pycom16g19770

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
N/A
Physical Location & Seq
Reverse (-)
17054948 .. 17056003
1056 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 615 bp
ATGAGCAAAGAGCATGCTTTCGAAACTTTACCAGTGCCTAGCCAAAGCACATATCCTCGTCGAATAGCTGTTAATGATCCTTCAATGATTCTAATTGTTGGGGACTTAACTTATGCAAATCAGTACCTTACAACTGGCGGGAAGGGAGCTTCATGCTATTCACGTGCATTCCCAGATGCACCAATCAGAGAAACATATCAACCTCGTTGGGACGGGTGGGGAAGGTTCATGGAGCCCTTAACCTCGAGAGTTCCTATGATGGTTATTGAAGGCAACCATGAGATTGAGCCTCAAGTTTCTGGAATCACCTTCCAGTCCTATTTGACAAGATTCACACGTAAGCACTTTCATAACCGGTGTACTTTTTTGGAATCTGTTGTTTTTAATTGTTTGTTATGTCCTATTAATGTTCATTCAGGTGCTCAATATGCTTGGCTTAAAGATGATCTGCATCGAGTAGACTGCATAATGACCCCCTGGCTCGTAGCTGCATGGCACTCTCCTTGGTATAACAGCTATTCTTCACACCATCAGGAATTTGGATGCATGAGACAAGAAATGGAATCACTTCTTTATCAATATGGTGTTGATATGGTTTTTTTTCTAGTCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

205

Amino Acids

23.7

Weight (kDa)

6.29

Isoelectric Point (pI)

53.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 22 - 201 1.4e-11 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 459
AciI CCGC 1 cut(s) 138
AclWI GGATC 1 cut(s) 71
AcsI RAATTY 1 cut(s) 536
AcvI CACGTG 1 cut(s) 164
AfaI GTAC 2 cut(s) 125, 361
AflIII ACRYGT 1 cut(s) 335
AgeI ACCGGT 1 cut(s) 354
AgsI TTSAA 2 cut(s) 84, 269
AjnI CCWGG 1 cut(s) 476
AluBI AGCT 4 cut(s) 68, 149, 488, 516
AluI AGCT 4 cut(s) 68, 149, 488, 516
Alw21I GWGCWC 1 cut(s) 424
Alw26I GTCTC 1 cut(s) 544
AlwI GGATC 1 cut(s) 71
Ama87I CYCGRG 1 cut(s) 244
ApeKI GCWGC 1 cut(s) 488
ApoI RAATTY 1 cut(s) 536
AseI ATTAAT 1 cut(s) 405
AsiGI ACCGGT 1 cut(s) 354
Asp700I GAANNNNTTC 1 cut(s) 567
AsuHPI GGTGA 1 cut(s) 298
AsuII TTCGAA 1 cut(s) 21
AvaI CYCGRG 1 cut(s) 244
BanII GRGCYC 1 cut(s) 237
BbrPI CACGTG 1 cut(s) 164
Bbv12I GWGCWC 1 cut(s) 424
BbvI GCAGC 1 cut(s) 475
BccI CCATC 2 cut(s) 253, 537
BcgI CGANNNNNNTGC 2 cut(s) 444, 478
BciT130I CCWGG 1 cut(s) 478
BcoDI GTCTC 1 cut(s) 544
BfaI CTAG 2 cut(s) 39, 605
BisI GCNGC 1 cut(s) 489
BlsI GCNGC 1 cut(s) 490
Bme1390I CCNGG 1 cut(s) 478
BmeT110I CYCGRG 1 cut(s) 244
BmiI GGNNCC 1 cut(s) 234
BmrFI CCNGG 1 cut(s) 478
BmsI GCATC 3 cut(s) 166, 460, 533
Bpu14I TTCGAA 1 cut(s) 21
BpuEI CTTGAG 1 cut(s) 276
BsaAI YACGTR 2 cut(s) 164, 338
BsaBI GATNNNNATC 1 cut(s) 450
BsaJI CCNNGG 2 cut(s) 476, 503
BsaWI WCCGGW 1 cut(s) 354
Bse118I RCCGGY 1 cut(s) 354
Bse1I ACTGG 3 cut(s) 32, 139, 313
Bse8I GATNNNNATC 1 cut(s) 450
BseBI CCWGG 1 cut(s) 478
BseDI CCNNGG 2 cut(s) 476, 503
BseGI GGATG 1 cut(s) 548
BseJI GATNNNNATC 1 cut(s) 450
BseNI ACTGG 3 cut(s) 32, 139, 313
BseXI GCAGC 1 cut(s) 475
BshTI ACCGGT 1 cut(s) 354
BsiHKAI GWGCWC 1 cut(s) 424
BsiHKCI CYCGRG 1 cut(s) 244
BsiSI CCGG 1 cut(s) 355
BslFI GGGAC 2 cut(s) 116, 224
BsmAI GTCTC 1 cut(s) 544
BsmFI GGGAC 2 cut(s) 116, 224
BsmI GAATGC 1 cut(s) 167
BsoBI CYCGRG 1 cut(s) 244
Bsp119I TTCGAA 1 cut(s) 21
Bsp1286I GDGCHC 2 cut(s) 237, 424
Bsp143I GATC 2 cut(s) 76, 445
BspACI CCGC 1 cut(s) 138
BspLI GGNNCC 1 cut(s) 234
BspPI GGATC 1 cut(s) 71
BspT104I TTCGAA 1 cut(s) 21
BsrFI RCCGGY 1 cut(s) 354
BsrI ACTGG 3 cut(s) 32, 139, 313
BssAI RCCGGY 1 cut(s) 354
BssECI CCNNGG 2 cut(s) 476, 503
BssMI GATC 2 cut(s) 76, 445
BssT1I CCWWGG 1 cut(s) 503
Bst2UI CCWGG 1 cut(s) 478
BstBAI YACGTR 2 cut(s) 164, 338
BstBI TTCGAA 1 cut(s) 21
BstC8I GCNNGC 1 cut(s) 15
BstF5I GGATG 1 cut(s) 548
BstKTI GATC 2 cut(s) 79, 448
BstMAI GTCTC 1 cut(s) 544
BstMBI GATC 2 cut(s) 76, 445
BstMWI GCNNNNNNNGC 1 cut(s) 428
BstNI CCWGG 1 cut(s) 478
BstNSI RCATGY 1 cut(s) 17
BstSCI CCNGG 1 cut(s) 476
BstV1I GCAGC 1 cut(s) 475
BtsCI GGATG 1 cut(s) 548
BtsIMutI CAGTG 1 cut(s) 39
Cac8I GCNNGC 1 cut(s) 15
Cfr10I RCCGGY 1 cut(s) 354
Csp6I GTAC 2 cut(s) 124, 360
CspAI ACCGGT 1 cut(s) 354
CviAII CATG 7 cut(s) 14, 153, 229, 278, 492, 547, 610
CviJI RGCY 9 cut(s) 42, 68, 149, 235, 289, 436, 481, 488, 516
CviKI_1 RGCY 9 cut(s) 42, 68, 149, 235, 289, 436, 481, 488, 516
CviQI GTAC 2 cut(s) 124, 360
DpnI GATC 2 cut(s) 78, 447
DpnII GATC 2 cut(s) 76, 445
Eco130I CCWWGG 1 cut(s) 503
Eco24I GRGCYC 1 cut(s) 237
Eco72I CACGTG 1 cut(s) 164
Eco88I CYCGRG 1 cut(s) 244
EcoRII CCWGG 1 cut(s) 476
EcoT14I CCWWGG 1 cut(s) 503
EcoT22I ATGCAT 1 cut(s) 548
EcoT38I GRGCYC 1 cut(s) 237
ErhI CCWWGG 1 cut(s) 503
FaeI CATG 7 cut(s) 17, 156, 232, 281, 495, 550, 613
FaqI GGGAC 2 cut(s) 116, 224
FatI CATG 7 cut(s) 13, 152, 228, 277, 491, 546, 609
FauI CCCGC 1 cut(s) 131
FblI GTMKAC 1 cut(s) 459
Fnu4HI GCNGC 1 cut(s) 489
FokI GGATG 1 cut(s) 555
FriOI GRGCYC 1 cut(s) 237
Fsp4HI GCNGC 1 cut(s) 489
FspBI CTAG 2 cut(s) 39, 605
GluI GCNGC 1 cut(s) 489
HapII CCGG 1 cut(s) 355
Hin1II CATG 7 cut(s) 17, 156, 232, 281, 495, 550, 613
HinfI GANTC 5 cut(s) 88, 303, 330, 371, 563
HpaII CCGG 1 cut(s) 355
HphI GGTGA 1 cut(s) 298
Hpy166II GTNNAC 2 cut(s) 360, 460
Hpy188I TCNGA 1 cut(s) 188
Hpy188III TCNNGA 3 cut(s) 246, 300, 533
Hpy8I GTNNAC 2 cut(s) 360, 460
Hpy99I CGWCG 1 cut(s) 63
HpyAV CCTTC 5 cut(s) 90, 136, 216, 263, 319
HpyCH4IV ACGT 2 cut(s) 163, 337
HpyCH4V TGCA 7 cut(s) 116, 167, 179, 451, 465, 491, 546
HpyF10VI GCNNNNNNNGC 1 cut(s) 428
HpySE526I ACGT 2 cut(s) 163, 337
Hsp92II CATG 7 cut(s) 17, 156, 232, 281, 495, 550, 613
Kzo9I GATC 2 cut(s) 76, 445
LmnI GCTCC 2 cut(s) 146, 232
Lsp1109I GCAGC 1 cut(s) 475
LweI GCATC 3 cut(s) 166, 460, 533
MaeI CTAG 2 cut(s) 39, 605
MaeII ACGT 2 cut(s) 163, 337
MalI GATC 2 cut(s) 78, 447
MboI GATC 2 cut(s) 76, 445
MboII GAAGA 1 cut(s) 513
MhlI GDGCHC 2 cut(s) 237, 424
MluCI AATT 3 cut(s) 93, 385, 536
MnlI CCTC 4 cut(s) 66, 213, 253, 300
Mph1103I ATGCAT 1 cut(s) 548
MroXI GAANNNNTTC 1 cut(s) 567
MseI TTAA 6 cut(s) 72, 107, 239, 384, 405, 438
MslI CAYNNNNRTG 1 cut(s) 417
MspI CCGG 1 cut(s) 355
MspR9I CCNGG 1 cut(s) 478
Mva1269I GAATGC 1 cut(s) 167
MvaI CCWGG 1 cut(s) 478
MwoI GCNNNNNNNGC 1 cut(s) 428
NdeII GATC 2 cut(s) 76, 445
NlaIII CATG 7 cut(s) 17, 156, 232, 281, 495, 550, 613
NlaIV GGNNCC 1 cut(s) 234
NsiI ATGCAT 1 cut(s) 548
NspI RCATGY 1 cut(s) 17
NspV TTCGAA 1 cut(s) 21
PaeI GCATGC 1 cut(s) 17
PaeR7I CTCGAG 1 cut(s) 244
PctI GAATGC 1 cut(s) 167
PdmI GAANNNNTTC 1 cut(s) 567
PfeI GAWTC 5 cut(s) 88, 303, 330, 371, 563
PinAI ACCGGT 1 cut(s) 354
PkrI GCNGC 1 cut(s) 490
PmaCI CACGTG 1 cut(s) 164
PmlI CACGTG 1 cut(s) 164
Ppu21I YACGTR 2 cut(s) 164, 338
PshBI ATTAAT 1 cut(s) 405
Psp6I CCWGG 1 cut(s) 476
PspCI CACGTG 1 cut(s) 164
PspGI CCWGG 1 cut(s) 476
PspN4I GGNNCC 1 cut(s) 234
RsaI GTAC 2 cut(s) 125, 361
RsaNI GTAC 2 cut(s) 124, 360
RseI CAYNNNNRTG 1 cut(s) 417
SaqAI TTAA 6 cut(s) 72, 107, 239, 384, 405, 438
SatI GCNGC 1 cut(s) 489
Sau3AI GATC 2 cut(s) 76, 445
ScrFI CCNGG 1 cut(s) 478
SduI GDGCHC 2 cut(s) 237, 424
SfaNI GCATC 3 cut(s) 166, 460, 533
Sfr274I CTCGAG 1 cut(s) 244
SfuI TTCGAA 1 cut(s) 21
SlaI CTCGAG 1 cut(s) 244
SmiMI CAYNNNNRTG 1 cut(s) 417
SmlI CTYRAG 2 cut(s) 244, 291
SmoI CTYRAG 2 cut(s) 244, 291
SphI GCATGC 1 cut(s) 17
Sse9I AATT 3 cut(s) 93, 385, 536
SsiI CCGC 1 cut(s) 138
SspMI CTAG 2 cut(s) 39, 605
StyD4I CCNGG 1 cut(s) 476
StyI CCWWGG 1 cut(s) 503
TaiI ACGT 2 cut(s) 166, 340
TaqI TCGA 4 cut(s) 21, 61, 245, 454
TasI AATT 3 cut(s) 93, 385, 536
TatI WGTACW 1 cut(s) 359
TfiI GAWTC 5 cut(s) 88, 303, 330, 371, 563
Tru1I TTAA 6 cut(s) 72, 107, 239, 384, 405, 438
Tru9I TTAA 6 cut(s) 72, 107, 239, 384, 405, 438
TscAI CASTG 1 cut(s) 39
TseI GCWGC 1 cut(s) 488
TspDTI ATGAA 4 cut(s) 141, 217, 338, 401
TspRI CASTG 1 cut(s) 39
VspI ATTAAT 1 cut(s) 405
XapI RAATTY 1 cut(s) 536
XceI RCATGY 1 cut(s) 17
XcmI CCANNNNNNNNNTGG 1 cut(s) 536
XhoI CTCGAG 1 cut(s) 244
XmiI GTMKAC 1 cut(s) 459
XmnI GAANNNNTTC 1 cut(s) 567
XspI CTAG 2 cut(s) 39, 605
Zsp2I ATGCAT 1 cut(s) 548
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.