pycom16g23750

Regulator of nonsense transcripts 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
N/A
Physical Location & Seq
Forward (+)
25012949 .. 25015199
2251 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 672 bp
ATGAAAGCTTTTGCAGTGGACGAGACAAGTGTTAGTGGATATATTTATCATCATTTGTTAGGCCATGAAGTAGAGGTTCAAATGGTCCGTAATTCATTGCCTCGTCGTTTTGGTGCACCTGGTCTTCCGGAGCTCAATGCATCTCAAGTTTTTGCTGTAAAGAGTGTCCTTCAGAAGCCTATAAGTTTGATCCAGGGTCCACCTGGCACAGGAAAAACTGTGACTTCTGCAGCAATTGTGTATCACATGGCTAAACAAGGCCAGGGACAGGTTTTGGTGTGTGCTCCTAGTAATGTGGCTGTAGACCAACTAGCTGAGAAGATAAGTGCAACTGGGTTGAAGGTTGTTAGACTTTGTGCAAAATCAAGGGAAGCTGTAAGTTCTCCTGTTGAGCATTTAACCCTTCATTACCAGGTTCGTCATCTTGACATGTCAGAAAAGAGTGAACTTCACAAGCTGCAACAATTGAAGGATGAGCAAGGAGAATTGTCTAGTAATGATGAGAAAAAATATAAGGCACTGAAGCGGGCAACCGAGAGGGAGATTTCTCAAAGTGCTGATGTCATTTGTTGCACATGTGTTGGTGCTGGATCGATCCTCGATTGGCCAATTTTAGGTTCCGTCAGGTACTTATTGATGAGTCGACTCAGGCTACAGAACCTGAATGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000184 GO:0000932 GO:0000956 GO:0001101 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0006139 GO:0006401 GO:0006402 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009314 GO:0009416 GO:0009506 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009628 GO:0009648 GO:0009719 GO:0009725 GO:0009743 GO:0009751 GO:0009753 GO:0009755 GO:0009756 GO:0009863 GO:0009867 GO:0009892 GO:0009987 GO:0010033 GO:0010182 GO:0010468 GO:0010605 GO:0010629 GO:0014070 GO:0016020 GO:0016070 GO:0016071 GO:0019222 GO:0019439 GO:0023052 GO:0030054 GO:0032870 GO:0032991 GO:0034641 GO:0034655 GO:0035690 GO:0035770 GO:0036464 GO:0042221 GO:0042493 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046677 GO:0046700 GO:0048519 GO:0048571 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055044 GO:0060255 GO:0065007 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071322 GO:0071395 GO:0071407 GO:0071446 GO:0071495 GO:0071704 GO:0071944 GO:0090304 GO:0097159 GO:0098542 GO:1901360 GO:1901361 GO:1901363 GO:1901575 GO:1901700 GO:1901701 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 map03013 map03015
Pfam Domains
Protein Families
AAA_11 AAA_12 ResIII UPF1_Zn_bind

Protein Analysis

224

Amino Acids

24.43

Weight (kDa)

8.94

Isoelectric Point (pI)

43.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_11 PF13086 44 - 166 9.9e-25 AAA domain
AAA_30 PF13604 44 - 131 7.6e-13 AAA domain
ResIII PF04851 44 - 115 2.5e-07 Type III restriction enzyme, res subunit
AAA_19 PF13245 51 - 137 2.6e-10 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 303, 643
AccIII TCCGGA 1 cut(s) 127
AciI CCGC 1 cut(s) 526
AclWI GGATC 3 cut(s) 184, 589, 598
AcoI YGGCCR 1 cut(s) 605
AcuI CTGAAG 2 cut(s) 155, 542
AfaI GTAC 1 cut(s) 629
AfiI CCNNNNNNNGG 3 cut(s) 209, 268, 614
AflIII ACRYGT 2 cut(s) 429, 575
AgsI TTSAA 3 cut(s) 80, 340, 469
AjnI CCWGG 5 cut(s) 118, 192, 202, 261, 411
AjuI GAANNNNNNNTTGG 2 cut(s) 601, 633
AluBI AGCT 5 cut(s) 8, 133, 314, 374, 457
AluI AGCT 5 cut(s) 8, 133, 314, 374, 457
Alw21I GWGCWC 3 cut(s) 118, 135, 286
Alw26I GTCTC 1 cut(s) 17
Alw44I GTGCAC 1 cut(s) 114
AlwI GGATC 3 cut(s) 184, 589, 598
AlwNI CAGNNNCTG 1 cut(s) 661
Aor13HI TCCGGA 1 cut(s) 127
AoxI GGCC 3 cut(s) 61, 259, 605
ApaLI GTGCAC 1 cut(s) 114
ApeKI GCWGC 2 cut(s) 230, 457
AspS9I GGNCC 2 cut(s) 85, 197
AvaII GGWCC 2 cut(s) 85, 197
BaeGI GKGCMC 1 cut(s) 118
BalI TGGCCA 1 cut(s) 607
BanII GRGCYC 1 cut(s) 135
BbsI GAAGAC 1 cut(s) 116
Bbv12I GWGCWC 3 cut(s) 118, 135, 286
BbvI GCAGC 2 cut(s) 242, 444
BciT130I CCWGG 5 cut(s) 120, 194, 204, 263, 413
BcoDI GTCTC 1 cut(s) 17
BfaI CTAG 3 cut(s) 288, 311, 492
BfmI CTRYAG 3 cut(s) 228, 300, 653
BisI GCNGC 2 cut(s) 231, 458
BlsI GCNGC 2 cut(s) 232, 459
Bme1390I CCNGG 5 cut(s) 120, 194, 204, 263, 413
Bme18I GGWCC 2 cut(s) 85, 197
BmgT120I GGNCC 2 cut(s) 85, 197
BmiI GGNNCC 2 cut(s) 198, 619
BmrFI CCNGG 5 cut(s) 120, 194, 204, 263, 413
BmrI ACTGGG 1 cut(s) 342
BmsI GCATC 1 cut(s) 149
BmuI ACTGGG 1 cut(s) 342
BpiI GAAGAC 1 cut(s) 116
BpuEI CTTGAG 1 cut(s) 129
Bsa29I ATCGAT 1 cut(s) 593
BsaJI CCNNGG 2 cut(s) 193, 262
BsaWI WCCGGW 1 cut(s) 127
Bsc4I CCNNNNNNNGG 3 cut(s) 209, 268, 614
Bse1I ACTGG 1 cut(s) 337
Bse3DI GCAATG 1 cut(s) 95
BseAI TCCGGA 1 cut(s) 127
BseBI CCWGG 5 cut(s) 120, 194, 204, 263, 413
BseCI ATCGAT 1 cut(s) 593
BseDI CCNNGG 2 cut(s) 193, 262
BseGI GGATG 1 cut(s) 478
BseLI CCNNNNNNNGG 3 cut(s) 209, 268, 614
BseMI GCAATG 1 cut(s) 95
BseMII CTCAG 2 cut(s) 306, 661
BseNI ACTGG 1 cut(s) 337
BseSI GKGCMC 1 cut(s) 118
BseXI GCAGC 2 cut(s) 242, 444
BshFI GGCC 3 cut(s) 63, 261, 607
BshVI ATCGAT 1 cut(s) 593
BsiHKAI GWGCWC 3 cut(s) 118, 135, 286
BsiSI CCGG 1 cut(s) 128
BslFI GGGAC 1 cut(s) 279
BslI CCNNNNNNNGG 3 cut(s) 209, 268, 614
BsmAI GTCTC 1 cut(s) 17
BsmFI GGGAC 1 cut(s) 279
BsmI GAATGC 1 cut(s) 670
BsnI GGCC 3 cut(s) 63, 261, 607
Bsp1286I GDGCHC 3 cut(s) 118, 135, 286
Bsp13I TCCGGA 1 cut(s) 127
Bsp143I GATC 3 cut(s) 189, 590, 594
BspACI CCGC 1 cut(s) 526
BspANI GGCC 3 cut(s) 63, 261, 607
BspCNI CTCAG 2 cut(s) 307, 660
BspDI ATCGAT 1 cut(s) 593
BspEI TCCGGA 1 cut(s) 127
BspLI GGNNCC 2 cut(s) 198, 619
BspMAI CTGCAG 1 cut(s) 232
BspPI GGATC 3 cut(s) 184, 589, 598
BsrDI GCAATG 1 cut(s) 95
BsrI ACTGG 1 cut(s) 337
BssECI CCNNGG 2 cut(s) 193, 262
BssMI GATC 3 cut(s) 189, 590, 594
Bst2UI CCWGG 5 cut(s) 120, 194, 204, 263, 413
Bst4CI ACNGT 1 cut(s) 220
BstC8I GCNNGC 1 cut(s) 528
BstDEI CTNAG 2 cut(s) 315, 647
BstENI CCTNNNNNAGG 1 cut(s) 207
BstF5I GGATG 1 cut(s) 478
BstKTI GATC 3 cut(s) 192, 593, 597
BstMAI GTCTC 1 cut(s) 17
BstMBI GATC 3 cut(s) 189, 590, 594
BstNI CCWGG 5 cut(s) 120, 194, 204, 263, 413
BstNSI RCATGY 2 cut(s) 433, 579
BstSCI CCNGG 5 cut(s) 118, 192, 202, 261, 411
BstSFI CTRYAG 3 cut(s) 228, 300, 653
BstSLI GKGCMC 1 cut(s) 118
BstV1I GCAGC 2 cut(s) 242, 444
BstV2I GAAGAC 1 cut(s) 116
Bsu15I ATCGAT 1 cut(s) 593
BsuRI GGCC 3 cut(s) 63, 261, 607
BsuTUI ATCGAT 1 cut(s) 593
BtsCI GGATG 1 cut(s) 478
BtsI GCAGTG 1 cut(s) 21
BtsIMutI CAGTG 2 cut(s) 21, 518
Cac8I GCNNGC 1 cut(s) 528
CaiI CAGNNNCTG 1 cut(s) 661
Cfr13I GGNCC 2 cut(s) 85, 197
ClaI ATCGAT 1 cut(s) 593
CsiI ACCWGGT 2 cut(s) 118, 411
Csp6I GTAC 1 cut(s) 628
CviAII CATG 4 cut(s) 65, 247, 430, 576
CviQI GTAC 1 cut(s) 628
DdeI CTNAG 2 cut(s) 315, 647
DpnI GATC 3 cut(s) 191, 592, 596
DpnII GATC 3 cut(s) 189, 590, 594
EaeI YGGCCR 1 cut(s) 605
Ecl136II GAGCTC 1 cut(s) 133
Eco24I GRGCYC 1 cut(s) 135
Eco47I GGWCC 2 cut(s) 85, 197
Eco53kI GAGCTC 1 cut(s) 133
Eco57I CTGAAG 2 cut(s) 155, 542
EcoICRI GAGCTC 1 cut(s) 133
EcoNI CCTNNNNNAGG 1 cut(s) 207
EcoRII CCWGG 5 cut(s) 118, 192, 202, 261, 411
EcoT22I ATGCAT 1 cut(s) 142
EcoT38I GRGCYC 1 cut(s) 135
FaeI CATG 4 cut(s) 68, 250, 433, 579
FaiI YATR 7 cut(s) 42, 66, 182, 248, 431, 513, 577
FaqI GGGAC 1 cut(s) 279
FatI CATG 4 cut(s) 64, 246, 429, 575
FauI CCCGC 1 cut(s) 519
FblI GTMKAC 2 cut(s) 303, 643
Fnu4HI GCNGC 2 cut(s) 231, 458
FokI GGATG 1 cut(s) 485
FriOI GRGCYC 1 cut(s) 135
Fsp4HI GCNGC 2 cut(s) 231, 458
FspBI CTAG 3 cut(s) 288, 311, 492
GluI GCNGC 2 cut(s) 231, 458
HaeIII GGCC 3 cut(s) 63, 261, 607
HapII CCGG 1 cut(s) 128
Hin1II CATG 4 cut(s) 68, 250, 433, 579
HincII GTYRAC 1 cut(s) 644
HindII GTYRAC 1 cut(s) 644
HindIII AAGCTT 1 cut(s) 6
HinfI GANTC 2 cut(s) 640, 645
HpaII CCGG 1 cut(s) 128
Hpy166II GTNNAC 6 cut(s) 19, 116, 200, 304, 446, 644
Hpy188I TCNGA 2 cut(s) 174, 436
Hpy188III TCNNGA 2 cut(s) 128, 425
Hpy8I GTNNAC 6 cut(s) 19, 116, 200, 304, 446, 644
Hpy99I CGWCG 1 cut(s) 108
HpyAV CCTTC 4 cut(s) 179, 334, 413, 463
HpyCH4III ACNGT 1 cut(s) 220
HpyCH4V TGCA 8 cut(s) 14, 116, 140, 230, 329, 359, 460, 573
HpyF3I CTNAG 2 cut(s) 315, 647
Hsp92II CATG 4 cut(s) 68, 250, 433, 579
Kpn2I TCCGGA 1 cut(s) 127
Kzo9I GATC 3 cut(s) 189, 590, 594
LmnI GCTCC 2 cut(s) 130, 289
Lsp1109I GCAGC 2 cut(s) 242, 444
LweI GCATC 1 cut(s) 149
MabI ACCWGGT 2 cut(s) 118, 411
MaeI CTAG 3 cut(s) 288, 311, 492
MaeIII GTNAC 1 cut(s) 220
MalI GATC 3 cut(s) 191, 592, 596
MboI GATC 3 cut(s) 189, 590, 594
MboII GAAGA 2 cut(s) 116, 331
MfeI CAATTG 2 cut(s) 234, 464
MhlI GDGCHC 3 cut(s) 118, 135, 286
MlsI TGGCCA 1 cut(s) 607
MluCI AATT 5 cut(s) 91, 234, 464, 485, 609
MluNI TGGCCA 1 cut(s) 607
MlyI GAGTC 2 cut(s) 639, 649
MnlI CCTC 4 cut(s) 67, 111, 531, 608
Mox20I TGGCCA 1 cut(s) 607
Mph1103I ATGCAT 1 cut(s) 142
MroI TCCGGA 1 cut(s) 127
MscI TGGCCA 1 cut(s) 607
MseI TTAA 1 cut(s) 398
Msp20I TGGCCA 1 cut(s) 607
MspI CCGG 1 cut(s) 128
MspR9I CCNGG 5 cut(s) 120, 194, 204, 263, 413
MunI CAATTG 2 cut(s) 234, 464
Mva1269I GAATGC 1 cut(s) 670
MvaI CCWGG 5 cut(s) 120, 194, 204, 263, 413
NdeII GATC 3 cut(s) 189, 590, 594
NlaIII CATG 4 cut(s) 68, 250, 433, 579
NlaIV GGNNCC 2 cut(s) 198, 619
NmuCI GTSAC 1 cut(s) 220
NsiI ATGCAT 1 cut(s) 142
NspI RCATGY 2 cut(s) 433, 579
PciI ACATGT 2 cut(s) 429, 575
PctI GAATGC 1 cut(s) 670
PkrI GCNGC 2 cut(s) 232, 459
PleI GAGTC 2 cut(s) 639, 648
PpsI GAGTC 2 cut(s) 639, 648
PscI ACATGT 2 cut(s) 429, 575
Psp124BI GAGCTC 1 cut(s) 135
Psp6I CCWGG 5 cut(s) 118, 192, 202, 261, 411
PspGI CCWGG 5 cut(s) 118, 192, 202, 261, 411
PspN4I GGNNCC 2 cut(s) 198, 619
PspPI GGNCC 2 cut(s) 85, 197
PstI CTGCAG 1 cut(s) 232
PstNI CAGNNNCTG 1 cut(s) 661
RsaI GTAC 1 cut(s) 629
RsaNI GTAC 1 cut(s) 628
SacI GAGCTC 1 cut(s) 135
SalI GTCGAC 1 cut(s) 642
SaqAI TTAA 1 cut(s) 398
SatI GCNGC 2 cut(s) 231, 458
Sau3AI GATC 3 cut(s) 189, 590, 594
Sau96I GGNCC 2 cut(s) 85, 197
SchI GAGTC 2 cut(s) 639, 649
ScrFI CCNGG 5 cut(s) 120, 194, 204, 263, 413
SduI GDGCHC 3 cut(s) 118, 135, 286
SexAI ACCWGGT 2 cut(s) 118, 411
SfaNI GCATC 1 cut(s) 149
SfcI CTRYAG 3 cut(s) 228, 300, 653
SinI GGWCC 2 cut(s) 85, 197
SmlI CTYRAG 1 cut(s) 144
SmoI CTYRAG 1 cut(s) 144
Sse9I AATT 5 cut(s) 91, 234, 464, 485, 609
SsiI CCGC 1 cut(s) 526
SspMI CTAG 3 cut(s) 288, 311, 492
SstI GAGCTC 1 cut(s) 135
StyD4I CCNGG 5 cut(s) 118, 192, 202, 261, 411
TaaI ACNGT 1 cut(s) 220
TaqI TCGA 3 cut(s) 593, 600, 643
TasI AATT 5 cut(s) 91, 234, 464, 485, 609
Tru1I TTAA 1 cut(s) 398
Tru9I TTAA 1 cut(s) 398
TscAI CASTG 2 cut(s) 21, 525
TseFI GTSAC 1 cut(s) 220
TseI GCWGC 2 cut(s) 230, 457
Tsp45I GTSAC 1 cut(s) 220
TspDTI ATGAA 4 cut(s) 17, 81, 84, 395
TspGWI ACGGA 2 cut(s) 77, 610
TspRI CASTG 2 cut(s) 21, 525
VneI GTGCAC 1 cut(s) 114
VpaK11BI GGWCC 2 cut(s) 85, 197
XagI CCTNNNNNAGG 1 cut(s) 207
XceI RCATGY 2 cut(s) 433, 579
XcmI CCANNNNNNNNNTGG 1 cut(s) 200
XmiI GTMKAC 2 cut(s) 303, 643
XspI CTAG 3 cut(s) 288, 311, 492
Zsp2I ATGCAT 1 cut(s) 142
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.