pycom16g14340

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
N/A
Physical Location & Seq
Reverse (-)
10383201 .. 10383707
507 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 507 bp
ATGCGGCGAGATCCAGACGAAGACGACGAGGACAAATCCGAGGAGTACTCAGCCAACGATGAGGACGATGCCACCATGAGAAAGAGGGGGCTGGAAGATCCGGGCAACAGTCGGAGACCAGCTCATCCTTCAGCTCCGAGGCTGCTGCTGCTACAGATCTTGGTGTTTCTGGGTGGAGGCCAAGTTTGTAACAATTGCGATGGTGCCAATCATGACATTGAGAAGGAAGAAGACGACTTGCTTGCTGCCCTCCCAAGTCTTGAGGTTGTTGACTTGAGCTTCAACCGTCTCATCGGCAGCTTCCCACAACCCTCTTCAGAGCTGACTACTGTCGCCATCGACCTCATCGAAGATCGAGATCTTGAGCCCACCAACGAGGATCCCTCTCAACACCACCTGCAGAAATCTCTTCACCTGCACTTTCACAGGCCACTATTTCGGAGGCTAGAAGCTTGCCATAAATGTCACTCTATTCCGCTCTATTTTCACCATCGGCATCTGTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 ko05016 ko05168 ko05169 map00230 map00240 map01100 map03020 map05016 map05168 map05169
Pfam Domains
Protein Families
C2

Protein Analysis

169

Amino Acids

19.25

Weight (kDa)

5.09

Isoelectric Point (pI)

68.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 405, 423
Acc36I ACCTGC 2 cut(s) 405, 423
AccB1I GGYRCC 1 cut(s) 203
AccBSI CCGCTC 1 cut(s) 478
AciI CCGC 2 cut(s) 4, 476
AclWI GGATC 4 cut(s) 5, 92, 374, 387
AcuI CTGAAG 2 cut(s) 114, 300
AfaI GTAC 1 cut(s) 47
AgsI TTSAA 1 cut(s) 283
AluBI AGCT 6 cut(s) 122, 134, 279, 300, 322, 452
AluI AGCT 6 cut(s) 122, 134, 279, 300, 322, 452
Alw26I GTCTC 2 cut(s) 109, 293
AlwI GGATC 4 cut(s) 5, 92, 374, 387
AoxI GGCC 2 cut(s) 178, 428
ApeKI GCWGC 5 cut(s) 142, 145, 148, 245, 297
AsuC2I CCSGG 1 cut(s) 102
AsuHPI GGTGA 2 cut(s) 404, 479
BamHI GGATCC 1 cut(s) 379
BanI GGYRCC 1 cut(s) 203
BanII GRGCYC 1 cut(s) 369
BbsI GAAGAC 2 cut(s) 27, 237
BbvI GCAGC 5 cut(s) 129, 132, 135, 232, 309
BccI CCATC 3 cut(s) 194, 344, 498
BcgI CGANNNNNNTGC 4 cut(s) 127, 161, 224, 258
BcnI CCSGG 1 cut(s) 102
BcoDI GTCTC 2 cut(s) 109, 293
BfaI CTAG 2 cut(s) 446, 505
BfmI CTRYAG 2 cut(s) 152, 398
BfuAI ACCTGC 2 cut(s) 405, 423
BglII AGATCT 2 cut(s) 156, 358
BisI GCNGC 6 cut(s) 5, 143, 146, 149, 246, 298
BlsI GCNGC 6 cut(s) 6, 144, 147, 150, 247, 299
BmcAI AGTACT 1 cut(s) 47
Bme1390I CCNGG 1 cut(s) 102
BmiI GGNNCC 2 cut(s) 205, 381
BmrFI CCNGG 1 cut(s) 102
BmsI GCATC 1 cut(s) 58
BoxI GACNNNNGTC 1 cut(s) 329
BpiI GAAGAC 2 cut(s) 27, 237
BplI GAGNNNNNCTC 6 cut(s) 32, 64, 106, 138, 368, 400
BpuEI CTTGAG 3 cut(s) 281, 295, 383
BpuMI CCSGG 1 cut(s) 102
BsaBI GATNNNNATC 1 cut(s) 357
BsaI GGTCTC 1 cut(s) 109
BsaJI CCNNGG 2 cut(s) 39, 137
BsaXI ACNNNNNCTCC 2 cut(s) 168, 198
Bse8I GATNNNNATC 1 cut(s) 357
BseDI CCNNGG 2 cut(s) 39, 137
BseGI GGATG 1 cut(s) 124
BseJI GATNNNNATC 1 cut(s) 357
BseMII CTCAG 1 cut(s) 63
BseRI GAGGAG 1 cut(s) 56
BseXI GCAGC 5 cut(s) 129, 132, 135, 232, 309
BsgI GTGCAG 1 cut(s) 401
BshFI GGCC 2 cut(s) 180, 430
BshNI GGYRCC 1 cut(s) 203
BsiSI CCGG 1 cut(s) 101
BsmAI GTCTC 2 cut(s) 109, 293
BsmBI CGTCTC 1 cut(s) 293
BsnI GGCC 2 cut(s) 180, 430
Bso31I GGTCTC 1 cut(s) 109
Bsp1286I GDGCHC 1 cut(s) 369
Bsp143I GATC 6 cut(s) 10, 97, 156, 352, 358, 379
BspACI CCGC 2 cut(s) 4, 476
BspANI GGCC 2 cut(s) 180, 430
BspCNI CTCAG 1 cut(s) 62
BspHI TCATGA 1 cut(s) 211
BspLI GGNNCC 2 cut(s) 205, 381
BspMAI CTGCAG 1 cut(s) 402
BspMI ACCTGC 2 cut(s) 405, 423
BspPI GGATC 4 cut(s) 5, 92, 374, 387
BspT107I GGYRCC 1 cut(s) 203
BspTNI GGTCTC 1 cut(s) 109
BsrBI CCGCTC 1 cut(s) 478
BssECI CCNNGG 2 cut(s) 39, 137
BssMI GATC 6 cut(s) 10, 97, 156, 352, 358, 379
Bst4CI ACNGT 3 cut(s) 110, 287, 331
Bst6I CTCTTC 2 cut(s) 319, 414
BstC8I GCNNGC 2 cut(s) 243, 454
BstDEI CTNAG 1 cut(s) 49
BstF5I GGATG 1 cut(s) 124
BstKTI GATC 6 cut(s) 13, 100, 159, 355, 361, 382
BstMAI GTCTC 2 cut(s) 109, 293
BstMBI GATC 6 cut(s) 10, 97, 156, 352, 358, 379
BstMWI GCNNNNNNNGC 1 cut(s) 148
BstPAI GACNNNNGTC 1 cut(s) 329
BstSCI CCNGG 1 cut(s) 100
BstSFI CTRYAG 2 cut(s) 152, 398
BstV1I GCAGC 5 cut(s) 129, 132, 135, 232, 309
BstV2I GAAGAC 2 cut(s) 27, 237
BstX2I RGATCY 5 cut(s) 10, 97, 156, 358, 379
BstYI RGATCY 5 cut(s) 10, 97, 156, 358, 379
BsuRI GGCC 2 cut(s) 180, 430
BtgZI GCGATG 1 cut(s) 213
BtsCI GGATG 1 cut(s) 124
BveI ACCTGC 2 cut(s) 405, 423
Cac8I GCNNGC 2 cut(s) 243, 454
CciI TCATGA 1 cut(s) 211
Csp6I GTAC 1 cut(s) 46
CviAII CATG 2 cut(s) 76, 212
CviQI GTAC 1 cut(s) 46
DdeI CTNAG 1 cut(s) 49
DpnI GATC 6 cut(s) 12, 99, 158, 354, 360, 381
DpnII GATC 6 cut(s) 10, 97, 156, 352, 358, 379
Eam1104I CTCTTC 2 cut(s) 319, 414
EarI CTCTTC 2 cut(s) 319, 414
Eco24I GRGCYC 1 cut(s) 369
Eco31I GGTCTC 1 cut(s) 109
Eco57I CTGAAG 2 cut(s) 114, 300
EcoT38I GRGCYC 1 cut(s) 369
Esp3I CGTCTC 1 cut(s) 293
FaeI CATG 2 cut(s) 79, 215
FaiI YATR 3 cut(s) 77, 213, 459
FatI CATG 2 cut(s) 75, 211
Fnu4HI GCNGC 6 cut(s) 5, 143, 146, 149, 246, 298
FokI GGATG 1 cut(s) 111
FriOI GRGCYC 1 cut(s) 369
Fsp4HI GCNGC 6 cut(s) 5, 143, 146, 149, 246, 298
FspBI CTAG 2 cut(s) 446, 505
GluI GCNGC 6 cut(s) 5, 143, 146, 149, 246, 298
HaeIII GGCC 2 cut(s) 180, 430
HapII CCGG 1 cut(s) 101
Hin1II CATG 2 cut(s) 79, 215
HincII GTYRAC 1 cut(s) 271
HindII GTYRAC 1 cut(s) 271
HindIII AAGCTT 1 cut(s) 450
HpaII CCGG 1 cut(s) 101
HphI GGTGA 2 cut(s) 404, 479
Hpy166II GTNNAC 1 cut(s) 271
Hpy188I TCNGA 5 cut(s) 40, 114, 138, 319, 441
Hpy188III TCNNGA 5 cut(s) 14, 212, 260, 356, 362
Hpy8I GTNNAC 1 cut(s) 271
Hpy99I CGWCG 1 cut(s) 29
HpyAV CCTTC 2 cut(s) 138, 217
HpyCH4III ACNGT 3 cut(s) 110, 287, 331
HpyCH4V TGCA 2 cut(s) 400, 418
HpyF10VI GCNNNNNNNGC 1 cut(s) 148
HpyF3I CTNAG 1 cut(s) 49
Hsp92II CATG 2 cut(s) 79, 215
Kzo9I GATC 6 cut(s) 10, 97, 156, 352, 358, 379
LmnI GCTCC 1 cut(s) 139
LpnPI CCDG 8 cut(s) 27, 77, 114, 132, 155, 410, 412, 428
Lsp1109I GCAGC 5 cut(s) 129, 132, 135, 232, 309
LweI GCATC 1 cut(s) 58
MaeI CTAG 2 cut(s) 446, 505
MaeIII GTNAC 2 cut(s) 188, 464
MalI GATC 6 cut(s) 12, 99, 158, 354, 360, 381
MbiI CCGCTC 1 cut(s) 478
MboI GATC 6 cut(s) 10, 97, 156, 352, 358, 379
MboII GAAGA 7 cut(s) 32, 107, 239, 242, 306, 362, 401
MfeI CAATTG 1 cut(s) 193
MflI RGATCY 5 cut(s) 10, 97, 156, 358, 379
MhlI GDGCHC 1 cut(s) 369
MluCI AATT 1 cut(s) 193
MmeI TCCRAC 1 cut(s) 92
MspI CCGG 1 cut(s) 101
MspR9I CCNGG 1 cut(s) 102
MunI CAATTG 1 cut(s) 193
MwoI GCNNNNNNNGC 1 cut(s) 148
NciI CCSGG 1 cut(s) 102
NdeII GATC 6 cut(s) 10, 97, 156, 352, 358, 379
NlaIII CATG 2 cut(s) 79, 215
NlaIV GGNNCC 2 cut(s) 205, 381
NmuCI GTSAC 1 cut(s) 464
PagI TCATGA 1 cut(s) 211
PaqCI CACCTGC 2 cut(s) 405, 423
PcsI WCGNNNNNNNCGW 3 cut(s) 24, 63, 345
PkrI GCNGC 6 cut(s) 6, 144, 147, 150, 247, 299
PshAI GACNNNNGTC 1 cut(s) 329
PspN4I GGNNCC 2 cut(s) 205, 381
PstI CTGCAG 1 cut(s) 402
PsuI RGATCY 5 cut(s) 10, 97, 156, 358, 379
RsaI GTAC 1 cut(s) 47
RsaNI GTAC 1 cut(s) 46
SatI GCNGC 6 cut(s) 5, 143, 146, 149, 246, 298
Sau3AI GATC 6 cut(s) 10, 97, 156, 352, 358, 379
ScaI AGTACT 1 cut(s) 47
ScrFI CCNGG 1 cut(s) 102
SduI GDGCHC 1 cut(s) 369
SfaNI GCATC 1 cut(s) 58
SfcI CTRYAG 2 cut(s) 152, 398
SmlI CTYRAG 3 cut(s) 260, 274, 362
SmoI CTYRAG 3 cut(s) 260, 274, 362
Sse9I AATT 1 cut(s) 193
SsiI CCGC 2 cut(s) 4, 476
SspMI CTAG 2 cut(s) 446, 505
StyD4I CCNGG 1 cut(s) 100
TaaI ACNGT 3 cut(s) 110, 287, 331
TaqI TCGA 3 cut(s) 339, 348, 355
TasI AATT 1 cut(s) 193
TatI WGTACW 1 cut(s) 45
TauI GCSGC 1 cut(s) 7
TseFI GTSAC 1 cut(s) 464
TseI GCWGC 5 cut(s) 142, 145, 148, 245, 297
Tsp45I GTSAC 1 cut(s) 464
XspI CTAG 2 cut(s) 446, 505
ZrmI AGTACT 1 cut(s) 47
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.