pycom16g18540

60S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
N/A
Physical Location & Seq
Reverse (-)
15196616 .. 15198460
1845 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 612 bp
ATGTTTGGCTCTTCGGCCCTTGGTTGGAGATGGTTTTTTGTGACAGGAATATGCTTGCCCTGGCTCTCGGTTGGAGATGGATCGAACGGTCCACAGCATCCTACAAAGACCATAAGAAAAACCCTAATCCTCCGCTACCATTTTCTCTTCTCTTCAAAGCTCCGCCTCCTCGGAAGTCCTCACACATCACACACTTCACCAGAACCAAAAATGCCTTTCAAGAGGTACGTCGAGATCGGAAGGGTGGCTCTCGTCAACTACGGCGAGGACTATGGGAAGCTCGTCGTCATCGTCGACGTCCTTGACCAGAACAGGGCTCTTGTCGATGCTCCTAATATGGTGAGGTCGCAGATGAACTTCAAGAGACTTTCACTCACTGACATCAAGATTGATATCAAGAGGGTGCCCAAGAAGAAGGAATTGCTTGCCGCAATGGAAGCTGCTGATGTGAAGAAGAAGTGGGAGAACAGCCCATGGGGTAGAAAACTGATTGTTCAGAAGAGAAGAGCATCTCTTAACGACTTTGACCGATTCAAGCTCATGTTGGCTAAGATTAAGAGGGCCGGACTGGTCAGGAGCGAGCTTGCAAAATTGAAAAAGGCTACAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L14e

Protein Analysis

204

Amino Acids

23.19

Weight (kDa)

10.42

Isoelectric Point (pI)

38.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L14e PF01929 115 - 187 9.3e-26 Ribosomal protein L14
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 300
AccB1I GGYRCC 1 cut(s) 403
AccI GTMKAC 1 cut(s) 294
AciI CCGC 3 cut(s) 133, 163, 429
AclWI GGATC 1 cut(s) 88
AcyI GRCGYC 1 cut(s) 297
AfaI GTAC 1 cut(s) 227
AfiI CCNNNNNNNGG 2 cut(s) 24, 313
AgsI TTSAA 5 cut(s) 156, 220, 361, 535, 595
AjnI CCWGG 1 cut(s) 59
AluBI AGCT 6 cut(s) 160, 280, 440, 538, 583, 608
AluI AGCT 6 cut(s) 160, 280, 440, 538, 583, 608
Alw26I GTCTC 1 cut(s) 358
AlwI GGATC 1 cut(s) 88
AoxI GGCC 2 cut(s) 15, 561
ApeKI GCWGC 1 cut(s) 440
AspS9I GGNCC 3 cut(s) 16, 89, 561
AsuHPI GGTGA 2 cut(s) 189, 352
AvaII GGWCC 1 cut(s) 89
BaeGI GKGCMC 1 cut(s) 408
BanI GGYRCC 1 cut(s) 403
BanII GRGCYC 1 cut(s) 319
BbvI GCAGC 1 cut(s) 427
BccI CCATC 2 cut(s) 24, 71
BceAI ACGGC 1 cut(s) 277
BciT130I CCWGG 1 cut(s) 61
BcoDI GTCTC 1 cut(s) 358
BfmI CTRYAG 1 cut(s) 603
BisI GCNGC 2 cut(s) 429, 441
BlsI GCNGC 2 cut(s) 430, 442
Bme1390I CCNGG 1 cut(s) 61
Bme18I GGWCC 1 cut(s) 89
BmgT120I GGNCC 3 cut(s) 16, 89, 561
BmiI GGNNCC 1 cut(s) 405
BmrFI CCNGG 1 cut(s) 61
BmsI GCATC 3 cut(s) 106, 316, 518
BsaBI GATNNNNATC 1 cut(s) 392
BsaHI GRCGYC 1 cut(s) 297
BsaJI CCNNGG 4 cut(s) 19, 59, 169, 473
Bsc4I CCNNNNNNNGG 2 cut(s) 24, 313
Bse1I ACTGG 1 cut(s) 573
Bse3DI GCAATG 1 cut(s) 438
Bse8I GATNNNNATC 1 cut(s) 392
BseBI CCWGG 1 cut(s) 61
BseDI CCNNGG 4 cut(s) 19, 59, 169, 473
BseGI GGATG 1 cut(s) 97
BseJI GATNNNNATC 1 cut(s) 392
BseLI CCNNNNNNNGG 2 cut(s) 24, 313
BseMI GCAATG 1 cut(s) 438
BseNI ACTGG 1 cut(s) 573
BseRI GAGGAG 1 cut(s) 158
BseSI GKGCMC 1 cut(s) 408
BseXI GCAGC 1 cut(s) 427
BshFI GGCC 2 cut(s) 17, 563
BshNI GGYRCC 1 cut(s) 403
BsiSI CCGG 1 cut(s) 564
BslI CCNNNNNNNGG 2 cut(s) 24, 313
BsmAI GTCTC 1 cut(s) 358
BsnI GGCC 2 cut(s) 17, 563
Bsp1286I GDGCHC 2 cut(s) 319, 408
Bsp143I GATC 2 cut(s) 80, 234
Bsp19I CCATGG 1 cut(s) 473
BspACI CCGC 3 cut(s) 133, 163, 429
BspANI GGCC 2 cut(s) 17, 563
BspLI GGNNCC 1 cut(s) 405
BspPI GGATC 1 cut(s) 88
BspQI GCTCTTC 2 cut(s) 16, 499
BspT107I GGYRCC 1 cut(s) 403
BsrDI GCAATG 1 cut(s) 438
BsrI ACTGG 1 cut(s) 573
BssECI CCNNGG 4 cut(s) 19, 59, 169, 473
BssMI GATC 2 cut(s) 80, 234
BssNI GRCGYC 1 cut(s) 297
BssT1I CCWWGG 2 cut(s) 19, 473
Bst2UI CCWGG 1 cut(s) 61
Bst4CI ACNGT 1 cut(s) 89
Bst6I CTCTTC 5 cut(s) 16, 152, 157, 494, 499
BstACI GRCGYC 1 cut(s) 297
BstC8I GCNNGC 4 cut(s) 56, 426, 581, 585
BstDEI CTNAG 1 cut(s) 549
BstDSI CCRYGG 1 cut(s) 473
BstF5I GGATG 1 cut(s) 97
BstKTI GATC 2 cut(s) 83, 237
BstMAI GTCTC 1 cut(s) 358
BstMBI GATC 2 cut(s) 80, 234
BstMWI GCNNNNNNNGC 1 cut(s) 437
BstNI CCWGG 1 cut(s) 61
BstSCI CCNGG 1 cut(s) 59
BstSFI CTRYAG 1 cut(s) 603
BstSLI GKGCMC 1 cut(s) 408
BstV1I GCAGC 1 cut(s) 427
BsuRI GGCC 2 cut(s) 17, 563
BtgI CCRYGG 1 cut(s) 473
BtsCI GGATG 1 cut(s) 97
BtsIMutI CAGTG 1 cut(s) 375
Cac8I GCNNGC 4 cut(s) 56, 426, 581, 585
Cfr13I GGNCC 3 cut(s) 16, 89, 561
Csp6I GTAC 1 cut(s) 226
CviAII CATG 2 cut(s) 474, 541
CviQI GTAC 1 cut(s) 226
DdeI CTNAG 1 cut(s) 549
DpnI GATC 2 cut(s) 82, 236
DpnII GATC 2 cut(s) 80, 234
Eam1104I CTCTTC 5 cut(s) 16, 152, 157, 494, 499
EarI CTCTTC 5 cut(s) 16, 152, 157, 494, 499
EciI GGCGGA 1 cut(s) 152
Eco130I CCWWGG 2 cut(s) 19, 473
Eco24I GRGCYC 1 cut(s) 319
Eco32I GATATC 1 cut(s) 394
Eco47I GGWCC 1 cut(s) 89
EcoRII CCWGG 1 cut(s) 59
EcoRV GATATC 1 cut(s) 394
EcoT14I CCWWGG 2 cut(s) 19, 473
EcoT38I GRGCYC 1 cut(s) 319
ErhI CCWWGG 2 cut(s) 19, 473
FaeI CATG 2 cut(s) 477, 544
FaiI YATR 6 cut(s) 52, 113, 273, 338, 475, 542
FatI CATG 2 cut(s) 473, 540
FblI GTMKAC 1 cut(s) 294
Fnu4HI GCNGC 2 cut(s) 429, 441
FokI GGATG 1 cut(s) 84
FriOI GRGCYC 1 cut(s) 319
Fsp4HI GCNGC 2 cut(s) 429, 441
GluI GCNGC 2 cut(s) 429, 441
HaeIII GGCC 2 cut(s) 17, 563
HapII CCGG 1 cut(s) 564
Hin1I GRCGYC 1 cut(s) 297
Hin1II CATG 2 cut(s) 477, 544
HincII GTYRAC 2 cut(s) 256, 295
HindII GTYRAC 2 cut(s) 256, 295
HinfI GANTC 1 cut(s) 531
HpaII CCGG 1 cut(s) 564
HphI GGTGA 2 cut(s) 189, 352
Hpy166II GTNNAC 3 cut(s) 92, 256, 295
Hpy188I TCNGA 3 cut(s) 173, 239, 498
Hpy188III TCNNGA 6 cut(s) 220, 232, 361, 385, 397, 574
Hpy8I GTNNAC 3 cut(s) 92, 256, 295
Hpy99I CGWCG 4 cut(s) 233, 287, 296, 299
HpyAV CCTTC 2 cut(s) 234, 409
HpyCH4III ACNGT 1 cut(s) 89
HpyCH4IV ACGT 2 cut(s) 228, 297
HpyCH4V TGCA 1 cut(s) 587
HpyF10VI GCNNNNNNNGC 1 cut(s) 437
HpyF3I CTNAG 1 cut(s) 549
HpySE526I ACGT 2 cut(s) 228, 297
Hsp92I GRCGYC 1 cut(s) 297
Hsp92II CATG 2 cut(s) 477, 544
Kzo9I GATC 2 cut(s) 80, 234
LguI GCTCTTC 2 cut(s) 16, 499
LmnI GCTCC 3 cut(s) 165, 334, 576
LpnPI CCDG 9 cut(s) 30, 46, 73, 213, 298, 320, 554, 559, 577
Lsp1109I GCAGC 1 cut(s) 427
LweI GCATC 3 cut(s) 106, 316, 518
MaeII ACGT 2 cut(s) 228, 297
MaeIII GTNAC 1 cut(s) 40
MalI GATC 2 cut(s) 82, 236
MboI GATC 2 cut(s) 80, 234
MboII GAAGA 8 cut(s) 3, 139, 144, 424, 463, 466, 511, 516
MhlI GDGCHC 2 cut(s) 319, 408
MluCI AATT 2 cut(s) 419, 590
MmeI TCCRAC 2 cut(s) 5, 52
MnlI CCTC 9 cut(s) 140, 176, 179, 189, 216, 259, 336, 393, 552
MseI TTAA 3 cut(s) 516, 555, 610
MspI CCGG 1 cut(s) 564
MspR9I CCNGG 1 cut(s) 61
MvaI CCWGG 1 cut(s) 61
MwoI GCNNNNNNNGC 1 cut(s) 437
NcoI CCATGG 1 cut(s) 473
NdeII GATC 2 cut(s) 80, 234
NlaIII CATG 2 cut(s) 477, 544
NlaIV GGNNCC 1 cut(s) 405
NmuCI GTSAC 1 cut(s) 40
PciSI GCTCTTC 2 cut(s) 16, 499
PcsI WCGNNNNNNNCGW 2 cut(s) 288, 291
PfeI GAWTC 1 cut(s) 531
PkrI GCNGC 2 cut(s) 430, 442
Psp6I CCWGG 1 cut(s) 59
PspGI CCWGG 1 cut(s) 59
PspN4I GGNNCC 1 cut(s) 405
PspPI GGNCC 3 cut(s) 16, 89, 561
RsaI GTAC 1 cut(s) 227
RsaNI GTAC 1 cut(s) 226
SalI GTCGAC 1 cut(s) 293
SapI GCTCTTC 2 cut(s) 16, 499
SaqAI TTAA 3 cut(s) 516, 555, 610
SatI GCNGC 2 cut(s) 429, 441
Sau3AI GATC 2 cut(s) 80, 234
Sau96I GGNCC 3 cut(s) 16, 89, 561
ScrFI CCNGG 1 cut(s) 61
SduI GDGCHC 2 cut(s) 319, 408
SfaNI GCATC 3 cut(s) 106, 316, 518
SfcI CTRYAG 1 cut(s) 603
SgrDI CGTCGACG 1 cut(s) 293
SinI GGWCC 1 cut(s) 89
Sse9I AATT 2 cut(s) 419, 590
SsiI CCGC 3 cut(s) 133, 163, 429
StyD4I CCNGG 1 cut(s) 59
StyI CCWWGG 2 cut(s) 19, 473
TaaI ACNGT 1 cut(s) 89
TaiI ACGT 2 cut(s) 231, 300
TaqI TCGA 4 cut(s) 83, 231, 294, 324
TaqII GACCGA 1 cut(s) 543
TasI AATT 2 cut(s) 419, 590
TauI GCSGC 1 cut(s) 431
TfiI GAWTC 1 cut(s) 531
Tru1I TTAA 3 cut(s) 516, 555, 610
Tru9I TTAA 3 cut(s) 516, 555, 610
TscAI CASTG 1 cut(s) 382
TseFI GTSAC 1 cut(s) 40
TseI GCWGC 1 cut(s) 440
Tsp45I GTSAC 1 cut(s) 40
TspDTI ATGAA 1 cut(s) 368
TspRI CASTG 1 cut(s) 382
VpaK11BI GGWCC 1 cut(s) 89
XmiI GTMKAC 1 cut(s) 294
ZraI GACGTC 1 cut(s) 298
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.