pycom16g10340

Histidine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
N/A
Physical Location & Seq
Forward (+)
7055168 .. 7056010
843 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 423 bp
ATGCGGTGCAGGTTCAAACATAAGCCACCTTGGCCGTGGTTAGCCATTACAACTTCTATTGGGATCCTCGTGATTGCATTGCTTGTTGGCTACATTTTCCATGCAACTGTAAATAGGATTGCCAATGTGGAGGATGATTTCCATAAGATGATGGAGCTCAAAAAACAGGCTGAGGCAGCTGATGTTGCCAAATCTCAGTTTCTTGCAACTGTTTCACACGAGATCAGAACCCCAATGAATGGTGTCCTAGGAATGTTACACATGCTAATGGACACAAACCTGGATGTTACTCAACTAGATTACGTCCGAACTGCCCAGGGAAGTGGAAAAGCTCTAGTCTCACTTATAAATGAGGTATTGGACCAGGCGAAGATTGAATCTGGTAAGCTGGAGCTTGAGGCAGTGCGTTCACACAGAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004673 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009266 GO:0009267 GO:0009409 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009735 GO:0009736 GO:0009737 GO:0009743 GO:0009744 GO:0009755 GO:0009884 GO:0009888 GO:0009894 GO:0009909 GO:0009987 GO:0009991 GO:0010029 GO:0010033 GO:0010035 GO:0010087 GO:0010150 GO:0010271 GO:0010959 GO:0012505 GO:0016020 GO:0016036 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016775 GO:0018106 GO:0018193 GO:0018202 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0019901 GO:0023052 GO:0031323 GO:0031329 GO:0031667 GO:0031668 GO:0031669 GO:0031984 GO:0032501 GO:0032502 GO:0032870 GO:0032879 GO:0033554 GO:0033993 GO:0034285 GO:0034756 GO:0034757 GO:0036211 GO:0038023 GO:0040007 GO:0042175 GO:0042221 GO:0042594 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043269 GO:0043271 GO:0043412 GO:0043424 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0048366 GO:0048367 GO:0048509 GO:0048519 GO:0048580 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051049 GO:0051051 GO:0051171 GO:0051193 GO:0051239 GO:0051704 GO:0051707 GO:0051716 GO:0060089 GO:0065007 GO:0070417 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071322 GO:0071324 GO:0071329 GO:0071368 GO:0071396 GO:0071495 GO:0071496 GO:0071704 GO:0071944 GO:0080117 GO:0080190 GO:0090056 GO:0090693 GO:0097305 GO:0097306 GO:0098542 GO:0098827 GO:0099402 GO:0140096 GO:1900140 GO:1901401 GO:1901404 GO:1901564 GO:1901700 GO:1901701 GO:2000026 GO:2000241
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
CHASE HATPase_c HisKA Response_reg

Protein Analysis

141

Amino Acids

15.71

Weight (kDa)

7.02

Isoelectric Point (pI)

18.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HisKA PF00512 63 - 128 1.6e-19 His Kinase A (phospho-acceptor) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 347
AccB7I CCANNNNNTGG 1 cut(s) 239
AciI CCGC 1 cut(s) 4
AclWI GGATC 2 cut(s) 58, 71
AcoI YGGCCR 1 cut(s) 32
AfiI CCNNNNNNNGG 1 cut(s) 239
AgsI TTSAA 2 cut(s) 16, 377
AjnI CCWGG 3 cut(s) 279, 315, 363
AluBI AGCT 5 cut(s) 157, 179, 332, 388, 394
AluI AGCT 5 cut(s) 157, 179, 332, 388, 394
Alw21I GWGCWC 1 cut(s) 159
Alw26I GTCTC 1 cut(s) 343
AlwI GGATC 2 cut(s) 58, 71
AoxI GGCC 1 cut(s) 32
ApeKI GCWGC 1 cut(s) 176
AspA2I CCTAGG 1 cut(s) 247
AspS9I GGNCC 1 cut(s) 361
AvaII GGWCC 1 cut(s) 361
AvrII CCTAGG 1 cut(s) 247
BamHI GGATCC 1 cut(s) 63
BanII GRGCYC 1 cut(s) 159
BauI CACGAG 2 cut(s) 68, 218
Bbv12I GWGCWC 1 cut(s) 159
BbvCI CCTCAGC 1 cut(s) 171
BbvI GCAGC 1 cut(s) 188
BccI CCATC 1 cut(s) 145
BceAI ACGGC 1 cut(s) 19
BciT130I CCWGG 3 cut(s) 281, 317, 365
BcoDI GTCTC 1 cut(s) 343
BfaI CTAG 3 cut(s) 248, 296, 335
BglI GCCNNNNNGGC 1 cut(s) 31
BisI GCNGC 1 cut(s) 177
BlnI CCTAGG 1 cut(s) 247
BlsI GCNGC 1 cut(s) 178
Bme1390I CCNGG 3 cut(s) 281, 317, 365
Bme18I GGWCC 1 cut(s) 361
BmgT120I GGNCC 1 cut(s) 361
BmiI GGNNCC 1 cut(s) 65
BmrFI CCNGG 3 cut(s) 281, 317, 365
BpmI CTGGAG 1 cut(s) 410
Bpu10I CCTNAGC 1 cut(s) 171
BpuEI CTTGAG 1 cut(s) 416
BsaJI CCNNGG 5 cut(s) 29, 35, 247, 315, 316
Bsc4I CCNNNNNNNGG 1 cut(s) 239
Bse3DI GCAATG 1 cut(s) 77
BseBI CCWGG 3 cut(s) 281, 317, 365
BseDI CCNNGG 5 cut(s) 29, 35, 247, 315, 316
BseGI GGATG 2 cut(s) 139, 289
BseLI CCNNNNNNNGG 1 cut(s) 239
BseMI GCAATG 1 cut(s) 77
BseMII CTCAG 2 cut(s) 162, 209
BseXI GCAGC 1 cut(s) 188
BsgI GTGCAG 1 cut(s) 28
BshFI GGCC 1 cut(s) 34
BsiHKAI GWGCWC 1 cut(s) 159
BslI CCNNNNNNNGG 1 cut(s) 239
BsmAI GTCTC 1 cut(s) 343
BsnI GGCC 1 cut(s) 34
Bsp1286I GDGCHC 1 cut(s) 159
Bsp143I GATC 2 cut(s) 63, 222
BspACI CCGC 1 cut(s) 4
BspANI GGCC 1 cut(s) 34
BspCNI CTCAG 2 cut(s) 163, 208
BspLI GGNNCC 1 cut(s) 65
BspPI GGATC 2 cut(s) 58, 71
BsrDI GCAATG 1 cut(s) 77
BssECI CCNNGG 5 cut(s) 29, 35, 247, 315, 316
BssMI GATC 2 cut(s) 63, 222
BssSI CACGAG 2 cut(s) 68, 218
BssT1I CCWWGG 2 cut(s) 29, 247
Bst2BI CACGAG 2 cut(s) 68, 218
Bst2UI CCWGG 3 cut(s) 281, 317, 365
Bst4CI ACNGT 2 cut(s) 109, 211
BstDEI CTNAG 2 cut(s) 171, 195
BstDSI CCRYGG 1 cut(s) 35
BstF5I GGATG 2 cut(s) 139, 289
BstKTI GATC 2 cut(s) 66, 225
BstMAI GTCTC 1 cut(s) 343
BstMBI GATC 2 cut(s) 63, 222
BstMWI GCNNNNNNNGC 3 cut(s) 31, 176, 185
BstNI CCWGG 3 cut(s) 281, 317, 365
BstNSI RCATGY 1 cut(s) 265
BstSCI CCNGG 3 cut(s) 279, 315, 363
BstV1I GCAGC 1 cut(s) 188
BstX2I RGATCY 1 cut(s) 63
BstXI CCANNNNNNTGG 1 cut(s) 323
BstYI RGATCY 1 cut(s) 63
BsuRI GGCC 1 cut(s) 34
BtgI CCRYGG 1 cut(s) 35
BtsCI GGATG 2 cut(s) 139, 289
BtsI GCAGTG 1 cut(s) 408
BtsIMutI CAGTG 1 cut(s) 408
Cfr13I GGNCC 1 cut(s) 361
CviAII CATG 2 cut(s) 101, 262
DdeI CTNAG 2 cut(s) 171, 195
DpnI GATC 2 cut(s) 65, 224
DpnII GATC 2 cut(s) 63, 222
EaeI YGGCCR 1 cut(s) 32
Ecl136II GAGCTC 1 cut(s) 157
Eco130I CCWWGG 2 cut(s) 29, 247
Eco24I GRGCYC 1 cut(s) 159
Eco47I GGWCC 1 cut(s) 361
Eco53kI GAGCTC 1 cut(s) 157
EcoICRI GAGCTC 1 cut(s) 157
EcoRII CCWGG 3 cut(s) 279, 315, 363
EcoT14I CCWWGG 2 cut(s) 29, 247
EcoT38I GRGCYC 1 cut(s) 159
ErhI CCWWGG 2 cut(s) 29, 247
FaeI CATG 2 cut(s) 104, 265
FaiI YATR 5 cut(s) 21, 102, 144, 263, 347
FatI CATG 2 cut(s) 100, 261
Fnu4HI GCNGC 1 cut(s) 177
FokI GGATG 2 cut(s) 146, 296
FriOI GRGCYC 1 cut(s) 159
Fsp4HI GCNGC 1 cut(s) 177
FspBI CTAG 3 cut(s) 248, 296, 335
GluI GCNGC 1 cut(s) 177
GsuI CTGGAG 1 cut(s) 410
HaeIII GGCC 1 cut(s) 34
Hin1II CATG 2 cut(s) 104, 265
HinfI GANTC 1 cut(s) 377
Hpy166II GTNNAC 1 cut(s) 410
Hpy188I TCNGA 2 cut(s) 227, 308
Hpy188III TCNNGA 1 cut(s) 70
Hpy8I GTNNAC 1 cut(s) 410
HpyCH4III ACNGT 2 cut(s) 109, 211
HpyCH4IV ACGT 1 cut(s) 303
HpyCH4V TGCA 4 cut(s) 9, 77, 104, 206
HpyF10VI GCNNNNNNNGC 3 cut(s) 31, 176, 185
HpyF3I CTNAG 2 cut(s) 171, 195
HpySE526I ACGT 1 cut(s) 303
Hsp92II CATG 2 cut(s) 104, 265
Kzo9I GATC 2 cut(s) 63, 222
LmnI GCTCC 2 cut(s) 154, 391
LpnPI CCDG 9 cut(s) 152, 266, 293, 302, 329, 350, 366, 374, 377
Lsp1109I GCAGC 1 cut(s) 188
MaeI CTAG 3 cut(s) 248, 296, 335
MaeII ACGT 1 cut(s) 303
MaeIII GTNAC 2 cut(s) 255, 286
MalI GATC 2 cut(s) 65, 224
MboI GATC 2 cut(s) 63, 222
MboII GAAGA 1 cut(s) 382
MflI RGATCY 1 cut(s) 63
MhlI GDGCHC 1 cut(s) 159
MnlI CCTC 5 cut(s) 77, 124, 166, 346, 391
MslI CAYNNNNRTG 1 cut(s) 266
MspA1I CMGCKG 1 cut(s) 179
MspR9I CCNGG 3 cut(s) 281, 317, 365
MvaI CCWGG 3 cut(s) 281, 317, 365
MwoI GCNNNNNNNGC 3 cut(s) 31, 176, 185
NdeII GATC 2 cut(s) 63, 222
NlaIII CATG 2 cut(s) 104, 265
NlaIV GGNNCC 1 cut(s) 65
NspI RCATGY 1 cut(s) 265
PasI CCCWGGG 1 cut(s) 316
PfeI GAWTC 1 cut(s) 377
PflMI CCANNNNNTGG 1 cut(s) 239
PkrI GCNGC 1 cut(s) 178
PsiI TTATAA 1 cut(s) 347
Psp124BI GAGCTC 1 cut(s) 159
Psp6I CCWGG 3 cut(s) 279, 315, 363
PspGI CCWGG 3 cut(s) 279, 315, 363
PspN4I GGNNCC 1 cut(s) 65
PspPI GGNCC 1 cut(s) 361
PsuI RGATCY 1 cut(s) 63
PvuII CAGCTG 1 cut(s) 179
RseI CAYNNNNRTG 1 cut(s) 266
SacI GAGCTC 1 cut(s) 159
SatI GCNGC 1 cut(s) 177
Sau3AI GATC 2 cut(s) 63, 222
Sau96I GGNCC 1 cut(s) 361
ScrFI CCNGG 3 cut(s) 281, 317, 365
SduI GDGCHC 1 cut(s) 159
SinI GGWCC 1 cut(s) 361
SmiMI CAYNNNNRTG 1 cut(s) 266
SmlI CTYRAG 1 cut(s) 395
SmoI CTYRAG 1 cut(s) 395
SsiI CCGC 1 cut(s) 4
SspMI CTAG 3 cut(s) 248, 296, 335
SstI GAGCTC 1 cut(s) 159
StyD4I CCNGG 3 cut(s) 279, 315, 363
StyI CCWWGG 2 cut(s) 29, 247
TaaI ACNGT 2 cut(s) 109, 211
TaiI ACGT 1 cut(s) 306
TfiI GAWTC 1 cut(s) 377
TscAI CASTG 1 cut(s) 408
TseI GCWGC 1 cut(s) 176
TspDTI ATGAA 1 cut(s) 251
TspRI CASTG 1 cut(s) 408
Van91I CCANNNNNTGG 1 cut(s) 239
VpaK11BI GGWCC 1 cut(s) 361
XceI RCATGY 1 cut(s) 265
XcmI CCANNNNNNNNNTGG 1 cut(s) 33
XmaJI CCTAGG 1 cut(s) 247
XspI CTAG 3 cut(s) 248, 296, 335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.