pycom16g24190

Belongs to the 3-beta-HSD family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
N/A
Physical Location & Seq
Forward (+)
25450932 .. 25451358
427 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 318 bp
ATGGCACCAGCAGCAGCATCGGACTCTTCGAACAGAGCTGTATGTGTAGTGGATGCATCAGGCCACTTAGGCAGCAGCCTCGCTAAGCGCCTGCTGCAAAAGGGTTACACGGTCCATGCTGCCCTTCAGAAACATGGGGAATCGCGGTTGGGTGGGACTTCCTTTGACAAAAAGAAGCTCAAAGTTTTCAATCTGGATCCGTTCGACTACCGGAGCATTCTTGACGCTCTCAAAGGCCGTTCTGGCTTGTTTTATTCATTTGAGCCTCCACAAGATCAACCAGACTATGATGTACGTACCTTCTTTCTTCCTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
3Beta_HSD Epimerase NAD_binding_10

Protein Analysis

106

Amino Acids

11.53

Weight (kDa)

9.13

Isoelectric Point (pI)

52.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 4
AccII CGCG 1 cut(s) 145
AciI CCGC 1 cut(s) 145
AclWI GGATC 2 cut(s) 191, 204
AcuI CTGAAG 1 cut(s) 110
AfaI GTAC 2 cut(s) 294, 298
AgsI TTSAA 1 cut(s) 190
AluBI AGCT 2 cut(s) 38, 178
AluI AGCT 2 cut(s) 38, 178
AlwI GGATC 2 cut(s) 191, 204
AoxI GGCC 2 cut(s) 61, 235
ApeKI GCWGC 6 cut(s) 11, 14, 72, 75, 94, 119
AspLEI GCGC 1 cut(s) 90
AspS9I GGNCC 1 cut(s) 112
AsuII TTCGAA 1 cut(s) 29
AvaII GGWCC 1 cut(s) 112
BamHI GGATCC 1 cut(s) 196
BanI GGYRCC 1 cut(s) 4
BbvI GCAGC 6 cut(s) 23, 26, 81, 84, 87, 106
BceAI ACGGC 1 cut(s) 222
BcgI CGANNNNNNTGC 3 cut(s) 34, 61, 95
BfoI RGCGCY 1 cut(s) 91
BglI GCCNNNNNGGC 2 cut(s) 69, 243
BisI GCNGC 6 cut(s) 12, 15, 73, 76, 95, 120
BlpI GCTNAGC 1 cut(s) 84
BlsI GCNGC 6 cut(s) 13, 16, 74, 77, 96, 121
Bme18I GGWCC 1 cut(s) 112
BmgT120I GGNCC 1 cut(s) 112
BmiI GGNNCC 2 cut(s) 6, 198
BmsI GCATC 3 cut(s) 26, 43, 65
Bpu1102I GCTNAGC 1 cut(s) 84
Bpu14I TTCGAA 1 cut(s) 29
BsaAI YACGTR 1 cut(s) 296
BsaWI WCCGGW 1 cut(s) 210
BseGI GGATG 1 cut(s) 58
BseXI GCAGC 6 cut(s) 23, 26, 81, 84, 87, 106
Bsh1236I CGCG 1 cut(s) 145
BshFI GGCC 2 cut(s) 63, 237
BshNI GGYRCC 1 cut(s) 4
BsiSI CCGG 1 cut(s) 211
BslFI GGGAC 1 cut(s) 169
BsmFI GGGAC 1 cut(s) 169
BsmI GAATGC 1 cut(s) 216
BsnI GGCC 2 cut(s) 63, 237
Bsp119I TTCGAA 1 cut(s) 29
Bsp143I GATC 2 cut(s) 196, 274
Bsp1720I GCTNAGC 1 cut(s) 84
BspACI CCGC 1 cut(s) 145
BspANI GGCC 2 cut(s) 63, 237
BspFNI CGCG 1 cut(s) 145
BspLI GGNNCC 2 cut(s) 6, 198
BspPI GGATC 2 cut(s) 191, 204
BspT104I TTCGAA 1 cut(s) 29
BspT107I GGYRCC 1 cut(s) 4
BssMI GATC 2 cut(s) 196, 274
Bst4CI ACNGT 1 cut(s) 112
Bst6I CTCTTC 1 cut(s) 31
BstBAI YACGTR 1 cut(s) 296
BstBI TTCGAA 1 cut(s) 29
BstC8I GCNNGC 1 cut(s) 92
BstDEI CTNAG 2 cut(s) 67, 84
BstF5I GGATG 1 cut(s) 58
BstFNI CGCG 1 cut(s) 145
BstH2I RGCGCY 1 cut(s) 91
BstHHI GCGC 1 cut(s) 90
BstKTI GATC 2 cut(s) 199, 277
BstMBI GATC 2 cut(s) 196, 274
BstMWI GCNNNNNNNGC 4 cut(s) 11, 69, 94, 243
BstSNI TACGTA 1 cut(s) 296
BstUI CGCG 1 cut(s) 145
BstV1I GCAGC 6 cut(s) 23, 26, 81, 84, 87, 106
BstX2I RGATCY 1 cut(s) 196
BstYI RGATCY 1 cut(s) 196
BsuRI GGCC 2 cut(s) 63, 237
BtsCI GGATG 1 cut(s) 58
Cac8I GCNNGC 1 cut(s) 92
CfoI GCGC 1 cut(s) 90
Cfr13I GGNCC 1 cut(s) 112
CseI GACGC 1 cut(s) 233
Csp6I GTAC 2 cut(s) 293, 297
CviAII CATG 2 cut(s) 116, 134
CviJI RGCY 7 cut(s) 38, 63, 78, 178, 237, 246, 265
CviKI_1 RGCY 7 cut(s) 38, 63, 78, 178, 237, 246, 265
CviQI GTAC 2 cut(s) 293, 297
DdeI CTNAG 2 cut(s) 67, 84
DpnI GATC 2 cut(s) 198, 276
DpnII GATC 2 cut(s) 196, 274
Eam1104I CTCTTC 1 cut(s) 31
EarI CTCTTC 1 cut(s) 31
Eco105I TACGTA 1 cut(s) 296
Eco47I GGWCC 1 cut(s) 112
Eco57I CTGAAG 1 cut(s) 110
EcoT22I ATGCAT 1 cut(s) 58
FaeI CATG 2 cut(s) 119, 137
FaiI YATR 4 cut(s) 43, 117, 135, 288
FaqI GGGAC 1 cut(s) 169
FatI CATG 2 cut(s) 115, 133
Fnu4HI GCNGC 6 cut(s) 12, 15, 73, 76, 95, 120
FokI GGATG 1 cut(s) 65
Fsp4HI GCNGC 6 cut(s) 12, 15, 73, 76, 95, 120
GlaI GCGC 1 cut(s) 89
GluI GCNGC 6 cut(s) 12, 15, 73, 76, 95, 120
HaeII RGCGCY 1 cut(s) 91
HaeIII GGCC 2 cut(s) 63, 237
HapII CCGG 1 cut(s) 211
HgaI GACGC 1 cut(s) 233
HhaI GCGC 1 cut(s) 90
Hin1II CATG 2 cut(s) 119, 137
Hin6I GCGC 1 cut(s) 88
HinP1I GCGC 1 cut(s) 88
HinfI GANTC 2 cut(s) 23, 140
HpaII CCGG 1 cut(s) 211
Hpy188I TCNGA 2 cut(s) 22, 129
Hpy188III TCNNGA 2 cut(s) 194, 221
HpyAV CCTTC 2 cut(s) 134, 310
HpyCH4III ACNGT 1 cut(s) 112
HpyCH4IV ACGT 1 cut(s) 295
HpyCH4V TGCA 2 cut(s) 56, 97
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 69, 94, 243
HpyF3I CTNAG 2 cut(s) 67, 84
HpySE526I ACGT 1 cut(s) 295
Hsp92II CATG 2 cut(s) 119, 137
HspAI GCGC 1 cut(s) 88
Kzo9I GATC 2 cut(s) 196, 274
LmnI GCTCC 1 cut(s) 213
LpnPI CCDG 7 cut(s) 21, 45, 104, 179, 224, 228, 294
Lsp1109I GCAGC 6 cut(s) 23, 26, 81, 84, 87, 106
LweI GCATC 3 cut(s) 26, 43, 65
MaeII ACGT 1 cut(s) 295
MaeIII GTNAC 1 cut(s) 104
MalI GATC 2 cut(s) 198, 276
MboI GATC 2 cut(s) 196, 274
MboII GAAGA 2 cut(s) 18, 299
MflI RGATCY 1 cut(s) 196
MluCI AATT 1 cut(s) 313
MlyI GAGTC 1 cut(s) 17
MnlI CCTC 2 cut(s) 89, 276
Mph1103I ATGCAT 1 cut(s) 58
MspI CCGG 1 cut(s) 211
Mva1269I GAATGC 1 cut(s) 216
MvnI CGCG 1 cut(s) 145
MwoI GCNNNNNNNGC 4 cut(s) 11, 69, 94, 243
NdeII GATC 2 cut(s) 196, 274
NlaIII CATG 2 cut(s) 119, 137
NlaIV GGNNCC 2 cut(s) 6, 198
NsiI ATGCAT 1 cut(s) 58
NspV TTCGAA 1 cut(s) 29
PcsI WCGNNNNNNNCGW 1 cut(s) 26
PctI GAATGC 1 cut(s) 216
PfeI GAWTC 1 cut(s) 140
PkrI GCNGC 6 cut(s) 13, 16, 74, 77, 96, 121
PleI GAGTC 1 cut(s) 17
PpsI GAGTC 1 cut(s) 17
Ppu21I YACGTR 1 cut(s) 296
PspN4I GGNNCC 2 cut(s) 6, 198
PspPI GGNCC 1 cut(s) 112
PsuI RGATCY 1 cut(s) 196
RsaI GTAC 2 cut(s) 294, 298
RsaNI GTAC 2 cut(s) 293, 297
SatI GCNGC 6 cut(s) 12, 15, 73, 76, 95, 120
Sau3AI GATC 2 cut(s) 196, 274
Sau96I GGNCC 1 cut(s) 112
SchI GAGTC 1 cut(s) 17
SetI ASST 4 cut(s) 40, 180, 298, 302
SfaNI GCATC 3 cut(s) 26, 43, 65
SfuI TTCGAA 1 cut(s) 29
SinI GGWCC 1 cut(s) 112
SnaBI TACGTA 1 cut(s) 296
Sse9I AATT 1 cut(s) 313
SsiI CCGC 1 cut(s) 145
TaaI ACNGT 1 cut(s) 112
TaiI ACGT 1 cut(s) 298
TaqI TCGA 2 cut(s) 29, 204
TasI AATT 1 cut(s) 313
TfiI GAWTC 1 cut(s) 140
TseI GCWGC 6 cut(s) 11, 14, 72, 75, 94, 119
TspDTI ATGAA 1 cut(s) 246
TspGWI ACGGA 1 cut(s) 189
VpaK11BI GGWCC 1 cut(s) 112
Zsp2I ATGCAT 1 cut(s) 58
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.