pycom16g08560

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
N/A
Physical Location & Seq
Forward (+)
5701896 .. 5702399
504 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 504 bp
ATGCGGTTCGATGGTTGCCTAAAATATCGAAAAACGAATGTGATCGATCGACAAGCAGCGTCGGATTCGGATTCAGACTCGGCCAATGATTTAACCTCAAAGCATAGGTTGATCTTGGAGTCCATCGTAAGTTTAGTTCCAGTCGAAAAAGGTGCTGCTTCCACTAGCTTTCTGCTTAAACTTTTAAAGGCAGCCAACATTCTCAATGCTTCTTCTTCTTCAAAAATGGAGTTGGCTAGAAGGGTAGGGCTTCAATTGGATAATGCAACAGTCAATGACTTGCTAATCCCCTCTGCGTCGTGTGAAAGCGACACGCGATATGATGTGGACATAGTCATGACAATTTTGGAGCACTTCTTGCAGCAGGGGCAGAGCCCCCCAACTAGTCCTCCGAGGTCCAAGCTTGGTTTCGAAAGGAGGAGGTCTCGCTCTGCGGAGAACATTGATTTTGAGTTTCAGGAGAGCCGGATCGATCTTCCTCGGCGTCACATAGCTCGAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NPH3

Protein Analysis

168

Amino Acids

18.66

Weight (kDa)

8.64

Isoelectric Point (pI)

75.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPH3 PF03000 19 - 129 1.2e-32 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 316
AciI CCGC 2 cut(s) 4, 434
AclWI GGATC 1 cut(s) 476
AcoI YGGCCR 1 cut(s) 81
AcyI GRCGYC 1 cut(s) 484
AgsI TTSAA 2 cut(s) 222, 254
AhlI ACTAGT 1 cut(s) 383
AjuI GAANNNNNNNTTGG 2 cut(s) 392, 424
AluBI AGCT 4 cut(s) 168, 403, 494, 501
AluI AGCT 4 cut(s) 168, 403, 494, 501
Alw21I GWGCWC 1 cut(s) 354
Alw26I GTCTC 1 cut(s) 429
AlwI GGATC 1 cut(s) 476
AoxI GGCC 1 cut(s) 81
ApeKI GCWGC 4 cut(s) 56, 155, 191, 361
AspS9I GGNCC 1 cut(s) 396
AsuII TTCGAA 1 cut(s) 411
AvaII GGWCC 1 cut(s) 396
BanII GRGCYC 1 cut(s) 377
Bbv12I GWGCWC 1 cut(s) 354
BbvI GCAGC 4 cut(s) 68, 142, 203, 373
BccI CCATC 2 cut(s) 5, 131
BcgI CGANNNNNNTGC 2 cut(s) 134, 168
BcoDI GTCTC 1 cut(s) 429
BcuI ACTAGT 1 cut(s) 383
BfaI CTAG 3 cut(s) 165, 237, 384
BisI GCNGC 4 cut(s) 57, 156, 192, 362
BlsI GCNGC 4 cut(s) 58, 157, 193, 363
Bme18I GGWCC 1 cut(s) 396
BmgT120I GGNCC 1 cut(s) 396
BplI GAGNNNNNCTC 2 cut(s) 409, 441
Bpu14I TTCGAA 1 cut(s) 411
Bsa29I ATCGAT 2 cut(s) 45, 471
BsaHI GRCGYC 1 cut(s) 484
BsaI GGTCTC 1 cut(s) 429
BsaJI CCNNGG 2 cut(s) 392, 479
BsaXI ACNNNNNCTCC 2 cut(s) 373, 403
Bse1I ACTGG 1 cut(s) 140
BseCI ATCGAT 2 cut(s) 45, 471
BseDI CCNNGG 2 cut(s) 392, 479
BseNI ACTGG 1 cut(s) 140
BseRI GAGGAG 1 cut(s) 433
BseXI GCAGC 4 cut(s) 68, 142, 203, 373
Bsh1236I CGCG 1 cut(s) 316
Bsh1285I CGRYCG 1 cut(s) 49
BshFI GGCC 1 cut(s) 83
BshVI ATCGAT 2 cut(s) 45, 471
BsiEI CGRYCG 1 cut(s) 49
BsiHKAI GWGCWC 1 cut(s) 354
BsiSI CCGG 1 cut(s) 466
BsmAI GTCTC 1 cut(s) 429
BsnI GGCC 1 cut(s) 83
Bso31I GGTCTC 1 cut(s) 429
Bsp119I TTCGAA 1 cut(s) 411
Bsp1286I GDGCHC 2 cut(s) 354, 377
Bsp143I GATC 5 cut(s) 42, 46, 111, 468, 472
BspACI CCGC 2 cut(s) 4, 434
BspANI GGCC 1 cut(s) 83
BspDI ATCGAT 2 cut(s) 45, 471
BspFNI CGCG 1 cut(s) 316
BspHI TCATGA 1 cut(s) 336
BspPI GGATC 1 cut(s) 476
BspT104I TTCGAA 1 cut(s) 411
BspTNI GGTCTC 1 cut(s) 429
BsrI ACTGG 1 cut(s) 140
BssECI CCNNGG 2 cut(s) 392, 479
BssMI GATC 5 cut(s) 42, 46, 111, 468, 472
BssNI GRCGYC 1 cut(s) 484
Bst4CI ACNGT 1 cut(s) 271
BstACI GRCGYC 1 cut(s) 484
BstAPI GCANNNNNTGC 1 cut(s) 358
BstBI TTCGAA 1 cut(s) 411
BstFNI CGCG 1 cut(s) 316
BstKTI GATC 5 cut(s) 45, 49, 114, 471, 475
BstMAI GTCTC 1 cut(s) 429
BstMBI GATC 5 cut(s) 42, 46, 111, 468, 472
BstMCI CGRYCG 1 cut(s) 49
BstMWI GCNNNNNNNGC 2 cut(s) 358, 367
BstUI CGCG 1 cut(s) 316
BstV1I GCAGC 4 cut(s) 68, 142, 203, 373
Bsu15I ATCGAT 2 cut(s) 45, 471
BsuRI GGCC 1 cut(s) 83
BsuTUI ATCGAT 2 cut(s) 45, 471
CciI TCATGA 1 cut(s) 336
Cfr13I GGNCC 1 cut(s) 396
ClaI ATCGAT 2 cut(s) 45, 471
CseI GACGC 3 cut(s) 48, 285, 473
CviAII CATG 1 cut(s) 337
DpnI GATC 5 cut(s) 44, 48, 113, 470, 474
DpnII GATC 5 cut(s) 42, 46, 111, 468, 472
DraI TTTAAA 1 cut(s) 186
EaeI YGGCCR 1 cut(s) 81
Eco24I GRGCYC 1 cut(s) 377
Eco31I GGTCTC 1 cut(s) 429
Eco47I GGWCC 1 cut(s) 396
EcoT38I GRGCYC 1 cut(s) 377
FaeI CATG 1 cut(s) 340
FaiI YATR 5 cut(s) 105, 321, 332, 338, 491
FatI CATG 1 cut(s) 336
Fnu4HI GCNGC 4 cut(s) 57, 156, 192, 362
FriOI GRGCYC 1 cut(s) 377
Fsp4HI GCNGC 4 cut(s) 57, 156, 192, 362
FspBI CTAG 3 cut(s) 165, 237, 384
GluI GCNGC 4 cut(s) 57, 156, 192, 362
HaeIII GGCC 1 cut(s) 83
HapII CCGG 1 cut(s) 466
HgaI GACGC 3 cut(s) 48, 285, 473
Hin1I GRCGYC 1 cut(s) 484
Hin1II CATG 1 cut(s) 340
HindIII AAGCTT 1 cut(s) 401
HinfI GANTC 4 cut(s) 65, 71, 77, 119
HpaII CCGG 1 cut(s) 466
Hpy166II GTNNAC 1 cut(s) 328
Hpy188I TCNGA 4 cut(s) 64, 70, 76, 393
Hpy188III TCNNGA 2 cut(s) 337, 458
Hpy8I GTNNAC 1 cut(s) 328
Hpy99I CGWCG 2 cut(s) 64, 301
HpyAV CCTTC 1 cut(s) 234
HpyCH4III ACNGT 1 cut(s) 271
HpyCH4V TGCA 2 cut(s) 266, 361
HpyF10VI GCNNNNNNNGC 2 cut(s) 358, 367
Hsp92I GRCGYC 1 cut(s) 484
Hsp92II CATG 1 cut(s) 340
Kzo9I GATC 5 cut(s) 42, 46, 111, 468, 472
LmnI GCTCC 1 cut(s) 349
LpnPI CCDG 4 cut(s) 153, 350, 443, 479
Lsp1109I GCAGC 4 cut(s) 68, 142, 203, 373
MaeI CTAG 3 cut(s) 165, 237, 384
MaeIII GTNAC 1 cut(s) 485
MalI GATC 5 cut(s) 44, 48, 113, 470, 474
MboI GATC 5 cut(s) 42, 46, 111, 468, 472
MboII GAAGA 4 cut(s) 204, 207, 210, 467
MfeI CAATTG 1 cut(s) 254
MhlI GDGCHC 2 cut(s) 354, 377
MluCI AATT 2 cut(s) 254, 342
MlyI GAGTC 2 cut(s) 71, 128
MmeI TCCRAC 1 cut(s) 42
MnlI CCTC 7 cut(s) 106, 301, 387, 399, 411, 414, 489
MseI TTAA 3 cut(s) 92, 177, 185
MslI CAYNNNNRTG 1 cut(s) 335
MspI CCGG 1 cut(s) 466
MunI CAATTG 1 cut(s) 254
MvnI CGCG 1 cut(s) 316
MwoI GCNNNNNNNGC 2 cut(s) 358, 367
NdeII GATC 5 cut(s) 42, 46, 111, 468, 472
NlaIII CATG 1 cut(s) 340
NmeAIII GCCGAG 2 cut(s) 59, 460
NmuCI GTSAC 1 cut(s) 485
NspV TTCGAA 1 cut(s) 411
PagI TCATGA 1 cut(s) 336
PfeI GAWTC 2 cut(s) 65, 71
PflFI GACNNNGTC 1 cut(s) 332
PkrI GCNGC 4 cut(s) 58, 157, 193, 363
Ple19I CGATCG 1 cut(s) 49
PleI GAGTC 2 cut(s) 71, 127
PpsI GAGTC 2 cut(s) 71, 127
PspPI GGNCC 1 cut(s) 396
PsrI GAACNNNNNNTAC 2 cut(s) 120, 152
PsyI GACNNNGTC 1 cut(s) 332
PvuI CGATCG 1 cut(s) 49
RseI CAYNNNNRTG 1 cut(s) 335
SaqAI TTAA 3 cut(s) 92, 177, 185
SatI GCNGC 4 cut(s) 57, 156, 192, 362
Sau3AI GATC 5 cut(s) 42, 46, 111, 468, 472
Sau96I GGNCC 1 cut(s) 396
SchI GAGTC 2 cut(s) 71, 128
SduI GDGCHC 2 cut(s) 354, 377
SetI ASST 9 cut(s) 98, 110, 154, 170, 398, 405, 425, 496, 503
SfuI TTCGAA 1 cut(s) 411
SinI GGWCC 1 cut(s) 396
SmiMI CAYNNNNRTG 1 cut(s) 335
SpeI ACTAGT 1 cut(s) 383
Sse9I AATT 2 cut(s) 254, 342
SsiI CCGC 2 cut(s) 4, 434
SspMI CTAG 3 cut(s) 165, 237, 384
TaaI ACNGT 1 cut(s) 271
TaqI TCGA 8 cut(s) 9, 28, 45, 49, 144, 411, 471, 496
TasI AATT 2 cut(s) 254, 342
TfiI GAWTC 2 cut(s) 65, 71
Tru1I TTAA 3 cut(s) 92, 177, 185
Tru9I TTAA 3 cut(s) 92, 177, 185
TseFI GTSAC 1 cut(s) 485
TseI GCWGC 4 cut(s) 56, 155, 191, 361
Tsp45I GTSAC 1 cut(s) 485
Tth111I GACNNNGTC 1 cut(s) 332
VpaK11BI GGWCC 1 cut(s) 396
XspI CTAG 3 cut(s) 165, 237, 384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.