pycom17g12960

Belongs to the enoyl-CoA hydratase isomerase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
N/A
Physical Location & Seq
Forward (+)
10667289 .. 10668265
977 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 411 bp
ATGGGTGTTTGCAAAATCTCTGATCTAGAGAAGCGTTTGATAAGCTTAAACTCTGGAAATGAGAATGCAGTCAAAGCTGCGATTGAAGAATTTTCTGTGGCAGTCAAGCCTGATGAAGAAAGTGTTTTAAACAAGAAGTCAGTAATTGATGGGTGCTTCTCCAAAGATACAGTGGCTGAGATTATACAATCGTTTGAAGCAGAAGCAAACAAAGAAGGAAATGGATGGATAGGTCCAGTTCTTAAGGGCTTAAAGAGGTCATCACCTACAGGATTGAGAATAACTCTACGATCGATTCGCGAAGGCAGGAAGCAAACATTGTCTGAATCTCTGACGAAGGAATTTAGACTCACAATCAATATATTAAGATCCACAATTTCTAAGGATGTTTATGAGGTAATTGTCGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009628 GO:0050896 GO:0080167
KEGG Pathways
Metabolic & Signaling
ko00280 ko00410 ko00640 ko01100 ko01200 map00280 map00410 map00640 map01100 map01200
Pfam Domains
Protein Families
ECH_2

Protein Analysis

137

Amino Acids

14.98

Weight (kDa)

8.56

Isoelectric Point (pI)

52.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ECH_2 PF16113 5 - 132 6.2e-24 Enoyl-CoA hydratase/isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 300
AclWI GGATC 1 cut(s) 363
AcsI RAATTY 2 cut(s) 89, 341
AflII CTTAAG 1 cut(s) 242
AgsI TTSAA 2 cut(s) 86, 197
AluBI AGCT 2 cut(s) 45, 77
AluI AGCT 2 cut(s) 45, 77
AlwI GGATC 1 cut(s) 363
AlwNI CAGNNNCTG 1 cut(s) 176
ApeKI GCWGC 1 cut(s) 77
ApoI RAATTY 2 cut(s) 89, 341
AspS9I GGNCC 1 cut(s) 233
AsuHPI GGTGA 1 cut(s) 255
AvaII GGWCC 1 cut(s) 233
BbvI GCAGC 1 cut(s) 64
BccI CCATC 2 cut(s) 143, 219
BfaI CTAG 1 cut(s) 26
BfmI CTRYAG 1 cut(s) 267
BfrI CTTAAG 1 cut(s) 242
BisI GCNGC 1 cut(s) 78
BlsI GCNGC 1 cut(s) 79
Bme18I GGWCC 1 cut(s) 233
BmgT120I GGNCC 1 cut(s) 233
BplI GAGNNNNNCTC 2 cut(s) 268, 300
Bsa29I ATCGAT 1 cut(s) 293
Bse1I ACTGG 1 cut(s) 236
BseCI ATCGAT 1 cut(s) 293
BseGI GGATG 2 cut(s) 230, 391
BseMII CTCAG 1 cut(s) 168
BseNI ACTGG 1 cut(s) 236
BseXI GCAGC 1 cut(s) 64
Bsh1236I CGCG 1 cut(s) 300
Bsh1285I CGRYCG 1 cut(s) 293
BshVI ATCGAT 1 cut(s) 293
BsiEI CGRYCG 1 cut(s) 293
BsmI GAATGC 1 cut(s) 70
Bsp143I GATC 3 cut(s) 22, 290, 368
Bsp68I TCGCGA 1 cut(s) 300
BspCNI CTCAG 1 cut(s) 169
BspDI ATCGAT 1 cut(s) 293
BspFNI CGCG 1 cut(s) 300
BspPI GGATC 1 cut(s) 363
BspTI CTTAAG 1 cut(s) 242
BsrI ACTGG 1 cut(s) 236
BssMI GATC 3 cut(s) 22, 290, 368
Bst4CI ACNGT 1 cut(s) 172
BstAFI CTTAAG 1 cut(s) 242
BstDEI CTNAG 2 cut(s) 177, 381
BstF5I GGATG 2 cut(s) 230, 391
BstFNI CGCG 1 cut(s) 300
BstKTI GATC 3 cut(s) 25, 293, 371
BstMBI GATC 3 cut(s) 22, 290, 368
BstMCI CGRYCG 1 cut(s) 293
BstMWI GCNNNNNNNGC 1 cut(s) 74
BstSFI CTRYAG 1 cut(s) 267
BstUI CGCG 1 cut(s) 300
BstV1I GCAGC 1 cut(s) 64
BstX2I RGATCY 1 cut(s) 368
BstYI RGATCY 1 cut(s) 368
Bsu15I ATCGAT 1 cut(s) 293
BsuTUI ATCGAT 1 cut(s) 293
BtsCI GGATG 2 cut(s) 230, 391
BtsIMutI CAGTG 1 cut(s) 177
BtuMI TCGCGA 1 cut(s) 300
CaiI CAGNNNCTG 1 cut(s) 176
Cfr13I GGNCC 1 cut(s) 233
ClaI ATCGAT 1 cut(s) 293
CviJI RGCY 5 cut(s) 45, 77, 109, 176, 249
CviKI_1 RGCY 5 cut(s) 45, 77, 109, 176, 249
DdeI CTNAG 2 cut(s) 177, 381
DpnI GATC 3 cut(s) 24, 292, 370
DpnII GATC 3 cut(s) 22, 290, 368
DraI TTTAAA 1 cut(s) 129
Eco47I GGWCC 1 cut(s) 233
FaiI YATR 4 cut(s) 185, 362, 393, 409
Fnu4HI GCNGC 1 cut(s) 78
FokI GGATG 2 cut(s) 237, 398
Fsp4HI GCNGC 1 cut(s) 78
FspBI CTAG 1 cut(s) 26
GluI GCNGC 1 cut(s) 78
HindIII AAGCTT 1 cut(s) 43
HinfI GANTC 3 cut(s) 295, 326, 348
HphI GGTGA 1 cut(s) 255
Hpy188I TCNGA 3 cut(s) 22, 325, 333
Hpy188III TCNNGA 3 cut(s) 26, 54, 299
HpyAV CCTTC 3 cut(s) 209, 296, 331
HpyCH4III ACNGT 1 cut(s) 172
HpyCH4V TGCA 2 cut(s) 12, 68
HpyF10VI GCNNNNNNNGC 1 cut(s) 74
HpyF3I CTNAG 2 cut(s) 177, 381
Kzo9I GATC 3 cut(s) 22, 290, 368
LpnPI CCDG 5 cut(s) 39, 123, 249, 255, 292
Lsp1109I GCAGC 1 cut(s) 64
MaeI CTAG 1 cut(s) 26
MalI GATC 3 cut(s) 24, 292, 370
MboI GATC 3 cut(s) 22, 290, 368
MboII GAAGA 2 cut(s) 98, 128
MflI RGATCY 1 cut(s) 368
MluCI AATT 5 cut(s) 89, 144, 341, 375, 399
MlyI GAGTC 1 cut(s) 342
MnlI CCTC 2 cut(s) 249, 388
MseI TTAA 5 cut(s) 47, 128, 243, 251, 365
MspCI CTTAAG 1 cut(s) 242
Mva1269I GAATGC 1 cut(s) 70
MvnI CGCG 1 cut(s) 300
MwoI GCNNNNNNNGC 1 cut(s) 74
NdeII GATC 3 cut(s) 22, 290, 368
NruI TCGCGA 1 cut(s) 300
PctI GAATGC 1 cut(s) 70
PfeI GAWTC 2 cut(s) 295, 326
PkrI GCNGC 1 cut(s) 79
Ple19I CGATCG 1 cut(s) 293
PleI GAGTC 1 cut(s) 342
PpsI GAGTC 1 cut(s) 342
PspPI GGNCC 1 cut(s) 233
PstNI CAGNNNCTG 1 cut(s) 176
PsuI RGATCY 1 cut(s) 368
PvuI CGATCG 1 cut(s) 293
RruI TCGCGA 1 cut(s) 300
SaqAI TTAA 5 cut(s) 47, 128, 243, 251, 365
SatI GCNGC 1 cut(s) 78
Sau3AI GATC 3 cut(s) 22, 290, 368
Sau96I GGNCC 1 cut(s) 233
SchI GAGTC 1 cut(s) 342
SetI ASST 6 cut(s) 47, 79, 235, 260, 268, 399
SfcI CTRYAG 1 cut(s) 267
SgeI CNNG 9 cut(s) 38, 66, 118, 122, 145, 248, 282, 311, 319
SinI GGWCC 1 cut(s) 233
SmlI CTYRAG 1 cut(s) 242
SmoI CTYRAG 1 cut(s) 242
Sse9I AATT 5 cut(s) 89, 144, 341, 375, 399
SspMI CTAG 1 cut(s) 26
TaaI ACNGT 1 cut(s) 172
TaqI TCGA 1 cut(s) 293
TasI AATT 5 cut(s) 89, 144, 341, 375, 399
TfiI GAWTC 2 cut(s) 295, 326
Tru1I TTAA 5 cut(s) 47, 128, 243, 251, 365
Tru9I TTAA 5 cut(s) 47, 128, 243, 251, 365
TscAI CASTG 1 cut(s) 177
TseI GCWGC 1 cut(s) 77
TspDTI ATGAA 1 cut(s) 129
TspRI CASTG 1 cut(s) 177
Vha464I CTTAAG 1 cut(s) 242
VpaK11BI GGWCC 1 cut(s) 233
XapI RAATTY 2 cut(s) 89, 341
XbaI TCTAGA 1 cut(s) 25
XcmI CCANNNNNNNNNTGG 1 cut(s) 169
XspI CTAG 1 cut(s) 26
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.