Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr16

Orthogroup ID

N/A

Physical Location & Seq

Genomic Coordinates Reverse (-)

8655540 .. 8658060

Gene Structure 2521 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1068 bp

ATGTCTAAAGAACACAAGTGGCGAGAAGTGGCACGTGGCAGGACTATTTCCACGCGTGTGACTTTACAATCTTATCCAACTGTACACACTCATCAGCCGCACCTGCCAACTGCGCACTTCCCGTTCGCAACAAAACATCATTTGCCACTCCACTCCACCGCACCATACCAGACCCGACCCGACCCGATTGTTCGTATCCGGCGACGCCAGCGTGATCCGAGAAAATCCGACCCAATGGCCCGGAAGAAGATCAGAGAATACGACTCCAAGAGGCTGCTCAAGCAGCACCTGAAACGACTCACCGGAATCGACCTCCAGATCAACTCAGCTCAGGTGACCGAGTCAACGGACTTCACCGAGTTGGCGAACGAGCTGCCATGGCTGTCGTCGACGCGGCTGGTGGTGAAGCCCGACATGCTTTTCGGAAAGCGCGGAAAGAGCGGCCTCGTCGCCCTGAACTTGGACATGGCGCAGGTCGCCGAGTTCGTCAAGCAGCGACTCGGTAAGGAGGTTAAGACTATATTTCTTCCGACCGAGAAGCCTATGACGAATGACGCTTGTGCTCCATTGATTGCCACTCTTCCATTGGAGGTCAGAGACAAAATTGGGGATTTCATAAAGGGCGTATTTTCTGTATTCCAGGATTTGGATTTTAGTTTCCTGGAAATGAACCCATTTACCTTGGTTGACGGTGAACCTTATCCATTAGATATGAGGGGGGAGCTGGATGACACTGCTGCCTTCAAGAACTTCAATACATGGGGCAATGTGGAGTTTCCACTGCCTTTCGGCAGAGTCTTAAGTCCTACAGAAACGTTTATTCATTCTCTGGATGAAAAGACAAGCGCATCCTTAAAATTCACTGTGTTGAACCCAAAAGGACGCATCTGGACCATGGTAGCTGGAGGGGGTGCAAGTGTTATTTATGCTGATACTGTTGGAGATTTGGGTTATGCTTCAGAACTTGGAAATTATGCAGAGTATAGTGGAGCTCCAAATGAGGAAGAGGTGTTGCAATATGCAAGAGTTGTGGTCGATTGTGCAACAGCTGATCTCAGATGGTCGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003878 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006084 GO:0006085 GO:0006139 GO:0006163 GO:0006164 GO:0006629 GO:0006631 GO:0006633 GO:0006637 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008610 GO:0009058 GO:0009108 GO:0009117 GO:0009150 GO:0009152 GO:0009165 GO:0009259 GO:0009260 GO:0009346 GO:0009987 GO:0016053 GO:0016740 GO:0016746 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0032991 GO:0033865 GO:0033866 GO:0033875 GO:0034030 GO:0034032 GO:0034033 GO:0034641 GO:0034654 GO:0035383 GO:0035384 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044271 GO:0044272 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046390 GO:0046394 GO:0046483 GO:0046912 GO:0051186 GO:0051188 GO:0055086 GO:0071616 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0090407 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00020 ko00720 ko01100 ko01110 ko01120 ko01130 map00020 map00720 map01100 map01110 map01120 map01130

Pfam Domains

Protein Families

ATP-grasp_2 Citrate_bind

Protein Analysis

356

Amino Acids

40.1

Weight (kDa)

8.67

Isoelectric Point (pI)

34.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Citrate_synth_N	PF24948	80 - 172	2.5e-26	ATP-citrate synthase ATP-grasp domain
ATP-grasp_2	PF08442	84 - 174	1.8e-06	ATP-grasp domain
Citrate_synth_N	PF24948	173 - 254	5e-17	ATP-citrate synthase ATP-grasp domain
Citrate_bind	PF16114	265 - 350	1.3e-40	ATP citrate lyase citrate-binding

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	111
Acc16I	TGCGCA	1 cut(s)	114
Acc36I	ACCTGC	2 cut(s)	111, 463
AccB7I	CCANNNNNTGG	1 cut(s)	646
AccBSI	CCGCTC	1 cut(s)	441
AccI	GTMKAC	1 cut(s)	389
AccII	CGCG	3 cut(s)	55, 394, 432
AciI	CCGC	5 cut(s)	98, 159, 394, 432, 441
AclI	AACGTT	1 cut(s)	815
AclWI	GGATC	1 cut(s)	209
AcsI	RAATTY	1 cut(s)	857
AcuI	CTGAAG	1 cut(s)	942
AcvI	CACGTG	1 cut(s)	35
AcyI	GRCGYC	1 cut(s)	205
AfaI	GTAC	1 cut(s)	84
AfiI	CCNNNNNNNGG	2 cut(s)	460, 646
AflII	CTTAAG	1 cut(s)	799
AflIII	ACRYGT	1 cut(s)	53
AgsI	TTSAA	3 cut(s)	745, 754, 871
AjnI	CCWGG	2 cut(s)	639, 660
AleI	CACNNNNGTG	1 cut(s)	56
AluBI	AGCT	6 cut(s)	329, 373, 724, 902, 992, 1049
AluI	AGCT	6 cut(s)	329, 373, 724, 902, 992, 1049
Alw21I	GWGCWC	2 cut(s)	565, 994
Alw26I	GTCTC	1 cut(s)	591
AlwI	GGATC	1 cut(s)	209
AlwNI	CAGNNNCTG	1 cut(s)	289
AoxI	GGCC	2 cut(s)	237, 442
ApeKI	GCWGC	5 cut(s)	274, 283, 373, 493, 737
ApoI	RAATTY	1 cut(s)	857
ArsI	GACNNNNNNTTYG	2 cut(s)	404, 436
AspLEI	GCGC	4 cut(s)	115, 432, 472, 848
AspS9I	GGNCC	2 cut(s)	238, 891
AsuC2I	CCSGG	1 cut(s)	241
AsuHPI	GGTGA	5 cut(s)	292, 346, 346, 415, 704
AvaII	GGWCC	1 cut(s)	891
BanII	GRGCYC	1 cut(s)	994
BbrPI	CACGTG	1 cut(s)	35
Bbv12I	GWGCWC	2 cut(s)	565, 994
BbvI	GCAGC	5 cut(s)	261, 295, 360, 505, 724
BccI	CCATC	1 cut(s)	1053
BcgI	CGANNNNNNTGC	2 cut(s)	355, 389
BciT130I	CCWGG	2 cut(s)	641, 662
BciVI	GTATCC	1 cut(s)	206
BcnI	CCSGG	1 cut(s)	241
BcoDI	GTCTC	1 cut(s)	591
BfmI	CTRYAG	1 cut(s)	807
BfrI	CTTAAG	1 cut(s)	799
BfuAI	ACCTGC	2 cut(s)	111, 463
BfuI	GTATCC	1 cut(s)	206
BisI	GCNGC	8 cut(s)	98, 275, 284, 374, 395, 442, 494, 738
BlsI	GCNGC	8 cut(s)	99, 276, 285, 375, 396, 443, 495, 739
Bme1390I	CCNGG	3 cut(s)	241, 641, 662
Bme18I	GGWCC	1 cut(s)	891
BmgT120I	GGNCC	2 cut(s)	238, 891
BmrFI	CCNGG	3 cut(s)	241, 641, 662
BmsI	GCATC	2 cut(s)	857, 894
BpmI	CTGGAG	2 cut(s)	299, 924
Bpu10I	CCTNAGC	1 cut(s)	330
BpuEI	CTTGAG	1 cut(s)	263
BpuMI	CCSGG	1 cut(s)	241
BsaAI	YACGTR	1 cut(s)	35
BsaHI	GRCGYC	1 cut(s)	205
BsaJI	CCNNGG	3 cut(s)	377, 681, 894
BsaWI	WCCGGW	1 cut(s)	302
Bsc4I	CCNNNNNNNGG	2 cut(s)	460, 646
Bse3DI	GCAATG	1 cut(s)	772
BseBI	CCWGG	2 cut(s)	641, 662
BseDI	CCNNGG	3 cut(s)	377, 681, 894
BseGI	GGATG	3 cut(s)	733, 838, 848
BseLI	CCNNNNNNNGG	2 cut(s)	460, 646
BseMI	GCAATG	1 cut(s)	772
BseMII	CTCAG	2 cut(s)	339, 344
BseXI	GCAGC	5 cut(s)	261, 295, 360, 505, 724
Bsh1236I	CGCG	3 cut(s)	55, 394, 432
Bsh1285I	CGRYCG	1 cut(s)	534
BshFI	GGCC	2 cut(s)	239, 444
BsiEI	CGRYCG	1 cut(s)	534
BsiHKAI	GWGCWC	2 cut(s)	565, 994
BsiSI	CCGG	3 cut(s)	199, 241, 303
BslI	CCNNNNNNNGG	2 cut(s)	460, 646
BsmAI	GTCTC	1 cut(s)	591
BsnI	GGCC	2 cut(s)	239, 444
Bsp1286I	GDGCHC	2 cut(s)	565, 994
Bsp1407I	TGTACA	1 cut(s)	82
Bsp143I	GATC	4 cut(s)	214, 249, 318, 1051
Bsp19I	CCATGG	2 cut(s)	377, 894
BspACI	CCGC	5 cut(s)	98, 159, 394, 432, 441
BspANI	GGCC	2 cut(s)	239, 444
BspCNI	CTCAG	3 cut(s)	338, 343, 1068
BspFNI	CGCG	3 cut(s)	55, 394, 432
BspMI	ACCTGC	2 cut(s)	111, 463
BspPI	GGATC	1 cut(s)	209
BspTI	CTTAAG	1 cut(s)	799
BsrBI	CCGCTC	1 cut(s)	441
BsrDI	GCAATG	1 cut(s)	772
BsrGI	TGTACA	1 cut(s)	82
BssECI	CCNNGG	3 cut(s)	377, 681, 894
BssMI	GATC	4 cut(s)	214, 249, 318, 1051
BssNI	GRCGYC	1 cut(s)	205
BssT1I	CCWWGG	3 cut(s)	377, 681, 894
Bst2UI	CCWGG	2 cut(s)	641, 662
Bst4CI	ACNGT	4 cut(s)	82, 692, 865, 937
Bst6I	CTCTTC	2 cut(s)	585, 999
BstACI	GRCGYC	1 cut(s)	205
BstAFI	CTTAAG	1 cut(s)	799
BstAUI	TGTACA	1 cut(s)	82
BstBAI	YACGTR	1 cut(s)	35
BstC8I	GCNNGC	1 cut(s)	209
BstDEI	CTNAG	3 cut(s)	325, 330, 1055
BstDSI	CCRYGG	2 cut(s)	377, 894
BstEII	GGTNACC	1 cut(s)	334
BstF5I	GGATG	3 cut(s)	733, 838, 848
BstFNI	CGCG	3 cut(s)	55, 394, 432
BstHHI	GCGC	4 cut(s)	115, 432, 472, 848
BstKTI	GATC	4 cut(s)	217, 252, 321, 1054
BstMAI	GTCTC	1 cut(s)	591
BstMBI	GATC	4 cut(s)	214, 249, 318, 1051
BstMCI	CGRYCG	1 cut(s)	534
BstMWI	GCNNNNNNNGC	9 cut(s)	103, 112, 208, 280, 283, 379, 415, 438, 476
BstNI	CCWGG	2 cut(s)	641, 662
BstNSI	RCATGY	1 cut(s)	418
BstPI	GGTNACC	1 cut(s)	334
BstSCI	CCNGG	3 cut(s)	239, 639, 660
BstSFI	CTRYAG	1 cut(s)	807
BstUI	CGCG	3 cut(s)	55, 394, 432
BstV1I	GCAGC	5 cut(s)	261, 295, 360, 505, 724
BsuI	GTATCC	1 cut(s)	206
BsuRI	GGCC	2 cut(s)	239, 444
BtgI	CCRYGG	2 cut(s)	377, 894
BtsCI	GGATG	3 cut(s)	733, 838, 848
BtsI	GCAGTG	2 cut(s)	732, 779
BtsIMutI	CAGTG	3 cut(s)	732, 779, 861
BveI	ACCTGC	2 cut(s)	111, 463
Cac8I	GCNNGC	1 cut(s)	209
CaiI	CAGNNNCTG	1 cut(s)	289
CfoI	GCGC	4 cut(s)	115, 432, 472, 848
Cfr13I	GGNCC	2 cut(s)	238, 891
CseI	GACGC	4 cut(s)	213, 400, 563, 891
Csp6I	GTAC	1 cut(s)	83
CspCI	CAANNNNNGTGG	2 cut(s)	1011, 1046
CviAII	CATG	5 cut(s)	378, 415, 466, 759, 895
CviQI	GTAC	1 cut(s)	83
DdeI	CTNAG	3 cut(s)	325, 330, 1055
DpnI	GATC	4 cut(s)	216, 251, 320, 1053
DpnII	GATC	4 cut(s)	214, 249, 318, 1051
Eam1104I	CTCTTC	2 cut(s)	585, 999
EarI	CTCTTC	2 cut(s)	585, 999
Ecl136II	GAGCTC	1 cut(s)	992
Eco130I	CCWWGG	3 cut(s)	377, 681, 894
Eco24I	GRGCYC	1 cut(s)	994
Eco47I	GGWCC	1 cut(s)	891
Eco53kI	GAGCTC	1 cut(s)	992
Eco57I	CTGAAG	1 cut(s)	942
Eco72I	CACGTG	1 cut(s)	35
Eco91I	GGTNACC	1 cut(s)	334
EcoICRI	GAGCTC	1 cut(s)	992
EcoO65I	GGTNACC	1 cut(s)	334
EcoRII	CCWGG	2 cut(s)	639, 660
EcoT14I	CCWWGG	3 cut(s)	377, 681, 894
EcoT38I	GRGCYC	1 cut(s)	994
ErhI	CCWWGG	3 cut(s)	377, 681, 894
FaeI	CATG	5 cut(s)	381, 418, 469, 762, 898
FatI	CATG	5 cut(s)	377, 414, 465, 758, 894
FblI	GTMKAC	1 cut(s)	389
Fnu4HI	GCNGC	8 cut(s)	98, 275, 284, 374, 395, 442, 494, 738
FokI	GGATG	3 cut(s)	740, 835, 845
FriOI	GRGCYC	1 cut(s)	994
Fsp4HI	GCNGC	8 cut(s)	98, 275, 284, 374, 395, 442, 494, 738
FspI	TGCGCA	1 cut(s)	114
GlaI	GCGC	4 cut(s)	114, 431, 471, 847
GluI	GCNGC	8 cut(s)	98, 275, 284, 374, 395, 442, 494, 738
GsuI	CTGGAG	2 cut(s)	299, 924
HaeIII	GGCC	2 cut(s)	239, 444
HapII	CCGG	3 cut(s)	199, 241, 303
HgaI	GACGC	4 cut(s)	213, 400, 563, 891
HhaI	GCGC	4 cut(s)	115, 432, 472, 848
Hin1I	GRCGYC	1 cut(s)	205
Hin1II	CATG	5 cut(s)	381, 418, 469, 762, 898
Hin6I	GCGC	4 cut(s)	113, 430, 470, 846
HinP1I	GCGC	4 cut(s)	113, 430, 470, 846
HincII	GTYRAC	3 cut(s)	345, 390, 688
HindII	GTYRAC	3 cut(s)	345, 390, 688
HinfI	GANTC	6 cut(s)	263, 297, 306, 341, 498, 795
HpaII	CCGG	3 cut(s)	199, 241, 303
HphI	GGTGA	5 cut(s)	292, 346, 346, 415, 704
Hpy166II	GTNNAC	5 cut(s)	85, 345, 390, 688, 695
Hpy188I	TCNGA	8 cut(s)	219, 229, 254, 425, 531, 596, 961, 1058
Hpy188III	TCNNGA	4 cut(s)	316, 745, 830, 889
Hpy8I	GTNNAC	5 cut(s)	85, 345, 390, 688, 695
Hpy99I	CGWCG	4 cut(s)	207, 391, 394, 452
HpyAV	CCTTC	1 cut(s)	751
HpyCH4III	ACNGT	4 cut(s)	82, 692, 865, 937
HpyCH4IV	ACGT	2 cut(s)	34, 815
HpyCH4V	TGCA	5 cut(s)	914, 977, 1015, 1022, 1043
HpyF10VI	GCNNNNNNNGC	9 cut(s)	103, 112, 208, 280, 283, 379, 415, 438, 476
HpyF3I	CTNAG	3 cut(s)	325, 330, 1055
HpySE526I	ACGT	2 cut(s)	34, 815
Hsp92I	GRCGYC	1 cut(s)	205
Hsp92II	CATG	5 cut(s)	381, 418, 469, 762, 898
HspAI	GCGC	4 cut(s)	113, 430, 470, 846
Kzo9I	GATC	4 cut(s)	214, 249, 318, 1051
LmnI	GCTCC	4 cut(s)	568, 721, 989, 997
Lsp1109I	GCAGC	5 cut(s)	261, 295, 360, 505, 724
LweI	GCATC	2 cut(s)	857, 894
MaeII	ACGT	2 cut(s)	34, 815
MaeIII	GTNAC	2 cut(s)	58, 334
MalI	GATC	4 cut(s)	216, 251, 320, 1053
MbiI	CCGCTC	1 cut(s)	441
MboI	GATC	4 cut(s)	214, 249, 318, 1051
MboII	GAAGA	5 cut(s)	256, 259, 518, 572, 1016
MhlI	GDGCHC	2 cut(s)	565, 994
MluCI	AATT	3 cut(s)	603, 857, 970
MluI	ACGCGT	1 cut(s)	53
MlyI	GAGTC	5 cut(s)	257, 291, 350, 492, 804
MmeI	TCCRAC	4 cut(s)	101, 252, 554, 919
MnlI	CCTC	9 cut(s)	264, 323, 455, 502, 583, 708, 899, 994, 1000
MseI	TTAA	3 cut(s)	513, 800, 854
MslI	CAYNNNNRTG	1 cut(s)	56
MspA1I	CMGCKG	1 cut(s)	1049
MspCI	CTTAAG	1 cut(s)	799
MspI	CCGG	3 cut(s)	199, 241, 303
MspR9I	CCNGG	3 cut(s)	241, 641, 662
MvaI	CCWGG	2 cut(s)	641, 662
MvnI	CGCG	3 cut(s)	55, 394, 432
MwoI	GCNNNNNNNGC	9 cut(s)	103, 112, 208, 280, 283, 379, 415, 438, 476
NciI	CCSGG	1 cut(s)	241
NcoI	CCATGG	2 cut(s)	377, 894
NdeII	GATC	4 cut(s)	214, 249, 318, 1051
NlaIII	CATG	5 cut(s)	381, 418, 469, 762, 898
NmeAIII	GCCGAG	1 cut(s)	505
NmuCI	GTSAC	2 cut(s)	58, 334
NsbI	TGCGCA	1 cut(s)	114
NspI	RCATGY	1 cut(s)	418
OliI	CACNNNNGTG	1 cut(s)	56
PaqCI	CACCTGC	1 cut(s)	111
PcsI	WCGNNNNNNNCGW	2 cut(s)	199, 483
PfeI	GAWTC	1 cut(s)	306
PflFI	GACNNNGTC	1 cut(s)	340
PflMI	CCANNNNNTGG	1 cut(s)	646
PfoI	TCCNGGA	2 cut(s)	639, 660
PkrI	GCNGC	8 cut(s)	99, 276, 285, 375, 396, 443, 495, 739
PleI	GAGTC	5 cut(s)	257, 291, 349, 492, 803
PmaCI	CACGTG	1 cut(s)	35
PmlI	CACGTG	1 cut(s)	35
PpsI	GAGTC	5 cut(s)	257, 291, 349, 492, 803
Ppu21I	YACGTR	1 cut(s)	35
Psp124BI	GAGCTC	1 cut(s)	994
Psp1406I	AACGTT	1 cut(s)	815
Psp6I	CCWGG	2 cut(s)	639, 660
PspCI	CACGTG	1 cut(s)	35
PspEI	GGTNACC	1 cut(s)	334
PspGI	CCWGG	2 cut(s)	639, 660
PspPI	GGNCC	2 cut(s)	238, 891
PstNI	CAGNNNCTG	1 cut(s)	289
PsyI	GACNNNGTC	1 cut(s)	340
PvuII	CAGCTG	1 cut(s)	1049
RsaI	GTAC	1 cut(s)	84
RsaNI	GTAC	1 cut(s)	83
RseI	CAYNNNNRTG	1 cut(s)	56
SacI	GAGCTC	1 cut(s)	994
SalI	GTCGAC	1 cut(s)	388
SaqAI	TTAA	3 cut(s)	513, 800, 854
SatI	GCNGC	8 cut(s)	98, 275, 284, 374, 395, 442, 494, 738
Sau3AI	GATC	4 cut(s)	214, 249, 318, 1051
Sau96I	GGNCC	2 cut(s)	238, 891
SchI	GAGTC	5 cut(s)	257, 291, 350, 492, 804
ScrFI	CCNGG	3 cut(s)	241, 641, 662
SduI	GDGCHC	2 cut(s)	565, 994
SfaNI	GCATC	2 cut(s)	857, 894
SfcI	CTRYAG	1 cut(s)	807
SgrDI	CGTCGACG	1 cut(s)	388
SinI	GGWCC	1 cut(s)	891
SmiMI	CAYNNNNRTG	1 cut(s)	56
SmlI	CTYRAG	2 cut(s)	278, 799
SmoI	CTYRAG	2 cut(s)	278, 799
Sse9I	AATT	3 cut(s)	603, 857, 970
SsiI	CCGC	5 cut(s)	98, 159, 394, 432, 441
SstI	GAGCTC	1 cut(s)	994
StyD4I	CCNGG	3 cut(s)	239, 639, 660
StyI	CCWWGG	3 cut(s)	377, 681, 894
TaaI	ACNGT	4 cut(s)	82, 692, 865, 937
TaiI	ACGT	2 cut(s)	37, 818
TaqI	TCGA	3 cut(s)	309, 389, 1035
TaqII	GACCGA	2 cut(s)	353, 548
TasI	AATT	3 cut(s)	603, 857, 970
TatI	WGTACW	1 cut(s)	82
TauI	GCSGC	3 cut(s)	100, 397, 444
TfiI	GAWTC	1 cut(s)	306
Tru1I	TTAA	3 cut(s)	513, 800, 854
Tru9I	TTAA	3 cut(s)	513, 800, 854
TscAI	CASTG	3 cut(s)	739, 786, 868
TseFI	GTSAC	2 cut(s)	58, 334
TseI	GCWGC	5 cut(s)	274, 283, 373, 493, 737
Tsp45I	GTSAC	2 cut(s)	58, 334
TspDTI	ATGAA	4 cut(s)	604, 683, 812, 849
TspGWI	ACGGA	1 cut(s)	362
TspRI	CASTG	3 cut(s)	739, 786, 868
Tth111I	GACNNNGTC	1 cut(s)	340
Van91I	CCANNNNNTGG	1 cut(s)	646
Vha464I	CTTAAG	1 cut(s)	799
VpaK11BI	GGWCC	1 cut(s)	891
XapI	RAATTY	1 cut(s)	857
XceI	RCATGY	1 cut(s)	418
XcmI	CCANNNNNNNNNTGG	1 cut(s)	583
XmiI	GTMKAC	1 cut(s)	389

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.