pycom17g01890

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
N/A
Physical Location & Seq
Reverse (-)
1194082 .. 1194953
872 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 714 bp
ATGATCACCGGCGCAGCCCAAATGGACGGCGCCATCCTCGTCGTCTCCGGCGCCGACGGCCCCATGCCCCAGACCAAAGAGTACGTGTTGCTGGCCAAACAAGTCGGCGTGCCCAATGTGGTCGTTTTCCTGAACAATCAGGATCAGGTGGACGACGAGGAGCTGCTTGAGCTGGTGGAATTGGAGGTCCGGGAGCTCCTGTCGTCGTACGAGTTTCCGGGAGACGATGTGCCGGTGGTTTCCGGCTCGGCATTGCTGGCTCTGGAGGCGTTAATGGCTAACCCGAAAATCAAGAGAGGGGAGAGCGAGTGGGCGGACAAGATTTATGCTTTGATCGATATGTTTCAGAAAACACTCGATGAGGCAATGGCCGGTGACAATGTGGGGCTTCTGCTGAGGGGAATTCAGAAGGGGGATATCCAGAGAGGGATGGCGATTATGTATGTGTTGAAGAAGGAGGAGGGCGGGAGGCATTCCCCATTCTTTGCAGGATACAGGCCACAGTTTTACATGAGGACGACGGATGTGACAGGGAAGGTGACTGGAGTTAAGAATGACAAGGATGAGGAGTCGAAGATGGTGATGCCGGGAGACCGGGTGAAGATGGTGGTGGAGCTTATAATGCCGGTGGCTTGCGAGCAAGGAATGAGGTTTGCTATCAGGGAAGGTGGCAAGACTGTTGGAGGTGGTGTCATCCAGTCCATCATCGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GTP_EFTU GTP_EFTU_D2 GTP_EFTU_D3

Protein Analysis

238

Amino Acids

25.92

Weight (kDa)

4.74

Isoelectric Point (pI)

39.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 1 - 88 2.3e-21 Elongation factor Tu GTP binding domain
GTP_EFTU_D3 PF03143 145 - 235 6e-35 Elongation factor Tu C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 620
AccB1I GGYRCC 2 cut(s) 29, 50
AciI CCGC 2 cut(s) 314, 465
AclWI GGATC 1 cut(s) 150
AcoI YGGCCR 2 cut(s) 93, 369
AcsI RAATTY 1 cut(s) 402
AcyI GRCGYC 2 cut(s) 30, 51
AfaI GTAC 2 cut(s) 83, 209
AflIII ACRYGT 1 cut(s) 84
AgsI TTSAA 1 cut(s) 451
AluBI AGCT 4 cut(s) 163, 172, 196, 616
AluI AGCT 4 cut(s) 163, 172, 196, 616
Alw21I GWGCWC 1 cut(s) 198
Alw26I GTCTC 3 cut(s) 49, 216, 585
AlwI GGATC 1 cut(s) 150
AoxI GGCC 4 cut(s) 58, 93, 369, 497
ApeKI GCWGC 2 cut(s) 14, 163
ApoI RAATTY 1 cut(s) 402
AspLEI GCGC 3 cut(s) 14, 32, 53
AspS9I GGNCC 2 cut(s) 59, 187
AsuC2I CCSGG 4 cut(s) 191, 219, 588, 596
AsuHPI GGTGA 4 cut(s) 386, 550, 592, 610
AvaII GGWCC 1 cut(s) 187
BaeGI GKGCMC 1 cut(s) 114
BalI TGGCCA 1 cut(s) 95
BanI GGYRCC 2 cut(s) 29, 50
BanII GRGCYC 1 cut(s) 198
Bbv12I GWGCWC 1 cut(s) 198
BbvCI CCTCAGC 1 cut(s) 395
BbvI GCAGC 2 cut(s) 26, 150
BccI CCATC 5 cut(s) 41, 424, 571, 598, 710
BceAI ACGGC 2 cut(s) 43, 73
BciVI GTATCC 1 cut(s) 485
BclI TGATCA 1 cut(s) 3
BcnI CCSGG 4 cut(s) 191, 219, 588, 596
BcoDI GTCTC 3 cut(s) 49, 216, 585
BfoI RGCGCY 2 cut(s) 33, 54
BfuI GTATCC 1 cut(s) 485
BisI GCNGC 2 cut(s) 15, 164
BlsI GCNGC 2 cut(s) 16, 165
Bme1390I CCNGG 4 cut(s) 191, 219, 588, 596
Bme18I GGWCC 1 cut(s) 187
BmgT120I GGNCC 2 cut(s) 59, 187
BmiI GGNNCC 3 cut(s) 31, 52, 61
BmrFI CCNGG 4 cut(s) 191, 219, 588, 596
BmsI GCATC 1 cut(s) 573
BpmI CTGGAG 2 cut(s) 284, 564
Bpu10I CCTNAGC 1 cut(s) 395
BpuEI CTTGAG 1 cut(s) 188
BpuMI CCSGG 4 cut(s) 191, 219, 588, 596
Bsa29I ATCGAT 1 cut(s) 336
BsaAI YACGTR 1 cut(s) 85
BsaHI GRCGYC 2 cut(s) 30, 51
BsaI GGTCTC 1 cut(s) 585
Bse118I RCCGGY 4 cut(s) 8, 232, 371, 625
Bse1I ACTGG 2 cut(s) 547, 697
Bse3DI GCAATG 2 cut(s) 251, 372
BseCI ATCGAT 1 cut(s) 336
BseGI GGATG 5 cut(s) 33, 435, 529, 568, 693
BseMI GCAATG 2 cut(s) 251, 372
BseMII CTCAG 1 cut(s) 386
BseNI ACTGG 2 cut(s) 547, 697
BseRI GAGGAG 3 cut(s) 173, 473, 581
BseSI GKGCMC 1 cut(s) 114
BseXI GCAGC 2 cut(s) 26, 150
BshFI GGCC 4 cut(s) 60, 95, 371, 499
BshNI GGYRCC 2 cut(s) 29, 50
BshVI ATCGAT 1 cut(s) 336
BsiHKAI GWGCWC 1 cut(s) 198
BsiWI CGTACG 1 cut(s) 207
BsmAI GTCTC 3 cut(s) 49, 216, 585
BsmBI CGTCTC 2 cut(s) 49, 216
BsmI GAATGC 1 cut(s) 472
BsnI GGCC 4 cut(s) 60, 95, 371, 499
Bso31I GGTCTC 1 cut(s) 585
Bsp1286I GDGCHC 2 cut(s) 114, 198
Bsp143I GATC 3 cut(s) 3, 142, 333
BspACI CCGC 2 cut(s) 314, 465
BspANI GGCC 4 cut(s) 60, 95, 371, 499
BspCNI CTCAG 1 cut(s) 387
BspDI ATCGAT 1 cut(s) 336
BspLI GGNNCC 3 cut(s) 31, 52, 61
BspPI GGATC 1 cut(s) 150
BspT107I GGYRCC 2 cut(s) 29, 50
BspTNI GGTCTC 1 cut(s) 585
BsrDI GCAATG 2 cut(s) 251, 372
BsrFI RCCGGY 4 cut(s) 8, 232, 371, 625
BsrI ACTGG 2 cut(s) 547, 697
BssAI RCCGGY 4 cut(s) 8, 232, 371, 625
BssMI GATC 3 cut(s) 3, 142, 333
BssNI GRCGYC 2 cut(s) 30, 51
Bst4CI ACNGT 2 cut(s) 504, 679
BstACI GRCGYC 2 cut(s) 30, 51
BstBAI YACGTR 1 cut(s) 85
BstC8I GCNNGC 5 cut(s) 93, 110, 258, 634, 638
BstDEI CTNAG 1 cut(s) 395
BstF5I GGATG 5 cut(s) 33, 435, 529, 568, 693
BstH2I RGCGCY 2 cut(s) 33, 54
BstHHI GCGC 3 cut(s) 14, 32, 53
BstKTI GATC 3 cut(s) 6, 145, 336
BstMAI GTCTC 3 cut(s) 49, 216, 585
BstMBI GATC 3 cut(s) 3, 142, 333
BstMWI GCNNNNNNNGC 6 cut(s) 57, 169, 257, 266, 275, 622
BstSCI CCNGG 4 cut(s) 189, 217, 586, 594
BstSLI GKGCMC 1 cut(s) 114
BstV1I GCAGC 2 cut(s) 26, 150
Bsu15I ATCGAT 1 cut(s) 336
BsuI GTATCC 1 cut(s) 485
BsuRI GGCC 4 cut(s) 60, 95, 371, 499
BsuTUI ATCGAT 1 cut(s) 336
BtsCI GGATG 5 cut(s) 33, 435, 529, 568, 693
Cac8I GCNNGC 5 cut(s) 93, 110, 258, 634, 638
CfoI GCGC 3 cut(s) 14, 32, 53
Cfr10I RCCGGY 4 cut(s) 8, 232, 371, 625
Cfr13I GGNCC 2 cut(s) 59, 187
ClaI ATCGAT 1 cut(s) 336
Csp6I GTAC 2 cut(s) 82, 208
CviAII CATG 2 cut(s) 64, 511
CviQI GTAC 2 cut(s) 82, 208
DdeI CTNAG 1 cut(s) 395
DinI GGCGCC 2 cut(s) 31, 52
DpnI GATC 3 cut(s) 5, 144, 335
DpnII GATC 3 cut(s) 3, 142, 333
EaeI YGGCCR 2 cut(s) 93, 369
EciI GGCGGA 1 cut(s) 329
Ecl136II GAGCTC 1 cut(s) 196
Eco24I GRGCYC 1 cut(s) 198
Eco31I GGTCTC 1 cut(s) 585
Eco32I GATATC 1 cut(s) 418
Eco47I GGWCC 1 cut(s) 187
Eco53kI GAGCTC 1 cut(s) 196
EcoICRI GAGCTC 1 cut(s) 196
EcoRI GAATTC 1 cut(s) 402
EcoRV GATATC 1 cut(s) 418
EcoT38I GRGCYC 1 cut(s) 198
EgeI GGCGCC 2 cut(s) 31, 52
EheI GGCGCC 2 cut(s) 31, 52
Esp3I CGTCTC 2 cut(s) 49, 216
FaeI CATG 2 cut(s) 67, 514
FaiI YATR 7 cut(s) 65, 327, 341, 440, 444, 512, 620
FatI CATG 2 cut(s) 63, 510
FauI CCCGC 1 cut(s) 458
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 2 cut(s) 15, 164
FokI GGATG 5 cut(s) 20, 442, 536, 575, 680
FriOI GRGCYC 1 cut(s) 198
Fsp4HI GCNGC 2 cut(s) 15, 164
GlaI GCGC 3 cut(s) 13, 31, 52
GluI GCNGC 2 cut(s) 15, 164
GsuI CTGGAG 2 cut(s) 284, 564
HaeII RGCGCY 2 cut(s) 33, 54
HaeIII GGCC 4 cut(s) 60, 95, 371, 499
HhaI GCGC 3 cut(s) 14, 32, 53
Hin1I GRCGYC 2 cut(s) 30, 51
Hin1II CATG 2 cut(s) 67, 514
Hin6I GCGC 3 cut(s) 12, 30, 51
HinP1I GCGC 3 cut(s) 12, 30, 51
HinfI GANTC 1 cut(s) 569
HphI GGTGA 4 cut(s) 386, 550, 592, 610
Hpy166II GTNNAC 1 cut(s) 151
Hpy188I TCNGA 2 cut(s) 348, 408
Hpy188III TCNNGA 5 cut(s) 130, 140, 263, 292, 421
Hpy8I GTNNAC 1 cut(s) 151
Hpy99I CGWCG 5 cut(s) 44, 59, 158, 208, 523
HpyAV CCTTC 4 cut(s) 403, 448, 529, 659
HpyCH4III ACNGT 2 cut(s) 504, 679
HpyCH4IV ACGT 1 cut(s) 84
HpyCH4V TGCA 1 cut(s) 488
HpyF10VI GCNNNNNNNGC 6 cut(s) 57, 169, 257, 266, 275, 622
HpyF3I CTNAG 1 cut(s) 395
HpySE526I ACGT 1 cut(s) 84
Hsp92I GRCGYC 2 cut(s) 30, 51
Hsp92II CATG 2 cut(s) 67, 514
HspAI GCGC 3 cut(s) 12, 30, 51
KasI GGCGCC 2 cut(s) 29, 50
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 3 cut(s) 3, 142, 333
LmnI GCTCC 4 cut(s) 160, 193, 201, 613
Lsp1109I GCAGC 2 cut(s) 26, 150
LweI GCATC 1 cut(s) 573
MaeII ACGT 1 cut(s) 84
MaeIII GTNAC 3 cut(s) 374, 526, 538
MalI GATC 3 cut(s) 5, 144, 335
MboI GATC 3 cut(s) 3, 142, 333
MboII GAAGA 3 cut(s) 463, 586, 613
MhlI GDGCHC 2 cut(s) 114, 198
MlsI TGGCCA 1 cut(s) 95
MluCI AATT 2 cut(s) 179, 402
MluNI TGGCCA 1 cut(s) 95
Mly113I GGCGCC 2 cut(s) 30, 51
MlyI GAGTC 1 cut(s) 578
MmeI TCCRAC 1 cut(s) 661
Mox20I TGGCCA 1 cut(s) 95
MscI TGGCCA 1 cut(s) 95
MseI TTAA 2 cut(s) 272, 549
Msp20I TGGCCA 1 cut(s) 95
MspR9I CCNGG 4 cut(s) 191, 219, 588, 596
Mva1269I GAATGC 1 cut(s) 472
MwoI GCNNNNNNNGC 6 cut(s) 57, 169, 257, 266, 275, 622
NarI GGCGCC 2 cut(s) 30, 51
NciI CCSGG 4 cut(s) 191, 219, 588, 596
NdeII GATC 3 cut(s) 3, 142, 333
NlaIII CATG 2 cut(s) 67, 514
NlaIV GGNNCC 3 cut(s) 31, 52, 61
NmeAIII GCCGAG 1 cut(s) 227
NmuCI GTSAC 3 cut(s) 374, 526, 538
PctI GAATGC 1 cut(s) 472
Pfl23II CGTACG 1 cut(s) 207
PfoI TCCNGGA 2 cut(s) 189, 217
PkrI GCNGC 2 cut(s) 16, 165
PleI GAGTC 1 cut(s) 577
PluTI GGCGCC 2 cut(s) 33, 54
PpsI GAGTC 1 cut(s) 577
Ppu21I YACGTR 1 cut(s) 85
PsiI TTATAA 1 cut(s) 620
Psp124BI GAGCTC 1 cut(s) 198
PspLI CGTACG 1 cut(s) 207
PspN4I GGNNCC 3 cut(s) 31, 52, 61
PspPI GGNCC 2 cut(s) 59, 187
RsaI GTAC 2 cut(s) 83, 209
RsaNI GTAC 2 cut(s) 82, 208
SacI GAGCTC 1 cut(s) 198
SaqAI TTAA 2 cut(s) 272, 549
SatI GCNGC 2 cut(s) 15, 164
Sau3AI GATC 3 cut(s) 3, 142, 333
Sau96I GGNCC 2 cut(s) 59, 187
SchI GAGTC 1 cut(s) 578
ScrFI CCNGG 4 cut(s) 191, 219, 588, 596
SduI GDGCHC 2 cut(s) 114, 198
SfaNI GCATC 1 cut(s) 573
SfoI GGCGCC 2 cut(s) 31, 52
SgrAI CRCCGGYG 1 cut(s) 8
SinI GGWCC 1 cut(s) 187
SmlI CTYRAG 1 cut(s) 167
SmoI CTYRAG 1 cut(s) 167
Sse9I AATT 2 cut(s) 179, 402
SsiI CCGC 2 cut(s) 314, 465
SspDI GGCGCC 2 cut(s) 29, 50
SstI GAGCTC 1 cut(s) 198
StyD4I CCNGG 4 cut(s) 189, 217, 586, 594
TaaI ACNGT 2 cut(s) 504, 679
TaiI ACGT 1 cut(s) 87
TaqI TCGA 4 cut(s) 336, 357, 572, 708
TasI AATT 2 cut(s) 179, 402
Tru1I TTAA 2 cut(s) 272, 549
Tru9I TTAA 2 cut(s) 272, 549
TseFI GTSAC 3 cut(s) 374, 526, 538
TseI GCWGC 2 cut(s) 14, 163
Tsp45I GTSAC 3 cut(s) 374, 526, 538
TspGWI ACGGA 1 cut(s) 536
VpaK11BI GGWCC 1 cut(s) 187
XapI RAATTY 1 cut(s) 402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.