pycom17g03590

Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
N/A
Physical Location & Seq
Forward (+)
2407882 .. 2408628
747 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 747 bp
ATGGCGGCATTCCTCATTGTCCTCCTCACATATCTACTAGCTCTCCCCACTCTCCCCGCCGCCGAATCCCGACTCTACACCAACTACTACCAAAAGTCGTGCCCGAGATTCGCCCAAATCGTCCAAGACACCGTCACCAACAAGCAGATAACGAACCCCACCACCGCCGCCGGCACCCTCCGCCTCTTCTTCCACGACTGCCTTCACAACGGCTGCGACGGCTCCATTCTCCTCTCCTCCACCCCCTTCAACAAGGCCGAGCGCGACGCCGACATCAACCTCTCCCTCCCCGGAGACGCCTTCGACGTCGTTGTCCGGGCCAAGACCGCCCTCGAGCTCGCCTGCCCCAACACCGTCTCCTGCGCCGACATCCTTGCCGTCGCCACCCGCGATCTCGTAACCATGATGGGTGGCCCCTTCTACAACGTCCCCCTCGGCCGCCGCGATGGCCGCTCCTCAAAAGCCTCCGCCGTCGAAGGCACCCTCCCCCGCCCCGCCATGTCCATCTCCCAGCTCATCGATCGATCTTCGGGTCCAGGGGCTTCTCCGTCCAGGAAATGGTCGCCCTCAGCGGGGCCCACACCATCGGGTTCTCCCACTGCAGCGAGTTCAGCTCGGCGATCTACAACTACAGCAAGTCGGCGCAGTCCGACCCGAATTACAACCCGAGATTCGCATCCGGGTTGCAGCAGGCCTGCGCGGATATCAAGAAAAACCCGACGCTCTCCGTCTTTAACGACGTTATGA

Protein Analysis

249

Amino Acids

26.48

Weight (kDa)

10.16

Isoelectric Point (pI)

70.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 41 - 175 1.3e-40 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 311
AatII GACGTC 1 cut(s) 309
AccB1I GGYRCC 2 cut(s) 173, 479
AccB7I CCANNNNNTGG 1 cut(s) 558
AccBSI CCGCTC 1 cut(s) 453
AccII CGCG 4 cut(s) 264, 390, 444, 700
AcoI YGGCCR 2 cut(s) 436, 448
AcyI GRCGYC 3 cut(s) 267, 297, 306
AfiI CCNNNNNNNGG 3 cut(s) 558, 572, 573
AgsI TTSAA 1 cut(s) 250
AjnI CCWGG 2 cut(s) 535, 551
AluBI AGCT 4 cut(s) 41, 337, 514, 614
AluI AGCT 4 cut(s) 41, 337, 514, 614
Alw21I GWGCWC 1 cut(s) 339
Alw26I GTCTC 2 cut(s) 288, 361
Ama87I CYCGRG 3 cut(s) 103, 332, 666
AoxI GGCC 7 cut(s) 255, 318, 412, 436, 448, 575, 692
ApaI GGGCCC 1 cut(s) 579
ApeKI GCWGC 3 cut(s) 213, 602, 687
AspLEI GCGC 4 cut(s) 264, 365, 645, 700
AspS9I GGNCC 5 cut(s) 318, 413, 533, 575, 576
AsuC2I CCSGG 3 cut(s) 291, 317, 681
AsuHPI GGTGA 1 cut(s) 127
AvaI CYCGRG 3 cut(s) 103, 332, 666
AvaII GGWCC 1 cut(s) 533
BaeGI GKGCMC 2 cut(s) 104, 579
BanI GGYRCC 2 cut(s) 173, 479
BanII GRGCYC 2 cut(s) 339, 579
Bbv12I GWGCWC 1 cut(s) 339
BbvCI CCTCAGC 1 cut(s) 568
BbvI GCAGC 3 cut(s) 200, 614, 699
BccI CCATC 4 cut(s) 400, 440, 512, 592
BceAI ACGGC 4 cut(s) 226, 235, 362, 455
BcgI CGANNNNNNTGC 2 cut(s) 356, 390
BciT130I CCWGG 2 cut(s) 537, 553
BcnI CCSGG 3 cut(s) 291, 317, 681
BcoDI GTCTC 2 cut(s) 288, 361
BfaI CTAG 1 cut(s) 38
BfmI CTRYAG 2 cut(s) 600, 630
BglI GCCNNNNNGGC 1 cut(s) 447
BisI GCNGC 9 cut(s) 6, 60, 168, 214, 439, 442, 451, 603, 688
BlsI GCNGC 9 cut(s) 7, 61, 169, 215, 440, 443, 452, 604, 689
Bme1390I CCNGG 5 cut(s) 291, 317, 537, 553, 681
Bme18I GGWCC 1 cut(s) 533
BmeT110I CYCGRG 3 cut(s) 103, 332, 666
BmgT120I GGNCC 5 cut(s) 318, 413, 533, 575, 576
BmiI GGNNCC 7 cut(s) 175, 223, 415, 481, 534, 576, 577
BmrFI CCNGG 5 cut(s) 291, 317, 537, 553, 681
BmsI GCATC 1 cut(s) 685
BplI GAGNNNNNCTC 2 cut(s) 598, 630
Bpu10I CCTNAGC 1 cut(s) 568
BpuMI CCSGG 3 cut(s) 291, 317, 681
Bsa29I ATCGAT 2 cut(s) 519, 523
BsaBI GATNNNNATC 1 cut(s) 675
BsaHI GRCGYC 3 cut(s) 267, 297, 306
BsaJI CCNNGG 3 cut(s) 289, 433, 536
BsaXI ACNNNNNCTCC 4 cut(s) 27, 57, 341, 371
Bsc4I CCNNNNNNNGG 3 cut(s) 558, 572, 573
Bse118I RCCGGY 1 cut(s) 170
Bse8I GATNNNNATC 1 cut(s) 675
BseBI CCWGG 2 cut(s) 537, 553
BseCI ATCGAT 2 cut(s) 519, 523
BseDI CCNNGG 3 cut(s) 289, 433, 536
BseGI GGATG 2 cut(s) 369, 676
BseJI GATNNNNATC 1 cut(s) 675
BseLI CCNNNNNNNGG 3 cut(s) 558, 572, 573
BseMII CTCAG 1 cut(s) 582
BseRI GAGGAG 4 cut(s) 14, 221, 226, 445
BseSI GKGCMC 2 cut(s) 104, 579
BseX3I CGGCCG 1 cut(s) 436
BseXI GCAGC 3 cut(s) 200, 614, 699
BseYI CCCAGC 1 cut(s) 510
Bsh1236I CGCG 4 cut(s) 264, 390, 444, 700
Bsh1285I CGRYCG 2 cut(s) 439, 523
BshFI GGCC 7 cut(s) 257, 320, 414, 438, 450, 577, 694
BshNI GGYRCC 2 cut(s) 173, 479
BshVI ATCGAT 2 cut(s) 519, 523
BsiEI CGRYCG 2 cut(s) 439, 523
BsiHKAI GWGCWC 1 cut(s) 339
BsiHKCI CYCGRG 3 cut(s) 103, 332, 666
BsiSI CCGG 4 cut(s) 171, 291, 316, 680
BslFI GGGAC 1 cut(s) 413
BslI CCNNNNNNNGG 3 cut(s) 558, 572, 573
BsmAI GTCTC 2 cut(s) 288, 361
BsmBI CGTCTC 2 cut(s) 288, 361
BsmFI GGGAC 1 cut(s) 413
BsmI GAATGC 1 cut(s) 8
BsnI GGCC 7 cut(s) 257, 320, 414, 438, 450, 577, 694
BsoBI CYCGRG 3 cut(s) 103, 332, 666
Bsp120I GGGCCC 1 cut(s) 575
Bsp1286I GDGCHC 3 cut(s) 104, 339, 579
Bsp143I GATC 4 cut(s) 391, 520, 524, 620
BspANI GGCC 7 cut(s) 257, 320, 414, 438, 450, 577, 694
BspCNI CTCAG 1 cut(s) 581
BspDI ATCGAT 2 cut(s) 519, 523
BspFNI CGCG 4 cut(s) 264, 390, 444, 700
BspLI GGNNCC 7 cut(s) 175, 223, 415, 481, 534, 576, 577
BspMAI CTGCAG 1 cut(s) 604
BspT107I GGYRCC 2 cut(s) 173, 479
BsrBI CCGCTC 1 cut(s) 453
BsrFI RCCGGY 1 cut(s) 170
BssAI RCCGGY 1 cut(s) 170
BssECI CCNNGG 3 cut(s) 289, 433, 536
BssMI GATC 4 cut(s) 391, 520, 524, 620
BssNI GRCGYC 3 cut(s) 267, 297, 306
Bst2UI CCWGG 2 cut(s) 537, 553
Bst4CI ACNGT 2 cut(s) 133, 355
Bst6I CTCTTC 1 cut(s) 191
BstACI GRCGYC 3 cut(s) 267, 297, 306
BstC8I GCNNGC 5 cut(s) 172, 339, 343, 692, 696
BstDEI CTNAG 1 cut(s) 568
BstF5I GGATG 2 cut(s) 369, 676
BstFNI CGCG 4 cut(s) 264, 390, 444, 700
BstHHI GCGC 4 cut(s) 264, 365, 645, 700
BstKTI GATC 4 cut(s) 394, 523, 527, 623
BstMAI GTCTC 2 cut(s) 288, 361
BstMBI GATC 4 cut(s) 391, 520, 524, 620
BstMCI CGRYCG 2 cut(s) 439, 523
BstMWI GCNNNNNNNGC 6 cut(s) 180, 219, 326, 447, 450, 611
BstNI CCWGG 2 cut(s) 537, 553
BstSCI CCNGG 5 cut(s) 289, 315, 535, 551, 679
BstSFI CTRYAG 2 cut(s) 600, 630
BstSLI GKGCMC 2 cut(s) 104, 579
BstUI CGCG 4 cut(s) 264, 390, 444, 700
BstV1I GCAGC 3 cut(s) 200, 614, 699
BstZI CGGCCG 1 cut(s) 436
Bsu15I ATCGAT 2 cut(s) 519, 523
BsuRI GGCC 7 cut(s) 257, 320, 414, 438, 450, 577, 694
BsuTUI ATCGAT 2 cut(s) 519, 523
BtgZI GCGATG 1 cut(s) 459
BtsCI GGATG 2 cut(s) 369, 676
BtsI GCAGTG 1 cut(s) 597
BtsIMutI CAGTG 1 cut(s) 597
Cac8I GCNNGC 5 cut(s) 172, 339, 343, 692, 696
CfoI GCGC 4 cut(s) 264, 365, 645, 700
Cfr10I RCCGGY 1 cut(s) 170
Cfr13I GGNCC 5 cut(s) 318, 413, 533, 575, 576
ClaI ATCGAT 2 cut(s) 519, 523
CseI GACGC 3 cut(s) 275, 305, 729
CviAII CATG 2 cut(s) 403, 499
DdeI CTNAG 1 cut(s) 568
DpnI GATC 4 cut(s) 393, 522, 526, 622
DpnII GATC 4 cut(s) 391, 520, 524, 620
DrdI GACNNNNNNGTC 1 cut(s) 311
DseDI GACNNNNNNGTC 1 cut(s) 311
EaeI YGGCCR 2 cut(s) 436, 448
EagI CGGCCG 1 cut(s) 436
Eam1104I CTCTTC 1 cut(s) 191
EarI CTCTTC 1 cut(s) 191
EciI GGCGGA 2 cut(s) 170, 457
Ecl136II GAGCTC 1 cut(s) 337
EclXI CGGCCG 1 cut(s) 436
Eco147I AGGCCT 1 cut(s) 694
Eco24I GRGCYC 2 cut(s) 339, 579
Eco32I GATATC 1 cut(s) 705
Eco47I GGWCC 1 cut(s) 533
Eco52I CGGCCG 1 cut(s) 436
Eco53kI GAGCTC 1 cut(s) 337
Eco88I CYCGRG 3 cut(s) 103, 332, 666
EcoICRI GAGCTC 1 cut(s) 337
EcoO109I RGGNCCY 1 cut(s) 575
EcoRII CCWGG 2 cut(s) 535, 551
EcoRV GATATC 1 cut(s) 705
EcoT38I GRGCYC 2 cut(s) 339, 579
Esp3I CGTCTC 2 cut(s) 288, 361
FaeI CATG 2 cut(s) 406, 502
FaiI YATR 4 cut(s) 31, 404, 500, 745
FaqI GGGAC 1 cut(s) 413
FatI CATG 2 cut(s) 402, 498
FauI CCCGC 5 cut(s) 64, 395, 497, 502, 565
Fnu4HI GCNGC 9 cut(s) 6, 60, 168, 214, 439, 442, 451, 603, 688
FokI GGATG 2 cut(s) 356, 663
FriOI GRGCYC 2 cut(s) 339, 579
Fsp4HI GCNGC 9 cut(s) 6, 60, 168, 214, 439, 442, 451, 603, 688
FspBI CTAG 1 cut(s) 38
GlaI GCGC 4 cut(s) 263, 364, 644, 699
GluI GCNGC 9 cut(s) 6, 60, 168, 214, 439, 442, 451, 603, 688
GsaI CCCAGC 1 cut(s) 514
HaeIII GGCC 7 cut(s) 257, 320, 414, 438, 450, 577, 694
HapII CCGG 4 cut(s) 171, 291, 316, 680
HgaI GACGC 3 cut(s) 275, 305, 729
HhaI GCGC 4 cut(s) 264, 365, 645, 700
Hin1I GRCGYC 3 cut(s) 267, 297, 306
Hin1II CATG 2 cut(s) 406, 502
Hin6I GCGC 4 cut(s) 262, 363, 643, 698
HinP1I GCGC 4 cut(s) 262, 363, 643, 698
HinfI GANTC 4 cut(s) 65, 72, 108, 671
HpaII CCGG 4 cut(s) 171, 291, 316, 680
HphI GGTGA 1 cut(s) 127
Hpy188I TCNGA 1 cut(s) 651
Hpy188III TCNNGA 2 cut(s) 69, 708
Hpy99I CGWCG 8 cut(s) 221, 269, 308, 311, 383, 476, 723, 742
HpyAV CCTTC 5 cut(s) 212, 256, 310, 427, 470
HpyCH4III ACNGT 2 cut(s) 133, 355
HpyCH4IV ACGT 3 cut(s) 306, 426, 740
HpyCH4V TGCA 2 cut(s) 602, 687
HpyF10VI GCNNNNNNNGC 6 cut(s) 180, 219, 326, 447, 450, 611
HpyF3I CTNAG 1 cut(s) 568
HpySE526I ACGT 3 cut(s) 306, 426, 740
Hsp92I GRCGYC 3 cut(s) 267, 297, 306
Hsp92II CATG 2 cut(s) 406, 502
HspAI GCGC 4 cut(s) 262, 363, 643, 698
KroI GCCGGC 1 cut(s) 170
KroNI GCCGGC 1 cut(s) 172
Kzo9I GATC 4 cut(s) 391, 520, 524, 620
LmnI GCTCC 2 cut(s) 227, 458
Lsp1109I GCAGC 3 cut(s) 200, 614, 699
LweI GCATC 1 cut(s) 685
MaeI CTAG 1 cut(s) 38
MaeII ACGT 3 cut(s) 306, 426, 740
MaeIII GTNAC 2 cut(s) 133, 397
MalI GATC 4 cut(s) 393, 522, 526, 622
MbiI CCGCTC 1 cut(s) 453
MboI GATC 4 cut(s) 391, 520, 524, 620
MboII GAAGA 3 cut(s) 178, 181, 519
MhlI GDGCHC 3 cut(s) 104, 339, 579
MluCI AATT 1 cut(s) 657
MlyI GAGTC 1 cut(s) 66
MmeI TCCRAC 1 cut(s) 674
MroNI GCCGGC 1 cut(s) 170
MseI TTAA 1 cut(s) 734
MspA1I CMGCKG 1 cut(s) 572
MspI CCGG 4 cut(s) 171, 291, 316, 680
MspR9I CCNGG 5 cut(s) 291, 317, 537, 553, 681
Mva1269I GAATGC 1 cut(s) 8
MvaI CCWGG 2 cut(s) 537, 553
MvnI CGCG 4 cut(s) 264, 390, 444, 700
MwoI GCNNNNNNNGC 6 cut(s) 180, 219, 326, 447, 450, 611
NaeI GCCGGC 1 cut(s) 172
NciI CCSGG 3 cut(s) 291, 317, 681
NdeII GATC 4 cut(s) 391, 520, 524, 620
NgoMIV GCCGGC 1 cut(s) 170
NlaIII CATG 2 cut(s) 406, 502
NlaIV GGNNCC 7 cut(s) 175, 223, 415, 481, 534, 576, 577
NmeAIII GCCGAG 3 cut(s) 283, 414, 595
NmuCI GTSAC 1 cut(s) 133
PaeR7I CTCGAG 1 cut(s) 332
PceI AGGCCT 1 cut(s) 694
PcsI WCGNNNNNNNCGW 4 cut(s) 117, 303, 387, 441
PctI GAATGC 1 cut(s) 8
PdiI GCCGGC 1 cut(s) 172
PfeI GAWTC 3 cut(s) 65, 108, 671
PflFI GACNNNGTC 1 cut(s) 131
PflMI CCANNNNNTGG 1 cut(s) 558
PfoI TCCNGGA 1 cut(s) 551
PkrI GCNGC 9 cut(s) 7, 61, 169, 215, 440, 443, 452, 604, 689
Ple19I CGATCG 1 cut(s) 523
PleI GAGTC 1 cut(s) 66
PpsI GAGTC 1 cut(s) 66
Psp124BI GAGCTC 1 cut(s) 339
Psp6I CCWGG 2 cut(s) 535, 551
PspFI CCCAGC 1 cut(s) 510
PspGI CCWGG 2 cut(s) 535, 551
PspN4I GGNNCC 7 cut(s) 175, 223, 415, 481, 534, 576, 577
PspOMI GGGCCC 1 cut(s) 575
PspPI GGNCC 5 cut(s) 318, 413, 533, 575, 576
PspXI VCTCGAGB 1 cut(s) 332
PstI CTGCAG 1 cut(s) 604
PsyI GACNNNGTC 1 cut(s) 131
PvuI CGATCG 1 cut(s) 523
SacI GAGCTC 1 cut(s) 339
SaqAI TTAA 1 cut(s) 734
SatI GCNGC 9 cut(s) 6, 60, 168, 214, 439, 442, 451, 603, 688
Sau3AI GATC 4 cut(s) 391, 520, 524, 620
Sau96I GGNCC 5 cut(s) 318, 413, 533, 575, 576
SchI GAGTC 1 cut(s) 66
ScrFI CCNGG 5 cut(s) 291, 317, 537, 553, 681
SduI GDGCHC 3 cut(s) 104, 339, 579
SetI ASST 8 cut(s) 43, 282, 309, 339, 429, 516, 616, 743
SfaNI GCATC 1 cut(s) 685
SfcI CTRYAG 2 cut(s) 600, 630
Sfr274I CTCGAG 1 cut(s) 332
SinI GGWCC 1 cut(s) 533
SlaI CTCGAG 1 cut(s) 332
SmlI CTYRAG 1 cut(s) 332
SmoI CTYRAG 1 cut(s) 332
Sse9I AATT 1 cut(s) 657
SseBI AGGCCT 1 cut(s) 694
SspMI CTAG 1 cut(s) 38
SstI GAGCTC 1 cut(s) 339
StuI AGGCCT 1 cut(s) 694
StyD4I CCNGG 5 cut(s) 289, 315, 535, 551, 679
TaaI ACNGT 2 cut(s) 133, 355
TaiI ACGT 3 cut(s) 309, 429, 743
TaqI TCGA 5 cut(s) 303, 333, 474, 519, 523
TasI AATT 1 cut(s) 657
TauI GCSGC 6 cut(s) 8, 62, 170, 441, 444, 453
TfiI GAWTC 3 cut(s) 65, 108, 671
Tru1I TTAA 1 cut(s) 734
Tru9I TTAA 1 cut(s) 734
TscAI CASTG 1 cut(s) 604
TseFI GTSAC 1 cut(s) 133
TseI GCWGC 3 cut(s) 213, 602, 687
Tsp45I GTSAC 1 cut(s) 133
TspGWI ACGGA 2 cut(s) 537, 717
TspRI CASTG 1 cut(s) 604
Tth111I GACNNNGTC 1 cut(s) 131
Van91I CCANNNNNTGG 1 cut(s) 558
VpaK11BI GGWCC 1 cut(s) 533
XhoI CTCGAG 1 cut(s) 332
XspI CTAG 1 cut(s) 38
ZraI GACGTC 1 cut(s) 307
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.