pycom16g19550

Protein of unknown function (DUF3537)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
N/A
Physical Location & Seq
Reverse (-)
16687704 .. 16689086
1383 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 621 bp
ATGGCCACGACCACGAAGAGGAGACGTTGTTGGCGATATCGAAGCTCCCAAGGAATTGACCGAGGAGGAGGACATGAAAGTACAGATTTGGATTTCATCAGCACGACCAAGATCATCAGCATTTTGATTGTCTGCAGCTCTCAGTTCTTGGTCTCCACAATTGTTCAGGTTGTTGGCATTATTCTTTCCTTGCACACAGCTACTAAAATTTCCAGTAGAGACCAAGGTATAGCGACATTTGCCAGTAGATGGCATGCGTTAATGACATGCAGTTCTAATGATTCACCGCATAAGAGAAGTTCAGACAGTTTGGTGAACTTTGAGGGTGCCAACAGATTGAGCTCACTGCATACAAGTTACTCTCAGAGTGATTTGGAGTCAACGGATTATGTTCATGTGCCTACAAATTCCCAGCTGGCTTCACATATGGCTTCATACCACAAGAGACAAGCTTTTGGTATGCGTCAGCTTATGCTGTTTCAGTTTTTTCTGTTTATCATTTTTGTGTTTGAAAAGATAGCTCTTTTGGTTGTCATTTATGGTTTTGGTTTGTTCATTTCCCGTGGTTCAGTATTACTGGTTGTTGATTGTGGCTCATACTCTATTTATATTTCTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3537

Protein Analysis

207

Amino Acids

23.05

Weight (kDa)

9.32

Isoelectric Point (pI)

48.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3537 PF12056 39 - 152 7.7e-24 Protein of unknown function (DUF3537)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 326
AccB7I CCANNNNNTGG 1 cut(s) 249
AciI CCGC 1 cut(s) 287
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 207, 406
AfaI GTAC 1 cut(s) 82
AfiI CCNNNNNNNGG 2 cut(s) 18, 249
AgsI TTSAA 1 cut(s) 512
AluBI AGCT 8 cut(s) 45, 138, 200, 342, 415, 452, 469, 521
AluI AGCT 8 cut(s) 45, 138, 200, 342, 415, 452, 469, 521
Alw21I GWGCWC 1 cut(s) 344
Alw26I GTCTC 4 cut(s) 16, 157, 213, 439
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 1 cut(s) 135
ApoI RAATTY 2 cut(s) 207, 406
AsuHPI GGTGA 2 cut(s) 276, 325
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 326
BanII GRGCYC 1 cut(s) 344
Bbv12I GWGCWC 1 cut(s) 344
BbvI GCAGC 1 cut(s) 147
BccI CCATC 1 cut(s) 243
BcoDI GTCTC 4 cut(s) 16, 157, 213, 439
BfmI CTRYAG 1 cut(s) 133
BisI GCNGC 1 cut(s) 136
BlsI GCNGC 1 cut(s) 137
BmiI GGNNCC 1 cut(s) 328
BsaI GGTCTC 2 cut(s) 157, 213
BsaJI CCNNGG 4 cut(s) 49, 61, 223, 562
BsaXI ACNNNNNCTCC 2 cut(s) 13, 43
Bsc4I CCNNNNNNNGG 2 cut(s) 18, 249
Bse1I ACTGG 3 cut(s) 213, 243, 582
BseDI CCNNGG 4 cut(s) 49, 61, 223, 562
BseLI CCNNNNNNNGG 2 cut(s) 18, 249
BseMII CTCAG 2 cut(s) 155, 377
BseNI ACTGG 3 cut(s) 213, 243, 582
BseRI GAGGAG 3 cut(s) 34, 78, 81
BseXI GCAGC 1 cut(s) 147
BseYI CCCAGC 1 cut(s) 411
BshFI GGCC 1 cut(s) 5
BshNI GGYRCC 1 cut(s) 326
BsiHKAI GWGCWC 1 cut(s) 344
BslI CCNNNNNNNGG 2 cut(s) 18, 249
BsmAI GTCTC 4 cut(s) 16, 157, 213, 439
BsmBI CGTCTC 1 cut(s) 16
BsnI GGCC 1 cut(s) 5
Bso31I GGTCTC 2 cut(s) 157, 213
Bsp1286I GDGCHC 1 cut(s) 344
Bsp143I GATC 1 cut(s) 111
BspACI CCGC 1 cut(s) 287
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 2 cut(s) 154, 376
BspLI GGNNCC 1 cut(s) 328
BspMAI CTGCAG 1 cut(s) 137
BspT107I GGYRCC 1 cut(s) 326
BspTNI GGTCTC 2 cut(s) 157, 213
BsrI ACTGG 3 cut(s) 213, 243, 582
BssECI CCNNGG 4 cut(s) 49, 61, 223, 562
BssMI GATC 1 cut(s) 111
BssT1I CCWWGG 2 cut(s) 49, 223
Bst4CI ACNGT 1 cut(s) 308
Bst6I CTCTTC 1 cut(s) 11
BstC8I GCNNGC 2 cut(s) 255, 417
BstDEI CTNAG 2 cut(s) 141, 363
BstDSI CCRYGG 1 cut(s) 562
BstKTI GATC 1 cut(s) 114
BstMAI GTCTC 4 cut(s) 16, 157, 213, 439
BstMBI GATC 1 cut(s) 111
BstMWI GCNNNNNNNGC 1 cut(s) 239
BstNSI RCATGY 2 cut(s) 257, 270
BstSFI CTRYAG 1 cut(s) 133
BstV1I GCAGC 1 cut(s) 147
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 562
BtsI GCAGTG 1 cut(s) 344
BtsIMutI CAGTG 1 cut(s) 344
Cac8I GCNNGC 2 cut(s) 255, 417
CseI GACGC 1 cut(s) 452
Csp6I GTAC 1 cut(s) 81
CviAII CATG 4 cut(s) 74, 254, 267, 395
CviQI GTAC 1 cut(s) 81
DdeI CTNAG 2 cut(s) 141, 363
DpnI GATC 1 cut(s) 113
DpnII GATC 1 cut(s) 111
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 11
EarI CTCTTC 1 cut(s) 11
Ecl136II GAGCTC 1 cut(s) 342
Eco130I CCWWGG 2 cut(s) 49, 223
Eco24I GRGCYC 1 cut(s) 344
Eco31I GGTCTC 2 cut(s) 157, 213
Eco32I GATATC 1 cut(s) 38
Eco53kI GAGCTC 1 cut(s) 342
EcoICRI GAGCTC 1 cut(s) 342
EcoRV GATATC 1 cut(s) 38
EcoT14I CCWWGG 2 cut(s) 49, 223
EcoT38I GRGCYC 1 cut(s) 344
ErhI CCWWGG 2 cut(s) 49, 223
Esp3I CGTCTC 1 cut(s) 16
FaeI CATG 4 cut(s) 77, 257, 270, 398
FatI CATG 4 cut(s) 73, 253, 266, 394
FauNDI CATATG 1 cut(s) 426
Fnu4HI GCNGC 1 cut(s) 136
FriOI GRGCYC 1 cut(s) 344
Fsp4HI GCNGC 1 cut(s) 136
GluI GCNGC 1 cut(s) 136
GsaI CCCAGC 1 cut(s) 415
HaeIII GGCC 1 cut(s) 5
HgaI GACGC 1 cut(s) 452
Hin1II CATG 4 cut(s) 77, 257, 270, 398
HincII GTYRAC 1 cut(s) 381
HindII GTYRAC 1 cut(s) 381
HindIII AAGCTT 1 cut(s) 450
HinfI GANTC 2 cut(s) 281, 377
HphI GGTGA 2 cut(s) 276, 325
Hpy166II GTNNAC 2 cut(s) 316, 381
Hpy188I TCNGA 2 cut(s) 304, 366
Hpy8I GTNNAC 2 cut(s) 316, 381
HpyCH4III ACNGT 1 cut(s) 308
HpyCH4IV ACGT 1 cut(s) 25
HpyCH4V TGCA 4 cut(s) 135, 193, 270, 349
HpyF10VI GCNNNNNNNGC 1 cut(s) 239
HpyF3I CTNAG 2 cut(s) 141, 363
HpySE526I ACGT 1 cut(s) 25
Hsp92II CATG 4 cut(s) 77, 257, 270, 398
Kzo9I GATC 1 cut(s) 111
LmnI GCTCC 1 cut(s) 50
LpnPI CCDG 6 cut(s) 152, 226, 256, 401, 425, 563
Lsp1109I GCAGC 1 cut(s) 147
MaeII ACGT 1 cut(s) 25
MaeIII GTNAC 1 cut(s) 356
MalI GATC 1 cut(s) 113
MboI GATC 1 cut(s) 111
MboII GAAGA 1 cut(s) 28
MfeI CAATTG 1 cut(s) 159
MhlI GDGCHC 1 cut(s) 344
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 4 cut(s) 54, 159, 207, 406
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 386
MnlI CCTC 5 cut(s) 12, 56, 59, 62, 316
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 260
MslI CAYNNNNRTG 1 cut(s) 503
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 415
MunI CAATTG 1 cut(s) 159
MwoI GCNNNNNNNGC 1 cut(s) 239
NdeI CATATG 1 cut(s) 426
NdeII GATC 1 cut(s) 111
NlaIII CATG 4 cut(s) 77, 257, 270, 398
NlaIV GGNNCC 1 cut(s) 328
NspI RCATGY 2 cut(s) 257, 270
PaeI GCATGC 1 cut(s) 257
PcsI WCGNNNNNNNCGW 1 cut(s) 31
PfeI GAWTC 1 cut(s) 281
PflMI CCANNNNNTGG 1 cut(s) 249
PkrI GCNGC 1 cut(s) 137
PleI GAGTC 1 cut(s) 385
PpsI GAGTC 1 cut(s) 385
Psp124BI GAGCTC 1 cut(s) 344
PspFI CCCAGC 1 cut(s) 411
PspN4I GGNNCC 1 cut(s) 328
PstI CTGCAG 1 cut(s) 137
PvuII CAGCTG 1 cut(s) 415
RsaI GTAC 1 cut(s) 82
RsaNI GTAC 1 cut(s) 81
RseI CAYNNNNRTG 1 cut(s) 503
SacI GAGCTC 1 cut(s) 344
SaqAI TTAA 1 cut(s) 260
SatI GCNGC 1 cut(s) 136
Sau3AI GATC 1 cut(s) 111
SchI GAGTC 1 cut(s) 386
SduI GDGCHC 1 cut(s) 344
SfcI CTRYAG 1 cut(s) 133
SmiMI CAYNNNNRTG 1 cut(s) 503
SphI GCATGC 1 cut(s) 257
Sse9I AATT 4 cut(s) 54, 159, 207, 406
SsiI CCGC 1 cut(s) 287
SstI GAGCTC 1 cut(s) 344
StyI CCWWGG 2 cut(s) 49, 223
TaaI ACNGT 1 cut(s) 308
TaiI ACGT 1 cut(s) 28
TaqI TCGA 1 cut(s) 40
TaqII GACCGA 1 cut(s) 75
TasI AATT 4 cut(s) 54, 159, 207, 406
TatI WGTACW 1 cut(s) 80
TfiI GAWTC 1 cut(s) 281
Tru1I TTAA 1 cut(s) 260
Tru9I TTAA 1 cut(s) 260
TscAI CASTG 1 cut(s) 351
TseI GCWGC 1 cut(s) 135
TspDTI ATGAA 5 cut(s) 85, 90, 383, 423, 544
TspGWI ACGGA 1 cut(s) 398
TspRI CASTG 1 cut(s) 351
Van91I CCANNNNNTGG 1 cut(s) 249
XapI RAATTY 2 cut(s) 207, 406
XceI RCATGY 2 cut(s) 257, 270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.