pycom16g18050

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
N/A
Physical Location & Seq
Forward (+)
14469646 .. 14470147
502 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 396 bp
ATGGGTCTGCAACTGATCTGGAGTTTAGTACTTGCACTGCTAGATGTATACTCCTTGGTGAAAATGAAGGTCCTCCACAACCCTGTATTGGTTAGCCTCTTTGTTGTTGGAGATTGGGTTACAGCAACATTGTCTCTTGCAGCAGCTTCGGCCTCAGCAGGCATAACCGTGTTGTACTTTAGTGATCTCCGGAGAGGAATGCCAGAAATACCAAATTGCAGTTGGTTTGGCGTACATGAGCTGGCTAACAATTGCAATTTCTTCTCTGATTATGCTCGGGCTATTAGCAGCATGTTAGATAGTCCTCCTCTGTCATACATATTTCTCTTTCTCTTTACAACAAAAACAAGAAATTGCAGTGTCTATAGAAAAGGAAACAAACTTGATAGCGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

132

Amino Acids

14.52

Weight (kDa)

8.5

Isoelectric Point (pI)

33.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 1 - 60 8.1e-13 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 48
AccIII TCCGGA 1 cut(s) 189
AfaI GTAC 3 cut(s) 30, 176, 234
AfiI CCNNNNNNNGG 1 cut(s) 88
AluBI AGCT 2 cut(s) 146, 241
AluI AGCT 2 cut(s) 146, 241
Alw26I GTCTC 1 cut(s) 138
Ama87I CYCGRG 1 cut(s) 276
Aor13HI TCCGGA 1 cut(s) 189
AoxI GGCC 1 cut(s) 150
ApeKI GCWGC 3 cut(s) 140, 143, 288
AspLEI GCGC 1 cut(s) 392
AspS9I GGNCC 1 cut(s) 70
AsuHPI GGTGA 1 cut(s) 70
AvaI CYCGRG 1 cut(s) 276
AvaII GGWCC 1 cut(s) 70
BbvCI CCTCAGC 1 cut(s) 154
BbvI GCAGC 3 cut(s) 152, 155, 300
BcgI CGANNNNNNTGC 2 cut(s) 129, 163
BcoDI GTCTC 1 cut(s) 138
BfaI CTAG 1 cut(s) 41
BfmI CTRYAG 1 cut(s) 364
BisI GCNGC 3 cut(s) 141, 144, 289
BlsI GCNGC 3 cut(s) 142, 145, 290
BmcAI AGTACT 1 cut(s) 30
Bme18I GGWCC 1 cut(s) 70
BmeT110I CYCGRG 1 cut(s) 276
BmgT120I GGNCC 1 cut(s) 70
BpmI CTGGAG 1 cut(s) 40
Bpu10I CCTNAGC 1 cut(s) 154
BsaJI CCNNGG 1 cut(s) 54
BsaWI WCCGGW 1 cut(s) 189
BsaXI ACNNNNNCTCC 2 cut(s) 102, 132
Bsc4I CCNNNNNNNGG 1 cut(s) 88
BseAI TCCGGA 1 cut(s) 189
BseDI CCNNGG 1 cut(s) 54
BseLI CCNNNNNNNGG 1 cut(s) 88
BseMII CTCAG 1 cut(s) 168
BseRI GAGGAG 1 cut(s) 297
BseXI GCAGC 3 cut(s) 152, 155, 300
BshFI GGCC 1 cut(s) 152
BsiHKCI CYCGRG 1 cut(s) 276
BsiSI CCGG 1 cut(s) 190
BslI CCNNNNNNNGG 1 cut(s) 88
BsmAI GTCTC 1 cut(s) 138
BsmI GAATGC 1 cut(s) 204
BsnI GGCC 1 cut(s) 152
BsoBI CYCGRG 1 cut(s) 276
Bsp13I TCCGGA 1 cut(s) 189
Bsp143I GATC 2 cut(s) 15, 184
BspANI GGCC 1 cut(s) 152
BspCNI CTCAG 1 cut(s) 167
BspEI TCCGGA 1 cut(s) 189
BssECI CCNNGG 1 cut(s) 54
BssMI GATC 2 cut(s) 15, 184
BssNAI GTATAC 1 cut(s) 49
BssT1I CCWWGG 1 cut(s) 54
Bst1107I GTATAC 1 cut(s) 49
Bst4CI ACNGT 1 cut(s) 169
BstC8I GCNNGC 2 cut(s) 160, 243
BstDEI CTNAG 1 cut(s) 154
BstHHI GCGC 1 cut(s) 392
BstKTI GATC 2 cut(s) 18, 187
BstMAI GTCTC 1 cut(s) 138
BstMBI GATC 2 cut(s) 15, 184
BstMWI GCNNNNNNNGC 1 cut(s) 149
BstNSI RCATGY 1 cut(s) 295
BstSFI CTRYAG 1 cut(s) 364
BstV1I GCAGC 3 cut(s) 152, 155, 300
BstZ17I GTATAC 1 cut(s) 49
BsuRI GGCC 1 cut(s) 152
BtsI GCAGTG 2 cut(s) 35, 364
BtsIMutI CAGTG 2 cut(s) 35, 364
Cac8I GCNNGC 2 cut(s) 160, 243
CfoI GCGC 1 cut(s) 392
Cfr13I GGNCC 1 cut(s) 70
Csp6I GTAC 3 cut(s) 29, 175, 233
CviAII CATG 2 cut(s) 236, 292
CviJI RGCY 6 cut(s) 96, 146, 152, 241, 245, 281
CviKI_1 RGCY 6 cut(s) 96, 146, 152, 241, 245, 281
CviQI GTAC 3 cut(s) 29, 175, 233
DdeI CTNAG 1 cut(s) 154
DpnI GATC 2 cut(s) 17, 186
DpnII GATC 2 cut(s) 15, 184
Eco130I CCWWGG 1 cut(s) 54
Eco47I GGWCC 1 cut(s) 70
Eco88I CYCGRG 1 cut(s) 276
EcoO109I RGGNCCY 1 cut(s) 70
EcoT14I CCWWGG 1 cut(s) 54
ErhI CCWWGG 1 cut(s) 54
FaeI CATG 2 cut(s) 239, 295
FaiI YATR 9 cut(s) 49, 164, 237, 273, 293, 316, 320, 366, 394
FatI CATG 2 cut(s) 235, 291
FblI GTMKAC 1 cut(s) 48
Fnu4HI GCNGC 3 cut(s) 141, 144, 289
Fsp4HI GCNGC 3 cut(s) 141, 144, 289
FspBI CTAG 1 cut(s) 41
GlaI GCGC 1 cut(s) 391
GluI GCNGC 3 cut(s) 141, 144, 289
GsuI CTGGAG 1 cut(s) 40
HaeIII GGCC 1 cut(s) 152
HapII CCGG 1 cut(s) 190
HhaI GCGC 1 cut(s) 392
Hin1II CATG 2 cut(s) 239, 295
Hin6I GCGC 1 cut(s) 390
HinP1I GCGC 1 cut(s) 390
HpaII CCGG 1 cut(s) 190
HphI GGTGA 1 cut(s) 70
Hpy166II GTNNAC 1 cut(s) 49
Hpy188I TCNGA 1 cut(s) 268
Hpy188III TCNNGA 2 cut(s) 19, 190
Hpy8I GTNNAC 1 cut(s) 49
HpyAV CCTTC 1 cut(s) 61
HpyCH4III ACNGT 1 cut(s) 169
HpyCH4V TGCA 6 cut(s) 10, 35, 140, 219, 255, 357
HpyF10VI GCNNNNNNNGC 1 cut(s) 149
HpyF3I CTNAG 1 cut(s) 154
Hsp92II CATG 2 cut(s) 239, 295
HspAI GCGC 1 cut(s) 390
Kpn2I TCCGGA 1 cut(s) 189
Kzo9I GATC 2 cut(s) 15, 184
LpnPI CCDG 6 cut(s) 4, 96, 144, 203, 216, 227
Lsp1109I GCAGC 3 cut(s) 152, 155, 300
MaeI CTAG 1 cut(s) 41
MaeIII GTNAC 1 cut(s) 118
MalI GATC 2 cut(s) 17, 186
MboI GATC 2 cut(s) 15, 184
MboII GAAGA 1 cut(s) 253
MfeI CAATTG 1 cut(s) 250
MluCI AATT 4 cut(s) 214, 250, 256, 352
MmeI TCCRAC 1 cut(s) 88
MnlI CCTC 6 cut(s) 83, 107, 163, 188, 315, 318
MroI TCCGGA 1 cut(s) 189
MslI CAYNNNNRTG 1 cut(s) 167
MspI CCGG 1 cut(s) 190
MunI CAATTG 1 cut(s) 250
Mva1269I GAATGC 1 cut(s) 204
MwoI GCNNNNNNNGC 1 cut(s) 149
NdeII GATC 2 cut(s) 15, 184
NlaIII CATG 2 cut(s) 239, 295
NspI RCATGY 1 cut(s) 295
PctI GAATGC 1 cut(s) 204
PkrI GCNGC 3 cut(s) 142, 145, 290
PpuMI RGGWCCY 1 cut(s) 70
Psp5II RGGWCCY 1 cut(s) 70
PspPI GGNCC 1 cut(s) 70
PspPPI RGGWCCY 1 cut(s) 70
RsaI GTAC 3 cut(s) 30, 176, 234
RsaNI GTAC 3 cut(s) 29, 175, 233
RseI CAYNNNNRTG 1 cut(s) 167
SatI GCNGC 3 cut(s) 141, 144, 289
Sau3AI GATC 2 cut(s) 15, 184
Sau96I GGNCC 1 cut(s) 70
ScaI AGTACT 1 cut(s) 30
SetI ASST 3 cut(s) 72, 148, 243
SfcI CTRYAG 1 cut(s) 364
SinI GGWCC 1 cut(s) 70
SmiMI CAYNNNNRTG 1 cut(s) 167
Sse9I AATT 4 cut(s) 214, 250, 256, 352
SspMI CTAG 1 cut(s) 41
StyI CCWWGG 1 cut(s) 54
TaaI ACNGT 1 cut(s) 169
TasI AATT 4 cut(s) 214, 250, 256, 352
TatI WGTACW 2 cut(s) 28, 174
TscAI CASTG 2 cut(s) 42, 364
TseI GCWGC 3 cut(s) 140, 143, 288
TspDTI ATGAA 1 cut(s) 80
TspRI CASTG 2 cut(s) 42, 364
VpaK11BI GGWCC 1 cut(s) 70
XceI RCATGY 1 cut(s) 295
XcmI CCANNNNNNNNNTGG 1 cut(s) 219
XmiI GTMKAC 1 cut(s) 48
XspI CTAG 1 cut(s) 41
ZrmI AGTACT 1 cut(s) 30
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.