pycom17g09050

traB domain-containing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
N/A
Physical Location & Seq
Forward (+)
6768260 .. 6769248
989 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 531 bp
ATGGGTTCCATCACGCGCCGCGTCTTCCTCTCTCTCCGCCGTTCTCCGAACACGCTCGCGCACTTCCCCGCCGCAAGGAAATTCACATGTGCGTCCTCTACCATCGCCGGCAGTCCGTGTCAGAGCGACCCGTTACGGAGCGTGGTAGCTCTTACTTGTAAGTCCACTGCTACGGGCGGTGTTTGCGAAGTGTACCTGGTGGGAACGTGTCACATTTCGGCGAAATCGTGCAGAGAAGTGGAAGCAGTGATCAGACATTTGAAACCAGAGGTTGTGTTCTTGGAGTTATGCTCGAGTCGAGTGGCTGCACTCACCCCTCCAAATTTGAAGGATTTGAAGGAACTGACAATGGAAGAAATGATCGAAATGTGGAAGAAAAATCATAATGCCCTTGAAATTTATGGCTGGATCCTTTCCAAGGGTTTAAGTGAGCTTGAGGTGTTTCCCGGTTCTGAGTTTGGGGTAGCATATGAAGAAGCAAGGAAGTATGGCGGTAAGGTGATACTTGGTGATCGCCCAGTGCAGGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TraB

Protein Analysis

177

Amino Acids

19.33

Weight (kDa)

8.69

Isoelectric Point (pI)

54.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TraB_PrgY_gumN PF01963 58 - 104 7.4e-06 TraB/PrgY/gumN family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 514
AccII CGCG 3 cut(s) 16, 21, 59
AciI CCGC 6 cut(s) 19, 37, 69, 72, 177, 492
AclWI GGATC 2 cut(s) 403, 416
AcsI RAATTY 3 cut(s) 80, 322, 396
AfaI GTAC 1 cut(s) 194
AfiI CCNNNNNNNGG 3 cut(s) 75, 418, 523
AflIII ACRYGT 2 cut(s) 86, 206
AgsI TTSAA 4 cut(s) 262, 328, 337, 395
AjnI CCWGG 1 cut(s) 195
AluBI AGCT 2 cut(s) 149, 433
AluI AGCT 2 cut(s) 149, 433
AlwI GGATC 2 cut(s) 403, 416
Ama87I CYCGRG 1 cut(s) 292
ApeKI GCWGC 1 cut(s) 305
ApoI RAATTY 3 cut(s) 80, 322, 396
AspLEI GCGC 2 cut(s) 18, 61
AsuC2I CCSGG 1 cut(s) 447
AsuHPI GGTGA 3 cut(s) 304, 511, 521
AvaI CYCGRG 1 cut(s) 292
BamHI GGATCC 1 cut(s) 408
BbsI GAAGAC 1 cut(s) 16
BbvI GCAGC 1 cut(s) 292
BccI CCATC 2 cut(s) 17, 110
BceAI ACGGC 1 cut(s) 24
BciT130I CCWGG 1 cut(s) 197
BclI TGATCA 1 cut(s) 249
BcnI CCSGG 1 cut(s) 447
BfuAI ACCTGC 1 cut(s) 514
BisI GCNGC 3 cut(s) 19, 72, 306
BlsI GCNGC 3 cut(s) 20, 73, 307
Bme1390I CCNGG 2 cut(s) 197, 447
BmeT110I CYCGRG 1 cut(s) 292
BmiI GGNNCC 2 cut(s) 7, 410
BmrFI CCNGG 2 cut(s) 197, 447
BmrI ACTGGG 1 cut(s) 512
BmuI ACTGGG 1 cut(s) 512
BpiI GAAGAC 1 cut(s) 16
BplI GAGNNNNNCTC 2 cut(s) 275, 307
BpuEI CTTGAG 1 cut(s) 455
BpuMI CCSGG 1 cut(s) 447
BsaJI CCNNGG 1 cut(s) 417
Bsc4I CCNNNNNNNGG 3 cut(s) 75, 418, 523
Bse118I RCCGGY 1 cut(s) 107
Bse1I ACTGG 1 cut(s) 518
BseBI CCWGG 1 cut(s) 197
BseDI CCNNGG 1 cut(s) 417
BseLI CCNNNNNNNGG 3 cut(s) 75, 418, 523
BseMII CTCAG 1 cut(s) 444
BseNI ACTGG 1 cut(s) 518
BseXI GCAGC 1 cut(s) 292
BsgI GTGCAG 2 cut(s) 250, 291
Bsh1236I CGCG 3 cut(s) 16, 21, 59
BsiHKCI CYCGRG 1 cut(s) 292
BsiSI CCGG 2 cut(s) 108, 447
BslI CCNNNNNNNGG 3 cut(s) 75, 418, 523
BsoBI CYCGRG 1 cut(s) 292
Bsp143I GATC 4 cut(s) 249, 360, 408, 511
BspACI CCGC 6 cut(s) 19, 37, 69, 72, 177, 492
BspCNI CTCAG 1 cut(s) 445
BspFNI CGCG 3 cut(s) 16, 21, 59
BspLI GGNNCC 2 cut(s) 7, 410
BspMI ACCTGC 1 cut(s) 514
BspPI GGATC 2 cut(s) 403, 416
BsrFI RCCGGY 1 cut(s) 107
BsrI ACTGG 1 cut(s) 518
BssAI RCCGGY 1 cut(s) 107
BssECI CCNNGG 1 cut(s) 417
BssMI GATC 4 cut(s) 249, 360, 408, 511
BssT1I CCWWGG 1 cut(s) 417
Bst2UI CCWGG 1 cut(s) 197
BstC8I GCNNGC 2 cut(s) 57, 109
BstDEI CTNAG 1 cut(s) 453
BstENI CCTNNNNNAGG 1 cut(s) 416
BstFNI CGCG 3 cut(s) 16, 21, 59
BstHHI GCGC 2 cut(s) 18, 61
BstKTI GATC 4 cut(s) 252, 363, 411, 514
BstMBI GATC 4 cut(s) 249, 360, 408, 511
BstMWI GCNNNNNNNGC 1 cut(s) 183
BstNI CCWGG 1 cut(s) 197
BstNSI RCATGY 1 cut(s) 90
BstSCI CCNGG 2 cut(s) 195, 445
BstUI CGCG 3 cut(s) 16, 21, 59
BstV1I GCAGC 1 cut(s) 292
BstV2I GAAGAC 1 cut(s) 16
BstX2I RGATCY 1 cut(s) 408
BstYI RGATCY 1 cut(s) 408
BtgZI GCGATG 1 cut(s) 88
BtsI GCAGTG 2 cut(s) 165, 252
BtsIMutI CAGTG 3 cut(s) 165, 252, 525
BveI ACCTGC 1 cut(s) 514
Cac8I GCNNGC 2 cut(s) 57, 109
CfoI GCGC 2 cut(s) 18, 61
Cfr10I RCCGGY 1 cut(s) 107
CseI GACGC 2 cut(s) 10, 81
CsiI ACCWGGT 1 cut(s) 195
Csp6I GTAC 1 cut(s) 193
CviAII CATG 1 cut(s) 87
CviJI RGCY 4 cut(s) 149, 305, 405, 433
CviKI_1 RGCY 4 cut(s) 149, 305, 405, 433
CviQI GTAC 1 cut(s) 193
DdeI CTNAG 1 cut(s) 453
DpnI GATC 4 cut(s) 251, 362, 410, 513
DpnII GATC 4 cut(s) 249, 360, 408, 511
EciI GGCGGA 1 cut(s) 26
Eco130I CCWWGG 1 cut(s) 417
Eco88I CYCGRG 1 cut(s) 292
EcoNI CCTNNNNNAGG 1 cut(s) 416
EcoRII CCWGG 1 cut(s) 195
EcoT14I CCWWGG 1 cut(s) 417
ErhI CCWWGG 1 cut(s) 417
FaeI CATG 1 cut(s) 90
FaiI YATR 8 cut(s) 88, 289, 384, 402, 469, 471, 489, 529
FatI CATG 1 cut(s) 86
FauI CCCGC 1 cut(s) 76
FauNDI CATATG 1 cut(s) 469
FbaI TGATCA 1 cut(s) 249
Fnu4HI GCNGC 3 cut(s) 19, 72, 306
Fsp4HI GCNGC 3 cut(s) 19, 72, 306
GlaI GCGC 2 cut(s) 17, 60
GluI GCNGC 3 cut(s) 19, 72, 306
HapII CCGG 2 cut(s) 108, 447
HgaI GACGC 2 cut(s) 10, 81
HhaI GCGC 2 cut(s) 18, 61
Hin1II CATG 1 cut(s) 90
Hin6I GCGC 2 cut(s) 16, 59
HinP1I GCGC 2 cut(s) 16, 59
HinfI GANTC 1 cut(s) 295
HpaII CCGG 2 cut(s) 108, 447
HphI GGTGA 3 cut(s) 304, 511, 521
Hpy166II GTNNAC 2 cut(s) 165, 193
Hpy188I TCNGA 4 cut(s) 48, 123, 254, 454
Hpy8I GTNNAC 2 cut(s) 165, 193
HpyAV CCTTC 2 cut(s) 322, 331
HpyCH4IV ACGT 1 cut(s) 206
HpyCH4V TGCA 3 cut(s) 231, 308, 523
HpyF10VI GCNNNNNNNGC 1 cut(s) 183
HpyF3I CTNAG 1 cut(s) 453
HpySE526I ACGT 1 cut(s) 206
Hsp92II CATG 1 cut(s) 90
HspAI GCGC 2 cut(s) 16, 59
KroI GCCGGC 1 cut(s) 107
KroNI GCCGGC 1 cut(s) 109
Ksp22I TGATCA 1 cut(s) 249
Kzo9I GATC 4 cut(s) 249, 360, 408, 511
LmnI GCTCC 1 cut(s) 138
LpnPI CCDG 7 cut(s) 121, 182, 209, 279, 391, 460, 509
Lsp1109I GCAGC 1 cut(s) 292
MabI ACCWGGT 1 cut(s) 195
MaeII ACGT 1 cut(s) 206
MaeIII GTNAC 2 cut(s) 132, 209
MalI GATC 4 cut(s) 251, 362, 410, 513
MboI GATC 4 cut(s) 249, 360, 408, 511
MboII GAAGA 4 cut(s) 16, 365, 385, 485
MflI RGATCY 1 cut(s) 408
MluCI AATT 3 cut(s) 80, 322, 396
MlyI GAGTC 1 cut(s) 304
MnlI CCTC 5 cut(s) 38, 106, 262, 327, 430
MroNI GCCGGC 1 cut(s) 107
MseI TTAA 1 cut(s) 425
MspI CCGG 2 cut(s) 108, 447
MspR9I CCNGG 2 cut(s) 197, 447
MvaI CCWGG 1 cut(s) 197
MvnI CGCG 3 cut(s) 16, 21, 59
MwoI GCNNNNNNNGC 1 cut(s) 183
NaeI GCCGGC 1 cut(s) 109
NciI CCSGG 1 cut(s) 447
NdeI CATATG 1 cut(s) 469
NdeII GATC 4 cut(s) 249, 360, 408, 511
NgoMIV GCCGGC 1 cut(s) 107
NlaIII CATG 1 cut(s) 90
NlaIV GGNNCC 2 cut(s) 7, 410
NmuCI GTSAC 1 cut(s) 209
NspI RCATGY 1 cut(s) 90
PaeR7I CTCGAG 1 cut(s) 292
PciI ACATGT 1 cut(s) 86
PcsI WCGNNNNNNNCGW 1 cut(s) 224
PdiI GCCGGC 1 cut(s) 109
PkrI GCNGC 3 cut(s) 20, 73, 307
PleI GAGTC 1 cut(s) 303
PpsI GAGTC 1 cut(s) 303
PscI ACATGT 1 cut(s) 86
Psp6I CCWGG 1 cut(s) 195
PspGI CCWGG 1 cut(s) 195
PspN4I GGNNCC 2 cut(s) 7, 410
PspXI VCTCGAGB 1 cut(s) 292
PsuI RGATCY 1 cut(s) 408
RsaI GTAC 1 cut(s) 194
RsaNI GTAC 1 cut(s) 193
SaqAI TTAA 1 cut(s) 425
SatI GCNGC 3 cut(s) 19, 72, 306
Sau3AI GATC 4 cut(s) 249, 360, 408, 511
SchI GAGTC 1 cut(s) 304
ScrFI CCNGG 2 cut(s) 197, 447
SetI ASST 8 cut(s) 151, 198, 209, 273, 435, 441, 501, 528
SexAI ACCWGGT 1 cut(s) 195
Sfr274I CTCGAG 1 cut(s) 292
SlaI CTCGAG 1 cut(s) 292
SmlI CTYRAG 2 cut(s) 292, 434
SmoI CTYRAG 2 cut(s) 292, 434
Sse9I AATT 3 cut(s) 80, 322, 396
SsiI CCGC 6 cut(s) 19, 37, 69, 72, 177, 492
StyD4I CCNGG 2 cut(s) 195, 445
StyI CCWWGG 1 cut(s) 417
TaiI ACGT 1 cut(s) 209
TaqI TCGA 3 cut(s) 293, 298, 363
TasI AATT 3 cut(s) 80, 322, 396
TauI GCSGC 2 cut(s) 21, 74
Tru1I TTAA 1 cut(s) 425
Tru9I TTAA 1 cut(s) 425
TscAI CASTG 3 cut(s) 172, 252, 525
TseFI GTSAC 1 cut(s) 209
TseI GCWGC 1 cut(s) 305
Tsp45I GTSAC 1 cut(s) 209
TspDTI ATGAA 1 cut(s) 486
TspGWI ACGGA 2 cut(s) 105, 151
TspRI CASTG 3 cut(s) 172, 252, 525
XagI CCTNNNNNAGG 1 cut(s) 416
XapI RAATTY 3 cut(s) 80, 322, 396
XceI RCATGY 1 cut(s) 90
XhoI CTCGAG 1 cut(s) 292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.