pycom16g14910

LEC14B homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
N/A
Physical Location & Seq
Forward (+)
10923038 .. 10925085
2048 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 567 bp
ATGACGAAAGGAAAAGCAGCTGGGGTCCTGATGGGACATCTTGAAGGTGTTACATTTATTGATAGTCGGGGAGATGGGCATTACTTGATATCAAACGGGAAGGACCAGACTACCAAACTCTGGGATATAAGAAAGATGTCCTCTAGAGCCGTTTACAGCCCAAGGCGTACATATCGTGACTGGGATTACAGGTGGATGGAGTACCCAGCTCATGCAACAACTTTGAAACATCCCAATGATCAATCCTTGTCTACATATAGAGGCCATGCAGTCCTGCACACTTTAATTCGCTGTTACTTTTCTCCAGGATATAGTACCGGACAGAAGTACATCTACACTGGCTCAAGTGATCATTCTGTCTATATATATGATCTGGTGATTGGTGCTCAAGTTGCGAAACTAGACCATCATGAAGGACCTGTAAGGGACTCTAGTTGGCATCCTCTATATCCCATTTTGGTCAGCTCTTCTTGGGATGGGACCGTTGCCCGATGGGAATTTCCTGGTGATGACCAAGTTCCCACCCTGATGAGGCCAAAATTACGCCGGAGGAGAGTCTTCTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

189

Amino Acids

21.73

Weight (kDa)

9.47

Isoelectric Point (pI)

52.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EIF3I PF24805 3 - 161 8e-06 EIF3I
Beta-prop_WDR5 PF25175 79 - 165 2e-10 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 104 - 177 2e-09 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 104 - 168 4.4e-06 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 106 - 174 1.2e-07 WDHD1 first WD40 domain
WD40_Prp19 PF24814 108 - 172 3.3e-08 Prp19 WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 120
AccI GTMKAC 1 cut(s) 251
AcsI RAATTY 1 cut(s) 497
AfaI GTAC 4 cut(s) 169, 203, 316, 329
AfiI CCNNNNNNNGG 2 cut(s) 120, 531
AgsI TTSAA 2 cut(s) 44, 226
AjnI CCWGG 2 cut(s) 304, 502
AluBI AGCT 3 cut(s) 20, 209, 465
AluI AGCT 3 cut(s) 20, 209, 465
Alw21I GWGCWC 1 cut(s) 388
AoxI GGCC 2 cut(s) 262, 533
ApeKI GCWGC 1 cut(s) 17
ApoI RAATTY 1 cut(s) 497
AspS9I GGNCC 4 cut(s) 25, 103, 416, 480
AsuHPI GGTGA 2 cut(s) 388, 518
AvaII GGWCC 4 cut(s) 25, 103, 416, 480
BarI GAAGNNNNNNTAC 2 cut(s) 317, 349
BbsI GAAGAC 1 cut(s) 550
Bbv12I GWGCWC 1 cut(s) 388
BbvI GCAGC 1 cut(s) 29
BccI CCATC 6 cut(s) 25, 68, 190, 414, 470, 486
BceAI ACGGC 1 cut(s) 134
BciT130I CCWGG 2 cut(s) 306, 504
BclI TGATCA 2 cut(s) 238, 349
BfaI CTAG 3 cut(s) 144, 401, 432
BisI GCNGC 1 cut(s) 18
BlsI GCNGC 1 cut(s) 19
Bme1390I CCNGG 2 cut(s) 306, 504
Bme18I GGWCC 4 cut(s) 25, 103, 416, 480
BmgT120I GGNCC 4 cut(s) 25, 103, 416, 480
BmiI GGNNCC 2 cut(s) 26, 481
BmrFI CCNGG 2 cut(s) 306, 504
BmrI ACTGGG 1 cut(s) 190
BmsI GCATC 1 cut(s) 448
BmuI ACTGGG 1 cut(s) 190
BpiI GAAGAC 1 cut(s) 550
BpmI CTGGAG 1 cut(s) 288
BpuEI CTTGAG 2 cut(s) 328, 372
BsaJI CCNNGG 1 cut(s) 161
BsaWI WCCGGW 1 cut(s) 317
Bsc4I CCNNNNNNNGG 2 cut(s) 120, 531
Bse1I ACTGG 2 cut(s) 185, 343
BseBI CCWGG 2 cut(s) 306, 504
BseDI CCNNGG 1 cut(s) 161
BseGI GGATG 4 cut(s) 201, 229, 439, 481
BseLI CCNNNNNNNGG 2 cut(s) 120, 531
BseNI ACTGG 2 cut(s) 185, 343
BseRI GAGGAG 1 cut(s) 565
BseXI GCAGC 1 cut(s) 29
BseYI CCCAGC 2 cut(s) 20, 205
BsgI GTGCAG 1 cut(s) 260
BshFI GGCC 2 cut(s) 264, 535
BsiHKAI GWGCWC 1 cut(s) 388
BsiSI CCGG 2 cut(s) 318, 547
BslFI GGGAC 3 cut(s) 48, 440, 493
BslI CCNNNNNNNGG 2 cut(s) 120, 531
BsmFI GGGAC 3 cut(s) 48, 440, 493
BsnI GGCC 2 cut(s) 264, 535
Bsp1286I GDGCHC 1 cut(s) 388
Bsp143I GATC 3 cut(s) 238, 349, 370
BspANI GGCC 2 cut(s) 264, 535
BspHI TCATGA 1 cut(s) 409
BspLI GGNNCC 2 cut(s) 26, 481
BspQI GCTCTTC 1 cut(s) 472
BsrI ACTGG 2 cut(s) 185, 343
BssECI CCNNGG 1 cut(s) 161
BssMI GATC 3 cut(s) 238, 349, 370
BssT1I CCWWGG 1 cut(s) 161
Bst2UI CCWGG 2 cut(s) 306, 504
Bst4CI ACNGT 1 cut(s) 484
Bst6I CTCTTC 1 cut(s) 472
BstF5I GGATG 4 cut(s) 201, 229, 439, 481
BstKTI GATC 3 cut(s) 241, 352, 373
BstMBI GATC 3 cut(s) 238, 349, 370
BstMWI GCNNNNNNNGC 1 cut(s) 392
BstNI CCWGG 2 cut(s) 306, 504
BstSCI CCNGG 2 cut(s) 304, 502
BstV1I GCAGC 1 cut(s) 29
BstV2I GAAGAC 1 cut(s) 550
BsuRI GGCC 2 cut(s) 264, 535
BtsCI GGATG 4 cut(s) 201, 229, 439, 481
BtsIMutI CAGTG 1 cut(s) 336
CciI TCATGA 1 cut(s) 409
Cfr13I GGNCC 4 cut(s) 25, 103, 416, 480
Csp6I GTAC 4 cut(s) 168, 202, 315, 328
CviAII CATG 3 cut(s) 212, 266, 410
CviJI RGCY 8 cut(s) 20, 149, 159, 209, 264, 342, 465, 535
CviKI_1 RGCY 8 cut(s) 20, 149, 159, 209, 264, 342, 465, 535
CviQI GTAC 4 cut(s) 168, 202, 315, 328
DpnI GATC 3 cut(s) 240, 351, 372
DpnII GATC 3 cut(s) 238, 349, 370
Eam1104I CTCTTC 1 cut(s) 472
EarI CTCTTC 1 cut(s) 472
Eco130I CCWWGG 1 cut(s) 161
Eco32I GATATC 1 cut(s) 90
Eco47I GGWCC 4 cut(s) 25, 103, 416, 480
EcoO109I RGGNCCY 2 cut(s) 25, 416
EcoRII CCWGG 2 cut(s) 304, 502
EcoRV GATATC 1 cut(s) 90
EcoT14I CCWWGG 1 cut(s) 161
ErhI CCWWGG 1 cut(s) 161
FaeI CATG 3 cut(s) 215, 269, 413
FaqI GGGAC 3 cut(s) 48, 440, 493
FatI CATG 3 cut(s) 211, 265, 409
FbaI TGATCA 2 cut(s) 238, 349
FblI GTMKAC 1 cut(s) 251
Fnu4HI GCNGC 1 cut(s) 18
FokI GGATG 4 cut(s) 208, 216, 426, 488
Fsp4HI GCNGC 1 cut(s) 18
FspBI CTAG 3 cut(s) 144, 401, 432
GluI GCNGC 1 cut(s) 18
GsaI CCCAGC 2 cut(s) 24, 209
GsuI CTGGAG 1 cut(s) 288
HaeIII GGCC 2 cut(s) 264, 535
HapII CCGG 2 cut(s) 318, 547
Hin1II CATG 3 cut(s) 215, 269, 413
HinfI GANTC 2 cut(s) 428, 555
HpaII CCGG 2 cut(s) 318, 547
HphI GGTGA 2 cut(s) 388, 518
Hpy166II GTNNAC 2 cut(s) 154, 252
Hpy188III TCNNGA 5 cut(s) 28, 41, 144, 176, 410
Hpy8I GTNNAC 2 cut(s) 154, 252
HpyAV CCTTC 3 cut(s) 38, 94, 407
HpyCH4III ACNGT 1 cut(s) 484
HpyCH4V TGCA 3 cut(s) 215, 269, 277
HpyF10VI GCNNNNNNNGC 1 cut(s) 392
Hsp92II CATG 3 cut(s) 215, 269, 413
Ksp22I TGATCA 2 cut(s) 238, 349
Kzo9I GATC 3 cut(s) 238, 349, 370
LguI GCTCTTC 1 cut(s) 472
Lsp1109I GCAGC 1 cut(s) 29
LweI GCATC 1 cut(s) 448
MaeI CTAG 3 cut(s) 144, 401, 432
MaeIII GTNAC 3 cut(s) 49, 176, 293
MalI GATC 3 cut(s) 240, 351, 372
MboI GATC 3 cut(s) 238, 349, 370
MboII GAAGA 2 cut(s) 459, 550
MhlI GDGCHC 1 cut(s) 388
MluCI AATT 3 cut(s) 285, 497, 539
MlyI GAGTC 2 cut(s) 422, 564
MnlI CCTC 5 cut(s) 151, 254, 453, 525, 543
MseI TTAA 1 cut(s) 284
MslI CAYNNNNRTG 2 cut(s) 234, 527
MspA1I CMGCKG 1 cut(s) 20
MspI CCGG 2 cut(s) 318, 547
MspR9I CCNGG 2 cut(s) 306, 504
MvaI CCWGG 2 cut(s) 306, 504
MwoI GCNNNNNNNGC 1 cut(s) 392
NdeII GATC 3 cut(s) 238, 349, 370
NlaIII CATG 3 cut(s) 215, 269, 413
NlaIV GGNNCC 2 cut(s) 26, 481
NmuCI GTSAC 1 cut(s) 176
PagI TCATGA 1 cut(s) 409
PciSI GCTCTTC 1 cut(s) 472
PflMI CCANNNNNTGG 1 cut(s) 120
PfoI TCCNGGA 1 cut(s) 304
PkrI GCNGC 1 cut(s) 19
PleI GAGTC 2 cut(s) 422, 563
PpsI GAGTC 2 cut(s) 422, 563
PpuMI RGGWCCY 2 cut(s) 25, 416
Psp5II RGGWCCY 2 cut(s) 25, 416
Psp6I CCWGG 2 cut(s) 304, 502
PspFI CCCAGC 2 cut(s) 20, 205
PspGI CCWGG 2 cut(s) 304, 502
PspN4I GGNNCC 2 cut(s) 26, 481
PspPI GGNCC 4 cut(s) 25, 103, 416, 480
PspPPI RGGWCCY 2 cut(s) 25, 416
PvuII CAGCTG 1 cut(s) 20
RsaI GTAC 4 cut(s) 169, 203, 316, 329
RsaNI GTAC 4 cut(s) 168, 202, 315, 328
RseI CAYNNNNRTG 2 cut(s) 234, 527
SapI GCTCTTC 1 cut(s) 472
SaqAI TTAA 1 cut(s) 284
SatI GCNGC 1 cut(s) 18
Sau3AI GATC 3 cut(s) 238, 349, 370
Sau96I GGNCC 4 cut(s) 25, 103, 416, 480
SchI GAGTC 2 cut(s) 422, 564
ScrFI CCNGG 2 cut(s) 306, 504
SduI GDGCHC 1 cut(s) 388
SetI ASST 6 cut(s) 22, 49, 194, 211, 421, 467
SfaNI GCATC 1 cut(s) 448
SinI GGWCC 4 cut(s) 25, 103, 416, 480
SmiMI CAYNNNNRTG 2 cut(s) 234, 527
SmlI CTYRAG 2 cut(s) 343, 387
SmoI CTYRAG 2 cut(s) 343, 387
Sse9I AATT 3 cut(s) 285, 497, 539
SspMI CTAG 3 cut(s) 144, 401, 432
StyD4I CCNGG 2 cut(s) 304, 502
StyI CCWWGG 1 cut(s) 161
TaaI ACNGT 1 cut(s) 484
TasI AATT 3 cut(s) 285, 497, 539
TatI WGTACW 1 cut(s) 327
Tru1I TTAA 1 cut(s) 284
Tru9I TTAA 1 cut(s) 284
TscAI CASTG 1 cut(s) 343
TseFI GTSAC 1 cut(s) 176
TseI GCWGC 1 cut(s) 17
Tsp45I GTSAC 1 cut(s) 176
TspDTI ATGAA 1 cut(s) 426
TspRI CASTG 1 cut(s) 343
Van91I CCANNNNNTGG 1 cut(s) 120
VpaK11BI GGWCC 4 cut(s) 25, 103, 416, 480
XapI RAATTY 1 cut(s) 497
XbaI TCTAGA 1 cut(s) 143
XmiI GTMKAC 1 cut(s) 251
XspI CTAG 3 cut(s) 144, 401, 432
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.