pycom16g21740

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
N/A
Physical Location & Seq
Forward (+)
20481689 .. 20484271
2583 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1053 bp
ATGAAAGGAATTGGAACCCCCCACCCACCCCGTGGTAACTTGTTATTTAATTCCAATATTTTTTCTCGTATTACTCAAAAACCAGAGGCTGTAACTATCAGTCCCTCAGGAAAATTCTTTGGCATCCGGAACAAGCGGAAGCTTCATATATGGAGAGTCCTTGCTAAAGACTCGAAACTCGTGATTGCTAAGAATTTTACTCTGCATCATACAAGAAACTTTAACGTTCTTGCATTTCATCCAACTCAGACAATTGTTGCCGCGGGTGATACAAGTGGGAGAATTCTAATCTGGACAGGATTTGGTAAAGGTACATTTTCTGATGGTGACAAAAGAATGAATGAAAGATCAATGAATAATGAGGATGAAAGGGATGGAGTTAGGGGAGATGACGATGCTGATGCTTCCTCCACATGGCATTGGCATGGTACTGGCATAAGTGTTCTTTCTTTCTCTTCTGACGGTGCCTATTTATATTCAGGTGGAATGGAAGGAGTTTTTGTGGTCTGGCAGCTAGACACAGGGAAAAAGAAGTTCTTGCGCAGAATTGTATCTCCTCTTCTATATTTGACTGATTCTCCAGACCTTTCACTTTCTTCTAATTCATATACTAAAAATGCCCTACATGGAAATCTTGGAAGTCTATTTCAGGGATCAAGCTTCCTTATTCAGGTCCAGAGATATACGATGGAATATGCAGCAAGTTTGGTTGCCTTGCGTACAGAGAATTATCACATCCAGTTCTACAGCTTGTTTGATGACCGTGAAATGTCTGAGGTTCAAATCTGTGAGAGAAATCATCAACCAGGAGATGAAGTCACAGTAATAGTGACTTTGGTGTCTCTATCTGGAGATGGCTCTATGATGAGCACAGTTGAAGTTAAGCTTCCTGAAGAGGGGTTAGGTGGTCTTGTTTGTCTTAAATTTTGGGCATCAGGGTCACAGAATAAAAGTTTCAGCCTGTCCACTGTTGTTTATGAACCTCACAGGGATGCTAGAATTTCTTCCCTTGCGTGCCATCCTACTCGTCGTATGGTTGCCAGCTCATCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
WD40

Protein Analysis

351

Amino Acids

38.88

Weight (kDa)

8.86

Isoelectric Point (pI)

35.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_CAF1B_HIR1 PF24105 47 - 178 3.3e-07 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR75_1st PF23869 136 - 206 2.6e-11 WD repeat-containing protein 75 first beta-propeller
Beta-prop_WDR75_2nd PF23769 239 - 342 4.1e-09 WD repeat-containing protein 75 second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 838
Acc16I TGCGCA 1 cut(s) 542
AccB1I GGYRCC 1 cut(s) 464
AccB7I CCANNNNNTGG 1 cut(s) 32
AccII CGCG 1 cut(s) 263
AccIII TCCGGA 1 cut(s) 126
AciI CCGC 3 cut(s) 136, 261, 263
AclI AACGTT 1 cut(s) 225
AclWI GGATC 1 cut(s) 661
AcsI RAATTY 5 cut(s) 113, 193, 282, 923, 999
AcuI CTGAAG 1 cut(s) 912
AdeI CACNNNGTG 1 cut(s) 32
AfaI GTAC 3 cut(s) 313, 430, 721
AfiI CCNNNNNNNGG 4 cut(s) 32, 414, 670, 896
AgsI TTSAA 2 cut(s) 782, 878
AjnI CCWGG 1 cut(s) 805
AluBI AGCT 6 cut(s) 142, 514, 660, 750, 886, 1044
AluI AGCT 6 cut(s) 142, 514, 660, 750, 886, 1044
Alw21I GWGCWC 1 cut(s) 872
Alw26I GTCTC 1 cut(s) 846
AlwI GGATC 1 cut(s) 661
AlwNI CAGNNNCTG 1 cut(s) 89
Aor13HI TCCGGA 1 cut(s) 126
ApeKI GCWGC 2 cut(s) 511, 698
ApoI RAATTY 5 cut(s) 113, 193, 282, 923, 999
Asp700I GAANNNNTTC 1 cut(s) 1003
AspLEI GCGC 1 cut(s) 543
AspS9I GGNCC 1 cut(s) 673
AsuHPI GGTGA 2 cut(s) 278, 338
AvaII GGWCC 1 cut(s) 673
AxyI CCTNAGG 1 cut(s) 106
BanI GGYRCC 1 cut(s) 464
BarI GAAGNNNNNNTAC 2 cut(s) 543, 575
BauI CACGAG 1 cut(s) 179
Bbv12I GWGCWC 1 cut(s) 872
BbvI GCAGC 2 cut(s) 523, 710
BccI CCATC 5 cut(s) 317, 368, 682, 848, 1026
BcgI CGANNNNNNTGC 2 cut(s) 383, 417
BciT130I CCWGG 1 cut(s) 807
BcoDI GTCTC 1 cut(s) 846
BfaI CTAG 2 cut(s) 515, 996
BfmI CTRYAG 1 cut(s) 745
BisI GCNGC 3 cut(s) 261, 512, 699
BlsI GCNGC 3 cut(s) 262, 513, 700
Bme1390I CCNGG 1 cut(s) 807
Bme18I GGWCC 1 cut(s) 673
BmgT120I GGNCC 1 cut(s) 673
BmiI GGNNCC 2 cut(s) 16, 466
BmrFI CCNGG 1 cut(s) 807
BmsI GCATC 6 cut(s) 132, 214, 385, 391, 941, 982
BpmI CTGGAG 2 cut(s) 564, 870
BsaJI CCNNGG 2 cut(s) 31, 261
BsaWI WCCGGW 1 cut(s) 126
BsaXI ACNNNNNCTCC 6 cut(s) 486, 516, 562, 592, 801, 831
Bsc4I CCNNNNNNNGG 4 cut(s) 32, 414, 670, 896
Bse1I ACTGG 2 cut(s) 436, 739
Bse21I CCTNAGG 1 cut(s) 106
BseAI TCCGGA 1 cut(s) 126
BseBI CCWGG 1 cut(s) 807
BseDI CCNNGG 2 cut(s) 31, 261
BseGI GGATG 8 cut(s) 123, 238, 370, 379, 735, 997, 1018, 1046
BseLI CCNNNNNNNGG 4 cut(s) 32, 414, 670, 896
BseMII CTCAG 3 cut(s) 120, 260, 765
BseNI ACTGG 2 cut(s) 436, 739
BseRI GAGGAG 1 cut(s) 546
BseXI GCAGC 2 cut(s) 523, 710
Bsh1236I CGCG 1 cut(s) 263
BshNI GGYRCC 1 cut(s) 464
BsiHKAI GWGCWC 1 cut(s) 872
BsiSI CCGG 1 cut(s) 127
BslFI GGGAC 1 cut(s) 87
BslI CCNNNNNNNGG 4 cut(s) 32, 414, 670, 896
BsmAI GTCTC 1 cut(s) 846
BsmFI GGGAC 1 cut(s) 87
Bsp1286I GDGCHC 1 cut(s) 872
Bsp13I TCCGGA 1 cut(s) 126
Bsp143I GATC 2 cut(s) 347, 653
BspACI CCGC 3 cut(s) 136, 261, 263
BspCNI CTCAG 3 cut(s) 119, 259, 766
BspEI TCCGGA 1 cut(s) 126
BspFNI CGCG 1 cut(s) 263
BspLI GGNNCC 2 cut(s) 16, 466
BspPI GGATC 1 cut(s) 661
BspT107I GGYRCC 1 cut(s) 464
BsrI ACTGG 2 cut(s) 436, 739
BssECI CCNNGG 2 cut(s) 31, 261
BssMI GATC 2 cut(s) 347, 653
BssSI CACGAG 1 cut(s) 179
Bst2BI CACGAG 1 cut(s) 179
Bst2UI CCWGG 1 cut(s) 807
Bst4CI ACNGT 5 cut(s) 464, 764, 823, 874, 970
Bst6I CTCTTC 3 cut(s) 460, 564, 888
BstC8I GCNNGC 2 cut(s) 1015, 1042
BstDEI CTNAG 4 cut(s) 106, 189, 246, 774
BstDSI CCRYGG 2 cut(s) 31, 261
BstENI CCTNNNNNAGG 1 cut(s) 668
BstF5I GGATG 8 cut(s) 123, 238, 370, 379, 735, 997, 1018, 1046
BstFNI CGCG 1 cut(s) 263
BstHHI GCGC 1 cut(s) 543
BstKTI GATC 2 cut(s) 350, 656
BstMAI GTCTC 1 cut(s) 846
BstMBI GATC 2 cut(s) 347, 653
BstNI CCWGG 1 cut(s) 807
BstSCI CCNGG 1 cut(s) 805
BstSFI CTRYAG 1 cut(s) 745
BstUI CGCG 1 cut(s) 263
BstV1I GCAGC 2 cut(s) 523, 710
Bsu36I CCTNAGG 1 cut(s) 106
BtgI CCRYGG 2 cut(s) 31, 261
BtsCI GGATG 8 cut(s) 123, 238, 370, 379, 735, 997, 1018, 1046
BtsIMutI CAGTG 1 cut(s) 966
Cac8I GCNNGC 2 cut(s) 1015, 1042
CaiI CAGNNNCTG 1 cut(s) 89
CfoI GCGC 1 cut(s) 543
Cfr13I GGNCC 1 cut(s) 673
Cfr42I CCGCGG 1 cut(s) 264
Csp6I GTAC 3 cut(s) 312, 429, 720
CviAII CATG 3 cut(s) 414, 425, 626
CviJI RGCY 9 cut(s) 89, 142, 514, 660, 750, 858, 886, 960, 1044
CviKI_1 RGCY 9 cut(s) 89, 142, 514, 660, 750, 858, 886, 960, 1044
CviQI GTAC 3 cut(s) 312, 429, 720
DdeI CTNAG 4 cut(s) 106, 189, 246, 774
DpnI GATC 2 cut(s) 349, 655
DpnII GATC 2 cut(s) 347, 653
DraIII CACNNNGTG 1 cut(s) 32
DrdI GACNNNNNNGTC 1 cut(s) 838
DseDI GACNNNNNNGTC 1 cut(s) 838
Eam1104I CTCTTC 3 cut(s) 460, 564, 888
EarI CTCTTC 3 cut(s) 460, 564, 888
Eco47I GGWCC 1 cut(s) 673
Eco57I CTGAAG 1 cut(s) 912
Eco81I CCTNAGG 1 cut(s) 106
EcoNI CCTNNNNNAGG 1 cut(s) 668
EcoRI GAATTC 1 cut(s) 282
EcoRII CCWGG 1 cut(s) 805
FaeI CATG 3 cut(s) 417, 428, 629
FalI AAGNNNNNCTT 4 cut(s) 521, 553, 870, 902
FaqI GGGAC 1 cut(s) 87
FatI CATG 3 cut(s) 413, 424, 625
FauI CCCGC 1 cut(s) 256
Fnu4HI GCNGC 3 cut(s) 261, 512, 699
FokI GGATG 8 cut(s) 110, 225, 377, 386, 722, 1004, 1005, 1033
Fsp4HI GCNGC 3 cut(s) 261, 512, 699
FspBI CTAG 2 cut(s) 515, 996
FspI TGCGCA 1 cut(s) 542
GlaI GCGC 1 cut(s) 542
GluI GCNGC 3 cut(s) 261, 512, 699
GsuI CTGGAG 2 cut(s) 564, 870
HapII CCGG 1 cut(s) 127
HhaI GCGC 1 cut(s) 543
Hin1II CATG 3 cut(s) 417, 428, 629
Hin6I GCGC 1 cut(s) 541
HinP1I GCGC 1 cut(s) 541
HindIII AAGCTT 3 cut(s) 140, 658, 884
HinfI GANTC 3 cut(s) 156, 170, 575
HpaII CCGG 1 cut(s) 127
HphI GGTGA 2 cut(s) 278, 338
Hpy166II GTNNAC 1 cut(s) 966
Hpy188I TCNGA 4 cut(s) 249, 322, 460, 775
Hpy188III TCNNGA 8 cut(s) 108, 127, 181, 292, 581, 676, 849, 890
Hpy8I GTNNAC 1 cut(s) 966
Hpy99I CGWCG 1 cut(s) 1032
HpyAV CCTTC 1 cut(s) 485
HpyCH4III ACNGT 5 cut(s) 464, 764, 823, 874, 970
HpyCH4IV ACGT 1 cut(s) 225
HpyCH4V TGCA 3 cut(s) 205, 233, 698
HpyF3I CTNAG 4 cut(s) 106, 189, 246, 774
HpySE526I ACGT 1 cut(s) 225
Hsp92II CATG 3 cut(s) 417, 428, 629
HspAI GCGC 1 cut(s) 541
Kpn2I TCCGGA 1 cut(s) 126
KspI CCGCGG 1 cut(s) 264
Kzo9I GATC 2 cut(s) 347, 653
Lsp1109I GCAGC 2 cut(s) 523, 710
LweI GCATC 6 cut(s) 132, 214, 385, 391, 941, 982
MaeI CTAG 2 cut(s) 515, 996
MaeII ACGT 1 cut(s) 225
MaeIII GTNAC 6 cut(s) 35, 91, 326, 817, 829, 939
MalI GATC 2 cut(s) 349, 655
MboI GATC 2 cut(s) 347, 653
MboII GAAGA 5 cut(s) 447, 551, 588, 905, 996
MfeI CAATTG 1 cut(s) 252
MhlI GDGCHC 1 cut(s) 872
MlyI GAGTC 2 cut(s) 164, 165
MmeI TCCRAC 1 cut(s) 266
MnlI CCTC 8 cut(s) 79, 115, 355, 418, 567, 769, 889, 993
MroI TCCGGA 1 cut(s) 126
MroXI GAANNNNTTC 1 cut(s) 1003
MseI TTAA 4 cut(s) 48, 222, 882, 921
MslI CAYNNNNRTG 2 cut(s) 423, 990
MspA1I CMGCKG 1 cut(s) 263
MspI CCGG 1 cut(s) 127
MspR9I CCNGG 1 cut(s) 807
MunI CAATTG 1 cut(s) 252
MvaI CCWGG 1 cut(s) 807
MvnI CGCG 1 cut(s) 263
NdeII GATC 2 cut(s) 347, 653
NlaIII CATG 3 cut(s) 417, 428, 629
NlaIV GGNNCC 2 cut(s) 16, 466
NmuCI GTSAC 4 cut(s) 326, 817, 829, 939
NsbI TGCGCA 1 cut(s) 542
PdmI GAANNNNTTC 1 cut(s) 1003
PfeI GAWTC 1 cut(s) 575
PflMI CCANNNNNTGG 1 cut(s) 32
PkrI GCNGC 3 cut(s) 262, 513, 700
PleI GAGTC 2 cut(s) 164, 164
PpsI GAGTC 2 cut(s) 164, 164
Psp1406I AACGTT 1 cut(s) 225
Psp6I CCWGG 1 cut(s) 805
PspGI CCWGG 1 cut(s) 805
PspN4I GGNNCC 2 cut(s) 16, 466
PspPI GGNCC 1 cut(s) 673
PstNI CAGNNNCTG 1 cut(s) 89
RsaI GTAC 3 cut(s) 313, 430, 721
RsaNI GTAC 3 cut(s) 312, 429, 720
RseI CAYNNNNRTG 2 cut(s) 423, 990
SacII CCGCGG 1 cut(s) 264
SaqAI TTAA 4 cut(s) 48, 222, 882, 921
SatI GCNGC 3 cut(s) 261, 512, 699
Sau3AI GATC 2 cut(s) 347, 653
Sau96I GGNCC 1 cut(s) 673
SchI GAGTC 2 cut(s) 164, 165
ScrFI CCNGG 1 cut(s) 807
SduI GDGCHC 1 cut(s) 872
SfaNI GCATC 6 cut(s) 132, 214, 385, 391, 941, 982
SfcI CTRYAG 1 cut(s) 745
Sfr303I CCGCGG 1 cut(s) 264
SgrBI CCGCGG 1 cut(s) 264
SinI GGWCC 1 cut(s) 673
SmiMI CAYNNNNRTG 2 cut(s) 423, 990
SsiI CCGC 3 cut(s) 136, 261, 263
SspI AATATT 1 cut(s) 58
SspMI CTAG 2 cut(s) 515, 996
StyD4I CCNGG 1 cut(s) 805
TaaI ACNGT 5 cut(s) 464, 764, 823, 874, 970
TaiI ACGT 1 cut(s) 228
TaqI TCGA 1 cut(s) 173
TauI GCSGC 1 cut(s) 263
TfiI GAWTC 1 cut(s) 575
Tru1I TTAA 4 cut(s) 48, 222, 882, 921
Tru9I TTAA 4 cut(s) 48, 222, 882, 921
TscAI CASTG 1 cut(s) 973
TseFI GTSAC 4 cut(s) 326, 817, 829, 939
TseI GCWGC 2 cut(s) 511, 698
Tsp45I GTSAC 4 cut(s) 326, 817, 829, 939
TspRI CASTG 1 cut(s) 973
Van91I CCANNNNNTGG 1 cut(s) 32
VpaK11BI GGWCC 1 cut(s) 673
XagI CCTNNNNNAGG 1 cut(s) 668
XapI RAATTY 5 cut(s) 113, 193, 282, 923, 999
XcmI CCANNNNNNNNNTGG 1 cut(s) 29
XmnI GAANNNNTTC 1 cut(s) 1003
XspI CTAG 2 cut(s) 515, 996
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.