pycom16g12640

Nuclear export mediator factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
N/A
Physical Location & Seq
Reverse (-)
8881777 .. 8882798
1022 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 456 bp
ATGGTGAAGGAGGAGAAGAAGTATGGAAACCCGGTTGCTAGCCTCATTGACAAGCTTCAGCTTGAAAAGAATTGCATGACGTTGCTGCTGAGCAACAATCTTGATGAAATGGATGATGATGAGAAGACTCTCCCTGCAGATAAGGTGGAAGTTGATTTAACACTTTCAGCACATGCCAATGCTCGACGGTGGTATGAACTAAAGAAAAAACAGGAGAGCAAACAGGAGAAGACTGTAACAGCACATGAAAAGGCATTTAAGGCGGCTGAGAAAAAGACTTGTCTCCAGCTGTCACAGGAAAAAGCTGTTGCTAGCATTTCACATATGCGCAAGGTTCACTGGTTTGAGAAATTTAACTGGTTCATCAGCAGTGAGAACTACTTGATTATCAGTGGACGTGATGCTCAACAAAATGAGATGATAGTCAAGCGCTATATGTCAAAAGGATCGATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006810 GO:0006913 GO:0008150 GO:0046907 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051641 GO:0051649
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3441 DUF814 FbpA zf-CCHC

Protein Analysis

152

Amino Acids

17.54

Weight (kDa)

8.74

Isoelectric Point (pI)

39.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NFACT-R_1 PF05670 117 - 149 6.9e-10 NFACT protein RNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 329
AciI CCGC 1 cut(s) 263
AclWI GGATC 1 cut(s) 454
AcsI RAATTY 1 cut(s) 350
AcuI CTGAAG 1 cut(s) 41
AfeI AGCGCT 1 cut(s) 431
AgsI TTSAA 1 cut(s) 65
AjiI CACGTC 1 cut(s) 398
AluBI AGCT 4 cut(s) 55, 61, 289, 305
AluI AGCT 4 cut(s) 55, 61, 289, 305
Alw26I GTCTC 1 cut(s) 287
AlwI GGATC 1 cut(s) 454
Aor51HI AGCGCT 1 cut(s) 431
ApeKI GCWGC 1 cut(s) 85
ApoI RAATTY 1 cut(s) 350
AspLEI GCGC 2 cut(s) 330, 432
AsuC2I CCSGG 1 cut(s) 32
AsuHPI GGTGA 1 cut(s) 16
AsuNHI GCTAGC 2 cut(s) 38, 311
BbsI GAAGAC 2 cut(s) 131, 236
BbvI GCAGC 1 cut(s) 72
BcnI CCSGG 1 cut(s) 32
BcoDI GTCTC 1 cut(s) 287
BfaI CTAG 2 cut(s) 39, 312
BfmI CTRYAG 1 cut(s) 135
BfoI RGCGCY 1 cut(s) 433
BisI GCNGC 2 cut(s) 86, 264
BlpI GCTNAGC 1 cut(s) 89
BlsI GCNGC 2 cut(s) 87, 265
Bme1390I CCNGG 1 cut(s) 32
BmgBI CACGTC 1 cut(s) 398
BmrFI CCNGG 1 cut(s) 32
BmsI GCATC 1 cut(s) 391
BmtI GCTAGC 2 cut(s) 42, 315
BpiI GAAGAC 2 cut(s) 131, 236
BpmI CTGGAG 1 cut(s) 269
Bpu1102I GCTNAGC 1 cut(s) 89
BpuMI CCSGG 1 cut(s) 32
Bsa29I ATCGAT 1 cut(s) 449
BsaXI ACNNNNNCTCC 2 cut(s) 5, 35
Bse1I ACTGG 2 cut(s) 344, 362
BseCI ATCGAT 1 cut(s) 449
BseGI GGATG 1 cut(s) 118
BseMII CTCAG 2 cut(s) 80, 258
BseNI ACTGG 2 cut(s) 344, 362
BseRI GAGGAG 1 cut(s) 26
BseXI GCAGC 1 cut(s) 72
BshVI ATCGAT 1 cut(s) 449
BsiSI CCGG 1 cut(s) 32
BsmAI GTCTC 1 cut(s) 287
Bsp143I GATC 2 cut(s) 446, 450
Bsp1720I GCTNAGC 1 cut(s) 89
BspACI CCGC 1 cut(s) 263
BspCNI CTCAG 2 cut(s) 81, 259
BspDI ATCGAT 1 cut(s) 449
BspMAI CTGCAG 1 cut(s) 139
BspOI GCTAGC 2 cut(s) 42, 315
BspPI GGATC 1 cut(s) 454
BsrI ACTGG 2 cut(s) 344, 362
BssMI GATC 2 cut(s) 446, 450
Bst4CI ACNGT 2 cut(s) 189, 235
BstC8I GCNNGC 2 cut(s) 40, 313
BstDEI CTNAG 2 cut(s) 89, 267
BstF5I GGATG 1 cut(s) 118
BstH2I RGCGCY 1 cut(s) 433
BstHHI GCGC 2 cut(s) 330, 432
BstKTI GATC 2 cut(s) 449, 453
BstMAI GTCTC 1 cut(s) 287
BstMBI GATC 2 cut(s) 446, 450
BstMWI GCNNNNNNNGC 1 cut(s) 260
BstNSI RCATGY 1 cut(s) 176
BstSCI CCNGG 1 cut(s) 30
BstSFI CTRYAG 1 cut(s) 135
BstV1I GCAGC 1 cut(s) 72
BstV2I GAAGAC 2 cut(s) 131, 236
Bsu15I ATCGAT 1 cut(s) 449
BsuTUI ATCGAT 1 cut(s) 449
BtrI CACGTC 1 cut(s) 398
BtsCI GGATG 1 cut(s) 118
BtsI GCAGTG 1 cut(s) 376
BtsIMutI CAGTG 3 cut(s) 337, 376, 397
Cac8I GCNNGC 2 cut(s) 40, 313
CfoI GCGC 2 cut(s) 330, 432
ClaI ATCGAT 1 cut(s) 449
CviAII CATG 3 cut(s) 76, 173, 245
CviJI RGCY 6 cut(s) 42, 55, 61, 266, 289, 305
CviKI_1 RGCY 6 cut(s) 42, 55, 61, 266, 289, 305
DdeI CTNAG 2 cut(s) 89, 267
DpnI GATC 2 cut(s) 448, 452
DpnII GATC 2 cut(s) 446, 450
Eco47III AGCGCT 1 cut(s) 431
Eco57I CTGAAG 1 cut(s) 41
FaeI CATG 3 cut(s) 79, 176, 248
FaiI YATR 9 cut(s) 24, 77, 174, 195, 246, 324, 326, 435, 437
FatI CATG 3 cut(s) 75, 172, 244
FauNDI CATATG 1 cut(s) 324
Fnu4HI GCNGC 2 cut(s) 86, 264
FokI GGATG 1 cut(s) 125
Fsp4HI GCNGC 2 cut(s) 86, 264
FspBI CTAG 2 cut(s) 39, 312
FspI TGCGCA 1 cut(s) 329
GlaI GCGC 2 cut(s) 329, 431
GluI GCNGC 2 cut(s) 86, 264
GsuI CTGGAG 1 cut(s) 269
HaeII RGCGCY 1 cut(s) 433
HapII CCGG 1 cut(s) 32
HhaI GCGC 2 cut(s) 330, 432
Hin1II CATG 3 cut(s) 79, 176, 248
Hin6I GCGC 2 cut(s) 328, 430
HinP1I GCGC 2 cut(s) 328, 430
HindIII AAGCTT 1 cut(s) 53
HinfI GANTC 1 cut(s) 127
HpaII CCGG 1 cut(s) 32
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 2 cut(s) 337, 395
Hpy188I TCNGA 1 cut(s) 455
Hpy188III TCNNGA 1 cut(s) 101
Hpy8I GTNNAC 2 cut(s) 337, 395
Hpy99I CGWCG 1 cut(s) 189
HpyCH4III ACNGT 2 cut(s) 189, 235
HpyCH4IV ACGT 2 cut(s) 80, 397
HpyCH4V TGCA 2 cut(s) 75, 137
HpyF10VI GCNNNNNNNGC 1 cut(s) 260
HpyF3I CTNAG 2 cut(s) 89, 267
HpySE526I ACGT 2 cut(s) 80, 397
Hsp92II CATG 3 cut(s) 79, 176, 248
HspAI GCGC 2 cut(s) 328, 430
Kzo9I GATC 2 cut(s) 446, 450
LpnPI CCDG 8 cut(s) 45, 147, 197, 209, 281, 299, 325, 343
Lsp1109I GCAGC 1 cut(s) 72
LweI GCATC 1 cut(s) 391
MaeI CTAG 2 cut(s) 39, 312
MaeII ACGT 2 cut(s) 80, 397
MaeIII GTNAC 2 cut(s) 235, 291
MalI GATC 2 cut(s) 448, 452
MboI GATC 2 cut(s) 446, 450
MboII GAAGA 3 cut(s) 28, 136, 241
MluCI AATT 2 cut(s) 70, 350
MlyI GAGTC 1 cut(s) 121
MnlI CCTC 2 cut(s) 4, 53
MseI TTAA 3 cut(s) 158, 258, 354
MslI CAYNNNNRTG 1 cut(s) 177
MspA1I CMGCKG 1 cut(s) 289
MspI CCGG 1 cut(s) 32
MspR9I CCNGG 1 cut(s) 32
MwoI GCNNNNNNNGC 1 cut(s) 260
NciI CCSGG 1 cut(s) 32
NdeI CATATG 1 cut(s) 324
NdeII GATC 2 cut(s) 446, 450
NheI GCTAGC 2 cut(s) 38, 311
NlaIII CATG 3 cut(s) 79, 176, 248
NmuCI GTSAC 1 cut(s) 291
NsbI TGCGCA 1 cut(s) 329
NspI RCATGY 1 cut(s) 176
PkrI GCNGC 2 cut(s) 87, 265
PleI GAGTC 1 cut(s) 121
PpsI GAGTC 1 cut(s) 121
PstI CTGCAG 1 cut(s) 139
PvuII CAGCTG 1 cut(s) 289
RseI CAYNNNNRTG 1 cut(s) 177
SaqAI TTAA 3 cut(s) 158, 258, 354
SatI GCNGC 2 cut(s) 86, 264
Sau3AI GATC 2 cut(s) 446, 450
SchI GAGTC 1 cut(s) 121
ScrFI CCNGG 1 cut(s) 32
SetI ASST 8 cut(s) 57, 63, 83, 147, 291, 307, 336, 400
SfaNI GCATC 1 cut(s) 391
SfcI CTRYAG 1 cut(s) 135
SmiMI CAYNNNNRTG 1 cut(s) 177
Sse9I AATT 2 cut(s) 70, 350
SsiI CCGC 1 cut(s) 263
SspMI CTAG 2 cut(s) 39, 312
StyD4I CCNGG 1 cut(s) 30
TaaI ACNGT 2 cut(s) 189, 235
TaiI ACGT 2 cut(s) 83, 400
TaqI TCGA 2 cut(s) 184, 449
TasI AATT 2 cut(s) 70, 350
TauI GCSGC 1 cut(s) 266
Tru1I TTAA 3 cut(s) 158, 258, 354
Tru9I TTAA 3 cut(s) 158, 258, 354
TscAI CASTG 3 cut(s) 344, 376, 397
TseFI GTSAC 1 cut(s) 291
TseI GCWGC 1 cut(s) 85
Tsp45I GTSAC 1 cut(s) 291
TspDTI ATGAA 4 cut(s) 120, 210, 261, 352
TspRI CASTG 3 cut(s) 344, 376, 397
XapI RAATTY 1 cut(s) 350
XceI RCATGY 1 cut(s) 176
XspI CTAG 2 cut(s) 39, 312
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.