCC 3 cut(s) 262, 408, 541
BmrFI CCNGG 2 cut(s) 281, 296
BsaJI CCNNGG 1 cut(s) 295
Bsc4I CCNNNNNNNGG 3 cut(s) 295, 327, 505
Bse1I ACTGG 1 cut(s) 155
Bse21I CCTNAGG 1 cut(s) 458
BseBI CCWGG 2 cut(s) 281, 296
BseDI CCNNGG 1 cut(s) 295
BseGI GGATG 1 cut(s) 400
BseLI CCNNNNNNNGG 3 cut(s) 295, 327, 505
BseMII CTCAG 2 cut(s) 154, 449
BseNI ACTGG 1 cut(s) 155
BseRI GAGGAG 2 cut(s) 85, 475
BseSI GKGCMC 1 cut(s) 471
BseYI CCCAGC 1 cut(s) 194
BshFI GGCC 2 cut(s) 5, 284
BshNI GGYRCC 1 cut(s) 406
BsiHKAI GWGCWC 1 cut(s) 471
BsiHKCI CYCGRG 1 cut(s) 343
BslFI GGGAC 1 cut(s) 360
BslI CCNNNNNNNGG 3 cut(s) 295, 327, 505
BsmFI GGGAC 1 cut(s) 360
BsnI GGCC 2 cut(s) 5, 284
BsoBI CYCGRG 1 cut(s) 343
Bsp1286I GDGCHC 2 cut(s) 265, 471
Bsp1407I TGTACA 1 cut(s) 397
Bsp143I GATC 2 cut(s) 387, 483
BspACI CCGC 3 cut(s) 258, 328, 519
BspANI GGCC 2 cut(s) 5, 284
BspCNI CTCAG 2 cut(s) 153, 450
BspLI GGNNCC 3 cut(s) 262, 408, 541
BspMI ACCTGC 1 cut(s) 333
BspT107I GGYRCC 1 cut(s) 406
BsrGI TGTACA 1 cut(s) 397
BsrI ACTGG 1 cut(s) 155
BssECI CCNNGG 1 cut(s) 295
BssMI GATC 2 cut(s) 387, 483
Bst2UI CCWGG 2 cut(s) 281, 296
Bst4CI ACNGT 1 cut(s) 337
Bst6I CTCTTC 1 cut(s) 312
BstAUI TGTACA 1 cut(s) 397
BstC8I GCNNGC 1 cut(s) 519
BstDEI CTNAG 3 cut(s) 61, 140, 458
BstF5I GGATG 1 cut(s) 400
BstKTI GATC 2 cut(s) 390, 486
BstMBI GATC 2 cut(s) 387, 483
BstNI CCWGG 2 cut(s) 281, 296
BstSCI CCNGG 2 cut(s) 279, 294
BstSLI GKGCMC 1 cut(s) 471
Bsu36I CCTNAGG 1 cut(s) 458
BsuRI GGCC 2 cut(s) 5, 284
BtsCI GGATG 1 cut(s) 400
BveI ACCTGC 1 cut(s) 333
Cac8I GCNNGC 1 cut(s) 519
Csp6I GTAC 2 cut(s) 379, 398
CviJI RGCY 8 cut(s) 5, 263, 284, 300, 509, 517, 542, 549
CviKI_1 RGCY 8 cut(s) 5, 263, 284, 300, 509, 517, 542, 549
CviQI GTAC 2 cut(s) 379, 398
DdeI CTNAG 3 cut(s) 61, 140, 458
DpnI GATC 2 cut(s) 389, 485
DpnII GATC 2 cut(s) 387, 483
DrdI GACNNNNNNGTC 1 cut(s) 354
DseDI GACNNNNNNGTC 1 cut(s) 354
EaeI YGGCCR 1 cut(s) 282
Eam1104I CTCTTC 1 cut(s) 312
EarI CTCTTC 1 cut(s) 312
EciI GGCGGA 1 cut(s) 273
Eco24I GRGCYC 1 cut(s) 265
Eco81I CCTNAGG 1 cut(s) 458
Eco88I CYCGRG 1 cut(s) 343
EcoRII CCWGG 2 cut(s) 279, 294
EcoT38I GRGCYC 1 cut(s) 265
FaiI YATR 2 cut(s) 245, 247
FaqI GGGAC 1 cut(s) 360
FauI CCCGC 1 cut(s) 526
FbaI TGATCA 1 cut(s) 387
FokI GGATG 1 cut(s) 407
FriOI GRGCYC 1 cut(s) 265
GsaI CCCAGC 1 cut(s) 198
HaeIII GGCC 2 cut(s) 5, 284
HincII GTYRAC 1 cut(s) 333
HindII GTYRAC 1 cut(s) 333
HinfI GANTC 1 cut(s) 165
HphI GGTGA 5 cut(s) 151, 310, 397, 436, 443
Hpy166II GTNNAC 5 cut(s) 67, 177, 318, 333, 469
Hpy188III TCNNGA 2 cut(s) 345, 359
Hpy8I GTNNAC 5 cut(s) 67, 177, 318, 333, 469
Hpy99I CGWCG 2 cut(s) 238, 257
HpyAV CCTTC 2 cut(s) 118, 523
HpyCH4III ACNGT 1 cut(s) 337
HpyCH4IV ACGT 2 cut(s) 171, 412
HpyCH4V TGCA 1 cut(s) 469
HpyF3I CTNAG 3 cut(s) 61, 140, 458
HpySE526I ACGT 2 cut(s) 171, 412
Ksp22I TGATCA 1 cut(s) 387
Kzo9I GATC 2 cut(s) 387, 483
LmnI GCTCC 3 cut(s) 260, 366, 539
MaeII ACGT 2 cut(s) 171, 412
MaeIII GTNAC 1 cut(s) 157
MalI GATC 2 cut(s) 389, 485
MboI GATC 2 cut(s) 387, 483
MboII GAAGA 1 cut(s) 299
MhlI GDGCHC 2 cut(s) 265, 471
MlsI TGGCCA 1 cut(s) 284
MluCI AATT 1 cut(s) 101
MluNI TGGCCA 1 cut(s) 284
MlyI GAGTC 1 cut(s) 159
MmeI TCCRAC 1 cut(s) 171
Mox20I TGGCCA 1 cut(s) 284
MscI TGGCCA 1 cut(s) 284
MseI TTAA 1 cut(s) 553
Msp20I TGGCCA 1 cut(s) 284
MspR9I CCNGG 2 cut(s) 281, 296
MvaI CCWGG 2 cut(s) 281, 296
NdeII GATC 2 cut(s) 387, 483
NlaIV GGNNCC 3 cut(s) 262, 408, 541
NmuCI GTSAC 1 cut(s) 157
PleI GAGTC 1 cut(s) 159
PpsI GAGTC 1 cut(s) 159
Psp6I CCWGG 2 cut(s) 279, 294
PspFI CCCAGC 1 cut(s) 194
PspGI CCWGG 2 cut(s) 279, 294
PspN4I GGNNCC 3 cut(s) 262, 408, 541
RsaI GTAC 2 cut(s) 380, 399
RsaNI GTAC 2 cut(s) 379, 398
SaqAI TTAA 1 cut(s) 553
Sau3AI GATC 2 cut(s) 387, 483
SchI GAGTC 1 cut(s) 159
ScrFI CCNGG 2 cut(s) 281, 296
SduI GDGCHC 2 cut(s) 265, 471
Sse9I AATT 1 cut(s) 101
SsiI CCGC 3 cut(s) 258, 328, 519
StyD4I CCNGG 2 cut(s) 279, 294
TaaI ACNGT 1 cut(s) 337
TaiI ACGT 2 cut(s) 174, 415
TaqI TCGA 1 cut(s) 252
TasI AATT 1 cut(s) 101
TatI WGTACW 1 cut(s) 397
Tru1I TTAA 1 cut(s) 553
Tru9I TTAA 1 cut(s) 553
TseFI GTSAC 1 cut(s) 157
Tsp45I GTSAC 1 cut(s) 157
TspDTI ATGAA 1 cut(s) 406
TspGWI ACGGA 1 cut(s) 251
VneI GTGCAC 1 cut(s) 467
XcmI CCANNNNNNNNNTGG 1 cut(s) 292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.