pycom17g13020

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
N/A
Physical Location & Seq
Forward (+)
10708892 .. 10710167
1276 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 522 bp
ATGGGGGTCGAACTTGCAGCACAATGCATAGATACTTCACCTTTCCTTTTCCATAACCCACTCTCCATCCCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCTCATCATGCAAACAAACCCAGAACAAAGAGAGAAGGAAGAAGAAGAACGAGGCGGTGCTGTTTCTGGTGCCAGTGCATTAATGACAGAGCACGGCGGCCTCGAGGAGATGGCTAAGGAAGTTGTGGCAGCAGAGGCTCCTCATCCATACGCTTTTCATGTATCCGGACCTCGCAATTTGATCTCTCCGAATTGGAGAGACCTCATAAACTCGAGTTGGAAGGACGGGAATTATAAACGAACTGTAATCGCGTGCTTCATACAAGCAATATATTTGCTTGAACTCGACAGACAAGAGAATCGAGATGAAGCACATGCTCTAGCTCCTAAATGGTGGATACCCTTTAAGTATAAATTGTCCCAAACCTTAATCGATGAAAGAGATGGATCCATATTTGGGGCAATACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

174

Amino Acids

19.32

Weight (kDa)

5.09

Isoelectric Point (pI)

35.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 345
AbsI CCTCGAGG 1 cut(s) 212
AccB1I GGYRCC 1 cut(s) 179
AccII CGCG 1 cut(s) 362
AccIII TCCGGA 1 cut(s) 275
AciI CCGC 2 cut(s) 165, 207
AclWI GGATC 2 cut(s) 492, 505
AfiI CCNNNNNNNGG 1 cut(s) 507
AgsI TTSAA 1 cut(s) 392
AluBI AGCT 1 cut(s) 434
AluI AGCT 1 cut(s) 434
Alw21I GWGCWC 1 cut(s) 204
Alw26I GTCTC 1 cut(s) 303
AlwI GGATC 2 cut(s) 492, 505
Ama87I CYCGRG 2 cut(s) 212, 322
Aor13HI TCCGGA 1 cut(s) 275
AoxI GGCC 1 cut(s) 208
ApeKI GCWGC 2 cut(s) 17, 239
AseI ATTAAT 1 cut(s) 191
AspS9I GGNCC 1 cut(s) 278
AsuHPI GGTGA 1 cut(s) 30
AvaI CYCGRG 2 cut(s) 212, 322
AvaII GGWCC 1 cut(s) 278
BamHI GGATCC 1 cut(s) 497
BanI GGYRCC 1 cut(s) 179
Bbv12I GWGCWC 1 cut(s) 204
BbvI GCAGC 2 cut(s) 29, 251
BccI CCATC 3 cut(s) 74, 214, 488
BceAI ACGGC 1 cut(s) 220
BciVI GTATCC 2 cut(s) 283, 441
BcoDI GTCTC 1 cut(s) 303
BfaI CTAG 1 cut(s) 431
BfuI GTATCC 2 cut(s) 283, 441
BisI GCNGC 3 cut(s) 18, 208, 240
BlsI GCNGC 3 cut(s) 19, 209, 241
Bme18I GGWCC 1 cut(s) 278
BmeT110I CYCGRG 2 cut(s) 212, 322
BmgT120I GGNCC 1 cut(s) 278
BmiI GGNNCC 3 cut(s) 181, 249, 499
Bpu10I CCTNAGC 1 cut(s) 225
Bsa29I ATCGAT 1 cut(s) 483
BsaI GGTCTC 1 cut(s) 303
BsaWI WCCGGW 1 cut(s) 275
BsaXI ACNNNNNCTCC 2 cut(s) 47, 77
Bsc4I CCNNNNNNNGG 1 cut(s) 507
Bse1I ACTGG 1 cut(s) 183
BseAI TCCGGA 1 cut(s) 275
BseCI ATCGAT 1 cut(s) 483
BseGI GGATG 2 cut(s) 66, 253
BseLI CCNNNNNNNGG 1 cut(s) 507
BseNI ACTGG 1 cut(s) 183
BseRI GAGGAG 3 cut(s) 101, 230, 240
BseXI GCAGC 2 cut(s) 29, 251
Bsh1236I CGCG 1 cut(s) 362
BshFI GGCC 1 cut(s) 210
BshNI GGYRCC 1 cut(s) 179
BshVI ATCGAT 1 cut(s) 483
BsiHKAI GWGCWC 1 cut(s) 204
BsiHKCI CYCGRG 2 cut(s) 212, 322
BsiSI CCGG 1 cut(s) 276
BslFI GGGAC 1 cut(s) 454
BslI CCNNNNNNNGG 1 cut(s) 507
BsmAI GTCTC 1 cut(s) 303
BsmFI GGGAC 1 cut(s) 454
BsnI GGCC 1 cut(s) 210
Bso31I GGTCTC 1 cut(s) 303
BsoBI CYCGRG 2 cut(s) 212, 322
Bsp1286I GDGCHC 1 cut(s) 204
Bsp13I TCCGGA 1 cut(s) 275
Bsp143I GATC 2 cut(s) 291, 497
BspACI CCGC 2 cut(s) 165, 207
BspANI GGCC 1 cut(s) 210
BspDI ATCGAT 1 cut(s) 483
BspEI TCCGGA 1 cut(s) 275
BspFNI CGCG 1 cut(s) 362
BspLI GGNNCC 3 cut(s) 181, 249, 499
BspPI GGATC 2 cut(s) 492, 505
BspT107I GGYRCC 1 cut(s) 179
BspTNI GGTCTC 1 cut(s) 303
BsrI ACTGG 1 cut(s) 183
BssMI GATC 2 cut(s) 291, 497
Bst4CI ACNGT 2 cut(s) 355, 519
BstC8I GCNNGC 1 cut(s) 364
BstDEI CTNAG 1 cut(s) 225
BstF5I GGATG 2 cut(s) 66, 253
BstFNI CGCG 1 cut(s) 362
BstKTI GATC 2 cut(s) 294, 500
BstMAI GTCTC 1 cut(s) 303
BstMBI GATC 2 cut(s) 291, 497
BstMWI GCNNNNNNNGC 1 cut(s) 245
BstNSI RCATGY 1 cut(s) 428
BstUI CGCG 1 cut(s) 362
BstV1I GCAGC 2 cut(s) 29, 251
BstX2I RGATCY 1 cut(s) 497
BstYI RGATCY 1 cut(s) 497
Bsu15I ATCGAT 1 cut(s) 483
BsuI GTATCC 2 cut(s) 283, 441
BsuRI GGCC 1 cut(s) 210
BsuTUI ATCGAT 1 cut(s) 483
BtsCI GGATG 2 cut(s) 66, 253
BtsIMutI CAGTG 1 cut(s) 190
Cac8I GCNNGC 1 cut(s) 364
Cfr13I GGNCC 1 cut(s) 278
ClaI ATCGAT 1 cut(s) 483
CviAII CATG 3 cut(s) 118, 269, 425
CviJI RGCY 4 cut(s) 210, 224, 248, 434
CviKI_1 RGCY 4 cut(s) 210, 224, 248, 434
DdeI CTNAG 1 cut(s) 225
DpnI GATC 2 cut(s) 293, 499
DpnII GATC 2 cut(s) 291, 497
Eco31I GGTCTC 1 cut(s) 303
Eco47I GGWCC 1 cut(s) 278
Eco88I CYCGRG 2 cut(s) 212, 322
EcoT22I ATGCAT 1 cut(s) 29
FaeI CATG 3 cut(s) 121, 272, 428
FaqI GGGAC 1 cut(s) 454
FatI CATG 3 cut(s) 117, 268, 424
Fnu4HI GCNGC 3 cut(s) 18, 208, 240
FokI GGATG 2 cut(s) 53, 240
Fsp4HI GCNGC 3 cut(s) 18, 208, 240
FspBI CTAG 1 cut(s) 431
GluI GCNGC 3 cut(s) 18, 208, 240
HaeIII GGCC 1 cut(s) 210
HapII CCGG 1 cut(s) 276
Hin1II CATG 3 cut(s) 121, 272, 428
HinfI GANTC 1 cut(s) 409
HpaII CCGG 1 cut(s) 276
HphI GGTGA 1 cut(s) 30
Hpy188I TCNGA 1 cut(s) 300
Hpy188III TCNNGA 2 cut(s) 276, 413
HpyAV CCTTC 2 cut(s) 139, 325
HpyCH4III ACNGT 2 cut(s) 355, 519
HpyCH4V TGCA 4 cut(s) 17, 27, 121, 188
HpyF10VI GCNNNNNNNGC 1 cut(s) 245
HpyF3I CTNAG 1 cut(s) 225
Hsp92II CATG 3 cut(s) 121, 272, 428
Kpn2I TCCGGA 1 cut(s) 275
Kzo9I GATC 2 cut(s) 291, 497
LmnI GCTCC 2 cut(s) 253, 439
LpnPI CCDG 4 cut(s) 144, 162, 196, 289
Lsp1109I GCAGC 2 cut(s) 29, 251
MaeI CTAG 1 cut(s) 431
MalI GATC 2 cut(s) 293, 499
MboI GATC 2 cut(s) 291, 497
MboII GAAGA 3 cut(s) 161, 164, 167
MflI RGATCY 1 cut(s) 497
MhlI GDGCHC 1 cut(s) 204
MluCI AATT 4 cut(s) 286, 301, 340, 464
MmeI TCCRAC 1 cut(s) 308
MnlI CCTC 9 cut(s) 81, 122, 155, 208, 221, 238, 261, 291, 323
Mph1103I ATGCAT 1 cut(s) 29
MroI TCCGGA 1 cut(s) 275
MseI TTAA 3 cut(s) 191, 456, 479
MspI CCGG 1 cut(s) 276
MvnI CGCG 1 cut(s) 362
MwoI GCNNNNNNNGC 1 cut(s) 245
NdeII GATC 2 cut(s) 291, 497
NlaIII CATG 3 cut(s) 121, 272, 428
NlaIV GGNNCC 3 cut(s) 181, 249, 499
NsiI ATGCAT 1 cut(s) 29
NspI RCATGY 1 cut(s) 428
PaeR7I CTCGAG 2 cut(s) 212, 322
PcsI WCGNNNNNNNCGW 1 cut(s) 210
PfeI GAWTC 1 cut(s) 409
PkrI GCNGC 3 cut(s) 19, 209, 241
PshBI ATTAAT 1 cut(s) 191
PsiI TTATAA 1 cut(s) 345
PspN4I GGNNCC 3 cut(s) 181, 249, 499
PspPI GGNCC 1 cut(s) 278
PspXI VCTCGAGB 2 cut(s) 212, 322
PsuI RGATCY 1 cut(s) 497
SaqAI TTAA 3 cut(s) 191, 456, 479
SatI GCNGC 3 cut(s) 18, 208, 240
Sau3AI GATC 2 cut(s) 291, 497
Sau96I GGNCC 1 cut(s) 278
SduI GDGCHC 1 cut(s) 204
SetI ASST 5 cut(s) 43, 283, 315, 436, 479
Sfr274I CTCGAG 2 cut(s) 212, 322
SinI GGWCC 1 cut(s) 278
SlaI CTCGAG 2 cut(s) 212, 322
SmlI CTYRAG 2 cut(s) 212, 322
SmoI CTYRAG 2 cut(s) 212, 322
Sse9I AATT 4 cut(s) 286, 301, 340, 464
SsiI CCGC 2 cut(s) 165, 207
SspMI CTAG 1 cut(s) 431
TaaI ACNGT 2 cut(s) 355, 519
TaqI TCGA 6 cut(s) 9, 213, 323, 396, 412, 483
TasI AATT 4 cut(s) 286, 301, 340, 464
TauI GCSGC 1 cut(s) 210
TfiI GAWTC 1 cut(s) 409
Tru1I TTAA 3 cut(s) 191, 456, 479
Tru9I TTAA 3 cut(s) 191, 456, 479
TscAI CASTG 1 cut(s) 190
TseI GCWGC 2 cut(s) 17, 239
TspDTI ATGAA 4 cut(s) 257, 358, 432, 501
TspRI CASTG 1 cut(s) 190
VpaK11BI GGWCC 1 cut(s) 278
VspI ATTAAT 1 cut(s) 191
XceI RCATGY 1 cut(s) 428
XhoI CTCGAG 2 cut(s) 212, 322
XspI CTAG 1 cut(s) 431
Zsp2I ATGCAT 1 cut(s) 29
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.