pycom16g17100

E3 ubiquitin-protein ligase ORTHRUS

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
N/A
Physical Location & Seq
Forward (+)
13159322 .. 13159855
534 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 441 bp
ATGGAAGCCGGAGTGGAACAAACTCCCGGTGATGGAGCAGGCGAGGTTGAAAGCGAGGCAAGGAGTGATGACGATGGCAGAGGCAAATTCATCGGGGAGATAGTTTTGAGAAGGGGGGTTAGCCAGCTTCCCTACGACGGAGATGGCGTCTGCATGCTTTGCAAGGTGACTATGCCCGACACCAAGAAGCTTACCTACAAGACCTCCGCCACTCTTCAGTGGAAGTGCCCCGACTGCAGATCAATCTCCAGCAACGCTTATACGCATTTACCCCCCATGAACGTTGTCGTGGACGGTGGTCTAGTGTTGGCGATTCGGGAGATCGAGAACAACGTGACCCTGACAGAGCAGGAAAAGGCAAGGAGGAGGCAGGAGGTGGGTGGGCGCAGAGGAAATAAGAGAAAAGGGAAGGATGGCTGGGTAGGAGCAAAGGATGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0000228 GO:0000785 GO:0000790 GO:0000792 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005720 GO:0005737 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006323 GO:0006325 GO:0006333 GO:0006338 GO:0006355 GO:0006464 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0008327 GO:0009889 GO:0009893 GO:0009987 GO:0010216 GO:0010369 GO:0010385 GO:0010424 GO:0010428 GO:0010429 GO:0010468 GO:0010556 GO:0010604 GO:0010638 GO:0016043 GO:0016567 GO:0016740 GO:0019219 GO:0019222 GO:0019538 GO:0019787 GO:0022607 GO:0031055 GO:0031323 GO:0031325 GO:0031326 GO:0031497 GO:0031507 GO:0031508 GO:0031935 GO:0031937 GO:0031974 GO:0031981 GO:0032259 GO:0032446 GO:0032776 GO:0033043 GO:0033044 GO:0034508 GO:0034622 GO:0034641 GO:0036094 GO:0036211 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043565 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044728 GO:0045935 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051252 GO:0051276 GO:0051301 GO:0060255 GO:0060968 GO:0061630 GO:0061659 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0070647 GO:0070828 GO:0071103 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0090308 GO:0090309 GO:0097159 GO:0098687 GO:0140096 GO:1901265 GO:1901360 GO:1901363 GO:1901564 GO:1902275 GO:1903506 GO:1905269 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAD_SRA zf-C3HC4 zf-C3HC4_3 zf-RING_UBOX

Protein Analysis

147

Amino Acids

15.91

Weight (kDa)

7.63

Isoelectric Point (pI)

57.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 207
AclI AACGTT 1 cut(s) 282
AcsI RAATTY 1 cut(s) 86
AcuI CTGAAG 1 cut(s) 200
AcyI GRCGYC 1 cut(s) 147
AfiI CCNNNNNNNGG 2 cut(s) 32, 137
AgsI TTSAA 1 cut(s) 50
AluBI AGCT 2 cut(s) 127, 190
AluI AGCT 2 cut(s) 127, 190
ApoI RAATTY 1 cut(s) 86
AspLEI GCGC 1 cut(s) 387
AsuC2I CCSGG 1 cut(s) 27
AsuHPI GGTGA 2 cut(s) 41, 178
BaeGI GKGCMC 1 cut(s) 230
BarI GAAGNNNNNNTAC 2 cut(s) 179, 211
BccI CCATC 5 cut(s) 26, 68, 137, 407, 428
BcgI CGANNNNNNTGC 2 cut(s) 73, 107
BcnI CCSGG 1 cut(s) 27
BfaI CTAG 1 cut(s) 302
BfmI CTRYAG 1 cut(s) 235
Bme1390I CCNGG 1 cut(s) 27
BmrFI CCNGG 1 cut(s) 27
BoxI GACNNNNGTC 1 cut(s) 297
BpmI CTGGAG 1 cut(s) 232
BpuMI CCSGG 1 cut(s) 27
BsaHI GRCGYC 1 cut(s) 147
BsaXI ACNNNNNCTCC 4 cut(s) 132, 162, 188, 218
Bsc4I CCNNNNNNNGG 2 cut(s) 32, 137
BseGI GGATG 2 cut(s) 418, 439
BseLI CCNNNNNNNGG 2 cut(s) 32, 137
BseRI GAGGAG 1 cut(s) 379
BseSI GKGCMC 1 cut(s) 230
BseYI CCCAGC 1 cut(s) 417
BsiSI CCGG 2 cut(s) 9, 27
BslI CCNNNNNNNGG 2 cut(s) 32, 137
Bsp1286I GDGCHC 1 cut(s) 230
Bsp143I GATC 2 cut(s) 239, 321
BspACI CCGC 1 cut(s) 207
BspMAI CTGCAG 1 cut(s) 239
BssMI GATC 2 cut(s) 239, 321
BssNI GRCGYC 1 cut(s) 147
Bst4CI ACNGT 1 cut(s) 296
Bst6I CTCTTC 1 cut(s) 219
BstACI GRCGYC 1 cut(s) 147
BstAPI GCANNNNNTGC 1 cut(s) 159
BstC8I GCNNGC 3 cut(s) 40, 125, 155
BstF5I GGATG 2 cut(s) 418, 439
BstHHI GCGC 1 cut(s) 387
BstKTI GATC 2 cut(s) 242, 324
BstMBI GATC 2 cut(s) 239, 321
BstMWI GCNNNNNNNGC 2 cut(s) 159, 234
BstNSI RCATGY 1 cut(s) 157
BstPAI GACNNNNGTC 1 cut(s) 297
BstSCI CCNGG 1 cut(s) 25
BstSFI CTRYAG 1 cut(s) 235
BstSLI GKGCMC 1 cut(s) 230
BtsCI GGATG 2 cut(s) 418, 439
BtsIMutI CAGTG 1 cut(s) 224
Cac8I GCNNGC 3 cut(s) 40, 125, 155
CfoI GCGC 1 cut(s) 387
CseI GACGC 1 cut(s) 136
CviAII CATG 2 cut(s) 154, 277
CviJI RGCY 5 cut(s) 8, 123, 127, 190, 417
CviKI_1 RGCY 5 cut(s) 8, 123, 127, 190, 417
DpnI GATC 2 cut(s) 241, 323
DpnII GATC 2 cut(s) 239, 321
Eam1104I CTCTTC 1 cut(s) 219
EarI CTCTTC 1 cut(s) 219
EciI GGCGGA 1 cut(s) 196
Eco57I CTGAAG 1 cut(s) 200
FaeI CATG 2 cut(s) 157, 280
FaiI YATR 4 cut(s) 155, 173, 261, 278
FatI CATG 2 cut(s) 153, 276
FokI GGATG 1 cut(s) 425
FspBI CTAG 1 cut(s) 302
GlaI GCGC 1 cut(s) 386
GsaI CCCAGC 1 cut(s) 421
GsuI CTGGAG 1 cut(s) 232
HapII CCGG 2 cut(s) 9, 27
HgaI GACGC 1 cut(s) 136
HhaI GCGC 1 cut(s) 387
Hin1I GRCGYC 1 cut(s) 147
Hin1II CATG 2 cut(s) 157, 280
Hin6I GCGC 1 cut(s) 385
HinP1I GCGC 1 cut(s) 385
HindIII AAGCTT 1 cut(s) 188
HinfI GANTC 1 cut(s) 313
HpaII CCGG 2 cut(s) 9, 27
HphI GGTGA 2 cut(s) 41, 178
Hpy166II GTNNAC 1 cut(s) 292
Hpy188III TCNNGA 2 cut(s) 317, 325
Hpy8I GTNNAC 1 cut(s) 292
Hpy99I CGWCG 1 cut(s) 140
HpyAV CCTTC 2 cut(s) 105, 403
HpyCH4III ACNGT 1 cut(s) 296
HpyCH4IV ACGT 2 cut(s) 282, 333
HpyCH4V TGCA 3 cut(s) 153, 162, 237
HpyF10VI GCNNNNNNNGC 2 cut(s) 159, 234
HpySE526I ACGT 2 cut(s) 282, 333
Hsp92I GRCGYC 1 cut(s) 147
Hsp92II CATG 2 cut(s) 157, 280
HspAI GCGC 1 cut(s) 385
Kzo9I GATC 2 cut(s) 239, 321
LmnI GCTCC 2 cut(s) 35, 425
LpnPI CCDG 9 cut(s) 22, 24, 40, 137, 262, 335, 353, 356, 403
MaeI CTAG 1 cut(s) 302
MaeII ACGT 2 cut(s) 282, 333
MaeIII GTNAC 2 cut(s) 166, 334
MalI GATC 2 cut(s) 241, 323
MboI GATC 2 cut(s) 239, 321
MboII GAAGA 1 cut(s) 206
MhlI GDGCHC 1 cut(s) 230
MluCI AATT 1 cut(s) 86
MnlI CCTC 8 cut(s) 37, 49, 74, 214, 357, 360, 367, 383
MspI CCGG 2 cut(s) 9, 27
MspR9I CCNGG 1 cut(s) 27
MwoI GCNNNNNNNGC 2 cut(s) 159, 234
NciI CCSGG 1 cut(s) 27
NdeII GATC 2 cut(s) 239, 321
NlaIII CATG 2 cut(s) 157, 280
NmuCI GTSAC 2 cut(s) 166, 334
NspI RCATGY 1 cut(s) 157
PaeI GCATGC 1 cut(s) 157
PcsI WCGNNNNNNNCGW 2 cut(s) 144, 330
PfeI GAWTC 1 cut(s) 313
PshAI GACNNNNGTC 1 cut(s) 297
Psp1406I AACGTT 1 cut(s) 282
PspFI CCCAGC 1 cut(s) 417
PstI CTGCAG 1 cut(s) 239
Sau3AI GATC 2 cut(s) 239, 321
ScrFI CCNGG 1 cut(s) 27
SduI GDGCHC 1 cut(s) 230
SetI ASST 9 cut(s) 48, 129, 168, 192, 197, 206, 285, 336, 378
SfcI CTRYAG 1 cut(s) 235
SphI GCATGC 1 cut(s) 157
Sse9I AATT 1 cut(s) 86
SsiI CCGC 1 cut(s) 207
SspMI CTAG 1 cut(s) 302
StyD4I CCNGG 1 cut(s) 25
TaaI ACNGT 1 cut(s) 296
TaiI ACGT 2 cut(s) 285, 336
TaqI TCGA 1 cut(s) 324
TasI AATT 1 cut(s) 86
TfiI GAWTC 1 cut(s) 313
TscAI CASTG 1 cut(s) 224
TseFI GTSAC 2 cut(s) 166, 334
Tsp45I GTSAC 2 cut(s) 166, 334
TspDTI ATGAA 2 cut(s) 79, 293
TspGWI ACGGA 1 cut(s) 153
TspRI CASTG 1 cut(s) 224
XapI RAATTY 1 cut(s) 86
XceI RCATGY 1 cut(s) 157
XspI CTAG 1 cut(s) 302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.