pycom16g13620

Major allergen Pru ar 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
N/A
Physical Location & Seq
Forward (+)
9744420 .. 9747630
3211 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1998 bp
ATGTCTCAATGTGGTCGTGAAAAAAAAACTGTATGTATGAACTTATGGATATCCTTAACCAAATTAAGATGTGTTATTTGGTCATTTGCTAACTATTTTGTTTTGTTTTGTTTTATTTTATTTTTTTTGAAAATTGAAAACTTGTTTATGAAAGTTACTTTTATGAGATTTCAAATTTTGACTTGGCTTTTAATGTCATTTATTCGAAACCAATTTATCTCATCACTAAGTGTAAATATATATAGTGTGTTTTCTTTTACAAATGATAGCGTTAGTGAATTAAGTTTGATTTTTGTCATAGTGGTAAAACACGGTATGTGTAAATATTTTCGTTATTTAAAAAAAACAAAAAATTTGGCAGGCAATATTCAAAGTATAAGAGGCAGCTGGAAACGCCTCATGCGTTGTGTCATTTCAATAAGGTATCCCAAGGCACAAATTTGTTCTTCGAAAAAAAAAAAATTTCTTTTCTTTTGTCTTTCCTGTAGCGGATTGGGAAATCGACCTTGGAAATACCGCCCCTTATATTATCCCCCTGACCTTTTTTTTCCAAAAACAAGCCATTTGCTTATTCATTTCATTTTTGTAAATTCCAGCATGGGTGTCTTCACTCGTACAGATGAGTACACTTCCCCAATCCCTCCGGACAGGTTGTTCAAGGCCTTGGTTCTTGATGCTCACATCCTGATTCCAGAGCTCATGCCAGAGGCTATTAAGAGCATTGACAACCTCAAGGGTGACGGAAGAGCAGGAAGCATTAAGAAGATCAACTACGCTGAAGGTAGCCAGCTCAAATCTGTGATAAACCGGGTTGACGACGTCGACGAAGAGAACTTTGTATATGCTTACACTTTGGTTGAAGGAGAACCATTAGTAGTGGAGAAACTTGAATACATCACTTATAAGGCTAAGTTTGAAGCTGCATCAGATGGGGGTAGTAAGAACCGGTTGGTAAGCAATTACTACACCAAGGGTGACGTTGTGCTGAAAGAGGAAGAAATAAAAGCCGGCAGGGAAAGGGCCTTGGGGATGTACAGAGTTGTGGAAGCCTATCTCCTCCAAAATCCTGATGCCTATGGAAATATGCTTGACAATGCCTGTAACTATGGCCATTGGAATATACCTTCTCCATGTATTGACTTCAATAAAAATAATTTTTCCATGATTTTGACACACTCATTTTCATCTCTTACAAACTTTCTGTTAATTTCCAGAATAACATGGGTGCGGGAAAATTATTACCGTTTTCTTCTTATTTATTTATTATTTATAAAGGGGCCACAATCTCCCAATAACCAATCCATGACTGATGGGGTCAGGAATGGCCATTTGACCCATGGGTACTTAAATGAATTAGATGGGTTTGGGTATTTTGTTGAGATCAAATCTTCTGCATCCAAAATGAGTATGGTTTTTTTACGTGTCAATCATTTATTAAAGTCACAAAAAGATCATACCTATTTTGAGTGCACAAAATTGTCTCCTTTTTGTTGGGGGTTTCTCCTCCATGTCTGCTTCCTCTTTTCTTCATCGTCGTCTCTGTCTGTCTCCTTCACCGACACCGGCAAACCTTCCATAGCTGGCGTCTCATATCCACCTCCTCCTTTGCCCTTTTCTGTACCTTTCTCTGTCTCTTTCTCTTTTGTTTTTTGGTTTTTCGGTTTTTGCTTTTTTTTTTTTGTTTTTTTGTTTTGTGTCTTGCTCTCTCTAGCATTTCAGATCCGCAAGGAAGGGATTAATGTCAACCAAAATTTTAGGTCATCTTTTGTAGACCACATGAATCTAACCATCAATCGCCACATCATTTTTTACACAGTATTTTATAATGTTAGCACTGGAATAAGAGAAATGCTAAGGGATACTCTCAAAAATATTAGTAGTTGGAAATTATTTTTTACACAACTCTCAAAAGTGAGACTCTCCATGTACTCTCTACCACCTCATGTACTCTCTAGCACTGGAATAGGAGAAATGCTAAGGCTGAAAACCATGTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bet_v_1

Protein Analysis

666

Amino Acids

76.93

Weight (kDa)

9.4

Isoelectric Point (pI)

35.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bet_v_1 PF00407 200 - 354 2.1e-16 Pathogenesis-related protein Bet v 1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 903, 1271, 1824
AatII GACGTC 1 cut(s) 822
AccI GTMKAC 2 cut(s) 822, 1770
AccIII TCCGGA 1 cut(s) 643
AciI CCGC 4 cut(s) 489, 517, 1228, 1723
AclWI GGATC 1 cut(s) 1714
AcoI YGGCCR 2 cut(s) 1108, 1324
AcsI RAATTY 6 cut(s) 174, 352, 438, 461, 589, 1750
AcuI CTGAAG 1 cut(s) 798
AcyI GRCGYC 2 cut(s) 819, 1584
AdeI CACNNNGTG 1 cut(s) 230
AfaI GTAC 7 cut(s) 616, 626, 1034, 1343, 1620, 1928, 1947
AflIII ACRYGT 1 cut(s) 1420
AgeI ACCGGT 1 cut(s) 945
AjuI GAANNNNNNNTTGG 2 cut(s) 490, 522
AloI GAACNNNNNNTCC 2 cut(s) 638, 670
AluBI AGCT 5 cut(s) 387, 697, 790, 920, 1580
AluI AGCT 5 cut(s) 387, 697, 790, 920, 1580
Alw21I GWGCWC 2 cut(s) 699, 1472
Alw26I GTCTC 7 cut(s) 9, 1485, 1542, 1552, 1591, 1636, 1909
Alw44I GTGCAC 1 cut(s) 1468
AlwI GGATC 1 cut(s) 1714
Aor13HI TCCGGA 1 cut(s) 643
AoxI GGCC 5 cut(s) 660, 1020, 1108, 1277, 1324
ApaLI GTGCAC 1 cut(s) 1468
ApeKI GCWGC 2 cut(s) 384, 920
ApoI RAATTY 6 cut(s) 174, 352, 438, 461, 589, 1750
AseI ATTAAT 1 cut(s) 1737
AsiGI ACCGGT 1 cut(s) 945
AspS9I GGNCC 2 cut(s) 1020, 1277
AsuC2I CCSGG 1 cut(s) 809
AsuHPI GGTGA 3 cut(s) 749, 986, 1546
AsuII TTCGAA 2 cut(s) 205, 449
BaeGI GKGCMC 1 cut(s) 1472
BalI TGGCCA 2 cut(s) 1110, 1326
BanII GRGCYC 1 cut(s) 699
BarI GAAGNNNNNNTAC 2 cut(s) 755, 787
BbsI GAAGAC 1 cut(s) 598
Bbv12I GWGCWC 2 cut(s) 699, 1472
BbvI GCAGC 2 cut(s) 396, 907
BccI CCATC 4 cut(s) 923, 1304, 1352, 1796
BciVI GTATCC 2 cut(s) 435, 1852
BcnI CCSGG 1 cut(s) 809
BcoDI GTCTC 7 cut(s) 9, 1485, 1542, 1552, 1591, 1636, 1909
BfaI CTAG 2 cut(s) 1709, 1953
BfmI CTRYAG 1 cut(s) 484
BfuI GTATCC 2 cut(s) 435, 1852
BisI GCNGC 2 cut(s) 385, 921
BlsI GCNGC 2 cut(s) 386, 922
Bme1390I CCNGG 1 cut(s) 809
BmgT120I GGNCC 2 cut(s) 1020, 1277
BmiI GGNNCC 1 cut(s) 1278
BmrFI CCNGG 1 cut(s) 809
BmsI GCATC 4 cut(s) 664, 932, 1060, 1403
BpiI GAAGAC 1 cut(s) 598
Bpu10I CCTNAGC 2 cut(s) 1853, 1976
Bpu14I TTCGAA 2 cut(s) 205, 449
BpuEI CTTGAG 1 cut(s) 716
BpuMI CCSGG 1 cut(s) 809
BsaAI YACGTR 1 cut(s) 1421
BsaHI GRCGYC 2 cut(s) 819, 1584
BsaJI CCNNGG 6 cut(s) 429, 506, 663, 969, 1023, 1336
BsaWI WCCGGW 2 cut(s) 643, 945
Bse118I RCCGGY 3 cut(s) 945, 1007, 1562
Bse1I ACTGG 2 cut(s) 1840, 1963
BseAI TCCGGA 1 cut(s) 643
BseDI CCNNGG 6 cut(s) 429, 506, 663, 969, 1023, 1336
BseGI GGATG 3 cut(s) 681, 1035, 1394
BseNI ACTGG 2 cut(s) 1840, 1963
BseRI GAGGAG 3 cut(s) 1046, 1493, 1590
BseSI GKGCMC 1 cut(s) 1472
BseXI GCAGC 2 cut(s) 396, 907
BshFI GGCC 5 cut(s) 662, 1022, 1110, 1279, 1326
BshTI ACCGGT 1 cut(s) 945
BsiHKAI GWGCWC 2 cut(s) 699, 1472
BsiSI CCGG 5 cut(s) 644, 808, 946, 1008, 1563
BsmAI GTCTC 7 cut(s) 9, 1485, 1542, 1552, 1591, 1636, 1909
BsmBI CGTCTC 2 cut(s) 1542, 1591
BsnI GGCC 5 cut(s) 662, 1022, 1110, 1279, 1326
Bsp119I TTCGAA 2 cut(s) 205, 449
Bsp1286I GDGCHC 2 cut(s) 699, 1472
Bsp13I TCCGGA 1 cut(s) 643
Bsp1407I TGTACA 1 cut(s) 1032
Bsp143I GATC 4 cut(s) 765, 1380, 1450, 1719
Bsp19I CCATGG 1 cut(s) 1336
BspACI CCGC 4 cut(s) 489, 517, 1228, 1723
BspANI GGCC 5 cut(s) 662, 1022, 1110, 1279, 1326
BspEI TCCGGA 1 cut(s) 643
BspLI GGNNCC 1 cut(s) 1278
BspPI GGATC 1 cut(s) 1714
BspQI GCTCTTC 1 cut(s) 739
BspT104I TTCGAA 2 cut(s) 205, 449
BsrFI RCCGGY 3 cut(s) 945, 1007, 1562
BsrGI TGTACA 1 cut(s) 1032
BsrI ACTGG 2 cut(s) 1840, 1963
BssAI RCCGGY 3 cut(s) 945, 1007, 1562
BssECI CCNNGG 6 cut(s) 429, 506, 663, 969, 1023, 1336
BssMI GATC 4 cut(s) 765, 1380, 1450, 1719
BssNI GRCGYC 2 cut(s) 819, 1584
BssT1I CCWWGG 6 cut(s) 429, 506, 663, 969, 1023, 1336
Bst4CI ACNGT 4 cut(s) 31, 314, 1244, 1816
Bst6I CTCTTC 2 cut(s) 739, 822
BstACI GRCGYC 2 cut(s) 819, 1584
BstAUI TGTACA 1 cut(s) 1032
BstBAI YACGTR 1 cut(s) 1421
BstBI TTCGAA 2 cut(s) 205, 449
BstC8I GCNNGC 4 cut(s) 361, 788, 1009, 1582
BstDEI CTNAG 4 cut(s) 227, 909, 1853, 1976
BstDSI CCRYGG 1 cut(s) 1336
BstF5I GGATG 3 cut(s) 681, 1035, 1394
BstKTI GATC 4 cut(s) 768, 1383, 1453, 1722
BstMAI GTCTC 7 cut(s) 9, 1485, 1542, 1552, 1591, 1636, 1909
BstMBI GATC 4 cut(s) 765, 1380, 1450, 1719
BstMWI GCNNNNNNNGC 1 cut(s) 393
BstSCI CCNGG 1 cut(s) 807
BstSFI CTRYAG 1 cut(s) 484
BstSLI GKGCMC 1 cut(s) 1472
BstV1I GCAGC 2 cut(s) 396, 907
BstV2I GAAGAC 1 cut(s) 598
BstX2I RGATCY 1 cut(s) 1719
BstYI RGATCY 1 cut(s) 1719
BsuI GTATCC 2 cut(s) 435, 1852
BsuRI GGCC 5 cut(s) 662, 1022, 1110, 1279, 1326
BtgI CCRYGG 1 cut(s) 1336
BtsCI GGATG 3 cut(s) 681, 1035, 1394
BtsIMutI CAGTG 2 cut(s) 1833, 1956
Cac8I GCNNGC 4 cut(s) 361, 788, 1009, 1582
Cfr10I RCCGGY 3 cut(s) 945, 1007, 1562
Cfr13I GGNCC 2 cut(s) 1020, 1277
CseI GACGC 1 cut(s) 1573
Csp6I GTAC 7 cut(s) 615, 625, 1033, 1342, 1619, 1927, 1946
CspAI ACCGGT 1 cut(s) 945
CviQI GTAC 7 cut(s) 615, 625, 1033, 1342, 1619, 1927, 1946
DdeI CTNAG 4 cut(s) 227, 909, 1853, 1976
DpnI GATC 4 cut(s) 767, 1382, 1452, 1721
DpnII GATC 4 cut(s) 765, 1380, 1450, 1719
DraI TTTAAA 1 cut(s) 339
DraIII CACNNNGTG 1 cut(s) 230
EaeI YGGCCR 2 cut(s) 1108, 1324
Eam1104I CTCTTC 2 cut(s) 739, 822
EarI CTCTTC 2 cut(s) 739, 822
Ecl136II GAGCTC 1 cut(s) 697
Eco130I CCWWGG 6 cut(s) 429, 506, 663, 969, 1023, 1336
Eco147I AGGCCT 1 cut(s) 662
Eco24I GRGCYC 1 cut(s) 699
Eco32I GATATC 1 cut(s) 51
Eco53kI GAGCTC 1 cut(s) 697
Eco57I CTGAAG 1 cut(s) 798
EcoICRI GAGCTC 1 cut(s) 697
EcoO109I RGGNCCY 1 cut(s) 1020
EcoRV GATATC 1 cut(s) 51
EcoT14I CCWWGG 6 cut(s) 429, 506, 663, 969, 1023, 1336
EcoT38I GRGCYC 1 cut(s) 699
ErhI CCWWGG 6 cut(s) 429, 506, 663, 969, 1023, 1336
Esp3I CGTCTC 2 cut(s) 1542, 1591
FauI CCCGC 1 cut(s) 1221
FblI GTMKAC 2 cut(s) 822, 1770
Fnu4HI GCNGC 2 cut(s) 385, 921
FokI GGATG 3 cut(s) 668, 1042, 1381
FriOI GRGCYC 1 cut(s) 699
Fsp4HI GCNGC 2 cut(s) 385, 921
FspBI CTAG 2 cut(s) 1709, 1953
GluI GCNGC 2 cut(s) 385, 921
HaeIII GGCC 5 cut(s) 662, 1022, 1110, 1279, 1326
HapII CCGG 5 cut(s) 644, 808, 946, 1008, 1563
HgaI GACGC 1 cut(s) 1573
Hin1I GRCGYC 2 cut(s) 819, 1584
HincII GTYRAC 3 cut(s) 814, 823, 1744
HindII GTYRAC 3 cut(s) 814, 823, 1744
HinfI GANTC 3 cut(s) 688, 1780, 1917
HpaII CCGG 5 cut(s) 644, 808, 946, 1008, 1563
HphI GGTGA 3 cut(s) 749, 986, 1546
Hpy166II GTNNAC 6 cut(s) 627, 814, 823, 1470, 1744, 1771
Hpy188I TCNGA 2 cut(s) 928, 1719
Hpy188III TCNNGA 8 cut(s) 17, 644, 671, 685, 692, 1067, 1212, 1318
Hpy8I GTNNAC 6 cut(s) 627, 814, 823, 1470, 1744, 1771
Hpy99I CGWCG 4 cut(s) 821, 824, 827, 1537
HpyAV CCTTC 6 cut(s) 773, 854, 1134, 1561, 1581, 1724
HpyCH4III ACNGT 4 cut(s) 31, 314, 1244, 1816
HpyCH4IV ACGT 3 cut(s) 819, 978, 1420
HpyCH4V TGCA 3 cut(s) 923, 1394, 1470
HpyF10VI GCNNNNNNNGC 1 cut(s) 393
HpyF3I CTNAG 4 cut(s) 227, 909, 1853, 1976
HpySE526I ACGT 3 cut(s) 819, 978, 1420
Hsp92I GRCGYC 2 cut(s) 819, 1584
Kpn2I TCCGGA 1 cut(s) 643
KroI GCCGGC 1 cut(s) 1007
KroNI GCCGGC 1 cut(s) 1009
Kzo9I GATC 4 cut(s) 765, 1380, 1450, 1719
LguI GCTCTTC 1 cut(s) 739
Lsp1109I GCAGC 2 cut(s) 396, 907
LweI GCATC 4 cut(s) 664, 932, 1060, 1403
MaeI CTAG 2 cut(s) 1709, 1953
MaeII ACGT 3 cut(s) 819, 978, 1420
MaeIII GTNAC 5 cut(s) 154, 737, 974, 1100, 1440
MalI GATC 4 cut(s) 767, 1382, 1452, 1721
MboI GATC 4 cut(s) 765, 1380, 1450, 1719
MboII GAAGA 9 cut(s) 438, 598, 756, 775, 839, 1007, 1241, 1380, 1518
MflI RGATCY 1 cut(s) 1719
MhlI GDGCHC 2 cut(s) 699, 1472
MlsI TGGCCA 2 cut(s) 1110, 1326
MluNI TGGCCA 2 cut(s) 1110, 1326
MlyI GAGTC 1 cut(s) 1911
MmeI TCCRAC 1 cut(s) 1862
Mox20I TGGCCA 2 cut(s) 1110, 1326
MroI TCCGGA 1 cut(s) 643
MroNI GCCGGC 1 cut(s) 1007
MscI TGGCCA 2 cut(s) 1110, 1326
Msp20I TGGCCA 2 cut(s) 1110, 1326
MspA1I CMGCKG 1 cut(s) 387
MspI CCGG 5 cut(s) 644, 808, 946, 1008, 1563
MspR9I CCNGG 1 cut(s) 809
MwoI GCNNNNNNNGC 1 cut(s) 393
NaeI GCCGGC 1 cut(s) 1009
NciI CCSGG 1 cut(s) 809
NcoI CCATGG 1 cut(s) 1336
NdeII GATC 4 cut(s) 765, 1380, 1450, 1719
NgoMIV GCCGGC 1 cut(s) 1007
NlaIV GGNNCC 1 cut(s) 1278
NmuCI GTSAC 3 cut(s) 737, 974, 1440
NspV TTCGAA 2 cut(s) 205, 449
PceI AGGCCT 1 cut(s) 662
PciSI GCTCTTC 1 cut(s) 739
PcsI WCGNNNNNNNCGW 2 cut(s) 400, 822
PdiI GCCGGC 1 cut(s) 1009
PfeI GAWTC 2 cut(s) 688, 1780
PflFI GACNNNGTC 1 cut(s) 818
PinAI ACCGGT 1 cut(s) 945
PkrI GCNGC 2 cut(s) 386, 922
PleI GAGTC 1 cut(s) 1911
PpsI GAGTC 1 cut(s) 1911
Ppu21I YACGTR 1 cut(s) 1421
PshBI ATTAAT 1 cut(s) 1737
PsiI TTATAA 3 cut(s) 903, 1271, 1824
Psp124BI GAGCTC 1 cut(s) 699
PspN4I GGNNCC 1 cut(s) 1278
PspPI GGNCC 2 cut(s) 1020, 1277
PsuI RGATCY 1 cut(s) 1719
PsyI GACNNNGTC 1 cut(s) 818
PvuII CAGCTG 1 cut(s) 387
RsaI GTAC 7 cut(s) 616, 626, 1034, 1343, 1620, 1928, 1947
RsaNI GTAC 7 cut(s) 615, 625, 1033, 1342, 1619, 1927, 1946
SacI GAGCTC 1 cut(s) 699
SalI GTCGAC 1 cut(s) 821
SapI GCTCTTC 1 cut(s) 739
SatI GCNGC 2 cut(s) 385, 921
Sau3AI GATC 4 cut(s) 765, 1380, 1450, 1719
Sau96I GGNCC 2 cut(s) 1020, 1277
SchI GAGTC 1 cut(s) 1911
ScrFI CCNGG 1 cut(s) 809
SduI GDGCHC 2 cut(s) 699, 1472
SfaNI GCATC 4 cut(s) 664, 932, 1060, 1403
SfcI CTRYAG 1 cut(s) 484
SfuI TTCGAA 2 cut(s) 205, 449
SgrDI CGTCGACG 1 cut(s) 821
SmlI CTYRAG 1 cut(s) 731
SmoI CTYRAG 1 cut(s) 731
SseBI AGGCCT 1 cut(s) 662
SsiI CCGC 4 cut(s) 489, 517, 1228, 1723
SspI AATATT 3 cut(s) 326, 367, 1873
SspMI CTAG 2 cut(s) 1709, 1953
SstI GAGCTC 1 cut(s) 699
StuI AGGCCT 1 cut(s) 662
StyD4I CCNGG 1 cut(s) 807
StyI CCWWGG 6 cut(s) 429, 506, 663, 969, 1023, 1336
TaaI ACNGT 4 cut(s) 31, 314, 1244, 1816
TaiI ACGT 3 cut(s) 822, 981, 1423
TaqI TCGA 4 cut(s) 205, 449, 502, 822
TatI WGTACW 4 cut(s) 624, 1032, 1926, 1945
TfiI GAWTC 2 cut(s) 688, 1780
TscAI CASTG 2 cut(s) 1840, 1963
TseFI GTSAC 3 cut(s) 737, 974, 1440
TseI GCWGC 2 cut(s) 384, 920
Tsp45I GTSAC 3 cut(s) 737, 974, 1440
TspDTI ATGAA 8 cut(s) 53, 164, 563, 568, 1173, 1365, 1518, 1793
TspGWI ACGGA 1 cut(s) 756
TspRI CASTG 2 cut(s) 1840, 1963
Tth111I GACNNNGTC 1 cut(s) 818
VneI GTGCAC 1 cut(s) 1468
VspI ATTAAT 1 cut(s) 1737
XapI RAATTY 6 cut(s) 174, 352, 438, 461, 589, 1750
XcmI CCANNNNNNNNNTGG 2 cut(s) 1334, 1405
XmiI GTMKAC 2 cut(s) 822, 1770
XspI CTAG 2 cut(s) 1709, 1953
ZraI GACGTC 1 cut(s) 820
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.