pycom16g22900

Belongs to the tetraspanin (TM4SF) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0024349
Physical Location & Seq
Forward (+)
23320231 .. 23322283
2053 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 798 bp
ATGAATCTAGCAAACAACCTGTTAGGCCTTCTCAACATCCTCACCTTCCTGATATCGGTTCCAATCGTCATTGTCGGTGTGCAGCTGTATAGGGTAAATGCGGCTGGACAGTGCTTGGAGGTTTTTAGGCTGCCTGTCCTCGTGTTGGGCGTGTTGATCATGGTGATGTCCATCGTAGGCATAATCAGTGCGTGTTGTCGTATTTCTTGGCTCCTCTGGATCTACCTCTTAGTAATGCTCCTCCTCCTCATCGGACTCATGAGCATTTATGCCATATCCTTCTTTATCATTTGGAAATCAGGCGGTGGAACTAGGGTTCCTGGTACAGAGCTGAAGGAGTACAAGCTCAGCGGTTACGCAGAGTGGTTGCAGAATACAGTTCAAGGAAAGGGTTGGGATAGCCTCAAGAACTGTCTCGTTGTTAGCCAAGCATGCAATTATATGCCCAAAAATGATACTAAAATCCAGGAGTTCAATATGAAGGAGTTGCCCCCCATTATGGCTGGTTGCTGTAAGCCATTAGCCGAGTGTGGATTTAGTTATAAGAGCCCCACCGTGTGGATAAGGAATAACAGCACTTCTTCCAGCCCTGACTGCAAAGCATGGAACAACGACCCAAAGGTTTTGTGCTACAACTGCGATTCTTGCAAGGCTGGAACTTTGGAACAACTACTGGATACCTGGAAGAGGATGCACTTTCTCAAAATTGTATTCCTTGGCTTCCTCGTGCTTGTCTATAGTATAGGATGCTGCGCATTTAAAAACACAAGGAAGAAACCTGACTATTGGAGACAATAA

Protein Analysis

266

Amino Acids

29.86

Weight (kDa)

9.03

Isoelectric Point (pI)

39.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tetraspanin PF00335 5 - 252 9.9e-24 Tetraspanin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0024349)

Species Orthologous Gene IDs
malus_domestica MD16G1261400.v1.1
prunus_persica Prupe.1G098300_v2.0.a1
pyrus_communis pycom16g22900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 543
Acc16I TGCGCA 1 cut(s) 754
AccB7I CCANNNNNTGG 1 cut(s) 558
AciI CCGC 3 cut(s) 101, 303, 351
AclWI GGATC 1 cut(s) 227
AcuI CTGAAG 1 cut(s) 353
AdeI CACNNNGTG 1 cut(s) 558
AfaI GTAC 2 cut(s) 325, 341
AfiI CCNNNNNNNGG 5 cut(s) 55, 145, 499, 558, 687
AgsI TTSAA 2 cut(s) 383, 475
AjnI CCWGG 3 cut(s) 319, 465, 680
AloI GAACNNNNNNTCC 4 cut(s) 300, 301, 332, 333
AluBI AGCT 3 cut(s) 85, 331, 346
AluI AGCT 3 cut(s) 85, 331, 346
Alw26I GTCTC 2 cut(s) 419, 784
AlwI GGATC 1 cut(s) 227
AoxI GGCC 1 cut(s) 25
ApeKI GCWGC 3 cut(s) 82, 130, 750
AspLEI GCGC 1 cut(s) 755
AsuHPI GGTGA 2 cut(s) 34, 175
BanII GRGCYC 1 cut(s) 551
BauI CACGAG 2 cut(s) 140, 725
BbvI GCAGC 3 cut(s) 94, 117, 737
BccI CCATC 1 cut(s) 179
BciT130I CCWGG 3 cut(s) 321, 467, 682
BciVI GTATCC 1 cut(s) 670
BclI TGATCA 1 cut(s) 156
BcoDI GTCTC 2 cut(s) 419, 784
BfaI CTAG 2 cut(s) 8, 312
BfmI CTRYAG 1 cut(s) 736
BfuI GTATCC 1 cut(s) 670
BisI GCNGC 4 cut(s) 83, 102, 131, 751
BlpI GCTNAGC 1 cut(s) 347
BlsI GCNGC 4 cut(s) 84, 103, 132, 752
Bme1390I CCNGG 3 cut(s) 321, 467, 682
BmiI GGNNCC 3 cut(s) 60, 212, 318
BmrFI CCNGG 3 cut(s) 321, 467, 682
BmsI GCATC 2 cut(s) 681, 737
Bpu1102I GCTNAGC 1 cut(s) 347
BpuEI CTTGAG 1 cut(s) 389
BsaBI GATNNNNATC 1 cut(s) 170
BsaJI CCNNGG 1 cut(s) 715
Bsc4I CCNNNNNNNGG 5 cut(s) 55, 145, 499, 558, 687
Bse1I ACTGG 1 cut(s) 678
Bse8I GATNNNNATC 1 cut(s) 170
BseBI CCWGG 3 cut(s) 321, 467, 682
BseDI CCNNGG 1 cut(s) 715
BseGI GGATG 3 cut(s) 36, 696, 752
BseJI GATNNNNATC 1 cut(s) 170
BseLI CCNNNNNNNGG 5 cut(s) 55, 145, 499, 558, 687
BseMII CTCAG 1 cut(s) 361
BseNI ACTGG 1 cut(s) 678
BseRI GAGGAG 4 cut(s) 203, 230, 233, 236
BseXI GCAGC 3 cut(s) 94, 117, 737
BsgI GTGCAG 1 cut(s) 101
BshFI GGCC 1 cut(s) 27
BslI CCNNNNNNNGG 5 cut(s) 55, 145, 499, 558, 687
BsmAI GTCTC 2 cut(s) 419, 784
BsnI GGCC 1 cut(s) 27
Bsp1286I GDGCHC 1 cut(s) 551
Bsp143I GATC 2 cut(s) 156, 219
Bsp1720I GCTNAGC 1 cut(s) 347
BspACI CCGC 3 cut(s) 101, 303, 351
BspANI GGCC 1 cut(s) 27
BspCNI CTCAG 1 cut(s) 360
BspHI TCATGA 1 cut(s) 258
BspLI GGNNCC 3 cut(s) 60, 212, 318
BspPI GGATC 1 cut(s) 227
BsrI ACTGG 1 cut(s) 678
BssECI CCNNGG 1 cut(s) 715
BssMI GATC 2 cut(s) 156, 219
BssSI CACGAG 2 cut(s) 140, 725
BssT1I CCWWGG 1 cut(s) 715
Bst2BI CACGAG 2 cut(s) 140, 725
Bst2UI CCWGG 3 cut(s) 321, 467, 682
Bst4CI ACNGT 4 cut(s) 111, 379, 413, 556
Bst6I CTCTTC 1 cut(s) 680
BstC8I GCNNGC 1 cut(s) 433
BstDEI CTNAG 2 cut(s) 229, 347
BstENI CCTNNNNNAGG 1 cut(s) 685
BstF5I GGATG 3 cut(s) 36, 696, 752
BstHHI GCGC 1 cut(s) 755
BstKTI GATC 2 cut(s) 159, 222
BstMAI GTCTC 2 cut(s) 419, 784
BstMBI GATC 2 cut(s) 156, 219
BstMWI GCNNNNNNNGC 4 cut(s) 432, 594, 636, 645
BstNI CCWGG 3 cut(s) 321, 467, 682
BstNSI RCATGY 1 cut(s) 435
BstSCI CCNGG 3 cut(s) 319, 465, 680
BstSFI CTRYAG 1 cut(s) 736
BstV1I GCAGC 3 cut(s) 94, 117, 737
BstX2I RGATCY 1 cut(s) 219
BstYI RGATCY 1 cut(s) 219
BsuI GTATCC 1 cut(s) 670
BsuRI GGCC 1 cut(s) 27
BtsCI GGATG 3 cut(s) 36, 696, 752
BtsIMutI CAGTG 2 cut(s) 116, 193
Cac8I GCNNGC 1 cut(s) 433
CciI TCATGA 1 cut(s) 258
CfoI GCGC 1 cut(s) 755
Csp6I GTAC 2 cut(s) 324, 340
CviAII CATG 4 cut(s) 160, 259, 432, 603
CviQI GTAC 2 cut(s) 324, 340
DdeI CTNAG 2 cut(s) 229, 347
DpnI GATC 2 cut(s) 158, 221
DpnII GATC 2 cut(s) 156, 219
DraI TTTAAA 1 cut(s) 760
DraIII CACNNNGTG 1 cut(s) 558
Eam1104I CTCTTC 1 cut(s) 680
EarI CTCTTC 1 cut(s) 680
Eco130I CCWWGG 1 cut(s) 715
Eco147I AGGCCT 1 cut(s) 27
Eco24I GRGCYC 1 cut(s) 551
Eco32I GATATC 1 cut(s) 54
Eco57I CTGAAG 1 cut(s) 353
EcoNI CCTNNNNNAGG 1 cut(s) 685
EcoRII CCWGG 3 cut(s) 319, 465, 680
EcoRV GATATC 1 cut(s) 54
EcoT14I CCWWGG 1 cut(s) 715
EcoT38I GRGCYC 1 cut(s) 551
ErhI CCWWGG 1 cut(s) 715
FaeI CATG 4 cut(s) 163, 262, 435, 606
FatI CATG 4 cut(s) 159, 258, 431, 602
FbaI TGATCA 1 cut(s) 156
Fnu4HI GCNGC 4 cut(s) 83, 102, 131, 751
FokI GGATG 3 cut(s) 23, 703, 759
FriOI GRGCYC 1 cut(s) 551
Fsp4HI GCNGC 4 cut(s) 83, 102, 131, 751
FspBI CTAG 2 cut(s) 8, 312
FspI TGCGCA 1 cut(s) 754
GlaI GCGC 1 cut(s) 754
GluI GCNGC 4 cut(s) 83, 102, 131, 751
HaeIII GGCC 1 cut(s) 27
HhaI GCGC 1 cut(s) 755
Hin1II CATG 4 cut(s) 163, 262, 435, 606
Hin6I GCGC 1 cut(s) 753
HinP1I GCGC 1 cut(s) 753
HinfI GANTC 3 cut(s) 4, 255, 641
HphI GGTGA 2 cut(s) 34, 175
Hpy188I TCNGA 1 cut(s) 254
Hpy188III TCNNGA 4 cut(s) 49, 217, 259, 406
HpyAV CCTTC 5 cut(s) 38, 55, 289, 328, 475
HpyCH4III ACNGT 4 cut(s) 111, 379, 413, 556
HpyCH4V TGCA 6 cut(s) 82, 370, 435, 597, 648, 694
HpyF10VI GCNNNNNNNGC 4 cut(s) 432, 594, 636, 645
HpyF3I CTNAG 2 cut(s) 229, 347
Hsp92II CATG 4 cut(s) 163, 262, 435, 606
HspAI GCGC 1 cut(s) 753
Ksp22I TGATCA 1 cut(s) 156
Kzo9I GATC 2 cut(s) 156, 219
LmnI GCTCC 2 cut(s) 216, 243
Lsp1109I GCAGC 3 cut(s) 94, 117, 737
LweI GCATC 2 cut(s) 681, 737
MaeI CTAG 2 cut(s) 8, 312
MaeIII GTNAC 1 cut(s) 353
MalI GATC 2 cut(s) 158, 221
MboI GATC 2 cut(s) 156, 219
MboII GAAGA 3 cut(s) 573, 697, 784
MflI RGATCY 1 cut(s) 219
MhlI GDGCHC 1 cut(s) 551
MluCI AATT 2 cut(s) 436, 705
MlyI GAGTC 1 cut(s) 249
MseI TTAA 1 cut(s) 759
MslI CAYNNNNRTG 1 cut(s) 164
MspA1I CMGCKG 2 cut(s) 85, 351
MspR9I CCNGG 3 cut(s) 321, 467, 682
MvaI CCWGG 3 cut(s) 321, 467, 682
MwoI GCNNNNNNNGC 4 cut(s) 432, 594, 636, 645
NdeII GATC 2 cut(s) 156, 219
NlaIII CATG 4 cut(s) 163, 262, 435, 606
NlaIV GGNNCC 3 cut(s) 60, 212, 318
NmeAIII GCCGAG 1 cut(s) 550
NsbI TGCGCA 1 cut(s) 754
NspI RCATGY 1 cut(s) 435
PaeI GCATGC 1 cut(s) 435
PagI TCATGA 1 cut(s) 258
PceI AGGCCT 1 cut(s) 27
PcsI WCGNNNNNNNCGW 1 cut(s) 147
PfeI GAWTC 2 cut(s) 4, 641
PflMI CCANNNNNTGG 1 cut(s) 558
PfoI TCCNGGA 1 cut(s) 465
PkrI GCNGC 4 cut(s) 84, 103, 132, 752
PleI GAGTC 1 cut(s) 249
PpsI GAGTC 1 cut(s) 249
PsiI TTATAA 1 cut(s) 543
Psp6I CCWGG 3 cut(s) 319, 465, 680
PspGI CCWGG 3 cut(s) 319, 465, 680
PspN4I GGNNCC 3 cut(s) 60, 212, 318
PsuI RGATCY 1 cut(s) 219
PvuII CAGCTG 1 cut(s) 85
RsaI GTAC 2 cut(s) 325, 341
RsaNI GTAC 2 cut(s) 324, 340
RseI CAYNNNNRTG 1 cut(s) 164
SaqAI TTAA 1 cut(s) 759
SatI GCNGC 4 cut(s) 83, 102, 131, 751
Sau3AI GATC 2 cut(s) 156, 219
SchI GAGTC 1 cut(s) 249
ScrFI CCNGG 3 cut(s) 321, 467, 682
SduI GDGCHC 1 cut(s) 551
SfaNI GCATC 2 cut(s) 681, 737
SfcI CTRYAG 1 cut(s) 736
SmiMI CAYNNNNRTG 1 cut(s) 164
SmlI CTYRAG 1 cut(s) 404
SmoI CTYRAG 1 cut(s) 404
SphI GCATGC 1 cut(s) 435
Sse9I AATT 2 cut(s) 436, 705
SseBI AGGCCT 1 cut(s) 27
SsiI CCGC 3 cut(s) 101, 303, 351
SspMI CTAG 2 cut(s) 8, 312
StuI AGGCCT 1 cut(s) 27
StyD4I CCNGG 3 cut(s) 319, 465, 680
StyI CCWWGG 1 cut(s) 715
TaaI ACNGT 4 cut(s) 111, 379, 413, 556
TasI AATT 2 cut(s) 436, 705
TatI WGTACW 1 cut(s) 339
TauI GCSGC 1 cut(s) 104
TfiI GAWTC 2 cut(s) 4, 641
Tru1I TTAA 1 cut(s) 759
Tru9I TTAA 1 cut(s) 759
TscAI CASTG 2 cut(s) 116, 193
TseI GCWGC 3 cut(s) 82, 130, 750
TspDTI ATGAA 2 cut(s) 17, 494
TspRI CASTG 2 cut(s) 116, 193
Van91I CCANNNNNTGG 1 cut(s) 558
XagI CCTNNNNNAGG 1 cut(s) 685
XceI RCATGY 1 cut(s) 435
XspI CTAG 2 cut(s) 8, 312
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.