pycom16g18190

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
N/A
Physical Location & Seq
Forward (+)
14751145 .. 14751991
847 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 705 bp
ATGACTAGACAGAAGGTACAATTCATTGGTAATCAACTAGGAGAGTACGTTGTGGCAGATCAGAGTAGGAAGGGTAAAGCGATGGGAAGTATCCTACGAAATAATGCGAACTATTCCGAGGATGTAACGATGATGACTTATCTAAGCCATACGGTCGATGGTTTCAAAAAGATGTCCTTCATGCACAGGGAGTATCGTAAACCGTTGAGGAAACGTTTTGCTTTGGATCCCTCCCCAGCTTGGTCTATGAAAGCTCCGTTGCATGAAGAAGAGGAAGAACCACCGGAGCAAGCTGGGAGACGTCGGAGTGGTGGAGTTTCGGAGGTTGATCTGAATGTTGAATATGGAAAACTTGGTAATTATGGCGAGTACGAATTTGGTGGGGAAACCAGAGCCATTAGGGCAAATTTACTTGATTTGAATGACGTAGCGGATTCTAGGGATGAGGCAGTTGACTCTTTAGCCTTAATTCCTTTTCAAGATTCAGGGCTGTTGATTGCCCTGTTGTCAATTTTAATGATCACAGTCAGCGTAGCAACATGTAAAATAGAATTCAAACTAACCTCCAGCCATGATCGTCCAACAACAGGTTTCCAGCAGAACAACAAATTCGAAAGCTCATGTAACTCTAGTTTAAGTGATCTTCTAGCCTATTGCCACTCAAGTGCCTTTCTCAGATGGAATGCAAGGCATTATATAGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

235

Amino Acids

26.36

Weight (kDa)

6.32

Isoelectric Point (pI)

57.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 304
AciI CCGC 1 cut(s) 431
AclI AACGTT 1 cut(s) 214
AclWI GGATC 2 cut(s) 221, 234
AcsI RAATTY 4 cut(s) 374, 406, 551, 608
AcyI GRCGYC 1 cut(s) 301
AfaI GTAC 3 cut(s) 18, 47, 371
AfiI CCNNNNNNNGG 2 cut(s) 240, 587
AflIII ACRYGT 1 cut(s) 539
AgsI TTSAA 5 cut(s) 166, 341, 421, 479, 556
AleI CACNNNNGTG 1 cut(s) 663
AluBI AGCT 4 cut(s) 239, 254, 293, 618
AluI AGCT 4 cut(s) 239, 254, 293, 618
Alw26I GTCTC 1 cut(s) 292
AlwI GGATC 2 cut(s) 221, 234
ApoI RAATTY 4 cut(s) 374, 406, 551, 608
AsuII TTCGAA 1 cut(s) 612
BamHI GGATCC 1 cut(s) 226
BccI CCATC 3 cut(s) 76, 152, 672
BciVI GTATCC 1 cut(s) 101
BclI TGATCA 1 cut(s) 519
BcoDI GTCTC 1 cut(s) 292
BfaI CTAG 6 cut(s) 6, 38, 438, 630, 647, 703
BfuI GTATCC 1 cut(s) 101
BglI GCCNNNNNGGC 1 cut(s) 401
BmiI GGNNCC 1 cut(s) 228
BpmI CTGGAG 1 cut(s) 550
Bpu14I TTCGAA 1 cut(s) 612
BpuEI CTTGAG 1 cut(s) 646
BsaHI GRCGYC 1 cut(s) 301
BsaJI CCNNGG 1 cut(s) 117
BsaWI WCCGGW 1 cut(s) 283
BsaXI ACNNNNNCTCC 2 cut(s) 33, 63
Bsc4I CCNNNNNNNGG 2 cut(s) 240, 587
BseDI CCNNGG 1 cut(s) 117
BseGI GGATG 2 cut(s) 127, 448
BseLI CCNNNNNNNGG 2 cut(s) 240, 587
BseMII CTCAG 1 cut(s) 688
BseYI CCCAGC 2 cut(s) 235, 293
Bsh1285I CGRYCG 1 cut(s) 156
BsiEI CGRYCG 1 cut(s) 156
BsiSI CCGG 1 cut(s) 284
BslI CCNNNNNNNGG 2 cut(s) 240, 587
BsmAI GTCTC 1 cut(s) 292
BsmBI CGTCTC 1 cut(s) 292
BsmI GAATGC 1 cut(s) 688
Bsp119I TTCGAA 1 cut(s) 612
Bsp143I GATC 6 cut(s) 58, 226, 328, 519, 574, 640
BspACI CCGC 1 cut(s) 431
BspCNI CTCAG 1 cut(s) 687
BspLI GGNNCC 1 cut(s) 228
BspPI GGATC 2 cut(s) 221, 234
BspT104I TTCGAA 1 cut(s) 612
BssECI CCNNGG 1 cut(s) 117
BssMI GATC 6 cut(s) 58, 226, 328, 519, 574, 640
BssNI GRCGYC 1 cut(s) 301
Bst4CI ACNGT 3 cut(s) 154, 204, 526
Bst6I CTCTTC 1 cut(s) 264
BstACI GRCGYC 1 cut(s) 301
BstBI TTCGAA 1 cut(s) 612
BstC8I GCNNGC 1 cut(s) 291
BstDEI CTNAG 2 cut(s) 143, 674
BstF5I GGATG 2 cut(s) 127, 448
BstKTI GATC 6 cut(s) 61, 229, 331, 522, 577, 643
BstMAI GTCTC 1 cut(s) 292
BstMBI GATC 6 cut(s) 58, 226, 328, 519, 574, 640
BstMCI CGRYCG 1 cut(s) 156
BstMWI GCNNNNNNNGC 1 cut(s) 401
BstNSI RCATGY 1 cut(s) 543
BstX2I RGATCY 1 cut(s) 226
BstYI RGATCY 1 cut(s) 226
BsuI GTATCC 1 cut(s) 101
BtgZI GCGATG 1 cut(s) 95
BtsCI GGATG 2 cut(s) 127, 448
Cac8I GCNNGC 1 cut(s) 291
Csp6I GTAC 3 cut(s) 17, 46, 370
CviAII CATG 5 cut(s) 181, 263, 540, 572, 621
CviQI GTAC 3 cut(s) 17, 46, 370
DdeI CTNAG 2 cut(s) 143, 674
DpnI GATC 6 cut(s) 60, 228, 330, 521, 576, 642
DpnII GATC 6 cut(s) 58, 226, 328, 519, 574, 640
Eam1104I CTCTTC 1 cut(s) 264
EarI CTCTTC 1 cut(s) 264
EcoRI GAATTC 1 cut(s) 551
Esp3I CGTCTC 1 cut(s) 292
FaeI CATG 5 cut(s) 184, 266, 543, 575, 624
FalI AAGNNNNNCTT 2 cut(s) 161, 193
FatI CATG 5 cut(s) 180, 262, 539, 571, 620
FbaI TGATCA 1 cut(s) 519
FokI GGATG 2 cut(s) 134, 455
FspBI CTAG 6 cut(s) 6, 38, 438, 630, 647, 703
GsaI CCCAGC 2 cut(s) 239, 297
GsuI CTGGAG 1 cut(s) 550
HapII CCGG 1 cut(s) 284
Hin1I GRCGYC 1 cut(s) 301
Hin1II CATG 5 cut(s) 184, 266, 543, 575, 624
HincII GTYRAC 1 cut(s) 454
HindII GTYRAC 1 cut(s) 454
HinfI GANTC 3 cut(s) 434, 455, 482
HpaII CCGG 1 cut(s) 284
Hpy166II GTNNAC 2 cut(s) 200, 454
Hpy188I TCNGA 6 cut(s) 63, 118, 306, 322, 333, 677
Hpy188III TCNNGA 1 cut(s) 479
Hpy8I GTNNAC 2 cut(s) 200, 454
Hpy99I CGWCG 1 cut(s) 306
HpyAV CCTTC 3 cut(s) 7, 64, 187
HpyCH4III ACNGT 3 cut(s) 154, 204, 526
HpyCH4IV ACGT 4 cut(s) 48, 214, 301, 426
HpyCH4V TGCA 3 cut(s) 184, 262, 686
HpyF10VI GCNNNNNNNGC 1 cut(s) 401
HpyF3I CTNAG 2 cut(s) 143, 674
HpySE526I ACGT 4 cut(s) 48, 214, 301, 426
Hsp92I GRCGYC 1 cut(s) 301
Hsp92II CATG 5 cut(s) 184, 266, 543, 575, 624
Ksp22I TGATCA 1 cut(s) 519
Kzo9I GATC 6 cut(s) 58, 226, 328, 519, 574, 640
LmnI GCTCC 2 cut(s) 259, 286
MaeI CTAG 6 cut(s) 6, 38, 438, 630, 647, 703
MaeII ACGT 4 cut(s) 48, 214, 301, 426
MaeIII GTNAC 2 cut(s) 124, 623
MalI GATC 6 cut(s) 60, 228, 330, 521, 576, 642
MboI GATC 6 cut(s) 58, 226, 328, 519, 574, 640
MboII GAAGA 4 cut(s) 278, 281, 287, 635
MflI RGATCY 1 cut(s) 226
MluCI AATT 8 cut(s) 20, 358, 374, 406, 468, 510, 551, 608
MlyI GAGTC 1 cut(s) 449
MmeI TCCRAC 2 cut(s) 284, 605
MnlI CCTC 7 cut(s) 112, 201, 241, 265, 316, 439, 574
MseI TTAA 3 cut(s) 467, 515, 635
MslI CAYNNNNRTG 1 cut(s) 663
MspI CCGG 1 cut(s) 284
Mva1269I GAATGC 1 cut(s) 688
MwoI GCNNNNNNNGC 1 cut(s) 401
NdeII GATC 6 cut(s) 58, 226, 328, 519, 574, 640
NlaIII CATG 5 cut(s) 184, 266, 543, 575, 624
NlaIV GGNNCC 1 cut(s) 228
NspI RCATGY 1 cut(s) 543
NspV TTCGAA 1 cut(s) 612
OliI CACNNNNGTG 1 cut(s) 663
PciI ACATGT 1 cut(s) 539
PctI GAATGC 1 cut(s) 688
PfeI GAWTC 2 cut(s) 434, 482
PleI GAGTC 1 cut(s) 449
PpsI GAGTC 1 cut(s) 449
PscI ACATGT 1 cut(s) 539
Psp1406I AACGTT 1 cut(s) 214
PspFI CCCAGC 2 cut(s) 235, 293
PspN4I GGNNCC 1 cut(s) 228
PsuI RGATCY 1 cut(s) 226
RsaI GTAC 3 cut(s) 18, 47, 371
RsaNI GTAC 3 cut(s) 17, 46, 370
RseI CAYNNNNRTG 1 cut(s) 663
SaqAI TTAA 3 cut(s) 467, 515, 635
Sau3AI GATC 6 cut(s) 58, 226, 328, 519, 574, 640
SchI GAGTC 1 cut(s) 449
SfuI TTCGAA 1 cut(s) 612
SmiMI CAYNNNNRTG 1 cut(s) 663
SmlI CTYRAG 1 cut(s) 661
SmoI CTYRAG 1 cut(s) 661
Sse9I AATT 8 cut(s) 20, 358, 374, 406, 468, 510, 551, 608
SsiI CCGC 1 cut(s) 431
SspMI CTAG 6 cut(s) 6, 38, 438, 630, 647, 703
TaaI ACNGT 3 cut(s) 154, 204, 526
TaiI ACGT 4 cut(s) 51, 217, 304, 429
TaqI TCGA 2 cut(s) 156, 612
TasI AATT 8 cut(s) 20, 358, 374, 406, 468, 510, 551, 608
TfiI GAWTC 2 cut(s) 434, 482
Tru1I TTAA 3 cut(s) 467, 515, 635
Tru9I TTAA 3 cut(s) 467, 515, 635
TspDTI ATGAA 4 cut(s) 13, 169, 263, 279
TspGWI ACGGA 1 cut(s) 246
XapI RAATTY 4 cut(s) 374, 406, 551, 608
XceI RCATGY 1 cut(s) 543
XcmI CCANNNNNNNNNTGG 1 cut(s) 155
XspI CTAG 6 cut(s) 6, 38, 438, 630, 647, 703
ZraI GACGTC 1 cut(s) 302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.