pycom16g10000

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
N/A
Physical Location & Seq
Forward (+)
6786172 .. 6788707
2536 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 666 bp
ATGGGCCTATTTTATCGGCCCAACTTAAAGCCCATCATCGACCCCAAATCCATTCCTACCAGTATCTTTTTAGCTCGATCTCACTCTCGCTTCTTCCTCTTTGGTCTTCGTCTGCACGATCATCAACCTCTCAAGAGGATTTTAAGAGCTATGAGGCTTGACACTAGCGGTCTTGAGTCACACGCGCCGCTGTTCGGCGAGAGCAGTGGGTTTCTTGACGGCGTTTCGGCTCCTCCGTCGTTCGAGGTTCCGACGACGAATGATTTTGATGGATTTCAGAAGGAAGCTATTCAGATGGTGAAGCCGGCTAAGGGGACAACCACACTTGCGTTCATCTTCAAGGATGGAGTCATGGTTGCTGCCGATTCTCGGGCCAGCATGGGCGGATATATCTCATCACAATCTGTGAAGAAGATTATTGAAATCAATCCTTACATGCTTGGTACAATGGCTGGAGGAGCTGCTGACTGCCAGTTTTGGCATAGGAATCTGGGGATTAAGTGTCGGTTGCATGAGTTGGCAAATAAGCGGAGGATTTCAGTTACAGGAGCATCAAAGCTGCTGGCAAACATTCTTTACTCTTATCGTGGTATGGGTCTCTCAGTTGGGACCATCGATCGCAGGCTGGGACGAAACGGGTCCTGGACTGTATTATGTAGACAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000502 GO:0005575 GO:0005622 GO:0005623 GO:0005839 GO:0019774 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1905368 GO:1905369
KEGG Pathways
Metabolic & Signaling
ko03050 map03050
Pfam Domains
Protein Families
Proteasome

Protein Analysis

222

Amino Acids

24.44

Weight (kDa)

10.06

Isoelectric Point (pI)

38.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 104 - 204 9.6e-26 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 658
AccII CGCG 1 cut(s) 185
AciI CCGC 4 cut(s) 168, 188, 384, 529
AfaI GTAC 1 cut(s) 445
AfiI CCNNNNNNNGG 3 cut(s) 194, 311, 369
AgsI TTSAA 2 cut(s) 340, 422
AjnI CCWGG 1 cut(s) 641
AluBI AGCT 5 cut(s) 74, 149, 287, 461, 559
AluI AGCT 5 cut(s) 74, 149, 287, 461, 559
Alw26I GTCTC 1 cut(s) 602
Ama87I CYCGRG 1 cut(s) 369
AoxI GGCC 3 cut(s) 4, 17, 372
ApeKI GCWGC 3 cut(s) 359, 461, 559
Asp700I GAANNNNTTC 1 cut(s) 288
AspLEI GCGC 1 cut(s) 187
AspS9I GGNCC 5 cut(s) 4, 18, 372, 609, 639
AsuHPI GGTGA 1 cut(s) 310
AvaI CYCGRG 1 cut(s) 369
AvaII GGWCC 2 cut(s) 609, 639
BbsI GAAGAC 1 cut(s) 98
BbvI GCAGC 3 cut(s) 346, 448, 546
BccI CCATC 5 cut(s) 41, 263, 289, 338, 620
BceAI ACGGC 1 cut(s) 235
BciT130I CCWGG 1 cut(s) 643
BcoDI GTCTC 1 cut(s) 602
BfaI CTAG 1 cut(s) 165
BisI GCNGC 4 cut(s) 188, 360, 462, 560
BlsI GCNGC 4 cut(s) 189, 361, 463, 561
Bme1390I CCNGG 1 cut(s) 643
Bme18I GGWCC 2 cut(s) 609, 639
BmeT110I CYCGRG 1 cut(s) 369
BmgT120I GGNCC 5 cut(s) 4, 18, 372, 609, 639
BmiI GGNNCC 4 cut(s) 231, 249, 610, 640
BmrFI CCNGG 1 cut(s) 643
BmsI GCATC 1 cut(s) 560
BpiI GAAGAC 1 cut(s) 98
BpmI CTGGAG 1 cut(s) 474
Bpu10I CCTNAGC 1 cut(s) 309
BpuEI CTTGAG 2 cut(s) 116, 194
Bsa29I ATCGAT 1 cut(s) 615
BsaI GGTCTC 1 cut(s) 602
BsaXI ACNNNNNCTCC 2 cut(s) 339, 369
Bsc4I CCNNNNNNNGG 3 cut(s) 194, 311, 369
Bse118I RCCGGY 1 cut(s) 304
Bse1I ACTGG 2 cut(s) 60, 472
BseBI CCWGG 1 cut(s) 643
BseCI ATCGAT 1 cut(s) 615
BseGI GGATG 1 cut(s) 349
BseLI CCNNNNNNNGG 3 cut(s) 194, 311, 369
BseMII CTCAG 1 cut(s) 615
BseNI ACTGG 2 cut(s) 60, 472
BseRI GAGGAG 2 cut(s) 222, 471
BseXI GCAGC 3 cut(s) 346, 448, 546
BseYI CCCAGC 1 cut(s) 625
BsgI GTGCAG 1 cut(s) 98
Bsh1236I CGCG 1 cut(s) 185
Bsh1285I CGRYCG 1 cut(s) 619
BshFI GGCC 3 cut(s) 6, 19, 374
BshVI ATCGAT 1 cut(s) 615
BsiEI CGRYCG 1 cut(s) 619
BsiHKCI CYCGRG 1 cut(s) 369
BsiSI CCGG 1 cut(s) 305
BslFI GGGAC 3 cut(s) 328, 622, 642
BslI CCNNNNNNNGG 3 cut(s) 194, 311, 369
BsmAI GTCTC 1 cut(s) 602
BsmFI GGGAC 3 cut(s) 328, 622, 642
BsnI GGCC 3 cut(s) 6, 19, 374
Bso31I GGTCTC 1 cut(s) 602
BsoBI CYCGRG 1 cut(s) 369
Bsp143I GATC 3 cut(s) 77, 118, 616
BspACI CCGC 4 cut(s) 168, 188, 384, 529
BspANI GGCC 3 cut(s) 6, 19, 374
BspCNI CTCAG 1 cut(s) 614
BspDI ATCGAT 1 cut(s) 615
BspFNI CGCG 1 cut(s) 185
BspLI GGNNCC 4 cut(s) 231, 249, 610, 640
BspTNI GGTCTC 1 cut(s) 602
BsrFI RCCGGY 1 cut(s) 304
BsrI ACTGG 2 cut(s) 60, 472
BssAI RCCGGY 1 cut(s) 304
BssMI GATC 3 cut(s) 77, 118, 616
Bst2UI CCWGG 1 cut(s) 643
Bst4CI ACNGT 2 cut(s) 649, 663
BstC8I GCNNGC 4 cut(s) 306, 376, 564, 623
BstDEI CTNAG 2 cut(s) 309, 601
BstF5I GGATG 1 cut(s) 349
BstFNI CGCG 1 cut(s) 185
BstHHI GCGC 1 cut(s) 187
BstKTI GATC 3 cut(s) 80, 121, 619
BstMAI GTCTC 1 cut(s) 602
BstMBI GATC 3 cut(s) 77, 118, 616
BstMCI CGRYCG 1 cut(s) 619
BstMWI GCNNNNNNNGC 1 cut(s) 458
BstNI CCWGG 1 cut(s) 643
BstNSI RCATGY 1 cut(s) 439
BstSCI CCNGG 1 cut(s) 641
BstUI CGCG 1 cut(s) 185
BstV1I GCAGC 3 cut(s) 346, 448, 546
BstV2I GAAGAC 1 cut(s) 98
Bsu15I ATCGAT 1 cut(s) 615
BsuRI GGCC 3 cut(s) 6, 19, 374
BsuTUI ATCGAT 1 cut(s) 615
BtsCI GGATG 1 cut(s) 349
BtsI GCAGTG 1 cut(s) 211
BtsIMutI CAGTG 1 cut(s) 211
Cac8I GCNNGC 4 cut(s) 306, 376, 564, 623
CfoI GCGC 1 cut(s) 187
Cfr10I RCCGGY 1 cut(s) 304
Cfr13I GGNCC 5 cut(s) 4, 18, 372, 609, 639
ClaI ATCGAT 1 cut(s) 615
Csp6I GTAC 1 cut(s) 444
CviAII CATG 4 cut(s) 352, 379, 436, 512
CviQI GTAC 1 cut(s) 444
DdeI CTNAG 2 cut(s) 309, 601
DpnI GATC 3 cut(s) 79, 120, 618
DpnII GATC 3 cut(s) 77, 118, 616
EciI GGCGGA 1 cut(s) 399
Eco31I GGTCTC 1 cut(s) 602
Eco47I GGWCC 2 cut(s) 609, 639
Eco88I CYCGRG 1 cut(s) 369
EcoO109I RGGNCCY 1 cut(s) 639
EcoRII CCWGG 1 cut(s) 641
FaeI CATG 4 cut(s) 355, 382, 439, 515
FaiI YATR 9 cut(s) 152, 353, 380, 390, 437, 483, 513, 593, 655
FaqI GGGAC 3 cut(s) 328, 622, 642
FatI CATG 4 cut(s) 351, 378, 435, 511
FblI GTMKAC 1 cut(s) 658
Fnu4HI GCNGC 4 cut(s) 188, 360, 462, 560
FokI GGATG 1 cut(s) 356
Fsp4HI GCNGC 4 cut(s) 188, 360, 462, 560
FspBI CTAG 1 cut(s) 165
GlaI GCGC 1 cut(s) 186
GluI GCNGC 4 cut(s) 188, 360, 462, 560
GsaI CCCAGC 1 cut(s) 629
GsuI CTGGAG 1 cut(s) 474
HaeIII GGCC 3 cut(s) 6, 19, 374
HapII CCGG 1 cut(s) 305
HhaI GCGC 1 cut(s) 187
Hin1II CATG 4 cut(s) 355, 382, 439, 515
Hin6I GCGC 1 cut(s) 185
HinP1I GCGC 1 cut(s) 185
HinfI GANTC 4 cut(s) 176, 348, 365, 487
HpaII CCGG 1 cut(s) 305
HphI GGTGA 1 cut(s) 310
Hpy166II GTNNAC 1 cut(s) 659
Hpy188I TCNGA 3 cut(s) 252, 279, 294
Hpy188III TCNNGA 3 cut(s) 133, 173, 215
Hpy8I GTNNAC 1 cut(s) 659
Hpy99I CGWCG 3 cut(s) 241, 256, 259
HpyAV CCTTC 1 cut(s) 274
HpyCH4III ACNGT 2 cut(s) 649, 663
HpyCH4V TGCA 2 cut(s) 115, 511
HpyF10VI GCNNNNNNNGC 1 cut(s) 458
HpyF3I CTNAG 2 cut(s) 309, 601
Hsp92II CATG 4 cut(s) 355, 382, 439, 515
HspAI GCGC 1 cut(s) 185
KroI GCCGGC 1 cut(s) 304
KroNI GCCGGC 1 cut(s) 306
Kzo9I GATC 3 cut(s) 77, 118, 616
LmnI GCTCC 3 cut(s) 235, 458, 548
Lsp1109I GCAGC 3 cut(s) 346, 448, 546
LweI GCATC 1 cut(s) 560
MaeI CTAG 1 cut(s) 165
MaeIII GTNAC 2 cut(s) 177, 541
MalI GATC 3 cut(s) 79, 120, 618
MboI GATC 3 cut(s) 77, 118, 616
MboII GAAGA 5 cut(s) 85, 98, 328, 421, 424
MlyI GAGTC 2 cut(s) 185, 357
MmeI TCCRAC 1 cut(s) 275
MnlI CCTC 8 cut(s) 107, 129, 138, 147, 238, 243, 449, 525
MroNI GCCGGC 1 cut(s) 304
MroXI GAANNNNTTC 1 cut(s) 288
MseI TTAA 3 cut(s) 26, 143, 498
MspA1I CMGCKG 1 cut(s) 190
MspI CCGG 1 cut(s) 305
MspR9I CCNGG 1 cut(s) 643
MvaI CCWGG 1 cut(s) 643
MvnI CGCG 1 cut(s) 185
MwoI GCNNNNNNNGC 1 cut(s) 458
NaeI GCCGGC 1 cut(s) 306
NdeII GATC 3 cut(s) 77, 118, 616
NgoMIV GCCGGC 1 cut(s) 304
NlaIII CATG 4 cut(s) 355, 382, 439, 515
NlaIV GGNNCC 4 cut(s) 231, 249, 610, 640
NmuCI GTSAC 1 cut(s) 177
NspI RCATGY 1 cut(s) 439
PcsI WCGNNNNNNNCGW 1 cut(s) 233
PdiI GCCGGC 1 cut(s) 306
PdmI GAANNNNTTC 1 cut(s) 288
PfeI GAWTC 2 cut(s) 365, 487
PfoI TCCNGGA 1 cut(s) 641
PkrI GCNGC 4 cut(s) 189, 361, 463, 561
Ple19I CGATCG 1 cut(s) 619
PleI GAGTC 2 cut(s) 184, 356
PpsI GAGTC 2 cut(s) 184, 356
PpuMI RGGWCCY 1 cut(s) 639
Psp5II RGGWCCY 1 cut(s) 639
Psp6I CCWGG 1 cut(s) 641
PspFI CCCAGC 1 cut(s) 625
PspGI CCWGG 1 cut(s) 641
PspN4I GGNNCC 4 cut(s) 231, 249, 610, 640
PspPI GGNCC 5 cut(s) 4, 18, 372, 609, 639
PspPPI RGGWCCY 1 cut(s) 639
PvuI CGATCG 1 cut(s) 619
RsaI GTAC 1 cut(s) 445
RsaNI GTAC 1 cut(s) 444
SaqAI TTAA 3 cut(s) 26, 143, 498
SatI GCNGC 4 cut(s) 188, 360, 462, 560
Sau3AI GATC 3 cut(s) 77, 118, 616
Sau96I GGNCC 5 cut(s) 4, 18, 372, 609, 639
SchI GAGTC 2 cut(s) 185, 357
ScrFI CCNGG 1 cut(s) 643
SetI ASST 7 cut(s) 76, 130, 151, 249, 289, 463, 561
SfaNI GCATC 1 cut(s) 560
SinI GGWCC 2 cut(s) 609, 639
SmlI CTYRAG 2 cut(s) 131, 173
SmoI CTYRAG 2 cut(s) 131, 173
SsiI CCGC 4 cut(s) 168, 188, 384, 529
SspMI CTAG 1 cut(s) 165
StyD4I CCNGG 1 cut(s) 641
TaaI ACNGT 2 cut(s) 649, 663
TaqI TCGA 4 cut(s) 39, 76, 243, 615
TauI GCSGC 1 cut(s) 190
TfiI GAWTC 2 cut(s) 365, 487
Tru1I TTAA 3 cut(s) 26, 143, 498
Tru9I TTAA 3 cut(s) 26, 143, 498
TscAI CASTG 1 cut(s) 211
TseFI GTSAC 1 cut(s) 177
TseI GCWGC 3 cut(s) 359, 461, 559
Tsp45I GTSAC 1 cut(s) 177
TspDTI ATGAA 1 cut(s) 322
TspGWI ACGGA 1 cut(s) 225
TspRI CASTG 1 cut(s) 211
VpaK11BI GGWCC 2 cut(s) 609, 639
XceI RCATGY 1 cut(s) 439
XmiI GTMKAC 1 cut(s) 658
XmnI GAANNNNTTC 1 cut(s) 288
XspI CTAG 1 cut(s) 165
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.