pycom16g13510

Major allergen Pru

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
N/A
Physical Location & Seq
Reverse (-)
9697910 .. 9698563
654 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 543 bp
ATGGGTGTTTTCACATATGAAACCGAGTTCACCTCTGTCATCCCTGCACCTAGATTGTTCAAGGCTTTTATCCTCGATGGTGATAACCTCATCCCGAAGATTGCTCCACAAGCAATTAAAAGCACTGAAATCATCGAAGGCGATGGAGGTGTTGGAACCATCAAGAAAGTTACCTTTGGTGAAGGCAGCCAATACGGGTATGTTAAGCAAAGGATCAATGGGATTGACAAAGACAACTTTACATACAGTTACTCTATGATTGAAGGAGATACCTTGTCTGATAAGCTTGAGAAGATCACTTATGAGACCAAATTGATCGCATCACCTAATGGAGGATCCGTCATCAAAACCACTAGCCACTACCATGCCAAGGGTGATGTTGATCGATCAAGGAAGAGCATATTAAAGCTGGCAAAGAGAAGGCTTCCGGTCTCTTCAAGCTCCTTGAAGCCTACCTCGTGGCCAACCCTGATGCCTACAACTAAATCTGATGTTATCCATATATGTAATTTGTGTGTTACCTTTGTTCTCTTCATGGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bet_v_1

Protein Analysis

181

Amino Acids

19.85

Weight (kDa)

9.13

Isoelectric Point (pI)

36.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bet_v_1 PF00407 1 - 138 3.9e-23 Pathogenesis-related protein Bet v 1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 221, 330, 343
AcoI YGGCCR 1 cut(s) 461
AfiI CCNNNNNNNGG 2 cut(s) 332, 370
AgsI TTSAA 4 cut(s) 61, 263, 438, 448
AluBI AGCT 3 cut(s) 286, 409, 441
AluI AGCT 3 cut(s) 286, 409, 441
Alw26I GTCTC 2 cut(s) 299, 436
AlwI GGATC 3 cut(s) 221, 330, 343
AoxI GGCC 1 cut(s) 461
ApeKI GCWGC 1 cut(s) 186
AsuHPI GGTGA 5 cut(s) 22, 92, 191, 315, 386
BalI TGGCCA 1 cut(s) 463
BamHI GGATCC 1 cut(s) 335
BauI CACGAG 1 cut(s) 457
BbvI GCAGC 1 cut(s) 198
BccI CCATC 3 cut(s) 71, 137, 167
BcoDI GTCTC 2 cut(s) 299, 436
BfaI CTAG 2 cut(s) 51, 354
BisI GCNGC 1 cut(s) 187
BlsI GCNGC 1 cut(s) 188
BmiI GGNNCC 2 cut(s) 157, 337
BmsI GCATC 2 cut(s) 329, 462
BplI GAGNNNNNCTC 2 cut(s) 17, 49
BpuEI CTTGAG 1 cut(s) 308
Bsa29I ATCGAT 1 cut(s) 385
BsaBI GATNNNNATC 1 cut(s) 381
BsaI GGTCTC 2 cut(s) 299, 436
BsaJI CCNNGG 1 cut(s) 369
BsaWI WCCGGW 1 cut(s) 427
BsaXI ACNNNNNCTCC 2 cut(s) 324, 354
Bsc4I CCNNNNNNNGG 2 cut(s) 332, 370
Bse8I GATNNNNATC 1 cut(s) 381
BseCI ATCGAT 1 cut(s) 385
BseDI CCNNGG 1 cut(s) 369
BseGI GGATG 2 cut(s) 39, 90
BseJI GATNNNNATC 1 cut(s) 381
BseLI CCNNNNNNNGG 2 cut(s) 332, 370
BseXI GCAGC 1 cut(s) 198
BsgI GTGCAG 1 cut(s) 30
BshFI GGCC 1 cut(s) 463
BshVI ATCGAT 1 cut(s) 385
BsiSI CCGG 1 cut(s) 428
BslI CCNNNNNNNGG 2 cut(s) 332, 370
BsmAI GTCTC 2 cut(s) 299, 436
BsnI GGCC 1 cut(s) 463
Bso31I GGTCTC 2 cut(s) 299, 436
Bsp143I GATC 6 cut(s) 213, 294, 315, 335, 382, 386
BspANI GGCC 1 cut(s) 463
BspDI ATCGAT 1 cut(s) 385
BspLI GGNNCC 2 cut(s) 157, 337
BspPI GGATC 3 cut(s) 221, 330, 343
BspQI GCTCTTC 1 cut(s) 389
BspTNI GGTCTC 2 cut(s) 299, 436
BssECI CCNNGG 1 cut(s) 369
BssMI GATC 6 cut(s) 213, 294, 315, 335, 382, 386
BssSI CACGAG 1 cut(s) 457
BssT1I CCWWGG 1 cut(s) 369
Bst2BI CACGAG 1 cut(s) 457
Bst4CI ACNGT 1 cut(s) 248
Bst6I CTCTTC 3 cut(s) 389, 439, 536
BstC8I GCNNGC 1 cut(s) 411
BstENI CCTNNNNNAGG 1 cut(s) 330
BstF5I GGATG 2 cut(s) 39, 90
BstKTI GATC 6 cut(s) 216, 297, 318, 338, 385, 389
BstMAI GTCTC 2 cut(s) 299, 436
BstMBI GATC 6 cut(s) 213, 294, 315, 335, 382, 386
BstMWI GCNNNNNNNGC 1 cut(s) 110
BstV1I GCAGC 1 cut(s) 198
BstX2I RGATCY 1 cut(s) 335
BstYI RGATCY 1 cut(s) 335
Bsu15I ATCGAT 1 cut(s) 385
BsuRI GGCC 1 cut(s) 463
BsuTUI ATCGAT 1 cut(s) 385
BtgZI GCGATG 1 cut(s) 156
BtsCI GGATG 2 cut(s) 39, 90
BtsIMutI CAGTG 1 cut(s) 123
Cac8I GCNNGC 1 cut(s) 411
ClaI ATCGAT 1 cut(s) 385
CviAII CATG 2 cut(s) 365, 535
CviJI RGCY 9 cut(s) 65, 189, 286, 357, 409, 424, 441, 451, 463
CviKI_1 RGCY 9 cut(s) 65, 189, 286, 357, 409, 424, 441, 451, 463
DpnI GATC 6 cut(s) 215, 296, 317, 337, 384, 388
DpnII GATC 6 cut(s) 213, 294, 315, 335, 382, 386
EaeI YGGCCR 1 cut(s) 461
Eam1104I CTCTTC 3 cut(s) 389, 439, 536
EarI CTCTTC 3 cut(s) 389, 439, 536
Eco130I CCWWGG 1 cut(s) 369
Eco31I GGTCTC 2 cut(s) 299, 436
EcoNI CCTNNNNNAGG 1 cut(s) 330
EcoT14I CCWWGG 1 cut(s) 369
ErhI CCWWGG 1 cut(s) 369
FaeI CATG 2 cut(s) 368, 538
FatI CATG 2 cut(s) 364, 534
FauNDI CATATG 1 cut(s) 16
Fnu4HI GCNGC 1 cut(s) 187
FokI GGATG 2 cut(s) 26, 77
Fsp4HI GCNGC 1 cut(s) 187
FspBI CTAG 2 cut(s) 51, 354
GluI GCNGC 1 cut(s) 187
HaeIII GGCC 1 cut(s) 463
HapII CCGG 1 cut(s) 428
Hin1II CATG 2 cut(s) 368, 538
HindIII AAGCTT 1 cut(s) 284
HpaII CCGG 1 cut(s) 428
HphI GGTGA 5 cut(s) 22, 92, 191, 315, 386
Hpy166II GTNNAC 1 cut(s) 30
Hpy188I TCNGA 2 cut(s) 280, 490
Hpy188III TCNNGA 2 cut(s) 94, 163
Hpy8I GTNNAC 1 cut(s) 30
HpyAV CCTTC 4 cut(s) 131, 176, 257, 414
HpyCH4III ACNGT 1 cut(s) 248
HpyCH4V TGCA 1 cut(s) 47
HpyF10VI GCNNNNNNNGC 1 cut(s) 110
Hsp92II CATG 2 cut(s) 368, 538
Kzo9I GATC 6 cut(s) 213, 294, 315, 335, 382, 386
LguI GCTCTTC 1 cut(s) 389
LmnI GCTCC 2 cut(s) 109, 446
LpnPI CCDG 4 cut(s) 57, 395, 441, 482
Lsp1109I GCAGC 1 cut(s) 198
LweI GCATC 2 cut(s) 329, 462
MaeI CTAG 2 cut(s) 51, 354
MaeIII GTNAC 3 cut(s) 169, 248, 517
MalI GATC 6 cut(s) 215, 296, 317, 337, 384, 388
MboI GATC 6 cut(s) 213, 294, 315, 335, 382, 386
MboII GAAGA 5 cut(s) 109, 304, 406, 426, 523
MflI RGATCY 1 cut(s) 335
MlsI TGGCCA 1 cut(s) 463
MluCI AATT 3 cut(s) 114, 311, 508
MluNI TGGCCA 1 cut(s) 463
MmeI TCCRAC 1 cut(s) 133
MnlI CCTC 6 cut(s) 43, 83, 98, 140, 326, 466
Mox20I TGGCCA 1 cut(s) 463
MscI TGGCCA 1 cut(s) 463
MseI TTAA 3 cut(s) 117, 204, 404
MslI CAYNNNNRTG 1 cut(s) 363
Msp20I TGGCCA 1 cut(s) 463
MspI CCGG 1 cut(s) 428
MwoI GCNNNNNNNGC 1 cut(s) 110
NdeI CATATG 1 cut(s) 16
NdeII GATC 6 cut(s) 213, 294, 315, 335, 382, 386
NlaIII CATG 2 cut(s) 368, 538
NlaIV GGNNCC 2 cut(s) 157, 337
PciSI GCTCTTC 1 cut(s) 389
PkrI GCNGC 1 cut(s) 188
PspN4I GGNNCC 2 cut(s) 157, 337
PsuI RGATCY 1 cut(s) 335
RseI CAYNNNNRTG 1 cut(s) 363
SapI GCTCTTC 1 cut(s) 389
SaqAI TTAA 3 cut(s) 117, 204, 404
SatI GCNGC 1 cut(s) 187
Sau3AI GATC 6 cut(s) 213, 294, 315, 335, 382, 386
SfaNI GCATC 2 cut(s) 329, 462
SmiMI CAYNNNNRTG 1 cut(s) 363
SmlI CTYRAG 1 cut(s) 287
SmoI CTYRAG 1 cut(s) 287
Sse9I AATT 3 cut(s) 114, 311, 508
SspMI CTAG 2 cut(s) 51, 354
StyI CCWWGG 1 cut(s) 369
TaaI ACNGT 1 cut(s) 248
TaqI TCGA 3 cut(s) 75, 135, 385
TasI AATT 3 cut(s) 114, 311, 508
Tru1I TTAA 3 cut(s) 117, 204, 404
Tru9I TTAA 3 cut(s) 117, 204, 404
TscAI CASTG 1 cut(s) 130
TseI GCWGC 1 cut(s) 186
TspDTI ATGAA 2 cut(s) 33, 523
TspGWI ACGGA 1 cut(s) 328
TspRI CASTG 1 cut(s) 130
XagI CCTNNNNNAGG 1 cut(s) 330
XspI CTAG 2 cut(s) 51, 354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.