pycom16g26330

Protein of unknown function, DUF547

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
N/A
Physical Location & Seq
Forward (+)
28762666 .. 28764934
2269 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 399 bp
ATGAAGAGCGCTACTTCTGATAAGATTAATGGACTCTTCTCCTTGTTTAACCCTCTTATTGCACGGTTGGATAGGACTTCCCTTGATCAGGCTTCCCTTTGCTGGGATTCTTACAAGGCACTGGGTTCTAGTTCCGTCAACTCTATTGCTTCCCTTGATCGCGCCTTTCAGCTTCTCCTACTTCCTCCTTGTCCTCCTCAAGTATATTTTCTTCTAAGAATCCTTGTGATAGTTGGAGTCCACATTGCAATGAATATGCTACAGTGCATCAAGGGAAAGAATTTGAAGGCGGGGAGCACTAGACATTTATACGCATTAGAATACCCTGAGCCACTAGTCCATTTTGCTCTATGTTCAGGGGCATGCTCTGACCCAGCGGTAACAAGTTTCTTCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF547 Lzipper-MIP1

Protein Analysis

133

Amino Acids

14.48

Weight (kDa)

8.67

Isoelectric Point (pI)

31.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF547 PF04784 83 - 129 2.7e-07 Protein of unknown function, DUF547
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 162
AciI CCGC 2 cut(s) 290, 377
AcsI RAATTY 1 cut(s) 280
AfeI AGCGCT 1 cut(s) 10
AfiI CCNNNNNNNGG 3 cut(s) 88, 102, 103
AgsI TTSAA 1 cut(s) 286
AhlI ACTAGT 1 cut(s) 334
AluBI AGCT 1 cut(s) 172
AluI AGCT 1 cut(s) 172
Alw21I GWGCWC 1 cut(s) 299
Aor51HI AGCGCT 1 cut(s) 10
ApoI RAATTY 1 cut(s) 280
AseI ATTAAT 1 cut(s) 27
AspLEI GCGC 2 cut(s) 11, 164
BarI GAAGNNNNNNTAC 2 cut(s) 195, 227
Bbv12I GWGCWC 1 cut(s) 299
BclI TGATCA 1 cut(s) 85
BcuI ACTAGT 1 cut(s) 334
BfaI CTAG 3 cut(s) 129, 300, 335
BfmI CTRYAG 1 cut(s) 260
BfoI RGCGCY 1 cut(s) 12
BmrI ACTGGG 1 cut(s) 131
BmsI GCATC 1 cut(s) 276
BmuI ACTGGG 1 cut(s) 131
Bpu10I CCTNAGC 1 cut(s) 327
BpuEI CTTGAG 1 cut(s) 183
Bsc4I CCNNNNNNNGG 3 cut(s) 88, 102, 103
Bse1I ACTGG 1 cut(s) 126
Bse3DI GCAATG 2 cut(s) 243, 255
BseLI CCNNNNNNNGG 3 cut(s) 88, 102, 103
BseMI GCAATG 2 cut(s) 243, 255
BseMII CTCAG 1 cut(s) 318
BseNI ACTGG 1 cut(s) 126
BseRI GAGGAG 1 cut(s) 186
BseYI CCCAGC 2 cut(s) 102, 373
Bsh1236I CGCG 1 cut(s) 162
BsiHKAI GWGCWC 1 cut(s) 299
BslI CCNNNNNNNGG 3 cut(s) 88, 102, 103
Bsp1286I GDGCHC 1 cut(s) 299
Bsp143I GATC 2 cut(s) 85, 157
BspACI CCGC 2 cut(s) 290, 377
BspCNI CTCAG 1 cut(s) 319
BspFNI CGCG 1 cut(s) 162
BsrDI GCAATG 2 cut(s) 243, 255
BsrI ACTGG 1 cut(s) 126
BssMI GATC 2 cut(s) 85, 157
Bst4CI ACNGT 2 cut(s) 66, 264
Bst6I CTCTTC 1 cut(s) 41
BstC8I GCNNGC 1 cut(s) 364
BstDEI CTNAG 2 cut(s) 215, 327
BstENI CCTNNNNNAGG 1 cut(s) 86
BstFNI CGCG 1 cut(s) 162
BstH2I RGCGCY 1 cut(s) 12
BstHHI GCGC 2 cut(s) 11, 164
BstKTI GATC 2 cut(s) 88, 160
BstMBI GATC 2 cut(s) 85, 157
BstNSI RCATGY 1 cut(s) 366
BstSFI CTRYAG 1 cut(s) 260
BstUI CGCG 1 cut(s) 162
BtsIMutI CAGTG 2 cut(s) 119, 269
Cac8I GCNNGC 1 cut(s) 364
CfoI GCGC 2 cut(s) 11, 164
CviAII CATG 1 cut(s) 363
CviJI RGCY 3 cut(s) 92, 172, 331
CviKI_1 RGCY 3 cut(s) 92, 172, 331
DdeI CTNAG 2 cut(s) 215, 327
DpnI GATC 2 cut(s) 87, 159
DpnII GATC 2 cut(s) 85, 157
Eam1104I CTCTTC 1 cut(s) 41
EarI CTCTTC 1 cut(s) 41
Eco47III AGCGCT 1 cut(s) 10
EcoNI CCTNNNNNAGG 1 cut(s) 86
FaeI CATG 1 cut(s) 366
FaiI YATR 5 cut(s) 205, 257, 310, 352, 364
FatI CATG 1 cut(s) 362
FauI CCCGC 1 cut(s) 283
FbaI TGATCA 1 cut(s) 85
FspBI CTAG 3 cut(s) 129, 300, 335
GlaI GCGC 2 cut(s) 10, 163
GsaI CCCAGC 2 cut(s) 106, 377
HaeII RGCGCY 1 cut(s) 12
HhaI GCGC 2 cut(s) 11, 164
Hin1II CATG 1 cut(s) 366
Hin6I GCGC 2 cut(s) 9, 162
HinP1I GCGC 2 cut(s) 9, 162
HincII GTYRAC 1 cut(s) 139
HindII GTYRAC 1 cut(s) 139
HinfI GANTC 4 cut(s) 33, 107, 219, 237
Hpy166II GTNNAC 2 cut(s) 139, 241
Hpy188I TCNGA 2 cut(s) 19, 370
Hpy8I GTNNAC 2 cut(s) 139, 241
HpyAV CCTTC 1 cut(s) 280
HpyCH4III ACNGT 2 cut(s) 66, 264
HpyCH4V TGCA 3 cut(s) 62, 248, 267
HpyF3I CTNAG 2 cut(s) 215, 327
Hsp92II CATG 1 cut(s) 366
HspAI GCGC 2 cut(s) 9, 162
Ksp22I TGATCA 1 cut(s) 85
Kzo9I GATC 2 cut(s) 85, 157
LmnI GCTCC 1 cut(s) 294
LpnPI CCDG 6 cut(s) 74, 88, 107, 339, 342, 387
LweI GCATC 1 cut(s) 276
MaeI CTAG 3 cut(s) 129, 300, 335
MaeIII GTNAC 1 cut(s) 379
MalI GATC 2 cut(s) 87, 159
MboI GATC 2 cut(s) 85, 157
MboII GAAGA 4 cut(s) 16, 28, 203, 382
MhlI GDGCHC 1 cut(s) 299
MluCI AATT 1 cut(s) 280
MlyI GAGTC 2 cut(s) 27, 246
MmeI TCCRAC 2 cut(s) 48, 214
MnlI CCTC 4 cut(s) 63, 195, 204, 207
MseI TTAA 2 cut(s) 27, 48
MslI CAYNNNNRTG 1 cut(s) 248
MspA1I CMGCKG 1 cut(s) 377
MvnI CGCG 1 cut(s) 162
NdeII GATC 2 cut(s) 85, 157
NlaIII CATG 1 cut(s) 366
NspI RCATGY 1 cut(s) 366
PaeI GCATGC 1 cut(s) 366
PfeI GAWTC 2 cut(s) 107, 219
PleI GAGTC 2 cut(s) 27, 245
PpsI GAGTC 2 cut(s) 27, 245
PshBI ATTAAT 1 cut(s) 27
PspFI CCCAGC 2 cut(s) 102, 373
RseI CAYNNNNRTG 1 cut(s) 248
SaqAI TTAA 2 cut(s) 27, 48
Sau3AI GATC 2 cut(s) 85, 157
SchI GAGTC 2 cut(s) 27, 246
SduI GDGCHC 1 cut(s) 299
SetI ASST 1 cut(s) 174
SfaNI GCATC 1 cut(s) 276
SfcI CTRYAG 1 cut(s) 260
SmiMI CAYNNNNRTG 1 cut(s) 248
SmlI CTYRAG 1 cut(s) 198
SmoI CTYRAG 1 cut(s) 198
SpeI ACTAGT 1 cut(s) 334
SphI GCATGC 1 cut(s) 366
Sse9I AATT 1 cut(s) 280
SsiI CCGC 2 cut(s) 290, 377
SspMI CTAG 3 cut(s) 129, 300, 335
TaaI ACNGT 2 cut(s) 66, 264
TasI AATT 1 cut(s) 280
TfiI GAWTC 2 cut(s) 107, 219
Tru1I TTAA 2 cut(s) 27, 48
Tru9I TTAA 2 cut(s) 27, 48
TscAI CASTG 2 cut(s) 126, 269
TspDTI ATGAA 2 cut(s) 17, 266
TspGWI ACGGA 1 cut(s) 124
TspRI CASTG 2 cut(s) 126, 269
VspI ATTAAT 1 cut(s) 27
XagI CCTNNNNNAGG 1 cut(s) 86
XapI RAATTY 1 cut(s) 280
XceI RCATGY 1 cut(s) 366
XspI CTAG 3 cut(s) 129, 300, 335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.